- Research article
- Open Access
Whitefly (Bemisia tabaci) genome project: analysis of sequenced clones from egg, instar, and adult (viruliferous and non-viruliferous) cDNA libraries
© Leshkowitz et al; licensee BioMed Central Ltd. 2006
- Received: 21 November 2005
- Accepted: 11 April 2006
- Published: 11 April 2006
The past three decades have witnessed a dramatic increase in interest in the whitefly Bemisia tabaci, owing to its nature as a taxonomically cryptic species, the damage it causes to a large number of herbaceous plants because of its specialized feeding in the phloem, and to its ability to serve as a vector of plant viruses. Among the most important plant viruses to be transmitted by B. tabaci are those in the genus Begomovirus (family, Geminiviridae). Surprisingly, little is known about the genome of this whitefly. The haploid genome size for male B. tabaci has been estimated to be approximately one billion bp by flow cytometry analysis, about five times the size of the fruitfly Drosophila melanogaster. The genes involved in whitefly development, in host range plasticity, and in begomovirus vector specificity and competency, are unknown.
To address this general shortage of genomic sequence information, we have constructed three cDNA libraries from non-viruliferous whiteflies (eggs, immature instars, and adults) and two from adult insects that fed on tomato plants infected by two geminiviruses: Tomato yellow leaf curl virus (TYLCV) and Tomato mottle virus (ToMoV). In total, the sequence of 18,976 clones was determined. After quality control, and removal of 5,542 clones of mitochondrial origin 9,110 sequences remained which included 3,843 singletons and 1,017 contigs. Comparisons with public databases indicated that the libraries contained genes involved in cellular and developmental processes. In addition, approximately 1,000 bases aligned with the genome of the B. tabaci endosymbiotic bacterium Candidatus Portiera aleyrodidarum, originating primarily from the egg and instar libraries. Apart from the mitochondrial sequences, the longest and most abundant sequence encodes vitellogenin, which originated from whitefly adult libraries, indicating that much of the gene expression in this insect is directed toward the production of eggs.
This is the first functional genomics project involving a hemipteran (Homopteran) insect from the subtropics/tropics. The B. tabaci sequence database now provides an important tool to initiate identification of whitefly genes involved in development, behaviour, and B. tabaci-mediated begomovirus transmission.
- Gene Ontology
- Tomato Yellow Leaf Curl Virus
- Mitochondrial Sequence
- Directional cDNA Library
- Acetylcholine Receptor Alpha Subunit
The past three decades have witnessed a dramatic increase in the economic importance of the whitefly Bemisia tabaci (Genn.) (Aleyrodidae; Hemiptera) in subtropical and mild temperate agriculture systems, owing to the damage it causes to plants when it feeds in the phloem, and its ability to transmit plant viruses. B. tabaci occupies tropical and subtropical habitats, producing 11–15 generations per year [1, 2]. The B. tabaci complex consists of diverse biological 'types' with distinct genetic polymorphisms [3, 4], and differences in host range, fecundity, dispersal behaviours, prokaryotic endosymbiont composition, and competency with respect to begomovirus transmission, a group of small circular ssDNA plant viruses (genus Begomovirus, family Geminiviridae) [5, 6]. The highly fecund Old World B biotype can produce ~ 300 eggs/female, colonizes over 500 host species, while the New World A type colonizes about 200 species and has a lower fecundity (~ 100 eggs/female). In contrast, the Jatropha type colonizes only a few species within the genus Jatropha and exhibits low fecundity (~ 30–50 eggs/female) .
B. tabaci adults develop from eggs, after passing through four instars in approximately 2–3 wk and development is temperature dependent. Members of this complex are haplodiploid and thus unfertilized eggs give rise to haploid males; fertilized eggs develop into diploid females (arrhenotoky) [1, 2].
The B type of B. tabaci transmits begomoviruses to a large number of crop, ornamental, and weed species . Begomovirus have either one (monopartite) or two (bipartite) genomic components . Those infecting tomato constitute a large group of begomoviruses. Among them the bipartite Tomato mottle virus (ToMoV) originated in the New World (Florida/Caribbean region), whereas, the monopartite Old World Tomato yellow leaf curl virus (TYLCV) is indigenous to the Old World (Middle East and Africa). TYLCV recently was introduced to the Caribbean Islands and has since spread into the South eastern states of the U.S.A. .
Begomoviruses are transmitted by B. tabaci in a circulative manner [10, 11]. Virus particles ingested through the stylets enter the oesophagus and the filter chamber, are transported through the gut into the hemocoel, reach the salivary glands and are finally 'transmitted' during feeding, about 8–12 h after the beginning of an acquisition access period . Velocity of translocation is reported to constitute an intrinsic property of the vector, not of the virus [12, 13]. B. tabaci is able to transmit begomoviruses, and in particular TYLCV, for its lifetime, after the latent period has been achieved [14, 15]. The ingestion of TYLCV by the whitefly vector is accompanied by a marked decrease in whitefly longevity and fertility . In contrast whiteflies that have ingested ToMoV displayed higher fecundity when reared on virus-free tomato than whiteflies not exposed to the virus . TYLCV transcripts have been found in B. tabaci harbouring this virus, whereas viral transcripts are not detected in whiteflies that have ingested ToMoV , suggesting a fundamental difference in interactions between these two begomoviruses and their whitefly vector.
At least one whitefly species that colonizes some of the same hosts as B. tabaci (e.g. the greenhouse whitefly, Trialeurodes vaporariorum) is known to be capable of ingesting, but does not transmit begomoviruses , and at least one barrier to transmission has been shown to occur at the gut/hemocoel interface [12, 18]. The receptors that are hypothesized to mediate begomovirus translocation into the salivary glands of B. tabaci, which is a requisite to transmission, and their genes, are presently unidentified.
Surprisingly very little is known about the genetic make up of this insect. The nuclear DNA content of B. tabaci male and female was estimated as 1.04 and 2.06 pg respectively, using flow cytometry, indicating that the haploid genome of B. tabaci contains about one billion bp, which is approximately five times the size of the genome of the fruitfly Drosophila melanogaster . However, it is still not clear if this size estimate will prove to be accurate and so a long-term goal is to determine the complete genome of this whitefly. Ultimately it is of interest to isolate and identify the genes expressed during the life cycle of the whitefly B. tabaci and to understand the genetic makeup of this pest. Of particular interest is the identification of specific genes and their functions, which are expressed during the development of B. tabaci, as well as those involved in circulative virus transmission, the detoxification of insecticides, and the determination of polyphagy or monophagy in different B. tabaci biotypes. Consequently, the construction of cDNA libraries and the analyses of the sequences for the widespread 'B' biotype of B. tabaci constitute a first step in this endeavour.
Preparation of cDNA libraries
Five independent cDNA, or expressed sequence tag (EST), libraries were prepared from mRNA isolated from adult B. tabaci 'B' biotype whiteflies: (1) reared in Israel on cotton plants [a non-host of TYLCV/ToMoV (= HBT)], or reared in Florida, USA on (2) TYLCV-infected tomato plants (= TYLCV), and (3) ToMoV-infected tomato plants (= TOMOV). Libraries also were constructed from mRNA isolated from the eggs of non-viruliferous whiteflies (= EGG), and immature instars (crawler to pupae) of non-viruliferous insects (= INST). The libraries were not normalized or amplified. Among them the bipartite Tomato mottle virus (ToMoV) originated in the New World (Florida/Caribbean region), whereas, the monopartite Tomato yellow leaf curl virus (TYLCV) is indigenous to the Old World (Middle East and Africa).
Assessment of library and sequence quality
Number of sequences from the various libraries and number of sequences assembled into contigs (sequences of mitochondrial origin were removed from the contig assembly process)
Number of sequences
Total number of sequenced clones
Clones of mitochondrial origin
Sequences in contigs and singletons
EST assembly into contigs
Contig and singleton statistics. Breakdown with respect to their number. GC content, average length and % annotated are shown. Annotation was determined by having a homolog in any of the databases searched with an E-value of 1.0e-06. Even though the Candidatus Portiera aleyrodidarum bacterial DNA was present in the assembly processes, the sequences were removed when statistical calculations were carried out (represented by *) to avoid distortion of the results
Number of clones
Number of contigs
Number of singletons
Information on the most abundant contigs. The number of sequences that compose the contigs and the source library, their length, GC content and annotation are indicated. The contig with the highest number of sequences was identified as part of the B. tabaci primary symbiont, Candidatus Portiera aleyrodidarum, based on a partial genomic sequence (gi|32423678|gb|AY268081.1|). This DNA sequence was included in the assembly process. Among the most the abundant contigs were B. tabaci mitochondrial genome sequences (most of them were removed during the preassembly stage)
Number of sequences
Large subunit rRNA
Novel (signal peptide & transmembranal domain)
The second largest contig was composed of sequences homologous to the published B. tabaci mitochondrial genome (AY521259). The number of mitochondrial DNA clones was extremely high. We performed an initial screening for mitochondrial sequences by running RepeatMasker against the published mitochondrial genome (see Methods: analysis of library quality) using a threshold of up to 10% substitutions in matching DNA region or a Smith – Waterman score of at least 2500. For initial selection we preferred not to use stringent criteria in order not to leave aside nuclear genes and to allow assembly of contigs, a process that would not succeed without this screening. This first screening allowed eliminating 4,631 mitochondrial clones. Analysis of the remaining clones revealed the presence of 911 additional mitochondrial sequences (E value smaller or equal to e-06), 521 singletons and 41 contigs. Out of a total of 5,542 sequences, 4869 matched with sequences located between nucleotides 9,450 and 11,700 of the mitochondrial genome. This region contains the large subunit ribosomal RNA and three tRNA genes. It is possible that these sequences allowed RNA:tRNA dimer formation and initiation of cDNA synthesis as described in some retroviruses . The mitochondrial sequences could be used to study metabolism in microarray experiments. The number of sequences that are not mitochondrial or Candidatus Portiera aleyrodidarum from the various libraries is as follows: EGG: 357, HBT:1873, INST:1529, TOMOV:1985 and TYLCV:2367 (submitted to dbEST Genbank).
Identification of whitefly contigs and singletons by BLAST analysis
Number of contigs (out of the 4,860) that had a significant hit (E-value equal or smaller than 1.0e-06) with the listed databases
Number of contigs and singletons
Contigs and singletons found in at least one of the above databases
Sequences with no identifiable homology
No homologous sequences could be found for 2,649 (54.5%) of the contigs and singletons among the databases searched. The singletons showed a higher occurrence of lack of homology (58%). Because the library was poly(dT)-primed, some of these sequences may represent 3' untranslated regions (3' UTRs). It is also possible that the putative homologous regions are too short to produce a significant alignment.
Comparison to existing B. tabaci sequences in NCBI
There were 448 contigs and singletons presenting high similarity (E-value equal or smaller than 1.e-40) with B. tabaci DNA sequences. The majority of these hits (399) were to the whitefly mitochondria genome (AY521257.1), and represented mitochondrial sequences that were not removed in the preassembly process. The BLAST search against the EST database did not reveal any ESTs originating from B. tabaci (currently there are no B. tabaci ESTs in Genbank). Thus, homology searches indicated that the majority of the contigs and singletons described herein are novel B. tabaci genes, which are not known in the NCBI sequence databases.
Assignment of the whitefly contigs and singletons to common Gene Ontology terms
Comparing whitefly Gene Ontology to Drosophila
Drosophila homologs used to discover over-represented ontologies in the B. tabaci contigs and singletons
cytosolic ribosome (sensu Eukaryota)
structural constituent of ribosome
large ribosomal subunit
small ribosomal subunit
intracellular non-membrane-bound organelle
mitochondrial inner membrane
mitochondrial electron transport chain
ATP synthesis coupled electron transport
cellular physiological process
cytosolic large ribosomal subunit (sensu Eukaryota)
Structural molecule activity
eukaryotic 43S preinitiation complex
cellular macromolecule metabolism
monovalent inorganic cation transporter activity
Hydrogen ion transporter activity
generation of precursor metabolites and energy
cytosolic small ribosomal subunit (sensu Eukaryota)
eukaryotic 48S initiation complex
cellular protein metabolism
primary active transporter activity
intracellular membrane-bound organelle
Ribosome mitochondrial andmetabolism enriched
Drosophila homologs used to discover under-represented ontologies in the B. tabaci contigs and singletons
signal transducer activity
transmembrane receptor activity
Mapping whitefly contigs and singletons to pathways
Mapping the pathways for the 37 unique EC numbers extracted by the BiocloneDB application for the nr homologs. The output was produced by the 'KEGG gpath tool'.
map00010 Glycolysis / Gluconeogenesis
map00030 Pentose phosphate pathway
map00031 Inositol metabolism
map00040 Pentose and glucuronate interconversions
map00051 Fructose and mannose metabolism
map00052 Galactose metabolism
map00053 Ascorbate and aldarate metabolism
map00071 Fatty acid metabolism
map00120 Bile acid biosynthesis
map00130 Ubiquinone biosynthesis
map00150 Androgen and estrogen metabolism
map00190 Oxidative phosphorylation
map00193 ATP synthesis
map00220 Urea cycle and metabolism of amino groups
map00230 Purine metabolism
map00240 Pyrimidine metabolism
map00251 Glutamate metabolism
map00252 Alanine and aspartate metabolism
map00260 Glycine, serine and threonine metabolism
map00280 Valine, leucine and isoleucine degradation
map00310 Lysine degradation
map00330 Arginine and proline metabolism
map00340 Histidine metabolism
map00380 Tryptophan metabolism
map00400 Phenylalanine, tyrosine and tryptophan biosynthesis
map00410 beta-Alanine metabolism
map00480 Glutathione metabolism
map00500 Starch and sucrose metabolism
map00520 Nucleotide sugars metabolism
map00530 Aminosugars metabolism
map00550 Peptidoglycan biosynthesis
map00561 Glycerolipid metabolism
map00564 Glycerophospholipid metabolism
map00620 Pyruvate metabolism
map00630 Glyoxylate and dicarboxylate metabolism
map00631 1,2-Dichloroethane degradation
map00640 Propanoate metabolism
map00650 Butanoate metabolism
map00710 Carbon fixation
map00720 Reductive carboxylate cycle (CO2 fixation)
map00860 Porphyrin and chlorophyll metabolism
map00903 Limonene and pinene degradation
map00910 Nitrogen metabolism
map00970 Aminoacyl-tRNA biosynthesis
map02040 Flagellar assembly
map03020 RNA polymerase
map03070 Type III secretion system
Multiple alignments of vitellogenin-like contigs
Results of nine contigs BLAST searched against gi|2522237|dbj|BAA22791.1| vitellogenin from the butterfly Athalia rosae
The whitefly contig and EST database
A relational database with a web-based front end (WhiteFlyDB) was created to store, navigate, annotate and retrieve sequence and contig information . This database is based on the BioCloneDB application . The database contains all the relevant contig information such as the names of the sequence that compose it, the top hits against the described databases and the information extracted for these top hits (GO, EC, cellular location) as well as information on the homology itself. The sequences in fasta format and the tab delimited BLAST reports can be easily extracted and imported to Excel files.
The whitefly B. tabaci is a major pest to agricultural crops because it causes damage due to feeding and because it transmits many important viruses to plant species cultivated for food and fiber nearly worldwide. Previous to the present research and despite the importance of B. tabaci, the sequence of only a handful of mRNAs (mostly partial) encoding a handful of nuclear protein-coding genes has been published in Genbank. They include sequences encoding actins, a para-sodium channel, putative knottins, a NADP-dependent ketose reductase, two heat shock proteins, a nicotinic acetylcholine receptor alpha subunit, an acetyl cholinesterase-like protein, and a diffusible secreted glycoprotein. The results described in this communication represent the first attempt to develop a functional genomics program involving a homopteran species.
Since the amount of total RNA that could be extracted from eggs and instars was extremely low, we have not isolated polyA+-RNA, which has inevitably reduced the mRNA representation in the sample. Instead we have used total RNA as template for synthesizing cDNA. Libraries have been prepared from another insect pest, the brown citrus aphid Toxoptera citricida starting from RNA samples enriched in polyA+-RNA . However, it has to be noted that an adult aphid weighs approximately 300 micrograms, while an adult whitefly weighs approximately 30 micrograms. Moreover, the weight of a whitefly egg is approximately 1/1000 that of an adult. We have not normalized the libraries, a fact that allowed us to roughly estimate and compare the levels of expression of major genes in the different libraries.
The fraction of the expressed whitefly genes present in our database can be roughly estimated. Although the genome size of B. tabaci was estimated to be approximately five times that of Drosophila , it is logical to speculate that the two insect species may have approximately the same number of protein-encoding nuclear genes. The whitefly database contains the sequences of 975 contigs and 3,322 singletons (non-mitochondrial and non-bacterial). If we take into account that each contig represents a transcript of a single protein-coding nuclear gene our sequences represent 4,297 genes. The number of gene families (protein families) in Drosophila has been estimated as 674 and the number of genes not member of a gene family has been estimated as 10,786; altogether 11,460 protein-encoding genes . Hence the B. tabaci database may represent approximately one third of the insect nuclear protein-encoding genes. Additional sequencing from the 3' end of the clones may provide a more accurate estimation.
Within this whitefly database approximately half of the sequences had a match with an E value of at least 1.0e-06 to one of the databases; 1,544 sequences had a homology to a protein in the nr database. Approximately 60% of the whitefly contigs presented homologies with sequenced genomes of other insect species. No homologous sequence could be found for 2,649 contigs and singletons (54.5%) with any of the databases searched.
It was notable that the most abundant contig was vitellogenin. This ancient protein is the major yolk protein of eggs, where it is used as a food source during embryogenesis . There are three vitellogenin genes in Drosophila . The whitefly vitellogenin sequences were found exclusively in libraries from adult whiteflies, indicating that a relatively large amount of resources transcriptional activity is mobilized towards the production of eggs.
The database developed in this study provides a large source of information for studies of whitefly development, circulative transmission of begomoviruses, and choice of host plant. Comparing the sequences present in the various libraries may provide preliminary information on genes expressed during acquisition and transmission of begomoviruses, and ultimately those involved in B. tabaci development.
The set of sequences developed in this study makes available the first DNA sequence database for an important hemipteran (homopteran) pest of agricultural crops for the scientific community. Its availability will allow the investigation of important questions regarding whitefly biology, development, gene expression, and comparative biology. It will also facilitate studies to elucidate the genetics underlying gene expression in pest- and non-pest biotypes, and the basis for virus-vector specificity, resistance to insecticides, and plant host preferences for this cryptic species. This sequence set has been arrayed in a microchip format and enables biologically-based questions to be addressed by examining gene functionalities and expression patterns of the whitefly genome.
Libraries from adult viruliferous and non-viruliferous whiteflies
Directional cDNA libraries were constructed in the Lambda Uni-ZAP® XR vector using 5 μg of total RNA according to the manufacturers' instructions (Stratagene, La Jolla, CA). Whiteflies (B. tabaci, B biotype) were reared on TYLCV-infected tomato plants, on ToMoV-infected tomato plants and on non-infected tomato plants. The plants were kept in insect-proof cages. Insects were collected randomly from each of the colonies and placed at -80°C. Total RNA was isolated from pools of approximately 200 insects using the RNeasy Midi kit (QIAGEN). Contaminating DNA was discarded using RNAase-free DNAse I. Double stranded cDNA was synthesized using the cDNA Synthesis Kit (Stratagene) with primers containing each Eco RI and Xho I sites. Blunt ends were created, ligated to Eco RI adaptors, phosphorylated and digested with Eco RI. The cDNAs were size-fractionated using Drip Columns to obtain molecules longer than 300 bp. The cDNA was ligated to the Zap Express vector and used to transform E. coli cells in the presence of kanamycin. The phagemid pBK-CMV was packaged using the Gigapack III Gold packaging (Stratagene). Phages were used to inoculate XL1-Blue MRF cells. A library was generated with a titer of 5.2 × 1010 plaque-forming units per ml. The library was mass excised using Ex-Assist® helper phage (Stratagene) and bacterial clones containing excised pBluescript SK+ phagemids were recovered by random colony selection.
Libraries from eggs and instars
Directional cDNA libraries were constructed using the Creator SMART cDNA Library Construction Kit (Clontech). Eggs and instars were collected from leaves of cotton plants caged with whiteflies (B. tabaci, B biotype) and held in insect-proof cages. RNA was isolated using TRIZOL and Phase Lock Gel-Heavy tubes. The first strand cDNA was synthesized using the total RNA and the CDS III/3' PCR Primer which contains a Sfi IB site. The cDNA was amplified by PCR: the first-strand cDNA was used together with the 5' PCR primer which contains a Sfi IA site and the CDS III/3' PCR primer. Following phenol treatment, the DNA was digested with Sfi I and size-fractionated using CHROMA SPIN-400 Column. The high molecular weight cDNA fractions (with sticky ends) were pooled together and ligated with dephosphorylated pDNR-LIB vector treated Sfi IA and Sfi IB. The recombinant plasmids were electroporated into DH5α and 10G competent cells, and plated on LB agar plate containing chloramphenicol.
Plasmid clones were isolated from 1.7 ml overnight Luria-Bertani broth cultures using a Qiagen 9600 robot and Qiaprep 96 turbo plasmid isolation kits (Qiagen, Valencia, CA). Plasmid DNA (80 ng) was used as a template for ABI Prism® BigDye™ terminator cycle sequencing (PE Applied Biosystems, Foster City, CA.). Sequencing of the adult whitefly libraries was from the 5' end of the cloned cDNA using a T3 promoter universal primer: 5'ATTAACCCTCACTAAAGGGA3'. Sequencing of the egg and instar whitefly libraries was from the 5' end of the cloned cDNA using a M13 primer: 5'GAAGTTATCAGTCGACGG-3'. Reactions were concentrated and washed by ethanol precipitation. Pellets were resuspended in 15 μl of formamide prior to separation on an ABI Prism 3700 Sequencer (USHRL/ARS/USDA Genomics Laboratory, Fort Pierce FL).
Analysis of library quality
Mitochondrial sequences were not considered for clustering. Significant homology to the B. tabaci mitochondrion, complete genome (NC_006279.1) was found using cross_match . Of the 18,900 sequences analyzed 5,542 were found to contain significant mitochondrial sequences. However, as further analysis revealed the sequences that passed this filtering still contained some mitochondrial contamination.
Analysis and assembly of sequence data
Analysis of the chromatograms was done using Staden pregap4  and the following integrated programs: Phred, cross_match and RepeatMasker . Passed sequences were required to be longer than 75 bases after the trimming procedure. Cross_match and RepeatMasker were used to detect additional vector and adapter contamination after the sequence vector clip. A script was written for trimming the vector from the sequence ends, according to the information obtained by cross_match. Assembly of the contigs was carried out using the Staden gap4 normal assembly feature. Assembled contigs were used to perform BLAST searches (BLASTX and BLASTN) locally using the NCBI-BLAST  against the non-redundant protein sequence database, Drosophila proteins (dmel-translation), Swiss-Prot and EST_other (released March-May 2005), which were downloaded from the NCBI  or the FlyBase  databases.
The database application BiocloneDB  was used to manage the BLAST run, and to parse homologue alignment information, whereby an E-value of 1e-06 was used as a maximum cut-off. This information was stored in the SQL database. The DB application also supports sequence and contig queries and down-loads through the web interface .
Electronic annotation of contigs and singletons
The BiocloneDB application was used to extract from the EBI SRS information regarding the closest annotated homolog of contigs and singletons, including the GO annotation , EC number  and cellular location. The ontology distribution, according to the Swiss-Prot homologous proteins, was determined using the FatiGO tool . KEGG tool  was used to find the pathway distribution with the nr homologies, which contain an EC number. Over- and under-represented ontologies, with respect to the Drosophila genome, were Drosophila melanogaster homologies using the GOstat tool .
Multiple sequence alignment of the 5 translated contigs which their best homology was to Vitellogenin precursor from A. rosae (BAA22791.1) and their homology was to the same region in the A. rosae protein ranging from amino acid 1400-1920. The alignment was done by VectorNTI AlignX tool using the A. rosae protein as a profile. The MSF file produced is displayed using the Mview tool .
Other bioinformatic tools
Translation view was accomplished using the Prettyseq tool and percent GC calculations were carried out using Geecee. Both tools are part of the EMBOSS suite .
This research was supported by research grant number 593/02-1 from The Israel Science Foundation (ISF) to HC and by research grant IS-3479-03 from The United States – Israel Binational Agricultural Research and Development Fund (BARD) to HC, JKB, RS, and CM. DL was supported by grant #1424 of the Israeli Ministry of Science to COBI (Center of Knowledge Bioinformatics Infrastructure), as part of the Bioinformatics unit of the Hebrew University of Jerusalem. We thank Dvorah Weisman for computer resource support.
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