Research article
Open Access

Structure and properties of transcriptional networks driving selenite stress response in yeasts

  • Hélène Salin†1, 3,
  • Vivienne Fardeau†1, 4,
  • Eugenia Piccini1,
  • Gaelle Lelandais1, 5,
  • Véronique Tanty2,
  • Sophie Lemoine2,
  • Claude Jacq1 and
  • Frédéric Devaux1Email author
†Contributed equally
BMC Genomics20089:333

https://doi.org/10.1186/1471-2164-9-333

©  Salin et al; licensee BioMed Central Ltd. 2008

Received: 09 June 2008

Accepted: 15 July 2008

Published: 15 July 2008

Abstract

Background

Stress responses provide valuable models for deciphering the transcriptional networks controlling the adaptation of the cell to its environment. We analyzed the transcriptome response of yeast to toxic concentrations of selenite. We used gene network mapping tools to identify functional pathways and transcription factors involved in this response. We then used chromatin immunoprecipitation and knock-out experiments to investigate the role of some of these regulators and the regulatory connections between them.

Results

Selenite rapidly activates a battery of transcriptional circuits, including iron deprivation, oxidative stress and protein degradation responses. The mRNA levels of several transcriptional regulators are themselves regulated. We demonstrate the existence of a positive transcriptional loop connecting the regulator of proteasome expression, Rpn4p, to the pleiotropic drug response factor, Pdr1p. We also provide evidence for the involvement of this regulatory module in the oxidative stress response controlled by the Yap1p transcription factor and its conservation in the pathogenic yeast C. glabrata. In addition, we show that the drug resistance regulator gene YRR1 and the iron homeostasis regulator gene AFT2 are both directly regulated by Yap1p.

Conclusion

This work depicted a highly interconnected and complex transcriptional network involved in the adaptation of yeast genome expression to the presence of selenite in its chemical environment. It revealed the transcriptional regulation of PDR1 by Rpn4p, proposed a new role for the pleiotropic drug resistance network in stress response and demonstrated a direct regulatory connection between oxidative stress response and iron homeostasis.

Background

The adaptation of genome expression to the chemical environment is a complex but crucial challenge for all living cells. Functional genomics analyses in budding yeast have shown that environmental stress responses may involve rapid changes in the expression of up to 30% of the genome. A common response to all stresses, named ESR (Environmental Stress Response), has been described, which consists in the inhibition of the cytosolic translation apparatus and the activation of the energy storage pathways [1]. However, pathways responding specifically to the parameters of the environment also form a key part of the stress response. These pathways involve specific transcriptional modules that rapidly sense the environment as a series of chemical and physical features (e.g. redox, pH, osmolarity, temperature, etc.) and act together to adapt genome expression to the specific nature of each stress [2]. For instance, at least eight different transcription factors act together to define the first-hour response of yeast cells to the toxic metalloid arsenite [3]. These global and rapid responses are highly dynamic, involving sequential waves of gene activation and repression [1, 2, 4]. This requires tight temporal coordination between different transcriptional routes, which can be achieved in two complementary ways. First, the transcription factors involved in stress responses, despite responding to different signals, may have overlapping sets of targets [5]. Second, cross-regulation between transcription factors may ensure the coordinated activation of different pathways [6]. We focus here on the cross-talks between three transcriptional modules responsible for the oxidative stress response, the ubiquitine-mediated protein degradation and the pleiotropic drug resistance, respectively. These cellular pathways exist in all species, from bacteria to mammals and plants. In S. cerevisiae, the oxidative stress response is controlled principally by the Yap1p transcription factor of the AP1-like leucine zipper family. Yap1p acts as a secondary sensor for oxidative molecules, and thus responds to a wide spectra of toxic compounds, such as hydrogen peroxide, metals and metalloids, organic nucleophilic molecules and internal metabolic oxidative stress due to the production of toxic by-products during glycolysis [3, 4, 7, 8]. Yap1p recognizes YRE (Yap1p response elements, 5'-TKACTMA-3') in the promoters of genes involved in redox homeostasis and in xenobiotic export at the plasma membrane. The proteasome is involved in both the degradation of damaged or aggregated proteins and in the post-translational regulation of several biological processes, playing a key role in many stress responses [9]. Expression of the genes involved in proteasome biogenesis and activity, and in ubiquitin-dependent proteolysis, is controlled by the C2H2 zinc finger protein Rpn4p, which recognizes the PACE (proteasome associated control element, 5'-GGTGGCAAA-3') sequence in the promoter of its target genes [3, 10, 11]. Pleiotropic drug resistance involves the upregulation of membrane proteins involved in drug efflux. The corresponding genes are controlled principally by two Zn2Cys6 Gal4p-like transcription factors: Pdr1p and Pdr3p. These two transcription factors have largely overlapping sets of targets and recognize the same DNA motif (named PDRE, 5'-TCCGYGGR-3'), but have different roles and regulatory properties [2, 5, 12–14]. The Yap1p and Rpn4p pathways are simultaneously involved in the yeast response to arsenate [3]. Yap1p acts together with the Pdr1p/Pdr3p pathway to induce a drug specific response to the antifungal drug benomyl [4]. No transcriptional regulation has been described for PDR1, but YAP1, RPN4 and PDR3 are induced by stress [3, 5, 6]. In this work, we used selenite as a model stress to investigate further the interactions between these three transcriptional modules. Selenium is an essential oligoelement that replaces the sulfur atom of some methionine and cysteine in proteins involved in various essential cell functions [15]. Selenium is also a promising agent for cancer therapy and anti-aging treatments [16]. However, high doses of selenium are toxic to eukaryotic cells [17]. In yeast, selenium alters genome stability [18] and is detoxified in the vacuole after reacting with glutathione [19]. Yeast cells have a high level of selenium tolerance, and yeast enriched in selenium have been used in therapeutic trials [20]. We showed in this study that toxic doses of selenite activated various yeast stress response pathways, including the proteasome, oxidative stress, iron homeostasis and general stress pathways. We demonstrated that, in these growth conditions, the expression of PDR1 and RPN4 was coordinated through a positive transcriptional loop. This loop contributed to the optimal Yap1p-dependent oxidative stress induction of several genes encoding membrane proteins, including FLR1, ATR1 and FRM2. This function seemed to be conserved in the pathogenic yeast species C. glabrata. Finally, our data provide evidence for direct transcriptional regulation of the iron homeostasis regulator Aft2p and of the multidrug resistance regulator Yrr1p by Yap1p, indicating a broader role for this factor in coordination of the oxidative stress response.

Results

Gene ontology mapping of the selenite response

We analyzed the transcriptome of S. cerevisiae cells treated with 1 mM sodium selenite for 2, 5, 10, 20, 40, 60 and 80 minutes. The corresponding cDNAs were competitively hybridized on DNA microarrays, with cDNAs obtained from cells mock-treated for an equivalent period. Each experiment was carried out four times, with independent biological samples. We used SAM to evaluate the significance of variations in expression of each gene [21]. The dose of selenite used was the lowest dose that significantly altered cell survival in our preliminary growth assays (data not shown). This toxic dose of selenite induced large changes in the transcriptome of the cells (see additional file 1). In our data set, about 30% of the yeast ORFs displayed a significant change in expression level for at least two consecutive time points, with similar numbers of up- and downregulated. The earliest significant effects on gene expression were detected at 10 minutes, with the peak response at 40 minutes (see additional file 2 and additional file 1). We investigated the functional pathways involved in this large and complex response using the t-profiler software [22], which identified the Gene Ontology (GO) categories significantly up- or downregulated over the whole dataset (figure 1A, see additional file 3). T-profiler analysis indicated that iron homeostasis genes were among the first to be induced (figure 1A). Selenite also induced an oxidative stress response characterized by the up-regulation of genes involved in redox homeostasis and in proteasome activity (figure 1A). Finally, our selenite treatment, similarly to all stresses with deleterious effects on growth, triggered a large ESR [1]. This response was characterized by the general repression of genes involved in cytosolic translation (ribosome biogenesis, rRNA and tRNA processing, etc.) and by the induction of chaperone proteins and genes involved in energy storage and carbohydrate metabolism (figure 1A). This probabilistic model of the selenite response was confirmed by the expression profiles of genes from the GO categories cited above (figure 1B and see additional file 4). We also used T-profiler to predict the transcription factors likely to be responsible for the gene expression patterns observed, based on previous genome-wide chromatin immunoprecipitation experiments and DNA motifs in the promoters of the corresponding genes (figure 2A). This led to the identification of 14 transcription factors as possibly positively regulating gene expression in response to selenite (figure 2A, see additional file 3). This list includes regulators involved in the iron homeostasis (Aft1p, Aft2p), two regulators of the oxidative stress response (Yap1p and Skn7p) and certain Yap1p homologues (Yap7p, Cin5p and Cad1p), the regulator of proteasome expression (Rpn4p) and several regulators involved in the general stress response (Hsf1p, Msn2p, Msn4p and Gcn4p). Several transcription factors controlling stress response pathways were themselves found to be upregulated at the mRNA level following selenite treatment (figure 2B). For instance, YAP1 and RPN4 displayed a remarkable pattern of co-induction. CIN5 (YAP4), which encodes a Yap1p homologue involved in redox homeostasis and salt tolerance [23], was also induced in our experiments, together with YRR1, which encode a zinc-finger protein conferring cell resistance to drugs such as 4-NQO or reveromycine and which is upregulated by oxidizing agents such as MMS or benomyl [4, 24–26]. More surprisingly, PDR1, which encodes the major regulator of pleiotropic drug resistance in S. cerevisiae [13], and AFT2, which encodes one of the two main regulators of iron homeostasis [27, 28], were both induced by selenite, although no transcriptional regulation of these two genes has ever been reported before. We investigated possible connections between the mechanisms regulating these transcription factors.
Figure 1

Global Gene Ontology mapping of the selenite response in S. cerevisiae. (A): The gene ontology categories with levels of expression significantly changed by selenite were identified by T-profiler [22]. The graph represents only the most significant functional categories, as a function of time. More complete T-profiler results are available in additional file 3. The color code is as follows: black: E-value > 0.05 (non significant variation); red: E-value < 0.05 and t-value > 0 (positive significant variation); green: E-value < 0.05 and t-value < 0 (negative significant variation). (B): Gene expression patterns for the functional categories discussed further in the text. Wild-type cells were treated with selenite and gene expression was evaluated by microarray analyses at different time points, using untreated cells as a reference. Note that the lists of genes represented here are not exhaustive and represent a sample of the genes present in these GO categories. More complete information can be found in additional file 1.

Figure 2

Transcriptional regulation and selenite response. (A): T-profiler prediction of the transcription factors involved in the positive response of yeast to selenite. The color code is the same as for figure 1A. These predictions were based on previous ChIP-chip results [30]. (B): Expression patterns of genes encoding transcription factors involved in stress response pathways. Full results are presented in additional file 1. Wild-type cells were treated with selenite and gene expression was evaluated by microarray analyses at different time points, using untreated cells as a reference.

A transcriptional loop connects RPN4 to PDR1

We first examined the basis of the regulation of PDR1 and RPN4. The induction of these two genes by selenite was confirmed by quantitative RT-PCR (figure 3). The induction of RPN4 in response to oxidative stress was previously shown to be largely dependent on Yap1p [3, 6]. RPN4 has also been shown to be activated by gain-of-function alleles of PDR1 [12] and the RPN4 promoter contains a PDRE which is actually bound by Pdr1p in vivo and in vitro [2, 29]. This PDRE was recently shown to be essential to the full induction of RPN4 in response to oxidative stress. This effect was principally attributed to Pdr3p, which also recognizes PDRE [6]. However, pdr1Δ cells have normal basal levels of RPN4 mRNA and of proteasome activity [29] and RPN4 is not induced by drugs which efficiently trigger the Pdr1p/Pdr3p multidrug resistance response [2, 4, 5]. Finally, the impact of Pdr1p on the RPN4 expression in response to stress still had to be established. We therefore examined the expression of RPN4 in the presence of selenite in pdr1Δ cells (figure 3). We observed a slight decrease of the RPN4 induction from 60 minutes after selenite exposure. This result proved that Pdr1p has a modest but significant role in the induction of RPN4 in response to oxidative stress.
Figure 3

RPN4 and PDR1 are dependent on each other for regulation by selenite. The levels of expression of PDR1 (upper panel) and RPN4 (lower panel) after selenite or mock treatment were quantified in wild-type and rpn4Δ (upper panel) or pdr1Δ (lower panel) cells, using real-time quantitative PCR. The expression values were normalized using the gene encoding actin (ACT1, see methods). The values represented here are the ratios of the normalized expression levels of PDR1 (upper panel) or RPN4 (lower panel) in the presence of selenite, versus the normalized expression levels of these genes in mock experiments. Each measurement was repeated three times on independent samples. The standard errors are indicated.

In searches of SGD [30–32] and Yeastract [33] databases for transcriptional regulators that might account for the selenite-dependent induction of PDR1, we identified a conserved Rpn4p recognition element in the promoters of the PDR1 orthologues in Saccharomyces sensu stricto species. Moreover, Rpn4p was found to bind to the PDR1 promoter in a global study of the genomic locations of yeast transcription factors binding sites [30]. We therefore monitored PDR1 expression in an rpn4Δ strain. The Rpn4p had no apparent role in PDR1 basal expression but the inactivation of RPN4 severely reduced the sensitivity of PDR1 to selenite (figure 3). We conclude from these results that a positive transcriptional loop connects RPN4 and PDR1 in response to selenite.

The PDR1/RPN4 loop optimizes the Yap1p dependent oxidative stress response

We investigated the role of the PDR1/RPN4 loop, by carrying out a genome-wide analyses of the contributions to the selenite response of Rpn4p, Pdr1p, Yap1p, which controls RPN4 expression in response to metalloids [3], and Pdr3p, the functional homologue of Pdr1p. Fluorescent cDNAs from yap1Δ, rpn4Δ, pdr1Δ or pdr3Δ cells treated for 0, 40, 60 or 80 minutes with 1 mM of selenite, were hybridized to microarrays together with cDNA from wild type cells subjected to identical treatment (see additional file 5). We then focused on 175 genes displaying levels of induction lower than those observed for the wild type in at least one of the mutants for at least two consecutive time points (see additional file 6). These genes were classified as a function of their gene expression patterns using hierarchical clustering (see additional file 6). Based on the clustering tree obtained, we defined five groups corresponding to five different patterns of expression (figure 4A). We used Yeastract [33] and the SGD GO term finder [34] to address the main transcriptional regulators and functional annotations available for these genes (figure 4B). Yap1p was the factor regulating the largest number of these genes (about 80%), which were, as expected, mostly involved in redox homeostasis and the chemical stress response (clusters 1, 2, 4 and 5). Many of these genes had already been annotated as Yap1p targets and their selenite induction was abolished in the absence of YAP1 (clusters 1 and 2). Yap1p also had a slight effect on some genes involved in proteolysis (part of cluster 3), possibly indirectly due to the significant influence of Yap1p on RPN4 expression (see additional file 6). RPN4 deletion had an effect on about 60% of the genes shown in figure 4A. Most of these genes encoded proteins involved in proteolysis (cluster 3), as expected, and one third of them had already been identified as Rpn4p targets (figure 4B). More surprisingly, the deletion of RPN4 slightly but reproducibly decreased the selenite induction of several Yap1p targets (clusters 1 and 4). The inactivation of PDR1 or PDR3 altered the expression of few genes (less than 20% of the genes in figure 4A), all of which were Yap1p targets (upper parts of clusters 1 and 4) and encoded proteins involved in chemical stress response and xenobiotic export (FLR1, ATR1, FRM2, etc.). Remarkably, these effects were similar to those of the RPN4 deletion on these genes (figure 4C). We carried out chromatin immunoprecipitation (ChIP) analyses, together with real-time quantitative PCR, to investigate the direct binding of Pdr1p to these Pdr1p-dependent genes, using a myc-tagged version of Pdr1p [2]. We used the PDR5 promoter as a positive control, as this sequence constitutively binds Pdr1p [2]. We first focused on the FLR1 promoter, which contains a PDRE and is partially controlled by Pdr3p in response to oxidative stress [35]. We detected no significant binding of Pdr1p to the promoter of FLR1, despite using several pairs of oligonucleotides to scan the entire FLR1 promoter region (figure 5). Similarly, the promoters of ATR1, FRM2 and YCR102c did not bind by Pdr1p in the presence of selenite (data not shown). RPN4 was the only gene tested that displayed Pdr1p-dependent selenite induction, its promoter binding directly to Pdr1p (Figure 5).
Figure 4

Respective weights of RPN4 , PDR1 , PDR3 or YAP1 in the selenite response. (A) Hierarchical clustering of the genes dependent on one or several of the four transcription factors studied for selenite induction. DNA microarrays were used to compare gene expression levels between wild-type and rpn4Δ, pdr1Δ, pdr3Δ or yap1Δ exposed to selenite (times 40, 60 and 80 minutes) or mock-treated (time 0). The 175 genes displaying an alteration of selenite induction in at least one mutant strain were clustered into 5 groups (see additional files 5 and 6). (B): Schematic representation of the importance of each transcription factor in the regulation of the five clusters defined in (A). The arrows symbolize the positive regulation of each cluster by the transcription factor: the width of the arrows indicates the importance of the regulation (large arrows: strong effect, thin arrows: weak effect). Dashed arrows indicate that the transcription factor controls the expression of only some of the genes present in the cluster. Solid arrows mean that all the genes present in the cluster are regulated. The relevance of the Gene Ontology categories and regulatory relationships in each of the clusters was investigated with the SGD GO term finder and Yeastract tools [33, 34]. Only the main GO category with a p-value < 0.0001 and the main transcription factor known to regulate the genes in one cluster were indicated. (C): Enlargement of the part of cluster 1 containing the genes most sensitive to the deletion of PDR1 and PDR3. Gene names are indicated. The presence of a PDRE in the promoters of these genes is indicated by black dots.

Figure 5

Pdr1p binds to the promoter of RPN4 but not to that of FLR1 in the presence of selenite. The binding of Pdr1p to promoters was assessed by combining chromatin immunoprecipitation with real-time quantitative PCR, using strains harboring a chromosomal tagged version of Pdr1p (myc-Pdr1p). Sequence enrichment in the ChIP (i.e. ChIP/whole cell extract ratio) was normalized using the ACT1 ORF as a reference. Similar experiments were conducted on cells with the untagged Pdr1p as a negative control ("mock ChIP"). The PDR5 promoter was used as a positive control for Pdr1p binding. The results shown for FLR1 were obtained using a pair of oligonucleotides spanning the PDRE motif present in the FLR1 promoter. The cells were exposed to 1 mM of selenite (+) or mock-treated (-) for 60 minutes before the beginning of the ChIP procedure.

Conservation of the YAP1/RPN4/PDR1 co-regulation in C. glabrata

We assessed the conservation of RPN4, PDR1 and YAP1 co-regulation in the pathogenic yeast C. glabrata. This species does not belong to the Saccharomyces sensu stricto group but is much more closely related to S. cerevisiae than the other pathogenic Candida species [36]. Especially, clear C. glabrata orthologs of PDR1 (called CgPDR1), YAP1 (CgAP1) and RPN4 (CgRPN4) could be defined, whereas there seems to be no ortholog of PDR3 in this species [37]. As in S. cerevisiae, CgRPN4 is upregulated in strains harboring gain-of-function alleles of CgPDR1 [38]. We used C. glabrata microarrays to investigate the expression patterns of CgAP1, CgRPN4 and CgPDR1 in response to the oxidative stress caused by the antifungal drug, benomyl (figure 6A). A complete comparison of the oxidative stress responses of C. glabrata and S. cerevisiae is presented elsewhere (Lelandais et al., manuscript in preparation). We observed that benomyl induced the C. glabrata homologues of YAP1, RPN4 and PDR1 (figure 6A). We therefore analyzed the DNA regions upstream from the CgRPN4, CgAP1 and CgPDR1 ORFs (figure 6B). As in S. cerevisiae, two PDRE and one YRE were found in the CgRPN4 promoter, whereas one PACE was found in the CgPDR1 and CgAP1 promoters (figure 6B). These data strongly suggest that the cross-regulation of PDR1 and RPN4 on one hand, and of YAP1 and RPN4 on the other, is conserved from S. cerevisiae to C. glabrata. These findings are consistent with this network playing a role in the fine-tuning of oxidative stress responses in yeasts.
Figure 6

PDR1 , RPN4 and YAP1 are induced by oxidative stress in C. glabrata. (A) DNA microarrays were used to analyze the transcriptome response of C. glabrata cells to the oxidizing agent benomyl. The data obtained were analyzed and discussed fully in another manuscript (Lelandais et al., in preparation). (B) We searched for consensus DNA binding sites for Rpn4p, Yap1p and Pdr1p in the CgRPN4, CgAP1 and CgPDR1 promoters using RSA tools software [65]. The positions of the motifs identified are indicated relative to the start codon of the corresponding ORF. The Saccharomyces sensu stricto species used here are S. cerevisiae, S. paradoxus, S. bayanus, S. kuudriazvezii and S. mikatae. The positions of the motifs indicated for these species refer to the S. cerevisiae genes.

Expanding the Yap1 network: connections with the Yrr1 and Aft2 regulons

The YRR1 and AFT2 genes were both induced by selenite (figure 2). YRR1 was shown to be regulated by oxidizing agents such as benomyl or mancozeb in a Yap1p-dependent manner [4, 39]. No transcriptional regulation of AFT2 has ever before been reported. The selenite-driven expression of AFT2 was weaker in the yap1Δ strain than in the wild type (see additional file 6 and cluster 5 of figure 4A) and one YRE (TTAGTCA) conserved in Saccharomyces sensu stricto species, was found in the promoter of this gene 147 base pairs upstream from the ATG codon [31, 32]. We conducted genome-wide location analyses of a myc-tagged version of Yap1p [40] using the ChIP-chip technique [30]. Previous experiments indicated that Yap1p could discriminate between different sources of oxidative stress but that its DNA-binding properties were independent of the oxidizing agents used [4, 41]. We therefore increased the relevance of our ChIP-chip data, by carrying out analyses of three different sources of oxidative stress: selenite (1 mM), hydrogen peroxide (0.3 mM) and benomyl (20 μg/ml). The doses of hydrogen peroxide and benomyl and the time of exposure (20 minutes) used were chosen based on previous experiments [4, 7, 40]. Each experiment was carried out independently four times. We used the SAM method [21] to obtain lists of significantly and reproducibly bound targets in the three sets of conditions. We considered as potential targets only those sequences bound in all the oxidative conditions tested. We controlled for false positive by conducting similar experiments in control conditions, on cells treated with DMSO. In these conditions, Yap1p was efficiently excluded from the nucleus [42]. We then deleted the DNA sequences which were significantly enriched in the DMSO experiments, to obtain the final list of Yap1p direct targets presented in the additional file 7. We identified 310 DNA sequences, corresponding to about 300 promoter regions, that were reproducibly and significantly enriched in all conditions. According to the SGD GO term finder [34], the genes regulated by these promoter regions were mostly involved in the cell response to chemical stimuli (p = 2.21 × 10-18), oxidative stress (p = 1.16 × 10-9), and drugs (p = 1.35 × 10-3); and in sulfur metabolism (p = 4.1 × 10-4). This list contained many genes previously known to be regulated by Yap1p (e.g. TRX2, FRM2, TRR1, TSA2, SOD1, etc.), including CIN5 and RPN4 (see additional file 7). Remarkably, 75% of these 310 sequences contained a YRE (see additional file 7) but only 23% of them were induced by selenite in a Yap1p-dependent manner, which confirmed that Yap1p binding is necessary but not sufficient for transcriptional regulation [4, 30, 41]. Our ChIP-chip results overlapped with the clusters defined in figure 4. The groups of genes which were highly dependent on Yap1p for selenite induction (clusters 1 and 2), contained 72% and 86%, respectively, of sequences binding Yap1p in our experiments. Clusters 4 and 5 included only 50% and 33% of sequences binding Yap1p. Only 5% of the genes in cluster 3, which was mainly composed of targets of Rpn4p, were found to bind Yap1p, confirming that the mild effect of Yap1p on the expression of about half of this group is indirect. Finally, the sequences corresponding to the YRR1 and AFT2 promoters were significantly and specifically enriched in all the oxidative conditions tested (Figure 7). Based on these data, we conclude that the selenite-dependent induction of YRR1 and AFT2 is directly controlled by Yap1p.
Figure 7

Yap1p binds to the promoters of YRR1 and AFT2 in oxidative conditions. The global DNA binding pattern of Yap1p was determined by chromatin immunoprecipitation using strains harboring a tagged version of Yap1p (myc-Yap1p). The immunoprecipitate DNA was hybridized with intergenic DNA microarrays, together with genomic DNA (see methods). The cells were previously treated with benomyl, hydrogen peroxide or selenite (oxidative conditions); or with DMSO (control condition). Enrichment ratios were normalized using the print-tip median method (see methods). Each experiment was carried out four (oxidative conditions) or two (control condition) times. The histograms indicate the enrichment of the sequence in the immunoprecipitate, normalized with respect to the value obtained for whole cell extract. The results obtained for the mitochondrial ORF COX1 are shown as a negative control. The standard errors are indicated. The full results are shown in additional file 11.

Discussion

Network mapping for the selenite response in budding yeast

We used selenite as a model stress to decipher part of the transcriptional network controlling the adaptation of the genome expression to toxic environmental conditions in yeast. Our data suggested that one of the earliest effects of selenite on gene expression was the induction of an iron starvation-like response, known to be controlled by the Aft1p and Aft2p transcription factors [28, 43]. Selenite may affect iron homeostasis at two, non exclusive, levels. First, selenium can interact with iron, with a high affinity, in the culture medium [44, 45], rendering this metal unavailable to the cell. Second, it may interfere with iron homeostasis by replacing sulfur in iron-sulfur cluster protein biosynthesis in the mitochondria [46]. Interestingly, the sets of yeast mutant strains which accumulating selenium or iron are very similar, suggesting that these two elements are metabolized through similar cellular routes and affect similar cellular processes [47]. Like arsenite [3], selenite induces a strong oxidative stress response, triggering redox homeostasis pathways and proteasome activity. Selenite may affect redox homeostasis in several ways. First, each selenite molecule contains three atoms of oxygen, which may generate reactive oxygen species (ROS) during selenite reduction. Second, selenite is metabolized through interaction with thiol derivatives, including gluthatione [19], probably leading to imbalance in redox homeostasis. Third, as mentioned above, selenite may interfere with iron homeostasis and iron-sulfur cluster protein biosynthesis, potentially affecting mitochondrial activity and redox homeostasis [48]. Another feature of the oxidative stress response generated by metals and metalloids is the upregulation of genes involved in methionine and sulfur metabolism [3, 49]. This effect was not detected in our transcriptome analyses of the yeast response to selenite. This was certainly due to our experiments being carried out in rich media, in which the abundance of sulfur amino acids efficiently switched off the MET gene transcription [50], whereas the studies mentioned above were conducted in minimal media. Despite the simplicity of its molecular structure, selenite induces many different stress response pathways. Moreover, the dose of selenite used clearly compromised the cells ability to respond to stress efficiently. We were therefore able to observe the regulation of mRNA levels for many transcriptional regulators involved in stress responses. This made it possible to reveal new connections between these stress response pathways.

Transcriptional loops connect proteasome to pleiotropic drug resistance and oxidative stress response

The proteasome functions in many cellular processes, some of which are essential for cell survival as cell cycle progression or the adaptation to environmental changes [9, 51]. Its activity is therefore tightly regulated. One level of regulation is the expression of its subunits, which is controlled by the Rpn4p transcription factor in yeast [52]. RPN4 is itself positively regulated by a complex array of transcriptional controls connected to environmental stress. These include the heat shock factor Hsf1p, the multidrug resistance regulators Pdr1p and Pdr3p and the oxidative stress response major regulator, Yap1p [3, 6]. In this work, we confirmed that Pdr1p and Yap1p were required for the full selenite-driven induction of RPN4 (figure 3 and see additional file 6). Recent data have suggested that RPN4 not only is a target for stress response pathways but Rpn4p also has a direct impact on the expression of some of its regulators. Indeed, the response of YAP1 to selenite and arsenite was diminished in the absence of RPN4 (see additional file 6,[3]), as Rpn4p binds to a PACE present in the YAP1 promoter [30], providing strong evidence in favor of a transcriptional loop connecting RPN4 and YAP1. Similarly, one of the major findings of this study was that Rpn4p controls the expression of PDR1 in response to selenite (figure 3). Therefore, RPN4 establishes positive feedback loops with both the oxidative stress response and the pleiotropic drug resistance network. These loops seem to optimize part of the oxidative stress response, as the deletion of RPN4, PDR1 or PDR3 decreases the positive effect of Yap1p on some of its target genes by 50% (figure 4C). These effects on gene expression have apparently no impact on cell survival in laboratory conditions (see additional file 8), but evidence for their biological significance is provided by the observation of RPN4, YAP1 and PDR1 co-regulation in response to oxidative stress in the yeast C. glabrata (figure 6A). Our results are also consistent with those of a recent study showing that the Rpn4p, Yap1p, Pdr3p and Yrr1p transcription factors collaborate in the upregulation of FLR1 in response to oxidative stress [39]. Positive feedback loops may induce bistability in biological systems [53]. Bistability has obvious advantages in the responses of microbial cells to environmental changes. After transient exposure to stress, bistability allows some cells to maintain a particular pattern of gene expression long after the stimulus has ended. This provides a mechanism for the anticipation of future environmental changes based on past environmental conditions and can potentially generate heterogeneity in isogenic cell populations by generating bimodal population responses [53, 54]. For Rpn4p and Yap1p, the transcriptional positive feedback loop is counterbalanced by a post-translational negative feedback loop, with the levels of Rpn4p and Yap1p being negatively regulated by the proteasome [11, 55].

A third mode of functioning for the PDR pathway

Two modes of functioning of the PDR pathway have been extensively described. First, Pdr1p and Pdr3p are both involved in the basal expression and drug-dependent up-regulation of multidrug resistance transporter genes, such as PDR5, SNQ2 and YOR1 [13]. Second, Pdr3p is involved in a retrograde response negatively connecting the expression of drug resistance transporters to the mitochondrial respiratory activity [56, 57]. The tight connection described above between RPN4 and PDR1 reflects a third mode of functioning for the PDR pathway. In response to oxidative stress, Pdr1p and Pdr3p control the induction of RPN4 (figure 3, [6]), which in turn affects the expression of Yap1p target genes such as FLR1 (figure 4, [35]). Remarkably, FLR1 and RPN4 were not induced by drugs that efficiently trigger the "classic" PDR response, such as progesterone or fluphenazine [2, 5], or by mitochondrial defects that stimulate Pdr3p activity [57]. PDR1 and PDR3 also had original patterns of regulation in response to selenite. As described above, PDR1 is under the control of Rpn4p (figure 3). By contrast, PDR3 has been reported to be controlled by Hsf1p in response to oxidative stress [6]. The induction of PDR3 by selenite (see additional file 9) together with the other targets of Hsf1p (see additional file 4), suggests that the regulation of PDR3 by Hsf1p is effective in the presence of selenite (figure 8).
Figure 8

A highly interconnected transcriptional network is involved in the response to selenite. This diagram indicates the regulatory interactions involved in the response to selenite, as identified through this study. All the arrows represent positive transcriptional regulations of transcription factor encoding genes or of groups of target genes (symbolized then by functional categories) by a transcription factor. Red arrows indicate interactions demonstrated for the first time by this work. The arrows in bold indicate high-weighted interactions and the thin arrows symbolize low-weighted interactions, according to the results of this study (in the case of Rpn4p, Yap1p, Pdr1p, Pdr3p) or measurements published by Hahn et al (in the case of Hsf1p) [6].

Yap1p: a central node in the oxidative stress network

Yap1p is the major regulator of the genes encoding proteins involved in redox homeostasis in response to various sources of oxidative stress [1, 58]. Yap1p has been shown to influence other regulatory pathways, for instance by regulating RPN4 and CIN5 [3, 23]. A previous global analysis of DNA binding sites for most of the transcription factors of yeast provided the first evidence to suggest that Yap1p may regulate the expression of transcription factors [30]. We have shown that the induction of YRR1 [4] and AFT2 (figure 4) in response to stress is dependent on YAP1 and that Yap1p actually binds to the promoters of these genes in the presence of various sources of oxidative stress (figure 7). In response to both benomyl and selenite, YRR1 was induced later than other Yap1p targets (figure 2, [4]). Remarkably, several Yrr1p targets (e.g. FLR1, SNG1, SNQ2) [25] were found to be also directly regulated by Yap1p in response to selenite (see additional files 6 and 7). The role of Yrr1p therefore seems to be to support Yap1p in the long-term regulation of these genes. The physiological connection between iron metabolism and redox homeostasis has been established before. In particular, it has been shown that the activity of the Aft1p transcription factor, which senses iron through iron-sulfur protein biogenesis status [46], is influenced by the glutathione biosynthesis pathway and the Grx3 and Grx4 glutaredoxins, which are involved in the thiol redox system [48]. Moreover, the double deletion of AFT1 and AFT2 induces cell hypersensitivity to oxidative stress [27]. Our finding that Yap1p controls AFT2 expression provides a direct transcriptional connection between the two pathways. It may seem paradoxical that Yap1p positively controls a system that responds to iron starvation, given that iron uptake is likely to cause oxidative stress. Noteworthy, Aft2p specifically controls the expression of genes involved in the transport of iron from the cytosol to the vacuole and mitochondria [28]. By contrast, Aft1p, which actually controls iron uptake from the environment, does not seem to be positively regulated by Yap1p.

In conclusion, Yap1p is a central node in the oxidative stress response network, coordinating the expression of at least four transcription factors involved in various stress response pathways (figure 8).

Structure and dynamics of the regulatory networks driving cell adaptation to environmental changes

We analyzed the structure and dynamics of the transcriptional regulatory network which controls the adaptation of yeast transcriptome to toxic doses of selenite (figure 8). Our findings hinted several important features of the regulatory networks involved in chemical stress responses. First, these networks are highly interconnected. The cross-regulation of different regulators makes it possible to transmit information between the different transcriptional routes, resulting in the tight coordination of the various cellular pathways required for cell survival. Second, these networks have versatile and dynamic structures and properties. This plasticity is based on the combination of different transcription factors responsive to different chemical and physical parameters, but also on the fact that the same transcription factor can change its protein partners and/or its DNA binding properties to adapt its activity to the physiological conditions (e.g. the three modes of functioning of the Pdr1p/Pdr3p combination). Third, there is a clear hierarchy in these networks, as illustrated by unidirectional regulations (e.g. Hsf1p on RPN4) and the unequal weightings of different relationships (e.g. RPN4/YAP1 and RPN4/PDR1 loops), which may also be a function of time and physiological status of the cell.

Methods

Yeast strains

The Saccharomyces cerevisiae strains used were all of the BY4742 (MATa; his3Δ1;leu2Δ0; lysΔ0; ura3Δ0) background. The rpn4Δ, yap1Δ, pdr1Δ and pdr3Δ strains were purchased from Euroscarf [59]. The Pdr1-myc and the Yap1-myc strains have been described elsewhere [2, 40]. The Candida glabrata strain was CBS418.

Growth conditions and time-course analyses of stress responses

Cells were grown at 30°C in YPD (1%(w/v) bacto-yeast extract, 2% (w/v) bacto-peptone, 2% (v/w) glucose) to an OD600 nm of 0.5. The cultures were then split in two. Sodium selenite (1 mM), hydrogen peroxide (0.3 mM) or benomyl (20 μg/ml) was added to one of the two half-cultures and water or DMSO (mock treatment) was added to the other. The cells were incubated for an appropriate period of time (see text), and were then either flash-frozen in cold ethanol for RNA extraction or treated with formaldehyde for chromatin immunoprecipitation (see below).

RNA extractions

Cell culture (15 ml) was flash-frozen in 30 ml of absolute ethanol at -80°C. The cells were harvested by centrifugation (4 minutes at 3000 g). The cell pellets were stored at -80°C. Total RNAs was extracted as previously described [2].

Transcriptome and quantitative RT PCR analyses

The S. cerevisiae microarrays used are fully described in Array express ([60]; accession numbers A-MEXP-337, A-MEXP-114 and A-MEXP-1064). The C. glabrata microarrays are described in the Gene Expression Omnibus database ([61]; accession number: GPL3922). We used 10 μg of total RNA for cDNA synthesis and labeling. The microarray experiments were conducted as previously described [2]. Raw data were normalized using global lowess followed by print-tip median methods, with background removal, as implemented in Goulphar [62]. Experiments with wild-type strains were carried out 4 times, with dye swapping. The statistical significance of the differences in expression observed was determined with the TMEV version of SAM, with a FDR of 5% and the exact number of permutations [21, 63, 64]. Only genes with less than 25% missing values were considered for the SAM analyses. The remaining missing values were imputed by the KNN input method directly in the TMEV application [21, 63, 64]. Hierarchical clustering was performed using TMEV, with Euclidean distances and average linkage [63, 64]. The complete S. cerevisiae transcriptome data are available as additional files 1 and 5. The raw data can be downloaded from the array express database (accession number: E-TABM-439). The C. glabrata data can be downloaded from the Gene Expression Omnibus database (accession number: GSE10244).

We used 500 ng of total RNA for quantitative RT-PCR, which was performed as previously described [2]. The oligonucleotides used are described in additional file 10. ACT1 was used as a reference for normalization.

Chromatin immunoprecipitation

Chromatin immunoprecipitation experiments, followed by intergenic microarray or quantitative PCR analyses, were performed as previously described [2]. The S. cerevisiae intergenic arrays are described in the Array express database (accession number: A-MEXP-1065). The array results were normalized using the print-tip median [62]. The statistical significance of the ChIP enrichments was assessed with the TMEV version of SAM with a FDR of 1% and the exact number of permutations [21, 63, 64]. The complete ChIP-chip results are available as additional file 11. The raw data can be downloaded from the array express database (accession number: E-TABM-437). The sequence of the oligonucleotides used for quantitative PCR can be found in additional file 10.

Data mining

Functional analyses and network mapping of the genome-wide data were carried out with T-profiler [22], SGD GO term finder [32] or Yeastract [33], using the default parameters. Promoter sequence analyses were performed with the DNA pattern search tool from RSA tools [65] and the genome Browser tool from the SGD [66].

Notes

Abbreviations

ESR: 

environmental stress response

YRE: 

Yap1p response element

PACE: 

proteasome associated control element

PDRE: 

pleiotropic drug response element

ORF: 

open reading frame

GO: 

gene ontology

MMS: 

methyl methane sulfonate

4-NQO: 

nitroquinoline oxide

DMSO: 

dimethyl sulfoxyde

SGD: 

saccharomyces genome database

ChIP: 

chromatine immunoprecipitation

ChIP-chip: 

ChIP associated to microarrays

SAM: 

significance analysis of microarrays

ROS: 

reactive oxygen species

MET: 

methionine biosynthesis pathway

PDR: 

pleiotropic drug resistance.

Declarations

Acknowledgements

VF was the recipient of a fellowship from the French ministery of research (MESR). HS had a post-doctoral grant from the "Toxicologie nucléaire" French national program. EP was funded by the Italian Istituto Pasteur-Fondazione Cenci bolognetti. We are grateful to the Service de Génomique Fonctionnelle, CEA-Evry, and in particular to Frank Amyot and Xavier Gidrol, who kindly provided the yeast intergenic microarrays. This work was supported by the "Toxicologie nucléaire" program.

Authors’ Affiliations

(1)
Laboratoire de génétique moléculaire
(2)
Plate-forme transcriptome
(3)
Muséum national d'Histoire naturelle
(4)
Commissariat à l'Energie Atomique, Institut de Biologie et de Technologies de Saclay
(5)
Equipe de Bioinformatique Génomique et Moléculaire, INSERM UMR S726, Université Paris 7

References

  1. Gasch AP, Spellman PT, Kao CM, Carmel-Harel O, Eisen MB, Storz G, Botstein D, Brown PO: Genomic expression programs in the response of yeast cells to environmental changes. Mol Biol Cell. 2000, 11: 4241-4257.PubMed CentralView ArticleGoogle Scholar
  2. Fardeau V, Lelandais G, Oldfield A, Salin H, Lemoine S, Garcia M, Tanty V, Le Crom S, Jacq C, Devaux F: The central role of PDR1 in the foundation of yeast drug resistance. J Biol Chem. 2007, 282: 5063-5074.View ArticleGoogle Scholar
  3. Haugen AC, Kelley R, Collins JB, Tucker CJ, Deng C, Afshari CA, Brown JM, Ideker T, Van Houten B: Integrating phenotypic and expression profiles to map arsenic-response networks. Genome Biol. 2004, 5: R95-PubMed CentralView ArticleGoogle Scholar
  4. Lucau-Danila A, Lelandais G, Kozovska Z, Tanty V, Delaveau T, Devaux F, Jacq C: Early expression of yeast genes affected by chemical stress. Mol Cell Biol. 2005, 25: 1860-1868.PubMed CentralView ArticleGoogle Scholar
  5. Banerjee D, Lelandais G, Shukla S, Mukhopadhyay G, Jacq C, Devaux F, Prasad R: The steroid responses of pathogenic and non-pathogenic yeast species enlighten the functioning and evolution of multidrug resistance transcriptional networks. Eukaryot Cell. 2007Google Scholar
  6. Hahn JS, Neef DW, Thiele DJ: A stress regulatory network for co-ordinated activation of proteasome expression mediated by yeast heat shock transcription factor. Mol Microbiol. 2006, 60: 240-251.View ArticleGoogle Scholar
  7. Delaunay A, Pflieger D, Barrault MB, Vinh J, Toledano MB: A thiol peroxidase is an H2O2 receptor and redox-transducer in gene activation. Cell. 2002, 111: 471-481.View ArticleGoogle Scholar
  8. Maeta K, Izawa S, Okazaki S, Kuge S, Inoue Y: Activity of the Yap1 transcription factor in Saccharomyces cerevisiae is modulated by methylglyoxal, a metabolite derived from glycolysis. Mol Cell Biol. 2004, 24: 8753-8764.PubMed CentralView ArticleGoogle Scholar
  9. Wolf DH, Hilt W: The proteasome: a proteolytic nanomachine of cell regulation and waste disposal. Biochim Biophys Acta. 2004, 1695: 19-31.View ArticleGoogle Scholar
  10. Mannhaupt G, Schnall R, Karpov V, Vetter I, Feldmann H: Rpn4p acts as a transcription factor by binding to PACE, a nonamer box found upstream of 26S proteasomal and other genes in yeast. FEBS Lett. 1999, 450: 27-34.View ArticleGoogle Scholar
  11. Xie Y, Varshavsky A: RPN4 is a ligand, substrate, and transcriptional regulator of the 26S proteasome: a negative feedback circuit. Proc Natl Acad Sci U S A. 2001, 98: 3056-3061.PubMed CentralView ArticleGoogle Scholar
  12. Devaux F, Marc P, Bouchoux C, Delaveau T, Hikkel I, Potier MC, Jacq C: An artificial transcription activator mimics the genome-wide properties of the yeast Pdr1 transcription factor. EMBO Rep. 2001, 2: 493-498.PubMed CentralView ArticleGoogle Scholar
  13. Moye-Rowley WS: Transcriptional control of multidrug resistance in the yeast Saccharomyces. Prog Nucleic Acid Res Mol Biol. 2003, 73: 251-279.View ArticleGoogle Scholar
  14. DeRisi J, van den Hazel B, Marc P, Balzi E, Brown P, Jacq C, Goffeau A: Genome microarray analysis of transcriptional activation in multidrug resistance yeast mutants. FEBS Lett. 2000, 470: 156-160.View ArticleGoogle Scholar
  15. Papp LV, Lu J, Holmgren A, Khanna KK: From selenium to selenoproteins: synthesis, identity, and their role in human health. Antioxid Redox Signal. 2007, 9: 775-806.View ArticleGoogle Scholar
  16. Patrick L: Selenium biochemistry and cancer: a review of the literature. Altern Med Rev. 2004, 9: 239-258.Google Scholar
  17. Letavayova L, Vlckova V, Brozmanova J: Selenium: from cancer prevention to DNA damage. Toxicology. 2006, 227: 1-14.View ArticleGoogle Scholar
  18. Letavayova L, Vlasakova D, Spallholz JE, Brozmanova J, Chovanec M: Toxicity and mutagenicity of selenium compounds in Saccharomyces cerevisiae. Mutat Res. 2008, 638: 1-10.View ArticleGoogle Scholar
  19. Tarze A, Dauplais M, Grigoras I, Lazard M, Ha-Duong NT, Barbier F, Blanquet S, Plateau P: Extracellular production of hydrogen selenide accounts for thiol-assisted toxicity of selenite against Saccharomyces cerevisiae. J Biol Chem. 2007, 282: 8759-8767.View ArticleGoogle Scholar
  20. Whanger PD: Selenium and its relationship to cancer: an update. Br J Nutr. 2004, 91: 11-28.View ArticleGoogle Scholar
  21. Tusher VG, Tibshirani R, Chu G: Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci U S A. 2001, 98: 5116-5121.PubMed CentralView ArticleGoogle Scholar
  22. Boorsma A, Foat BC, Vis D, Klis F, Bussemaker HJ: T-profiler: scoring the activity of predefined groups of genes using gene expression data. Nucleic Acids Res. 2005, 33: W592-5.PubMed CentralView ArticleGoogle Scholar
  23. Nevitt T, Pereira J, Rodrigues-Pousada C: YAP4 gene expression is induced in response to several forms of stress in Saccharomyces cerevisiae. Yeast. 2004, 21: 1365-1374.View ArticleGoogle Scholar
  24. Cui Z, Shiraki T, Hirata D, Miyakawa T: Yeast gene YRR1, which is required for resistance to 4-nitroquinoline N-oxide, mediates transcriptional activation of the multidrug resistance transporter gene SNQ2. Mol Microbiol. 1998, 29: 1307-1315.View ArticleGoogle Scholar
  25. Le Crom S, Devaux F, Marc P, Zhang X, Moye-Rowley WS, Jacq C: New insights into the pleiotropic drug resistance network from genome-wide characterization of the YRR1 transcription factor regulation system. Mol Cell Biol. 2002, 22: 2642-2649.PubMed CentralView ArticleGoogle Scholar
  26. Jelinsky SA, Samson LD: Global response of Saccharomyces cerevisiae to an alkylating agent. Proc Natl Acad Sci U S A. 1999, 96: 1486-1491.PubMed CentralView ArticleGoogle Scholar
  27. Blaiseau PL, Lesuisse E, Camadro JM: Aft2p, a novel iron-regulated transcription activator that modulates, with Aft1p, intracellular iron use and resistance to oxidative stress in yeast. J Biol Chem. 2001, 276: 34221-34226.View ArticleGoogle Scholar
  28. Courel M, Lallet S, Camadro JM, Blaiseau PL: Direct activation of genes involved in intracellular iron use by the yeast iron-responsive transcription factor Aft2 without its paralog Aft1. Mol Cell Biol. 2005, 25: 6760-6771.PubMed CentralView ArticleGoogle Scholar
  29. Owsianik G, Balzi l L, Ghislain M: Control of 26S proteasome expression by transcription factors regulating multidrug resistance in Saccharomyces cerevisiae. Mol Microbiol. 2002, 43: 1295-1308.View ArticleGoogle Scholar
  30. Harbison CT, Gordon DB, Lee TI, Rinaldi NJ, Macisaac KD, Danford TW, Hannett NM, Tagne JB, Reynolds DB, Yoo J, Jennings EG, Zeitlinger J, Pokholok DK, Kellis M, Rolfe PA, Takusagawa KT, Lander ES, Gifford DK, Fraenkel E, Young RA: Transcriptional regulatory code of a eukaryotic genome. Nature. 2004, 431: 99-104.PubMed CentralView ArticleGoogle Scholar
  31. Kellis M, Patterson N, Endrizzi M, Birren B, Lander ES: Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature. 2003, 423: 241-254.View ArticleGoogle Scholar
  32. Christie KR, Weng S, Balakrishnan R, Costanzo MC, Dolinski K, Dwight SS, Engel SR, Feierbach B, Fisk DG, Hirschman JE, Hong EL, Issel-Tarver L, Nash R, Sethuraman A, Starr B, Theesfeld CL, Andrada R, Binkley G, Dong Q, Lane C, Schroeder M, Botstein D, Cherry JM: Saccharomyces Genome Database (SGD) provides tools to identify and analyze sequences from Saccharomyces cerevisiae and related sequences from other organisms. Nucleic Acids Res. 2004, 32: D311-4.PubMed CentralView ArticleGoogle Scholar
  33. Teixeira MC, Monteiro P, Jain P, Tenreiro S, Fernandes AR, Mira NP, Alenquer M, Freitas AT, Oliveira AL, Sa-Correia I: The YEASTRACT database: a tool for the analysis of transcription regulatory associations in Saccharomyces cerevisiae. Nucleic Acids Res. 2006, 34: D446-51.PubMed CentralView ArticleGoogle Scholar
  34. Boyle EI, Weng S, Gollub J, Jin H, Botstein D, Cherry JM, Sherlock G: GO::TermFinder--open source software for accessing Gene Ontology information and finding significantly enriched Gene Ontology terms associated with a list of genes. Bioinformatics. 2004, 20: 3710-3715.PubMed CentralView ArticleGoogle Scholar
  35. Broco N, Tenreiro S, Viegas CA, Sa-Correia I: FLR1 gene (ORF YBR008c) is required for benomyl and methotrexate resistance in Saccharomyces cerevisiae and its benomyl-induced expression is dependent on pdr3 transcriptional regulator. Yeast. 1999, 15: 1595-1608.View ArticleGoogle Scholar
  36. Dujon B, Sherman D, Fischer G, Durrens P, Casaregola S, Lafontaine I, De Montigny J, Marck C, Neuveglise C, Talla E, Goffard N, Frangeul L, Aigle M, Anthouard V, Babour A, Barbe V, Barnay S, Blanchin S, Beckerich JM, Beyne E, Bleykasten C, Boisrame A, Boyer J, Cattolico L, Confanioleri F, De Daruvar A, Despons L, Fabre E, Fairhead C, Ferry-Dumazet H, Groppi A, Hantraye F, Hennequin C, Jauniaux N, Joyet P, Kachouri R, Kerrest A, Koszul R, Lemaire M, Lesur I, Ma L, Muller H, Nicaud JM, Nikolski M, Oztas S, Ozier-Kalogeropoulos O, Pellenz S, Potier S, Richard GF, Straub ML, Suleau A, Swennen D, Tekaia F, Wesolowski-Louvel M, Westhof E, Wirth B, Zeniou-Meyer M, Zivanovic I, Bolotin-Fukuhara M, Thierry A, Bouchier C, Caudron B, Scarpelli C, Gaillardin C, Weissenbach J, Wincker P, Souciet JL: Genome evolution in yeasts. Nature. 2004, 430: 35-44.View ArticleGoogle Scholar
  37. Sherman D, Durrens P, Iragne F, Beyne E, Nikolski M, Souciet JL: Genolevures complete genomes provide data and tools for comparative genomics of hemiascomycetous yeasts. Nucleic Acids Res. 2006, 34: D432-5.PubMed CentralView ArticleGoogle Scholar
  38. Vermitsky JP, Earhart KD, Smith WL, Homayouni R, Edlind TD, Rogers PD: Pdr1 regulates multidrug resistance in Candida glabrata: gene disruption and genome-wide expression studies. Mol Microbiol. 2006, 61: 704-722.View ArticleGoogle Scholar
  39. Teixeira MC, Dias PJ, Simoes T, Sa-Correia I: Yeast adaptation to mancozeb involves the up-regulation of FLR1 under the coordinate control of Yap1, Rpn4, Pdr3, and Yrr1. Biochem Biophys Res Commun. 2008, 367: 249-255.View ArticleGoogle Scholar
  40. Delaunay A, Isnard AD, Toledano MB: H2O2 sensing through oxidation of the Yap1 transcription factor. Embo J. 2000, 19: 5157-5166.PubMed CentralView ArticleGoogle Scholar
  41. Azevedo D, Tacnet F, Delaunay A, Rodrigues-Pousada C, Toledano MB: Two redox centers within Yap1 for H2O2 and thiol-reactive chemicals signaling. Free Radic Biol Med. 2003, 35: 889-900.View ArticleGoogle Scholar
  42. Kuge S, Jones N, Nomoto A: Regulation of yAP-1 nuclear localization in response to oxidative stress. Embo J. 1997, 16: 1710-1720.PubMed CentralView ArticleGoogle Scholar
  43. Shakoury-Elizeh M, Tiedeman J, Rashford J, Ferea T, Demeter J, Garcia E, Rolfes R, Brown PO, Botstein D, Philpott CC: Transcriptional remodeling in response to iron deprivation in Saccharomyces cerevisiae. Mol Biol Cell. 2004, 15: 1233-1243.PubMed CentralView ArticleGoogle Scholar
  44. Su C, Suarez DL: Selenate and selenite sorption on iron oxides: an infrared and electrophoretic study. Soil Sci Soc Am J. 2000, 64: 101-111.View ArticleGoogle Scholar
  45. Seby F, Potin-Gautier M, Giffaut E, Donard OFX: Assessing the speciation and the biogeochemical processes affecting the mobility of selenium from a geological repository of radioactive wastes to the biosphere. Analysis. 1998, 26: 193-198.View ArticleGoogle Scholar
  46. Rutherford JC, Ojeda L, Balk J, Muhlenhoff U, Lill R, Winge DR: Activation of the iron regulon by the yeast Aft1/Aft2 transcription factors depends on mitochondrial but not cytosolic iron-sulfur protein biogenesis. J Biol Chem. 2005, 280: 10135-10140.View ArticleGoogle Scholar
  47. Eide DJ, Clark S, Nair TM, Gehl M, Gribskov M, Guerinot ML, Harper JF: Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae. Genome Biol. 2005, 6: R77-PubMed CentralView ArticleGoogle Scholar
  48. Toledano MB, Kumar C, Le Moan N, Spector D, Tacnet F: The system biology of thiol redox system in Escherichia coli and yeast: differential functions in oxidative stress, iron metabolism and DNA synthesis. FEBS Lett. 2007, 581: 3598-3607.View ArticleGoogle Scholar
  49. Fauchon M, Lagniel G, Aude JC, Lombardia L, Soularue P, Petat C, Marguerie G, Sentenac A, Werner M, Labarre J: Sulfur sparing in the yeast proteome in response to sulfur demand. Mol Cell. 2002, 9: 713-723.View ArticleGoogle Scholar
  50. Kuras L, Rouillon A, Lee T, Barbey R, Tyers M, Thomas D: Dual regulation of the met4 transcription factor by ubiquitin-dependent degradation and inhibition of promoter recruitment. Mol Cell. 2002, 10: 69-80.View ArticleGoogle Scholar
  51. Auld KL, Silver PA: Transcriptional regulation by the proteasome as a mechanism for cellular protein homeostasis. Cell Cycle. 2006, 5: 1503-1505.View ArticleGoogle Scholar
  52. Hanna J, Finley D: A proteasome for all occasions. FEBS Lett. 2007, 581: 2854-2861.PubMed CentralView ArticleGoogle Scholar
  53. Ingolia NT, Murray AW: Positive-feedback loops as a flexible biological module. Curr Biol. 2007, 17: 668-677.PubMed CentralView ArticleGoogle Scholar
  54. Zordan RE, Miller MG, Galgoczy DJ, Tuch BB, Johnson AD: Interlocking transcriptional feedback loops control white-opaque switching in Candida albicans. PLoS Biol. 2007, 5: e256-PubMed CentralView ArticleGoogle Scholar
  55. Yokoyama H, Mizunuma M, Okamoto M, Yamamoto J, Hirata D, Miyakawa T: Involvement of calcineurin-dependent degradation of Yap1p in Ca2+-induced G2 cell-cycle regulation in Saccharomyces cerevisiae. EMBO Rep. 2006, 7: 519-524.PubMed CentralGoogle Scholar
  56. Moye-Rowley WS: Retrograde regulation of multidrug resistance in Saccharomyces cerevisiae. Gene. 2005, 354: 15-21.View ArticleGoogle Scholar
  57. Devaux F, Carvajal E, Moye-Rowley S, Jacq C: Genome-wide studies on the nuclear PDR3-controlled response to mitochondrial dysfunction in yeast. FEBS Lett. 2002, 515: 25-28.View ArticleGoogle Scholar
  58. Lee J, Godon C, Lagniel G, Spector D, Garin J, Labarre J, Toledano MB: Yap1 and Skn7 control two specialized oxidative stress response regulons in yeast. J Biol Chem. 1999, 274: 16040-16046.View ArticleGoogle Scholar
  59. EUROSCARF. [http://web.uni-frankfurt.de/fb15/mikro/euroscarf/]
  60. ArrayExpress. [http://www.ebi.ac.uk/microarray-as/aer/entry]
  61. GEO. [http://www.ncbi.nlm.nih.gov/geo/]
  62. Lemoine S, Combes F, Servant N, Le Crom S: Goulphar: rapid access and expertise for standard two-color microarray normalization methods. BMC Bioinformatics. 2006, 7: 467-PubMed CentralView ArticleGoogle Scholar
  63. Saeed AI, Bhagabati NK, Braisted JC, Liang W, Sharov V, Howe EA, Li J, Thiagarajan M, White JA, Quackenbush J: TM4 microarray software suite. Methods Enzymol. 2006, 411: 134-193.View ArticleGoogle Scholar
  64. Saeed AI, Sharov V, White J, Li J, Liang W, Bhagabati N, Braisted J, Klapa M, Currier T, Thiagarajan M, Sturn A, Snuffin M, Rezantsev A, Popov D, Ryltsov A, Kostukovich E, Borisovsky I, Liu Z, Vinsavich A, Trush V, Quackenbush J: TM4: a free, open-source system for microarray data management and analysis. Biotechniques. 2003, 34: 374-378.Google Scholar
  65. van Helden J: Regulatory sequence analysis tools. Nucleic Acids Res. 2003, 31: 3593-3596.PubMed CentralView ArticleGoogle Scholar
  66. Stein LD, Mungall C, Shu S, Caudy M, Mangone M, Day A, Nickerson E, Stajich JE, Harris TW, Arva A, Lewis S: The generic genome browser: a building block for a model organism system database. Genome Res. 2002, 12: 1599-1610.PubMed CentralView ArticleGoogle Scholar

Copyright

© Salin et al; licensee BioMed Central Ltd. 2008

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Advertisement