Bioinformatic prediction of transcription factor binding sites at promoter regions of genes for photoperiod and vernalization responses in model and temperate cereal plants
© The Author(s). 2016
Received: 20 April 2016
Accepted: 7 July 2016
Published: 8 August 2016
Many genes involved in responses to photoperiod and vernalization have been characterized or predicted in Arabidopsis (Arabidopsis thaliana), Brachypodium (Brachypodium distachyon), wheat (Triticum aestivum) and barley (Hordeum vulgare). However, little is known about the transcription regulation of these genes, especially in the large, complex genomes of wheat and barley.
We identified 68, 60, 195 and 61 genes that are known or postulated to control pathways of photoperiod (PH), vernalization (VE) and pathway integration (PI) in Arabidopsis, Brachypodium, wheat and barley for predicting transcription factor binding sites (TFBSs) in the promoters of these genes using the FIMO motif search tool of the MEME Suite. The initial predicted TFBSs were filtered to confirm the final numbers of predicted TFBSs to be 1066, 1379, 1528, and 789 in Arabidopsis, Brachypodium, wheat and barley, respectively. These TFBSs were mapped onto the PH, VE and PI pathways to infer about the regulation of gene expression in Arabidopsis and cereal species. The GC contents in promoters, untranslated regions (UTRs), coding sequences and introns were higher in the three cereal species than those in Arabidopsis. The predicted TFBSs were most abundant for two transcription factor (TF) families: MADS-box and CSD (cold shock domain). The analysis of publicly available gene expression data showed that genes with similar numbers of MADS-box and CSD TFBSs exhibited similar expression patterns across several different tissues and developmental stages. The intra-specific Tajima D-statistics of TFBS motif diversity showed different binding specificity among different TF families. The inter-specific Tajima D-statistics suggested faster TFBS divergence in TFBSs than in coding sequences and introns. Mapping TFBSs onto the PH, VE and PI pathways showed the predominance of MADS-box and CSD TFBSs in most genes of the four species, and the difference in the pathway regulations between Arabidopsis and the three cereal species.
Our approach to associating the key flowering genes with their potential TFs through prediction of putative TFBSs provides a framework to explore regulatory mechanisms of photoperiod and vernalization responses in flowering plants. The predicted TFBSs in the promoters of the flowering genes provide a basis for molecular characterization of transcription regulation in the large, complex genomes of important crop species, wheat and barley.
The genetic basis of flowering time control has been studied extensively in the model plant Arabidopsis (Arabidopsis thaliana) with over 200 putative flowering-related genes being identified [1, 2]. These genes have served as the reference for genome-wide prediction of flowering gene homologs in other plants including a cereal model species Brachypodium (Brachypodium distachyon), and two important cereal crops in short-season cropping regions, wheat (Triticum aestivum) and barley (Hordeum vulgare) [3, 4]. Such an approach assumes evolutionary conservation of flowering genes between Arabidopsis and other plants, but, different flowering pathways show varying degrees of evolutionary conservation between Arabidopsis and cereals [5–9]. The photoperiod pathway, particularly the circadian clock entrainment, is relatively conserved between Arabidopsis and monocot species . For example, a recent study reported that two-thirds of the key circadian clock components are conserved in Arabidopsis and barley , including critical photoperiod genes like CONSTANS (CO), EARLY FLOWERING 4 (ELF4), and PSEUDO RESPONSE REGULATORs (PRRs). In contrast, another major flowering-related pathway controlling the vernalization response pathway is reported to be less conserved between Arabidopsis and monocots [5–9].
Like in all other regulated genes, the expression of flowering genes is regulated by regions of non-coding DNA known as cis-regulatory elements (CREs) that contain transcription factor binding sites (TFBSs) to regulate the gene transcription. The two most well-characterized types of CREs are promoters and enhancers . A promoter is about 100–1000 bp long and it is often located at upstream of a transcription start site (TSS) of a regulated gene. A specific DNA sequence in the promoter provides a secure initial binding site for RNA polymerase and for other transcription factors (TFs) that recruit RNA polymerase. Thus the positions and sequences of promoters can be inferred with relative ease from their immediate physical proximity to the regulated genes. On the other hand, while an enhancer is also a short (50–1500 bp) region of DNA that can be bound by TFs (i.e., activators) for transcription regulation of a gene, it is located up to 1 Mbp away from the regulated gene in either upstream or downstream from the TSSin the forward or backward direction. Thus there is currently no single ‘enhancer marker’ for genome-wide identification of enhancers because all conservation- or epigenomics-based predictions show that some enhancer regions are missed (false negatives), and other sequences predicted to be active enhancers cannot be validated by complementary methods (false positives).
Some empirical studies have revealed the effects of sequence variations surrounding TFBSs at promoter regions of the flowering-related genes on the photoperiod sensitivity and vernalization requirement in cereal species. A 2,089 bp deletion in the upstream of the wheat PHOTOPERIOD1 (PPD1) gene (Ppd-A1 and Ppd-D1) can reduce the photoperiod sensitivity, resulting in early heading time [13–15]. Similarly, an insertion of 308 bp and a deletion of 1,085 bp in the upstream region of Ppd-A1 were shown to accelerate heading by 7 – 9 days compared with the photoperiod-sensitive genotype at this gene locus . This photoperiod insensitive phenotype was caused by the removal of one or more regulatory regions, which are involved in TF binding and regulation of PPD1 [14, 16]. Three independent deletions in the promter regions of VERNALIZATION1 (VRN1) in Triticum monococcum caused elevated gene expression and reduced vernalization requirement . Coversely, single-nucleotide polymorphisms (SNPs) in the promoter of VRN1-D and intron deletion resulted in its reduced expression and increased vernalization requirement in wheat . Furthermore, insertion/deletion in the first intron of VRN1 in wheat and barley can also reduce their vernalization requirement [19–21]. Recently, Kippes et al.  showed that three adajcent SNPs in a regulatory region of the wheat VRN-D4 first intron disrupt the binding of GLYCINE-RICH RNA-BINDING PROTEIN 2 (TaGRP2), a known repressor of VRN1 expression. The effect of intron deletion is intriguing because introns have been historically considered as junk DNA, though recent work shows that the first intron can also habour regulatory signals [22, 23]. Further sequence analysis showed that variations causing changes in photoperiod sensitivity and vernalization requirement in wheat and barley were not detected in the protein-coding regions of PPD1 or VRN1 [16, 17]. Therefore, TFBS identification, like gene annotation, is also essential to elucidate the molecular basis of flowering transition in plant species.
Experimental techniques are available to identify TFBSs in the promoter regions of target genes, including CHIP-Seq (chromatin immunoprecipitation coupled with massively parallel DNA sequencing) and protein binding microarray (PBM) [24–26]. However, these techniques have limitations such as the GC-content bias and high cost, and require a considerable amount of downstream data processing. Thus, bioinformatic approaches have also been developed for TFBS prediction and they typically depend on position weight matrices (PWMs) corresponding to TFs as the scoring matrices [27–29]. Most PWMs are derived from binding motifs that are determined experimentally for a given TF, and they can be obtained in public databases such as JASPAR, TRANSFAC, and CIS-BP [24, 30, 31]. Additionally, approaches using GC content as DNA free energy profiles to predict TFBSs in plants have also been developed [32, 33].
To date, the TFBS annotation in plant regulatory sequences is still largely limited, with most work being done in model plant Arabidopsis, such as AGRIS . The objective of this study was to predict distributions and related properties of TFBSs in the promoter regions of flowering-related genes in the Arabidopsis genome and the genomes of three cereal species, Brachypodium, wheat and barley. Model cereal plant Brachypodium is included as an effort to bridge the knowledge gap between the well-characterized Arabidopsis genome and the large, complex wheat/barley genomes. Despite the potential significance of enhancers and other CREs in controlling the level of transcription of flowering genes in response to environmental stimuli, this study focused on predicting TFBSs in the promoter regions of the genes only. Our approach consisted of the following steps. First, we identified the genes involved in the pathways of photoperiod (PH) and vernalization (VE), and the pathway integration (PI) genes that control the convergence point of the PH and VE pathways [6, 9, 35] in Arabidopsis, Brachypodium, wheat and barley. Second, we predicted TFBSs in the promoter regions of these genes and assessed the divergence of the TFBSs relative to their coding sequences (CDS) and introns of the orthologous genes. Third, we analyzed the public microarray data sets to assess the relationship between putative TFBSs and gene expression profiles. Finally, we mapped putative TFBSs onto the genes of the PH, VE and PI pathways in the four species, with the three genomes (A, B, and D) of allohexaploid wheat being treated separately.
Identification of genes in photoperiod, vernalization and pathway integration
In this study, we only focused on genes whose transcription regulation is related to the PH, VE and PI pathways. The lists of genes in Arabidopsis, wheat and barley were taken from our previous work , and their orthologous genes in Brachypodium were added, largely following Higgins et al. . Because some gene identifiers reported in Higgins et al.  were no longer present in the latest release of the Brachypodium genome used in this analysis, we mapped their old IDs to the new IDs using their protein sequences with BLASTP . If known genes in wheat and barley were available in GenBank, their corresponding genes in EnsemblPlants were identified using their protein sequences and BLASTP .
The promoter sequences of the PH, VE and PI genes in Arabidopsis, Brachypodium, wheat and barley were retrieved from the RSAT Plants server , using transcript as position reference. Here, we defined a promoter region as a stretch of up to 1000 bp upstream from the TSS of each gene. If another adjacent gene is located within less than 1000 bp upstream of the study gene, we only retrieved the longest possible promoter sequence to avoid any overlap with the upstream gene. The promoter sequences were examined to exclude those with more than 90 % N’s (representing gaps) in wheat and barley from further analyses. The coding and intron sequences of the genes were retrieved using the Ensembl Plants database , via its Perl API (application program interface).
Prediction of transcription factor binding sites (TFBSs) in the promoter regions
To predict TFBSs in the upstream promoter regions, we installed a standalone version of the MEME suite, which includes the FIMO motif search tool [28, 39]. For this FIMO motif discovery, we first collected a non-redundant set of position-weight matrices (PWMs) for binding profiles of known TFs. Briefly, we downloaded 64 PWMs of plant transcription factors from JASPAR (http://jaspar.genereg.net) . Then we combined a total of 725 PWMs of five species in CIS-BP (http://cisbp.ccbr.utoronto.ca) : Arabidopsis (309), Brachypodium (192), maize (Zea mays; 209), wheat (9) and barley (6). The PWMs of another cereal crop maize were included as well because it has more than 200 PWMs. As these PWMs were from different databases and species, duplicated or very similar PWMs for a given TF were occasionally found. These redundant PWMs were removed through comparing (i) the motif ID with its corresponding TF name on the motif definition line, (ii) the matrices themselves. For example, only one PWM was retained among those with over 80 % similarity (including their reverse complement matrices), because a TF can generally tolerate a limited number of substitutions within its binding site . We implemented this cleaning process in R . Additionally, among duplicated or very similar PWMs, we retained the ones derived from experimental data. After these filtering steps, the resulting set of 371 unique PWM models were used for TFBS prediction, corresponding to 345 cognate TFs.
For the FIMO background model, we retrieved the promoter sequences of all annotated genes for each species (excluding the genes in this study) from RSAT, and generated 0-order Markov-chain frequencies of the four nucleotides for each species, with the fasta-get-Markov tool in the MEME suite (Additional file 1: Table S1). For other main options of FIMO, we searched TFBSs on either strand of the promoter sequences, and set Q-value threshold as 0.2 and ‘motif-pseudo’ as 10-8. The Q-value cutoff was set as suggested by Storey , to control false discovery rate (FDR). The value of the motif-pseudo parameter was added to avoid zero probability on any position in a matrix, and we found the FIMO default of 0.1 is too large for PWMs, as some positions in many PWMs contain values of far smaller than 0.1 (Data not shown). The FIMO default of 0.1 might be more appropriate for position frequency matrices or PFMs instead of PWMs. Further cleaning of the FIMO output was carried out as well. For putative binding sites of the same TF family predicted on overlapping promoter sequence regions (or start/end positions covered by a long TFBS of the same TF family), we only retained the longest one (which often covers several short motifs) or the one with the smallest P-value calculated by FIMO.
The TF information on each predicted TFBS was added using the TF-motif association files from CIS-BP for the following species: Arabidopsis, Brachypodium, maize, wheat, barley, Antirrhinum majus, Petunia x hybrida, and Pisum sativum. It should be noted that the PWMs were derived from all the eight species listed, not just the four species used for our study.
Gene expression analysis
The Affymetrix microarray data in Arabidopsis, Brachypodium, wheat and barley were taken from the Plant Expression Database PLEXdb, with their experiment accession identifications being AT40, BD1, TA3 and BB3, respectively. However, the data set form Brachypodium (BD1) is a time-series (0 to 48 h) assay under four diurnal/circadian treatments: LLHH (Light day, Light night, Hot day, Hot night), LDHH (Light day, Dark night, Hot day, Hot night), LDHC (Light day, Dark night, Hot day, Cold night), and LLHC (Light day, Light night, Hot day, Cold night) (Mockler T. unpublished data).
From these microarray data sets, we only considered the tissues or developmental stages of high relavence to flowering, such as leaf and floral organs, but not those of little relavence to flowering such as root, shoot and seed (embryo and endosperm). The raw data files (.CEL) were normalized and transformed into log2-based expression values in a consistent procedure using Bioconductor packages in the R statistical environment [41, 43]. Heat maps of expression data were generated with the heatmap.2 function in the R gplots package.
It should be noted that RNA-seq data would be preferred given its advantages over the microarray data including unbiased detection of novel transcripts and increased specificity and sensitivity of detecting differential expression . However, it was difficult to find RNA-seq data from the same or similar plant tissues of all four species for valid and reliable cross-species comparison. Therefore, our assessment of gene expression profiles was based on the microarray data that were publicably available for the similar plant issues of all four species.
Statistical analysis of TFBS divergence
For this analysis, we first clustered all genes into ortholog groups (OG) with OrthoMCL and OrthoMCL-DB [45, 46]. The orthology relationship of the genes in the four species was then utilized to assess the interspecific divergence of predicted TFBSs, coding sequences and introns of the same genes (Additional file 2). The divergence analysis was based on Tajima’s D statistics as implemented in VariScan [47, 48]. For its input, multiple sequence alignment was performed for sequences in each OG with ClustalW2 .
The estimated intraspecific diversity of TFBSs may reflect the DNA-binding preference within TF families, whereas the estimated interspecific divergence may aid in our understanding the evolution of gene regulation. Thus, we analyzed the TFBS diversity of these genes within and between these four species using Tajima’s D statistics. A negative D value would be indicative of more conservative (or specific) binding sites than a zero or positive D value . Thus the estimates of Tajima’s D statistics here simply served to summarize the predicted TFBS motif variation within and among the four plant species rather than demographic and evolutionary inferences as often intended in the use of Tajima’s D statistics.
Mapping TFBSs onto flowering pathways
Given the obvious differences in flowering time regulation by PH, VE and PI pathways between Arabidopsis and the cereal plants (cf. Fig. 2 of ), we mapped the predicted TFBSs onto the appropriate pathways through tracking the regulated genes along the routes of the pathways. This mapping was done to infer about the species differences in promoter-driven regulation of gene expression for triggering flowering pathways and responding to environmental stimuli such as cold and long days. We used the Arabidopsis flowering pathways (WP622) in WikiPathways as a template and incorporated the known differences with the cereal species [5, 6, 8, 9] to produce a new set of pathway network charts using PathVisio (version 3.2.1), a pathway analysis tool [50, 51].
The homologous genes in the three cereal species were found using the orthology relationship (Additional file 2). Three separate pathway network charts were produced for the allohexaploid wheat, each representing one of the three homoeologs, A, B and D genomes (paralogs arising from polyploidy). By matching with the same flowering genes for TFBS prediction (see Additional file 3), we mapped our predicted TFBSs of the corresponding genes on the pathway network charts.
Genes in photoperiod, vernalization and pathway integration and their promoter regions
Numbers of homologous genes and promoters in photoperiod (PH), vernalization (VE), and pathway integration (PI) in Arabidopsis, Brachypodium, wheat and barley. Numbers in the parentheses indicate average promoter sequence length
GC-content at predicted TFBSs
In anticipation of marked interspecific difference in GC content in the promoter regions, we created the species-specific background models for Arabidopsis, Brachypodium, wheat and barley (Additional file 1: Table S1), instead of the default background model of FIMO  for TFBS prediction. We predicted 1106, 1411, 1547, and 867 TFBSs in the promoter regions of the PH, VE and PI genes in Arabidopsis, Brachypodium, wheat and barley, respectively (Additional file 3). Due to the different lengths of the promoter sequences used in each species (Table 1), the TFBS density (number of TFBSs per KB sequences) differed among the four species: 21.9 (Arabidopsis), 25.3 (Brachypodium), 12.0 (wheat), and 17.8 (barley).
The average GC percentages (± standard deviations) in different genomic regions of flowering genes in the three pathways of photoperiod (PH), vernalization (VE) and pathway integration (PI) in Arabidopsis, Brachypodium, wheat and barley. Abbreviations: TFBS: transcription factor binding site; CDS, coding sequence; UTR, untranslated region; NA, not applicable (as only one 5’ UTR sequence was found in PI genes of Brachypodium)
33.4 ± 4.3
34.2 ± 8.7
36.7 ± 4.4
45.4 ± 3.3
31.7 ± 2.6
32.5 ± 3.9
31.3 ± 3.3
32.1 ± 7.0
39.0 ± 4.0
43.3 ± 2.1
31.4 ± 2.5
33.5 ± 4.0
30.0 ± 3.4
34.1 ± 11.6
34.4 ± 2.5
46.8 ± 3.8
28.9 ± 1.8
31.9 ± 4.5
48.9 ± 7.0
47.3 ± 9.5
57.4 ± 5.3
51.9 ± 9.6
38.4 ± 3.0
41.6 ± 3.9
46.9 ± 8.4
45.0 ± 7.8
62.1 ± 4.9
49.4 ± 6.1
38.8 ± 1.7
41.1 ± 3.2
50.0 ± 9.0
46.3 ± 14.2
50.3 ± NA
64.8 ± 7.8
41.2 ± 2.5
41.5 ± 3.9
41.8 ± 8.2
43.3 ± 11.6
48.6 ± 11.3
51.0 ± 6.9
38.9 ± 3.6
43.2 ± 4.3
43.8 ± 7.7
46.4 ± 13.7
46.0 ± 12.2
48.5 ± 7.0
39.4 ± 4.7
39.4 ± 5.8
43.0 ± 11.0
47.4 ± 18.6
50.8 ± 9.9
61.3 ± 9.0
41.6 ± 6.7
41.4 ± 5.1
42.9 ± 7.8
47.4 ± 12.6
54.8 ± 10.7
52.5 ± 7.3
38.9 ± 4.6
42.7 ± 5.4
39.9 ± 5.0
40.8 ± 14.4
46.6 ± 10.6
51.1 ± 6.0
37.8 ± 2.2
39.2 ± 6.3
45.3 ± 8.5
42.5 ± 21.7
46.3 ± 10.5
62.8 ± 9.0
43.3 ± 2.7
44.3 ± 8.6
Numbers of putative transcription factor binding sites (TFBSs) and genes for the major transcription factor (TF) families in Arabidopsis, Brachypodium, wheat and barley. The TF families were sorted in descending order of the number of PWMs used in the TFBS prediction
No. TFBSs (genes)
It is also evident from Table 3 that the numbers of flowering genes used to predict TFBSs for different TF families showed some interesting contrasts between Arabidopsis and the three cereal species. For example, for the MADS-box TFs, the percentages of flowering genes used to predict TFBSs were high across all the species: 61, or 92 % of total genes examined in Arabidopsis, 57 or 95 % in Brachypodium, 131 or 88 % in wheat, and 52 or 88 % in barley. On the other hand, for the AP2 family, which is referred to as the AP2/EREBP (ethylene-responsive element binding protein), the percentages of flowering genes used for TFBS prediction varied considerably among the species: only eight genes or 12 % of total genes examined in Arabidopsis, 54 or 90 % in Brachypodium, 46 or 31 %, in wheat, and 33 or 56 % in barley. Overall, there is little relationship between the number of genes used for TFBS prediction and the size of a TF family in individual species. Most noticeably, while NAC and WRKY are large TF families in Arabidopsis , the number of genes were limited.
There were overlaps between some predicted TFBSs for the same or different TF families within the same promoter region of a given gene. The average numbers of average of TFBSs were: 12 or 68 % of total predicted TFBSs in Arabidopsis, 15 or 63 % in Brachypodium, 6 or 58 % in wheat, and 10 or 62 % in barley. The overlapping occurred in six different TF families with five in Arabidopsis. Additionally, these TFBS overlaps occur most frequently in the MADS box and CSD TF families (Additional file 4).
Relationships between TFBS numbers and gene expression profiles
Intraspecific and interspecific TFBS divergence
The interspecific diversity analysis indicated that the average D was -0.80 (-1.62 to 1.45) for TFBS, -1.08 (-1.51 to -0.36) for coding sequences (CDS), and -0.93 (-1.54 to 0.08) for introns (Additional file 1: Figure S1). On average, the observed interspecific divergence for TFBSs exceeded that of coding sequences. The relative conservation of CDS across these species was expected, as CO (AT5G15840) and CONSTANS-LIKE 2 (COL2, AT3G02380 genes are involved in the critical node (circadian rhythm) of the photoperiod pathway. However, the TFBSs had a large range of Tajima’s D, suggesting that some of them would be more divergent than others. It is of interest to note that the divergence of introns of these genes between these species as judged from its D value lied intermediate between the estimates of the divergence for TFBS and CDS (Additional file 6).
TFBS mapping over the flowering pathways
For those genes found in the flowering pathways, the number of TFBSs of the same genes varied considerably among the species (Fig. 4). In all four species, the predicted TFBSs were most frequent for the MADS-box and CSD family TFs that regulate the photoreceptor genes in the phtoperiod pathway. However, there were exceptions, e.g. PHYD in the Arabidopsis wherethe TFBSs for the bHLH and bZIP family TFs were most frequent. another noticveable difference between the Arabidopsis and cereals is that the AP2 TFs were frequent participants of DNA binding in the cereals particularly in Brachypodium but almost absent in the Arabidopsis. Similar patterns of TFBS distributions were found in the vernalization pathway with the predicted TFBSs for MADS-box and CSD family TFs being most frequent across all the species. it appeared that the cereal VRN1 (homologous to APETALA 1 (AP1)/CAULIFLOWER (CAL)/FRUITFULL (FUL), not VRN1 in Arabidopsis) and VRN4 (no Arabidopsis equivalent found in Arabidopsis), AP2 family TFs were important contributors to DNA binding for vernalization regulation, judging from the frequencies of their TFBSs we predicted. For the pathway integrator genes such as FLOWERING LOCUS T (FT), SOC1, AGAMOUS-LIKE 24 (AGL24), FUL and LFY, the patterns similar to those found in the photoperiod and vernalization pathways appeared again: the most frequent occurrence of TFBSs for MADS-box and CSD family TFs in Arabidopsis and cereals, but the equal frequent occurrence of TFBSs for AP2 family TFs in the cereals only.. For example, of the 23 TFBSs predicted for the FT gene in Arabidopsis, 12 (52 %) were for CSD TFs and 10 (43 %) were for MADS-box TFs. In the three cereal species, on the other hand, while MADS-box (and to a lesser extent, CSD) remained to be the main TF families for the predicted TFBSs, AP2 become another major TF family for regulating the pathway integration particularly in Brachypodium and barley. For example, of the 30 TFBSs predicted in Brachypodium for the FT1gene, 16 (or 53 %) were for AP2 TFs, seven (23 %) for MADS-box TFs and the rest were for the STK and ATH TFs.
In this work, we predicted TFBSs in the promoter regions of flowering genes involved in the PH, VE and PI pathways in two model plants, Arabidopsis and Brachypodium, and two important cereal crops, wheat and barley. We chose the two major flowering pathways (PH and VE) and their integrator (PI) for TFBS prediction because these pathways are regulated by the well-known gene regulatory networks [3, 6, 54], but little experimental evidence is available for characterizing how cis- regulatory elements (CREs) in the promoter regions of the genes are interacted with each other to activate or repress the expression of a network of genes along the pathways. For easy identification and comarison within and among the four species, we mapped the predicted TFBSs onto appropriate flowering genes present in the pathways (Fig. 4). In this fahsion, a gene regulatory network is readily formed to visualize the flow of gene regulation within the network (i.e., how one gene regulator is controlled by another in the network of genes within and between the pathways). To the best of our knowledge, this is the first attempt to link the pathways with predicted TFBSs, thereby providing an opportunity for pathway-guided prediction of TFs for specific genes in future studies.
Recent sequencing of several genomes of non-model plants including the large and complex genomes of wheat and barley [52, 55, 56] has allowed for prediction or identification of important genes (e.g., flowering genes) in the crop species based on well-annotated genes in model species such as Arabidopsis or Brachypodium. However, these gene annotation data can only be more effectively utilized if more is learned from patterns and properties of CREs. In our study, we focused on predicting distributions and patterns of TFBSs in the promoter regions of flowering genes. While the promoters are essential for transcription regulation of flowering or other functional genes across the genome, they alone can only produce basal levels of mRNA. Additionally, TFBSs in promoter regions often bind to a set of widely used and highly conserved TFs and thus they are not the major cause of cis-regulatory divergence among different species. In contrast, enhancers often turn on the promoters at specific genomic locations, times, and levels, and that is why they are sometimes known as the “promoters of the promoter.” Enhancers exhibit more interspecific variability and thus they are more often considered to be responsible for cis-regulatory divergence. However, unlike promoters, enhancers are more difficult to be located because they appear to be in upstream (5’), downstream (3’) or in the intron(s) of the gene they regulate; they can also be located far away from the gene.
Recently, a new open chromatin signature-based enhancer prediction system was developed for enhancer identification in Arabidopsis and other plants  Genome-wide patterns and distributions of other types of CREs such as silencers and insulators remain poorly understood. Thus, future studies can identify reproducible sequence patterns and genomic locations of TFBSs for enhancers and other less well-characterized CREs for bioinformatic predictions similar to what we did for promoters in this study.
While TFBS prediction is an important first step towards molecular characterization of gene regulation in plant species, it remains more difficult than gene prediction for the following reasons. First, regulatory regions control the transcription of genes but do not directly code for an identifiable product or function. Thus, TFBSs must be predicted from DNA sequences alone. Second, TFBSs are typically short in length, ranging from 5 to 31 nucleotides with an average length of merely 10 bp in eukaryotes . Hence, it can be difficult to predict TFBSs using simple sequence analysis tools such as BLAST. Third, most TFBSs are highly degenerate [59, 60], which are reflected in the different probabilities of the four nucleotides at each position of PWMs. Consequently, a similar promoter sequence can be recognized by different groups of TFs and a TF may bind to more than one motif . To further improve the quality of the predicted TFBSs, we implemented several filtering steps for both input and output files. First, those promoter sequences with large gaps (N’s). were removed from the input file. Second, high-quality PWMs (preferably derived from direct experimental data) were used for TFBS prediction. PWMs from public databases, such as JASPAR, TRANSFAC and CIS-BP [24, 30, 31], might be redundant. For example, even in the same database, identical TFs may sometimes be represented by different matrices that are obtained with different methods . Consequently, redundant or very similar PWMs were removed to reduce the false positive rate. Third, for the FIMO output files, the predicted motifs were examined to only retain the most significant TFBSs or the longest motif covering short ones within the same TF family.
The largest numbers of TFBSs were predicted for MADS-box and CSD TF families, suggesting their important roles in flowering regulation. Several reasons for such TFBS motif abundance in MADS-box and CSD TF families may be speculated. First of all, the large number of hits may be due to the possibility that the PWMs for these TF families are less conservative. To check out this possibility, we calculated the Kullback–Leibler divergence (DKL) indices  for all 371 PWMs, corresponding to 345 cognate TFs. The DKL values had a wide range from -0.2179 to -6.5575. A DKL index should be close to zero if the letter (basepair) distribution is close to a uniform distribution (i.e., p- > 0.25); otherwise it would be far from zero. According to this criterion, the MADS-CSD motifs are actually more conservative as their DKL values were < -4.0. So the large number of hits in TFBS motif search is not necessarily caused by the less conservation of PWMs for the MADS-CSD motifs.
There are other possible reasons for the TFBS motif abundance in MADS-box and CSD TF families as well. The roles of MADS-box TFs in flowering control have long been established, and CSD TFs are mainly involved in cold acclimation but some of them are also related to flowering time [64–70]. Furthermore, most MADS-box and CSD motifs overlap in the promoter region of a target gene, suggesting that they might play cooperative functions in the regulation of photoperiod and vernalization responses. Additionally, we found genes with similar number of MADS-box and CSD TFBSs often show similar expression (coexpression) patterns in different tissues and developmental stages. It is somewhat surprising that we predicted the highest density of putative TFBSs in Brachypodium. This might be at least partially due to the larger number (195) of PWMs derived from monocot species than the number (176) of PWMs from Arabidopsis. Another reason might be the higher quality of promoter sequences in Brachypodium than in wheat and barley. The functional implication of higher TFBS density in Brachypodium may be a topic for further research.
It should be noted that only a limited number of PWMs are available in the two monocot crop species in our study, wheat and barley, because few functional genes such as those related to flowering are molecularly characterized in these large, complex genomes. The TFBS prediction based on very few PWMs would be unreliable. For this reason, maize, along a few other species, was added to the list of the monocot species in our initial compiling of PWMs. In particular, maize had 209 PWMs compared to nine PWMs for wheat and six PWMs for barley. Brachypodium is phylogenetically closer to wheat and barley than maize and had a similar number (192) of PWMs to maize, but it is a wild species with a potentially large number of ancient or doimestication genes that have been eliminated or modified from the geneomes of maize, wheat and barley during their domestication and selective breeding . A check based on the DKL index  shows a similarity between motifs of maize, wheat and barley. Thus adding maize to the list would have helped to improve the accuracy of the TFBS prediction through borrawing the PWM information from the monocot species such as maize with close phylogeny and similar demostication levels.
The superimposition of TFBS numbers of the major TF families in the flowering pathways allows us to compare the TFBS profiles in promoters of the regulated genes for PH, VE and PI pathways in the four species. For the orthologous genes we determined among the four these species, both the similarity and differences were found. For example, TFBSs of MADS-box TFs were predicted for the orthologous photoperiod gene CO in the four species. On the other hand, the floral integrator FT gene showed different TFBS profiles. For example, the Arabidopsis FT gene was predicted to be regulated by CSD and MADS-box TFs. However, both the Brachypodium FT (BRADI1G48830) and barley FT gene (VRN3-H, MLOC_68576) appeared to be regulated by TFs in AP2, MADS and STK (storekeeper) families. The putative wheat FT1-A (Traes_7AS_EBD5F1F54) might be controlled by storekeeper (STK), MADS and AT hook (ATH) TF families, similar to FT1-B (Traes_7BS_581AA844D) and FT1-D (Traes_7DS_12C14942B, though only had one predicted TFBS each for ATH and STK family TFs). This difference of transcriptional regulation in FT genes may be supported by the view in : the roles of FT-like genes appear to be highly conserved, but the TFs controlling their transcription vary during evolution, allowing transcription of FT-like genes in response to different conditions. Overall, the relative similarity of TFBS profiles in PH, VE and PI pathways are consistent with the conservation and divergence of these flowering pathways between the species [6, 8, 11].
Our GC content analysis was used as an indicator of promoters, because previous studies of regulatory sequences suggested that GC content has a significant difference between dicots and cereals, and methods of promoter identification based on DNA free energy profile were developed [32, 33]. Additionally, TFs from different families often prefer binding to regions with low or high GC content surrounding the core TFBS , thus it would be interesting to examine how the different GC content in the promoter regions of these genes might affect the binding environment of different TFs. In flower development, epigenetic regulation such as DNA methylation, histone modification, nucleosome positioning, and chromatin accessibility also plays an important role [21, 73–75]. Thus, it is possible that the different GC content in the promoter regions (particularly those surrounding predicted TFBSs) between Arabidopsis and cereal species might affect TF binding via epigenetic mechanisms.
The TFBS divergence relative to their corresponding coding sequences and introns was assessed using the Tajima test in VariScan [47, 48]. The Tajima’s D has previously been used to assess divergence patterns in the conifer EST (expressed sequence tag) data . Our analysis suggests different binding preference of each TF family in each species, including MADS-box and CSD. And among these four species, the TFBSs diverged faster than their corresponding CDS, similar to the binding sites of Ste12 and Tec1 regulators in yeast . More interestingly, introns might also diverge faster than CDS (but slower than TFBS). The potential function of introns in TFBSs and flowering regulation has gained growing attention [22, 23, 78, 79].
Despite its popularity for TFBS predictions, FIMO, like most motif search tools, may be prone to a high rate of false positives. There is definitely a need to further check the validity of FIMO-based predictions. We have tried out some recently-developed tools for comparative assessment with FIMO. In particular, BoBro 2.0 of Ma et al.  is an integreated toolkit aiming at improved control of false positive rate for the predicted TFBS motifs and higher prediction sensitivity through efficient handling of sequence variation in motifs. The preliminary results show that some TFBS motifs predicted by both FIMO and BoBro 2.0 have overlapping but not identical start/end positions while the majority of others are located at separate regions of the genome. Since BoBro 2.0 was developed initially for prokarotic genomes, further investigations are needed to make BoBro 2.0 or similar tools well adapted to the TFBS predictions for eukarotic genomes of higher plants such as those in our study.
Our FIMO-based TFBS motif search is based on known binding sites. When such knowledge is not available, de nevo motif discovery  has been suggested as an alternative approach to predicting TFBS motifs. In the de nevo motif discovery, multiple sequences are input to detect one or more candidate motifs. However, while the use of de novo motif search could have allowed us to find many more motifs, it would have been difficult to associate the motifs discovered de novo with known TFs. Without knowledge of their potential TFs, the predicted motifs would be of very limited value. Even though the de nevo motif search was not used in our study, it certainly needs to be explored in future studies.
Using the FIMO motif discovery tool in MEMPpE , we predicted a large number of putative TFBSs in the promoters of the genes related to the PH, VE and PI pathways in Arabidopsis, Brachypodium, wheat and barley. The quality of the predicted TFBSs was improved through cleaning both the inputs (promoter sequences and PWMs) and the FIMO outputs. The genes with similar TFBS numbers tend to be co-expressed in different tissues of each species. Based on our intraspecific and interspecific Tajima D-statistics [47, 48], TFBSs from different TF families showed different divergence within each species, and TFBSs are more divergent compared with CDS and introns. The TFBS numbers for major TF families were superimposed in the flowering pathways with PathVisio and WikiPathways [50, 51], to show the similarity and difference between these four species. The TFBSs and TF-targeted gene associations presented in our study can be investigated for their roles in photoperiod and vernalization responses in the genomes of four plant species, especially in the large, poorly-characterized genomes of two cereal crops, wheat and barley.
We thank Dr. Paul Stothard for helpful discussion at the initial stage of this research.
This research is funded by the Growing Forward 2 Research Opportunities and Innovation Internal Initiatives of Alberta Agriculture and Forestry to R-C Yang.
Availability of data and material
The datasets supporting the conclusions of this article are included within the article and its additional files.
FYP carried out bioinformatics analysis and prepared the first draft of the manuscript. ZQH performed PWM filtering and statistical analysis. R-CY conceived the project, supervised the analysis and revised the manuscript. All authors read and approved the final version of the manuscript.
The authors declare that they have no competing interests.
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- Fornara F, de Montaigu A, Coupland G. SnapShot: Control of flowering in Arabidopsis. Cell. 2010;141(3):550. doi:10.1016/j.cell.2010.04.024.View ArticlePubMedGoogle Scholar
- Brachi B, Faure N, Horton M, Flahauw E, Vazquez A, Nordborg M, et al. Linkage and association mapping of Arabidopsis thaliana flowering time in nature. PLoS Genet. 2010;6(5):e1000940. doi:10.1371/journal.pgen.1000940.View ArticlePubMedPubMed CentralGoogle Scholar
- Higgins JA, Bailey PC, Laurie DA. Comparative genomics of flowering time pathways using Brachypodium distachyon as a model for the temperate grasses. PLoS One. 2010;5(4):e10065. doi:10.1371/journal.pone.0010065.View ArticlePubMedPubMed CentralGoogle Scholar
- Peng FY, Hu Z, Yang R-C. Genome-wide comparative analysis of flowering-related genes in Arabidopsis, wheat, and barley. Int J Plant Genomics. 2015;2015:874361. doi:10.1155/2015/874361.View ArticlePubMedPubMed CentralGoogle Scholar
- Distelfeld A, Li C, Dubcovsky J. Regulation of flowering in temperate cereals. Curr Opin Plant Biol. 2009;12(2):178–84. http://dx.doi.org/10.1016/j.pbi.2008.12.010.View ArticlePubMedGoogle Scholar
- Andres F, Coupland G. The genetic basis of flowering responses to seasonal cues. Nat Rev Genet. 2012;13(9):627–39. doi:10.1038/Nrg3291.View ArticlePubMedGoogle Scholar
- Shrestha R, Gomez-Ariza J, Brambilla V, Fornara F. Molecular control of seasonal flowering in rice, arabidopsis and temperate cereals. Ann Bot-London. 2014;114(7):1445–58. doi:10.1093/aob/mcu032.View ArticleGoogle Scholar
- Blumel M, Dally N, Jung C. Flowering time regulation in crops-what did we learn from Arabidopsis? Curr Opin Biotechnol. 2015;32:121–9. doi:10.1016/j.copbio.2014.11.023.View ArticlePubMedGoogle Scholar
- Fjellheim S, Boden S, Trevaskis B. The role of seasonal flowering responses in adaptation of grasses to temperate climates. Front Plant Sci. 2014;5:431. doi:10.3389/Fpls.2014.00431.View ArticlePubMedPubMed CentralGoogle Scholar
- Song YH, Ito S, Imaizumi T. Similarities in the circadian clock and photoperiodism in plants. Curr Opin Plant Biol. 2010;13(5):594–603. doi:10.1016/j.pbi.2010.05.004.View ArticlePubMedPubMed CentralGoogle Scholar
- Calixto CPG, Waugh R, Brown JWS. Evolutionary relationships among barley and Arabidopsis core circadian clock and clock-associated genes. J Mol Evol. 2015;80(2):108–19. doi:10.1007/s00239-015-9665-0.View ArticlePubMedPubMed CentralGoogle Scholar
- Wittkopp PJ, Kalay G. Cis-regulatory elements: molecular mechanisms and evolutionary processes underlying divergence. Nat Rev Genet. 2012;13(1):59–69. http://www.nature.com/nrg/journal/v13/n1/full/nrg3095.html.View ArticleGoogle Scholar
- Beales J, Turner A, GriYths S, Snape JW, Laurie DA. A Pseudo-Response Regulator is misexpressed in the photoperiod insensitive Ppd-D1a mutant of wheat (Triticum aestivum L.). Theor Appl Genet. 2007;115(5):721–33. doi:10.1007/s00122-007-0603-4.View ArticlePubMedGoogle Scholar
- Wilhelm EP, Turner AS, Laurie DA. Photoperiod insensitive Ppd-A1a mutations in tetraploid wheat (Triticum durum Desf.). Theor Appl Genet. 2009;118(2):285–94. doi:10.1007/s00122-008-0898-9.View ArticlePubMedGoogle Scholar
- Shaw LM, Turner AS, Herry L, Griffiths S, Laurie DA. Mutant alleles of Photoperiod-1 in wheat (Triticum aestivum L.) that confer a late flowering phenotype in long days. PLoS One. 2013;8(11):e79459. doi:10.1371/journal.pone.0079459.View ArticlePubMedPubMed CentralGoogle Scholar
- Nishida H, Yoshida T, Kawakami K, Fujita M, Long B, Akashi Y, et al. Structural variation in the 5’ upstream region of photoperiod-insensitive alleles Ppd-A1a and Ppd-B1a identified in hexaploid wheat (Triticum aestivum L.), and their effect on heading time. Mol Breed. 2013;31(1):27–37. doi:10.1007/s11032-012-9765-0.View ArticleGoogle Scholar
- Yan L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J. Positional cloning of the wheat vernalization gene VRN1. Proc Natl Acad Sci U S A. 2003;100(10):6263–8. doi:10.1073/pnas.0937399100.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang J, Wang YY, Wu SW, Yang JP, Liu HW, Zhou Y. A single nucleotide polymorphism at the Vrn-D1 promoter region in common wheat is associated with vernalization response. Theor Appl Genet. 2012;125(8):1697–704. doi:10.1007/s00122-012-1946-z.View ArticlePubMedGoogle Scholar
- Cockram J, Chiapparino E, Taylor SA, Stamati K, Donini P, Laurie DA, et al. Haplotype analysis of vernalization loci in European barley germplasm reveals novel VRN-H1 alleles and a predominant winter VRN-H1/VRN-H2 multi-locus haplotype. Theor Appl Genet. 2007;115(7):993–1001. doi:10.1007/s00122-007-0626-x.View ArticlePubMedGoogle Scholar
- Fu DL, Szucs P, Yan LL, Helguera M, Skinner JS, von Zitzewitz J, et al. Large deletions within the first intron in VRN-1 are associated with spring growth habit in barley and wheat (vol 273, pg 54, 2005). Mol Genet Genomics. 2005;274(4):442–3. doi:10.1007/s00438-005-0045-0.View ArticleGoogle Scholar
- Oliver SN, Deng WW, Casao MC, Trevaskis B. Low temperatures induce rapid changes in chromatin state and transcript levels of the cereal VERNALIZATION1 gene. J Exp Bot. 2013;64(8):2413–22. doi:10.1093/jxb/ert095.View ArticlePubMedPubMed CentralGoogle Scholar
- Kippes N, Debernardi JM, Vasquez-Gross HA, Akpinar BA, Budak H, Kato K, et al. Identification of the VERNALIZATION 4 gene reveals the origin of spring growth habit in ancient wheats from South Asia. Proc Natl Acad Sci U S A. 2015;112(39):E5401–10. doi:10.1073/pnas.1514883112.View ArticlePubMedPubMed CentralGoogle Scholar
- Park SG, Hannenhalli S, Choi SS. Conservation in first introns is positively associated with the number of exons within genes and the presence of regulatory epigenetic signals. BMC Genomics. 2014;15:526. doi:10.1186/1471-2164-15-526.View ArticlePubMedPubMed CentralGoogle Scholar
- Weirauch MT, Yang A, Albu M, Cote AG, Montenegro-Montero A, Drewe P, et al. Determination and inference of eukaryotic transcription factor sequence specificity. Cell. 2014;158(6):1431–43. doi:10.1016/j.cell.2014.08.009.View ArticlePubMedPubMed CentralGoogle Scholar
- Franco-Zorrilla JM, Lopez-Vidriero I, Carrasco JL, Godoy M, Vera P, Solano R. DNA-binding specificities of plant transcription factors and their potential to define target genes. Proc Natl Acad Sci U S A. 2014;111(6):2367–72. doi:10.1073/pnas.1316278111.View ArticlePubMedPubMed CentralGoogle Scholar
- Farnham PJ. Insights from genomic profiling of transcription factors. Nat Rev Genet. 2009;10(9):605–16. doi:10.1038/nrg2636.View ArticlePubMedPubMed CentralGoogle Scholar
- Mathelier A, Wasserman WW. The next generation of transcription factor binding site prediction. PLoS Comput Biol. 2013;9(9):e1003214. doi:10.1371/journal.pcbi.1003214.View ArticlePubMedPubMed CentralGoogle Scholar
- Grant CE, Bailey TL, Noble WS. FIMO: scanning for occurrences of a given motif. Bioinformatics. 2011;27(7):1017–8. doi:10.1093/bioinformatics/btr064.View ArticlePubMedPubMed CentralGoogle Scholar
- Stormo GD. Modeling the specificity of protein-DNA interactions. Quant Biol. 2013;1(2):115–30. doi:10.1007/s40484-013-0012-4.View ArticlePubMedPubMed CentralGoogle Scholar
- Mathelier A, Zhao XB, Zhang AW, Parcy F, Worsley-Hunt R, Arenillas DJ, et al. JASPAR 2014: an extensively expanded and updated open-access database of transcription factor binding profiles. Nucleic Acids Res. 2014;42:D142–7. doi:10.1093/nar/gkt997.View ArticlePubMedGoogle Scholar
- Matys V, Kel-Margoulis OV, Fricke E, Liebich I, Land S, Barre-Dirrie A, et al. TRANSFAC and its module TRANSCompel: transcriptional gene regulation in eukaryotes. Nucleic Acids Res. 2006;34:D108–10. doi:10.1093/nar/gkj143.View ArticlePubMedGoogle Scholar
- Kumari S, Ware D. Genome-wide computational prediction and analysis of core promoter elements across plant monocots and dicots. PLoS One. 2013;8(10):e79011. doi:10.1371/journal.pone.0079011.View ArticlePubMedPubMed CentralGoogle Scholar
- Morey C, Mookherjee S, Rajasekaran G, Bansal M. DNA free energy-based promoter prediction and comparative analysis of Arabidopsis and rice genomes. Plant Physiol. 2011;156(3):1300–15. doi:10.1104/pp.110.167809.View ArticlePubMedPubMed CentralGoogle Scholar
- Yilmaz A, Mejia-Guerra MK, Kurz K, Liang XY, Welch L, Grotewold E. AGRIS: the Arabidopsis gene regulatory information server, an update. Nucleic Acids Res. 2011;39:D1118–22. doi:10.1093/nar/gkq1120.View ArticlePubMedGoogle Scholar
- Kaufmann K, Pajoro A, Angenent GC. Regulation of transcription in plants: mechanisms controlling developmental switches. Nat Rev Genet. 2010;11(12):830–42. doi:10.1038/nrg2885.View ArticlePubMedGoogle Scholar
- Altschul SF, Madden TL, Schaffer AA, Zhang JH, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997;25(17):3389–402. doi:10.1093/nar/25.17.3389.View ArticlePubMedPubMed CentralGoogle Scholar
- Medina-Rivera A, Defrance M, Sand O, Herrmann C, Castro-Mondragon JA, Delerce J, et al. RSAT 2015: regulatory sequence analysis tools. Nucleic Acids Res. 2015;43:W50–6. doi:10.1093/nar/gkv362.View ArticlePubMedPubMed CentralGoogle Scholar
- Cunningham F, Amode MR, Barrell D, Beal K, Billis K, Brent S, et al. Ensembl 2015. Nucleic Acids Res. 2015;43:D662–9. doi:10.1093/Nar/Gku1010.View ArticlePubMedGoogle Scholar
- Bailey TL, Boden M, Buske FA, Frith M, Grant CE, Clementi L, et al. MEME SUITE: tools for motif discovery and searching. Nucleic Acids Res. 2009;37:W202–8. doi:10.1093/nar/gkp335.View ArticlePubMedPubMed CentralGoogle Scholar
- Berger MF, Bulyk ML. Universal protein-binding microarrays for the comprehensive characterization of the DNA-binding specificities of transcription factors. Nat Protoc. 2009;4(3):393–411. doi:10.1038/nprot.2008.195.View ArticlePubMedPubMed CentralGoogle Scholar
- R Core Team. R: A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing; 2014.Google Scholar
- Storey JD. The positive false discovery rate: A Bayesian interpretation and the q-value. Ann Stat. 2003;31(6):2013–35. doi:10.1214/aos/1074290335.View ArticleGoogle Scholar
- Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, et al. Bioconductor: open software development for computational biology and bioinformatics. Genome Biol. 2004;5(10):R80. doi:10.1186/Gb-2004-5-10-R80.View ArticlePubMedPubMed CentralGoogle Scholar
- Zhao S, Fung-Leung W-P, Bittner A, Ngo K, Liu X. Comparison of RNA-Seq and Microarray in Transcriptome Profiling of Activated T Cells. PLoS One. 2014;9(1):e78644. doi:10.1371/journal.pone.0078644.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen F, Mackey AJ, Stoeckert CJ, Roos DS. OrthoMCL-DB: querying a comprehensive multi-species collection of ortholog groups. Nucleic Acids Res. 2006;34:D363–8. doi:10.1093/Nar/Gkj123.View ArticlePubMedGoogle Scholar
- Li L, Stoeckert CJ, Roos DS. OrthoMCL: Identification of ortholog groups for eukaryotic genomes. Genome Res. 2003;13(9):2178–89. doi:10.1101/Gr.1224503.View ArticlePubMedPubMed CentralGoogle Scholar
- Tajima F. Statistical Method for Testing the Neutral Mutation Hypothesis by DNA Polymorphism. Genetics. 1989;123(3):585–95.PubMedPubMed CentralGoogle Scholar
- Vilella AJ, Blanco-Garcia A, Hutter S, Rozas J. VariScan: Analysis of evolutionary patterns from large-scale DNA sequence polymorphism data. Bioinformatics. 2005;21(11):2791–3. doi:10.1093/bioinformatics/bti403.View ArticlePubMedGoogle Scholar
- Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, et al. Clustal W and Clustal X version 2.0. Bioinformatics. 2007;23(21):2947–8. doi:10.1093/bioinformatics/btm404.View ArticlePubMedGoogle Scholar
- Kelder T, van Iersel MP, Hanspers K, Kutmon M, Conklin BR, Evelo CT, et al. WikiPathways: building research communities on biological pathways. Nucleic Acids Res. 2012;40:D1301–7. doi:10.1093/nar/gkr1074.View ArticlePubMedGoogle Scholar
- Kutmon M, van Iersel MP, Bohler A, Kelder T, Nunes N, Pico AR, et al. PathVisio 3: an extendable pathway analysis toolbox. PLoS Comput Biol. 2015;11(2):e1004085. doi:10.1371/journal.pcbi.1004085.View ArticlePubMedPubMed CentralGoogle Scholar
- Brenchley R, Spannagl M, Pfeifer M, Barker GLA, D/’Amore R, Allen AM, et al. Analysis of the bread wheat genome using whole-genome shotgun sequencing. Nature. 2012;491(7426):705–10. http://www.nature.com/nature/journal/v491/n7426/full/nature11650.html.View ArticlePubMedPubMed CentralGoogle Scholar
- Riechmann JL, Ratcliffe OJ. A genomic perspective on plant transcription factors. Curr Opin Plant Biol. 2000;3(5):423–34.View ArticlePubMedGoogle Scholar
- Ream TS, Woods DP, Schwartz CJ, Sanabria CP, Mahoy JA, Walters EM, et al. Interaction of photoperiod and vernalization determines flowering time of Brachypodium distachyon. Plant Physiol. 2014;164(2):694–709. doi:10.1104/pp.113.232678.View ArticlePubMedGoogle Scholar
- The International Barley Genome Sequencing Consortium. A physical, genetic and functional sequence assembly of the barley genome. Nature. 2012;491(7426):711–6. http://www.nature.com/nature/journal/v491/n7426/full/nature11543.html.Google Scholar
- The International Wheat Genome Sequencing Consortium. A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome. Science. 2014;345(6194):1251788. doi:10.1126/science.1251788.View ArticleGoogle Scholar
- Zhu B, Zhang W, Zhang T, Liu B, Jiang J. Genome-Wide Prediction and Validation of Intergenic Enhancers in Arabidopsis Using Open Chromatin Signatures. Plant Cell. 2015;27:2415–26. doi:10.1105/tpc.15.00537.View ArticlePubMedPubMed CentralGoogle Scholar
- Stewart AJ, Hannenhalli S, Plotkin JB. Why transcription factor binding sites are ten nucleotides long. Genetics. 2012;192(3):973–85. doi:10.1534/genetics.112.143370.View ArticlePubMedPubMed CentralGoogle Scholar
- Doniger SW, Huh J, Fay JC. Identification of functional transcription factor binding sites using closely related Saccharomyces species. Genome Res. 2005;15(5):701–9. doi:10.1101/gr.3578205.View ArticlePubMedPubMed CentralGoogle Scholar
- Oh YM, Kim JK, Choi S, Yoo JY. Identification of co-occurring transcription factor binding sites from DNA sequence using clustered position weight matrices. Nucleic Acids Res. 2012;40(5):e38. doi:10.1093/nar/gkr1252.View ArticlePubMedGoogle Scholar
- Badis G, Berger MF, Philippakis AA, Talukder S, Gehrke AR, Jaeger SA, et al. Diversity and complexity in DNA recognition by transcription factors. Science. 2009;324(5935):1720–3. doi:10.1126/science.1162327.View ArticlePubMedPubMed CentralGoogle Scholar
- Kielbasa SM, Gonze D, Herzel H. Measuring similarities between transcription factor binding sites. BMC Bioinformatics. 2005;6:237. doi:10.1186/1471-2105-6-237.View ArticlePubMedPubMed CentralGoogle Scholar
- Erill I, O’Neill MC. A reexamination of information theory-based methods for DNA-binding site identification. BMC Bioinformatics. 2009;10:57. doi:10.1186/1471-2105-10-57.View ArticlePubMedPubMed CentralGoogle Scholar
- Becker A, Theissen G. The major clades of MADS-box genes and their role in the development and evolution of flowering plants. Mol Phylogenet Evol. 2003;29(3):464–89. doi:10.1016/S1055-7903(03)00207-0.View ArticlePubMedGoogle Scholar
- Dorca-Fornell C, Gregis V, Grandi V, Coupland G, Colombo L, Kater MM. The Arabidopsis SOC1-like genes AGL42, AGL71 and AGL72 promote flowering in the shoot apical and axillary meristems. Plant J. 2011;67(6):1006–17. doi:10.1111/j.1365-313X.2011.04653.x.View ArticlePubMedGoogle Scholar
- Gu XF, Le C, Wang YZ, Li ZC, Jiang DH, Wang YQ, et al. Arabidopsis FLC clade members form flowering-repressor complexes coordinating responses to endogenous and environmental cues. Nat Commun. 2013;4:1947. doi:10.1038/Ncomms2947.PubMedPubMed CentralGoogle Scholar
- Trevaskis B, Hemming MN, Peacock WJ, Dennis ES. HvVRN2 responds to daylength, whereas HvVRN1 is regulated by vernalization and developmental status. Plant Physiol. 2006;140(4):1397–405. doi:10.1104/pp.105.073486.View ArticlePubMedPubMed CentralGoogle Scholar
- Wei B, Zhang RZ, Guo JJ, Liu DM, Li AL, Fan RC, et al. Genome-wide analysis of the MADS-box gene family in Brachypodium distachyon. PLoS One. 2014;9(1):e84781. doi:10.1371/journal.pone.0084781.View ArticlePubMedPubMed CentralGoogle Scholar
- Mihailovich M, Militti C, Gabaldon T, Gebauer F. Eukaryotic cold shock domain proteins: highly versatile regulators of gene expression. Bioessays. 2010;32(2):109–18. doi:10.1002/bies.200900122.View ArticlePubMedGoogle Scholar
- Sasaki K, Imai R. Pleiotropic roles of cold shock domain proteins in plants. Front Plant Sci. 2012;2:116. doi:10.3389/fpls.2011.00116.View ArticlePubMedPubMed CentralGoogle Scholar
- Girin T, David LC, Chardin C, Sibout R, Krapp A, Ferrario-Mery S, et al. Brachypodium: a promising hub between model species and cereals. J Exp Bot. 2014;65(19):5683–96. doi:10.1093/jxb/eru376.View ArticlePubMedGoogle Scholar
- Dror I, Golan T, Levy C, Rohs R, Mandel-Gutfreund Y. A widespread role of the motif environment in transcription factor binding across diverse protein families. Genome Res. 2015;25(9):1268–80. doi:10.1101/gr.184671.114.View ArticlePubMedPubMed CentralGoogle Scholar
- Bastow R, Mylne JS, Lister C, Lippman Z, Martienssen RA, Dean C. Vernalization requires epigenetic silencing of FLC by histone methylation. Nature. 2004;427(6970):164–7. doi:10.1038/nature02269.View ArticlePubMedGoogle Scholar
- Pajoro A, Madrigal P, Muino JM, Matus JT, Jin J, Mecchia MA, et al. Dynamics of chromatin accessibility and gene regulation by MADS-domain transcription factors in flower development. Genome Biol. 2014;15(3):R41. doi:10.1186/gb-2014-15-3-r41.View ArticlePubMedPubMed CentralGoogle Scholar
- Shi J, Dong A, Shen WH. Epigenetic regulation of rice flowering and reproduction. Front Plant Sci. 2015;5:803. doi:10.3389/fpls.2014.00803.View ArticlePubMedPubMed CentralGoogle Scholar
- Palme AE, Wright M, Savolainen O. Patterns of divergence among conifer ESTs and polymorphism in Pinus sylvestris identify putative selective sweeps. Mol Biol Evol. 2008;25(12):2567–77. doi:10.1093/molbev/msn194.View ArticlePubMedGoogle Scholar
- Borneman AR, Gianoulis TA, Zhang ZDD, Yu HY, Rozowsky J, Seringhaus MR, et al. Divergence of transcription factor binding sites across related yeast species. Science. 2007;317(5839):815–9. doi:10.1126/science.1140748.View ArticlePubMedGoogle Scholar
- Szucs P, Skinner JS, Karsai I, Cuesta-Marcos A, Haggard KG, Corey AE, et al. Validation of the VRN-H2/VRN-H1 epistatic model in barley reveals that intron length variation in VRN-H1 may account for a continuum of vernalization sensitivity. Mol Genet Genomics. 2007;277(3):249–61. doi:10.1007/s00438-006-0195-8.View ArticlePubMedGoogle Scholar
- Schauer SE, Schluter PM, Baskar R, Gheyselinck J, Bolanos A, Curtis MD, et al. Intronic regulatory elements determine the divergent expression patterns of AGAMOUS-LIKE6 subfamily members in Arabidopsis. Plant J. 2009;59(6):987–1000. doi:10.1111/j.1365-313X.2009.03928.x.View ArticlePubMedGoogle Scholar
- Ma Q, Liu B, Zhou C, Yin Y, Li G, Xu Y. An integrated toolkit for accurate prediction and analysis of cis-regulatory motifs at a genome scale. Bioinformatics. 2013;29(18):2261–8. doi:10.1093/bioinformatics/btt397.View ArticlePubMedGoogle Scholar
- Altarawy D, Ismail MA, Ghanem SM. MProfiler: A Profile-Based Method for DNA Motif Discovery. In: Kadirkamanathan V, Sanguinetti G, Girolami M, Niranjan M, Noirel J, editors. Pattern Recognition in Bioinformatics: 4th IAPR International Conference, PRIB 2009, Sheffield, UK, September 7-9, 2009. Proceedings. Berlin, Heidelberg: Springer Berlin Heidelberg; 2009. p. 13–23.View ArticleGoogle Scholar