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Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

BMC Genomics volume 21, Article number: 99 (2020) Cite this article

Abstract

Background

Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end (dnd). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon.

Results

We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf, inha, nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively.

Conclusions

This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell- and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha, are located within granulosa and Sertoli cells.

Background

Atlantic salmon is a highly valuable commercial species, to which a substantial amount of research has been dedicated during the recent years. One of the bottlenecks currently limiting the possibilities to further increase the production is the environmental concern about farmed escapees breeding with wild populations of salmon, thereby causing genetic introgression [1, 2]. One solution would be the use of sterile salmon in aquaculture, and a germ cell-free sterile salmon (GCF salmon) has indeed been produced by either knockout or knockdown of the dead end (dnd) gene [3, 4]. Ongoing studies are investigating the possibility to block the function of Dnd in salmon at the RNA level (gene knockdown) in a similar way that has been done in zebrafish (Danio rerio) [5, 6]. Since it is challenging to develop a successful sterility treatment against a gene product already present in the body, alternative novel targets are also being investigated. Studies on salmon have identified and characterized genes preferentially expressed in germ cells [7,8,9], which could potentially serve as additional targets for the blocking of germ cell development. Another possible approach is to target proteins essential for germ cell survival or fertility. Such proteins could potentially be expressed in the germ cells, such as Bmp15 or Gdf9 [10,11,12,13]. However, the somatic gonad may also confer germ cell survival and fertility through specialized cells like granulosa and Theca cells (ovaries), and Sertoli and Leydig cells (testis), that are nurturing the germ cells and provide germ cell survival signals such as Dmrt1 [14]. Thus, blocking the function of proteins located in these nurturing cells or in the germ cells could potentially lead to sterility.

To be able to efficiently target proteins needed for germ cell survival or fertility, it is necessary, unless applying conditional knockout techniques, to target those genes that have an exclusive function limited to the gonad of the fish. As for now, some information is available on genes exclusively expressed in germ cells in Atlantic salmon [7,8,9], and only one study has so far revealed a function of such proteins in salmon [3]. Limited information is available for genes expressed exclusively in the somatic gonad of salmon. The most known is the sex determining gene sdy, which is expressed in the male somatic gonad [15, 16]. It is also known that the gonadotropin receptors follicle-stimulating hormone receptor (fshr) and luteinizing hormone receptor (lhcgr) are predominantly expressed in the gonads of salmon, and in females the expression is restricted to granulosa and Theca cells [17]. However, a weak expression of fshr and lhcgr have also been found in extra-gonadal tissues like gills, brain, liver and heart [18]. The nuclear progesterone receptor (pgr) is mainly expressed in testis tissue, but also ovary and weakly in pituitary and spleen. Within the testis, pgr transcripts are restricted to Sertoli cells [19]. Aromatase (Cyp19a1a) has been located to germ and somatic cells in testis tissue from one year old males [20]. Furthermore, cyp19a1a transcripts have been detected in GCF and WT ovaries, while not detected in 7 other adult tissues. A similar expression profile has been shown for anti-müllarian hormone (amh) [3]. The same study reported expression of forkhead box L2 (foxl2) and sex-determining region Y-box 9a (sox9a) in both GCF and WT ovary and testis tissue, however von Schalburg et al. [21] have shown that both foxl2 and sox9a are also expressed in a number of extragonadal adult tissues. Additional genes that are associated with gonadal functions, such as for example insulin-like growth factor 3 (igf3), have also been detected in both GCF and WT gonads of salmon [22]. Nevertheless, information is currently lacking on potential extra-gonadal expression and additional functions of such genes.

The GCF Atlantic salmon model represents a unique opportunity to identify genes that are exclusively expressed in gonadal somatic cells in this species. The GCF salmon male model contains a high number of Sertoli cells [3], which makes it likely to identify Sertoli cell specific genes. To identify unique somatic and germ cell factors, we performed transcriptome sequencing of gonad tissue (testis) from male wild type (WT) and GCF salmon. Our dataset was then compared with available multi-tissue transcriptome data (GenBank GBRB00000000.1 [23];) to eliminate genes with extra-gonadal expression. This study therefore presents more germ cell specific genes, which adds to the current group of known germ cell specific genes in salmon and opens for further research on potential sterility targets. Furthermore, we have provided a list of gonadal somatic genes and characterized the expression patterns of the two most highly expressed gonadal somatic genes in salmon, gsdf and inha.

Results

Gonad specific genes preferentially expressed in gonadal somatic cells

In silico filtering criteria applied in this study were based on a previous similar study [9] and are discussed below. To first identify genes expressed specifically in salmon gonads we searched available multi-tissue transcriptome sequencing data (GenBank GBRB00000000.1 [23];) for genes with 50 or less reads in extra-gonadal tissues. Further, genes with less than 100 reads in testis or ovary were excluded from the analysis. According to these criteria, 389 unique gene ID’s were identified (Additional file 1). Several genes such as deleted in azoospermia like (dazl) (GenBank 106,588,867), dead end (dnd) (GenBank 101,448,053) and piwi like RNA-mediated gene silencing 1 (piwil1) (GenBank 106,585,526), well-known to be expressed preferentially in gonads, were identified in this list (Additional file 1).

Next, the 389 identified sequences were merged with the sequences obtained from GCF and WT testis to reveal which genes that were expressed in testicular somatic cells. Eleven genes had 100 or more reads in both GCF and WT testis (Fig. 1; Table 1). Of these, two genes and one noncoding RNA have not been characterized. Of the known genes, solute carrier family 25 member 12-like (slc25a12l) and cathepsin s-like (ctssl) had the lowest numbers of reads (between ~ 200–600 reads). Another cathepsin – cathepsin L (ctsl) – had a noteworthy higher expression with ~ 12,000–22,000 reads. The remaining genes – two bone morphogenetic protein 6-like (bmp6l) genes, nodal, protein inhibin alpha chain (inha) and gonadal somatic-derived factor (gsdf) – which all belong to the TGF-β superfamily, had highly variable expression levels ranging from ~ 1000–2000 reads (bmp6l) and ~ 2000–8000 reads (nodal) to more than 22,000 reads (inha and gsdf). Another noteworthy observation is the clear difference in number of transcripts of nodal (~ 3-fold increase) and gsdf (~ 3,5-fold decrease) from WT to GCF testis. The two genes with the highest expression in testicular somatic cells (and therefore potentially have a high activity in these cell types), inha and gsdf, were selected for further characterization in salmon males and females.

Fig. 1
figure 1

Expression profiles of 11 gonadal somatic genes. Expression profiles, shown as number of reads, of 11 genes with expression confined to gonadal somatic cells in adult immature Atlantic salmon testis tissue. WT; wild type testis (n = 3), GCF; germ cell-free testis (n = 4)

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Table 1 Gonad specific genes with expression in testicular somatic cells of Atlantic salmon
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Gonad specific genes preferentially expressed within germ cells

To identify genes that are preferentially expressed in salmon germ cells, the gonad specific 389 genes were filtered again based on number of reads in WT and GCF testis; genes with more than 50 reads in the GCF group, and transcripts with less than 100 reads in the WT group, were excluded. Based on these criteria, 194 genes were expressed in WT testis and not GCF testis (Fig. 2). Several well-known germ cell-specific genes like piwil1 (GenBank 106,585,526), dazl (GenBank 106,588,867) and tudor domain containing 6 (tdrd6) (GenBank 106,608,113) could be found in this list of 194 genes [Fig. 2; (Additional file 2)]. Among these, 69 genes were annotated with KEGG pathway ID’s (when removing human diseases). The pathway with the highest number (14) of genes was lysine degradation, followed by complement and coagulation cascades (13 genes). The pathways linked to more than one gene is shown in Table 2.

Fig. 2
figure 2

Expression profiles of 194 germ cell specific genes. Expression profiles, shown as number of reads, of 194 genes with expression confined to germ cells in adult immature Atlantic salmon testis tissue. WT; wild type testis (n = 3), GCF; germ cell-free testis (n = 4)

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Table 2 KEGG pathways linked to gonad specific genes preferentially expressed in germ cells
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Phylogenetic analysis of the Inha protein sequence and chromosomal synteny of the inha gene

To confirm the identity of the two selected genes dsl1 (gsdf, see below) and inha (GenBank 106,568,506 and 106,575,603) in this study, a phylogenetic analysis and a search for chromosomal synteny was performed. However, in the case of the gene annotated to dsl1l (GenBank 106,568,506), a homology search had previously been performed using EST databases (GenBank CK897686.1 for salmon is identical to GenBank 106,568,506), suggesting that this gene is gonadal somatic-derived factor (gsdf) [24]. The same gene has also been studied in salmon by Lubieniecki et al. [25].

In the case of inha, a phylogenetic analysis (Additional file 4) was performed by comparing Inha protein sequences from tetrapods, teleosts and Spotted gar, a ray-finned fish that diverged from the teleosts [26]. To further confirm the identity of inha, we investigated the synteny of chromosomal regions associated with this gene in a selection of fish species. We searched for genes located up- and downstream of inha, and conserved genes were identified in all the selected species (Fig. 3). In Atlantic salmon, 3 additional inha-like genes were found, however they are annotated as, and appear to be, pseudo genes (GenBank Accession no 106574595; 106,574,698; 106,596,067).

Fig. 3
figure 3

Synteny of chromosomal regions associated with inha. Overview of the 5 most adjacent up-and downstream genes to inha (a; gene symbols, b; gene IDs) in a selection of fish species, including Atlantic salmon, is shown. Genes marked with dark grey color are conserved in salmon and the other species. Genes marked with light grey color are conserved in other species than salmon. Chr; Chromosome

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Expression of gsdf and inha in Atlantic salmon tissues

A qPCR tissue screen was performed to confirm that gsdf and inha are specifically expressed in gonads of Atlantic salmon. As shown in Fig. 4, gsdf (A) and inha transcripts (B) are abundant in ovary and testis, and very low or undetected in all other tissues examined. Although the statistical test applied detected significant differences between groups, the post-test was not able to distinguish which groups that were significantly different; 3 biological replicates were used per tissue.

Fig. 4
figure 4

Relative expression of gsdf and inha – tissue distribution in adult immature salmon. Expression of gsdf a and inha b relative to EF1α, in different tissues from immature adult Atlantic salmon, measured by qPCR. All values were calibrated to the average ΔCt of the group with the highest expression (testis). The data are shown as mean with SEM (N = 2–3 individuals). ND, not detected

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Transcript levels of gsdf and inha were further measured in GCF and WT ovary and testis. As shown in Fig. 5, gsdf and inha transcripts were detected in both GCF and WT ovary (A, C) and testis (B, D). Further, we observed that gsdf expression was higher in WT than GCF ovary tissue, and that the expression remained unchanged from the immature to the early vitellogenic group (Fig. 5a). In testis tissue, gsdf was downregulated from immature to mature samples (Fig. 5b). Expression of inha was upregulated in early vitellogenic ovary and mature testis (Fig. 5c, d).

Fig. 5
figure 5

Relative expression of gsdf and inha in germ cell-free and wild type gonads. Expression of gsdf a, b and inha c, d relative to EF1α, in GCF and two stages of WT ovary a, c and testis b, d of Atlantic salmon, measured by qPCR. All values were calibrated to the average ΔCt of the immature WT group. The data are shown as mean with SEM (N = 9–24 individuals). Different letters represent significant differences between groups (p < 0.05). GCF, germ cell-free; WT, wild type

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To investigate the gonadal location of gsdf and inha transcripts in salmon, in situ hybridization (ISH) was performed on both ovary and testis tissue. In females, gsdf and inha transcripts were detected in granulosa cells (Fig. 6b, d), which was also confirmed in females with larger, vitellogenic oocytes (Fig. 6g, i). In the case of inha, we also observed some staining within the ooplasm of immature oocytes (Fig. 6d), however, a similar staining was present in immature oocytes where the sense probe was applied (Fig. 6e) and is therefore considered as unspecific staining. In males, gsdf and inha were expressed in Sertoli cells (Fig. 6l, n).

Fig. 6
figure 6

Gene expression of gsdf and inha – localized to granulosa and Sertoli cells. Expression of gsdf and inha in Atlantic salmon ovary (gsdf: b, c, g, h; inha: d, e, i, j) and testis (gsdf: l, m; inha: n, o), visualized by in situ hybridization. Germ cell and gonadal somatic cell structures are shown by Toluidine Blue stained plastic sections of immature ovary a and testis tissue k, and a Hematoxylin Eosin Saffron stained paraffin section of a vitellogenic oocyte f. Expression of gsdf and inha is represented by the staining in images where the antisense (AS) probe was applied. b-e Immature ovary. g-j Vitellogenic ovary. l-o Immature testis. Gc, granulosa cells; op, ooplasm; tc, theca cells; zr, zona radiata; Sc, Sertoli cell; n, nucleus of spermatogonia; nu, nucleoli of spermatogonia; S, sense probe

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Discussion

In this study we have made available transcriptome data on genes expressed in the germ cell-free (GCF) Atlantic salmon testis tissue, which can also be compared to genes that are expressed in the same type of tissue with germ cells present. We have from these data identified genes that are preferentially expressed in both gonadal somatic cells (Fig. 1; Table 1) and germ cells of salmon [Fig. 2; (Additional file 2)], which adds novel factors to the list of genes that have been reported previously [7,8,9]. This dataset therefore provides valuable information for further studies on potential sterility targets in Atlantic salmon, but also serves as an interesting resource for the field of reproductive biology in a wider context, such as cell-cell communication within the testis.

Validation of tissue specificity of the identified transcripts

In this study we characterized in more detail two genes with a high expression (read counts) in gonadal somatic cells, gsdf and inha. QPCR analysis showed that both these genes are expressed in ovary and testis tissue lacking germ cells, confirming their expression in gonadal somatic cells. ISH analysis showed that gsdf and inha transcripts are localized to granulosa cells in females and in Sertoli cells in males, confirming their specific gonadal somatic expression. A similar expression pattern has recently been shown in salmon testis tissue for another gonadal somatic gene, anti-müllarian hormone (amh) [27], which supports our findings. In the case of germ cell-specific genes identified in this study, several of these including piwil1, dazl, la-related protein 6-like (larp6l), bone morphogenetic protein 15-like (bmp15l) and folliculogenesis specific bHLH transcription factor (figla) have been validated by us using qPCR and ISH in previous publications [8, 9].

In silico filtering thresholds

To be able to say that a gene is expressed or not in a given tissue sample, a threshold for number of reads needs to be determined. In this study we applied the same thresholds as a previous similar study [9]; firstly, genes with 50 or less reads are not considered expressed, while fewer reads are considered as background/noise. This choice is supported by the fact that for example vasa, known to be expressed exclusively in germ cells, still had 70 and 57 reads in eye and gill tissues. Secondly, we chose 100 reads as a minimum for genes considered to be expressed; this creates a clear distinction from genes considered not expressed (50 or less reads). When applying these criteria for in silico filtering, several well-known 1) gonad-specific genes were identified in the list of gonad-specific genes [Additional File 1], 2) well-known germ cell specific and gonadal somatic specific genes were identified in the lists of genes expressed in germ cells [Additional File 2] and in gonadal somatic cells (Table 1), which validates the chosen thresholds. Nevertheless, cutoffs most likely also cause some relevant genes to be excluded from the analysis.

Genes with expression preferentially in gonadal somatic cells

To our knowledge, this is the first study to screen for genes with expression exclusively in gonadal somatic cells in Atlantic salmon, using a knockout model. Only 11 genes (gsdf, inha, ctsl, nodal, two bmp6l, ctssl, slc25a12l, two unknown genes and one unknown ncRNA) had 100 or more reads in GCF and WT testis (Table 1) while at the same time being exclusive to gonadal tissue. The most striking finding was that around half of these genes, (gsdf, inha, nodal and the two bmp6l) are associated with the TGF-β pathway. They also represented the two genes with the highest expression in testicular somatic cells, gsdf and inha, two genes that we have studied and discussed in more detail in the next sections. TGF-β proteins are all secreted ligands known to be essential for many processes in gonad development including diverse functions in the testis associated with germ cells, Sertoli cells, growth and fertility [28]. However in our case, the finding of TGF-β transcripts restricted to gonadal somatic cells is truly promising for future targeting since TGF-β proteins are secreted proteins that may likely have functions confined to the gonad, which is a prerequisite for 1) functional studies of the gonad without using conditional mutants, and 2) finding potential target proteins for a sterility vaccine in salmon. Interestingly, bmp6 plays a role in human Sertoli cell proliferation and apoptosis [29]. In fish, Bmp6 has been linked to fin regeneration, iron metabolism, tooth patterning and viability and growth [30,31,32,33]. Furthermore, bmp6 has been linked to ovarian function in tounge sole and zebrafish [34, 35]. The diverse roles of this protein may be explained by its widespread expression in many tissues, however our finding of a unique bmp6-like transcript confined largely to the somatic gonad may be a result of sub-functionalization and a specialized function of this protein in the gonad of salmon. Nodal, another TGF-β protein, is involved in various processes within mammalian testes. More specifically, Nodal signaling plays a role in regulation of pluripotency factor expression, proliferation and survival of germ cells, as well as establishment of the somatic niche through seminiferous cord formation, steroidogenesis and Sertoli cell function (reviewed by [36]). In human testis, Nodal has been shown to regulate Sertoli cell proliferation [37]. In zebrafish Nodal is involved in dorso-ventral patterning of the embryo [38], while to our knowledge no specific studies have investigated the function of this protein in the fish gonad. A high nodal expression restricted to gonads in salmon as observed in this study suggests a role of this gene in reproduction in fish. Interestingly, we observed a 3-fold higher nodal expression in salmon testis lacking germ cells, compared to WT testis. This may indicate that the presence of germ cells has an inhibitory effect on nodal expression in Sertoli cells of immature testis tissue, and that Nodal is part of the germ-somatic cell communication in Atlantic salmon. Two Cathepsins, Ctsl and Ctssl, had gonad specific transcripts and were also expressed within testicular somatic cells in salmon. These are Cysteine Cathepsins, proteases involved in a number of physiological processes like protein breakdown and immune responses [39, 40]. Interestingly, the stage-specific expression of ctsl in rat Sertoli cells is influenced by the presence or absence of germ cells, suggesting a role of ctsl in germ-somatic cell interaction in testis tissue [41]. A higher number of ctsl as well as ctssl reads in GCF compared to WT testis tissue as observed in this study, may suggest possible involvement for these Cathepsins in germ-somatic cell signaling in salmon.

Gsdf

The identity of Atlantic salmon gsdf has previously been shown by phylogenetic analysis [24]. In this study we found that gsdf is highly expressed in females and males, with specific transcript localization in granulosa and Sertoli cells, respectively. Likewise, expression of gsdf in the same cell types has been observed in several fish species including rainbow trout (Oncorhynchus mykiss), medaka (Oryzias latipes), zebrafish, Nile tilapia (Oreochromis niloticus), Olive flounder (Paralichthys olivaceus), Spotted scat (Scatophagus argus) and Japanese flounder (Paralichthys olivaceus) [24, 42,43,44,45,46,47,48]. Transcripts of gsdf have also been detected within oocytes in Olive flounder, and within spermatogonia and spermatids in Chinese tongue sole (Cynoglossus semilaevis) [46, 49]. Although the function(s) of gsdf in male fish seems strongly associated with germ cells, it may differ between species since this gene has been linked to several processes such as proliferation of primordial germ cells and spermatogonia in rainbow trout, sex determination in Oryzias luzonensis, sex differentiation in Chinese tounge sole, and testis differentiation in medaka and Nile tilapia [24, 49,50,51,52,53]. Although we do not know the function of Gsdf in salmon testis, it is likely that this protein has one or more roles as described in other fish species. One possible role may be associated with testis maturation, since we observed that gsdf expression was higher in immature compared to mature testis, similar to what has been observed in medaka, Japanese flounder, wrasse (Halichoeres trimaculatus) and rice field eel (Monopterus albus) [43, 48, 54, 55]. The fact that we detected a 3.5-fold lower number of gsdf reads in testis tissue devoid of germ cells compared to intact testis tissue may suggest a role for gsdf in the communication between germ cells and testicular somatic cells in Atlantic salmon.

In salmon females we observed gsdf expression specifically localized to granulosa cells in both previtellogenic and vitellogenic follicles. The localization of gsdf expression in ovarian somatic cells was further confirmed by the presence of gsdf transcripts in GCF ovaries, although at a lower level compared to normal ovaries. Limited information exists on the role of Gsdf in females. It is known that targeted disruption of gsdf in medaka causes abnormal folliculogenesis associated with sterility [56]. In zebrafish, Gsdf may have a role in regulating ovarian follicle maturation and expression of genes involved in steroid biosynthesis, obesity, diabetes, and female fertility [57]. Lack of Gsdf in medaka has been linked to a dysregulation of oocyte development [58]. In coho salmon (Oncorhynchus kisutch) ovarian tissue, gsdf transcript levels are significantly increased during early secondary growth. The authors speculated that Gsdf may be involved in granulosa cell proliferation [59]. In contrast, we did not observe any increase in gsdf expression in early vitellogenic salmon ovaries. This may be explained by a difference in the maturation stage, since the rainbow trout ovaries in the previous study were more advanced than the salmon ovaries applied in the current study. Based on our and others’ findings, we speculate that salmon gsdf, due to its specific localization in granulosa cells, may be involved in folliculogenesis.

Inha

In this study, Atlantic salmon inha was confirmed by chromosomal synteny to other fish species and by its close phylogenetic relationship within teleosts. Furthermore, we confirmed that inha expression is localized in granulosa and Sertoli cells of salmon ovary and testis, respectively. In agreement with our observations, studies in rainbow trout and zebrafish females have shown that inha expression is restricted to the somatic follicle cells surrounding the oocytes [60, 61]. Similarly, male rainbow trout inha expression is localized to Sertoli cells, although inha transcripts were also detected in Leydig cells [60]. In the current study it was difficult to distinguish Leydig cells from the clearly stained and numerous Sertoli cells; thus, a conclusion could not be made on whether the Leydig cells contained inha transcripts. Based on the similarities of our results to the above-mentioned studies we suggest a similar functional role for Inha in salmonids.

Further characterization of inha in salmon revealed that the transcript level increased during puberty in both females and males, suggesting a role in sexual maturation. In the case of females, this is supported by studies where inha expression increased during folliculogenesis and peaked during final oocyte maturation in zebrafish [61] and coho salmon [62]. Limited information exists on the function of Inha in teleost testis, however some insight has been generated from studies in rainbow trout. It was shown that testicular inha expression is upregulated by Fsh and Lh [63], and that the response in inha transcript levels to Fsh is mediated through the production of sex steroids [64]. This interaction of inha transcript levels with Fsh and sex steroids in trout, together with the localization of inha mRNA in Sertoli cells and increase of testicular inha expression during spermatogenesis (this study), suggest an essential role of Inha in spermatogenesis in salmonids.

Genes with expression preferentially in germ cells

In our dataset we also had the opportunity to identify transcripts confined to germ cells, based on their expression in WT and absence in GCF testis. We cannot rule out however, that some of these genes may be expressed in gonadal somatic cells, but are downregulated as a response to the absence of germ cells. Nevertheless, numerous candidates were identified [Fig. 2; (Additional file 2)], including several transcripts encoding well-known germ cell specific proteins such as Piwi, Dazl and Tdrd6. KEGG pathways annotated to the genes revealed that several of them are involved in lysine degradation and complement and coagulation cascades (Table 2). Lysine degradation is a pathway essential for metabolic function in the cell and can in this way have a special function in germ cells. Another speculation of an enrichment of these pathways in the gonad containing germ cells may be a potentially higher content of blood vessels in gonads with germ cells, since these pathways are associated with blood activity.

Conclusions

This study provides transcriptome data from salmon testis tissue with and without germ cells. By comparing these transcriptomes, we identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to the somatic part of the gonad. Five of these 11 genes (gsdf, inha, nodal and two bmp6-like genes) encode TGF-β proteins. Among the gonadal somatic genes, gsdf and inha had the highest numbers of transcripts. Expression of gsdf and inha was restricted to ovarian granulosa and testicular Sertoli cells. While inha transcript levels increase during puberty in both females and males, expression of gsdf decreases from immature to mature males. Information on genes with functions in gonadal somatic cells will be useful in future studies with the aim to elucidate how germ cells are supported for development and survival. Ultimately, knowledge on how germ cells develop and stay alive may lead to the possibility to control gametogenesis in this commercially important species.

Methods

Animals, rearing and sampling of tissue

The use of the experimental animals in this study was performed in strict accordance with the Norwegian Animal Welfare Act of 19th of June 2009, in force from 1st of January 2010. All year classes of fish reared were approved by the Norwegian Animal Research Authority (http://www.fdu.no/fdu/NARA, permit number 5741. A completed ARRIVE guidelines checklist can be viewed in [Additional File 5]. All the fish were reared and sampled at Matre Aquaculture Research Station, Matredal, Norway. Four groups of fish were applied, of which groups 1–3 have been published in previous studies; consequently, only 3 experimental animals were sacrificed solely for the current study (group 4).

Group 1: 7 Atlantic salmon males, 1 year old, reared and sampled as described previously [3]. The following tissues were included for RNA sequencing to screen for genes expressed in gonadal somatic cells: GCF/dnd-knockout (n = 4) and WT testis (n = 3).

Group 2: 6 Atlantic salmon (3 females, 3 males), 1–2 years old, reared and sampled as described previously [9]. The following tissues were included for a qPCR analysis to screen for inha and gsdf expression in various parts of the body: ovary, testis, brain, eye, muscle, fin, heart, gill, spleen, gut, liver, head kidney, skin (Fig. 4).

Group 3: 83 Atlantic salmon (24 GCF/dnd-knockout, 10 immature and 9 early vitellogenic females; 22 GCF/dnd-knockout, 9 immature and 9 mature males), 2 years old, reared and sampled as described previously [22]. The following tissues were included for qPCR analysis to reveal if inha and gsdf expression in the gonads change through puberty: GCF, immature and early vitellogenic ovary, and GCF, immature and mature testis (Fig. 5). The selected gonadal stages have been described previously based on gonado-somatic index, plasma levels of sex steroids and histology [22].

Group 4: 3 Atlantic salmon (1 immature and 1 vitellogenic female; 1 immature male), reared in indoor tanks under standard rearing conditions and a maturation inducing regime [65]. The fish were fed ad libitum with a standard commercial diet. Prior to sampling, all fish were anesthetized with 2 ml/L finquel vet, and sacrificed by cutting into the medulla oblongata, the connection between the spinal cord and the skull. Gonad tissue was collected and fixed in 4% paraformaldehyde in PBS over-night at 4 °C. Subsequently, the samples were washed 2 × 2 hours in PBS, immersed in 25% sucrose in PBS, and stored over-night at 4 °C. Finally, the samples were embedded in Tissue Tek and stored in − 80 °C until ISH. The following tissues were included for ISH to reveal the cellular localization of gsdf and inha transcripts in the gonads: immature testis and ovary, and vitellogenic ovary (Fig. 6).

In silico analysis and RNA sequencing

Firstly, to identify genes expressed specifically in salmon gonads, we screened available salmon multi-tissue (ovary, testis, brain, eye, gill, gut, head kidney, heart, liver, muscle, nose, ovary, pyloric caecum, skin, and spleen) transcriptome sequencing data (GenBank GBRB00000000.1 [23];). Genes with expression in extra-gonadal tissues were removed from the analysis.

Secondly, to identify which of the gonad specific genes that were expressed in the somatic part of the gonads, and which of them that were expressed within germ cells, we sequenced total RNA from 3 WT and 4 GCF testis (raw sequence reads accession no. PRJNA550414; https://www.ncbi.nlm.nih.gov/bioproject/PRJNA550414) using the HiSeq.2000 sequencing platform (Illumina). RNA-seq paired end sequences were mapped with Bowtie2 against the gene model transcripts of Atlantic salmon genome (ICSASG_v2) with standard Bowtie2 parameters [66]. Raw count table for each gene was extracted using SAMtools idxstats [67]. The read counts were normalized to the total reads in the sample with the smallest number of reads. A summary of the mapping can be viewed in [Additional File 6]. KEGG pathway analysis was performed by mapping the KEGG annotated genes to KEGG pathways as described in the KEGG Mapper tool [68]. Consequently, germ cell specific genes could be identified due to lack of expression in the GCF group, while gonadal somatic genes could be identified due to expression in both the GCF and WT group.

Phylogeny and synteny

Phylogeny and chromosomal synteny were investigated for inha to confirm the identity of this gene. For the phylogenetic analysis, Inha protein sequences from the following species were retrieved from https://www.ncbi.nlm.nih.gov/: Atlantic salmon (GenBank XP_014007683.1), rainbow trout (GenBank XP_021466674.1 (1) and NP_001117672.1 (2)), zebrafish (Danio rerio) (GenBank NP_001038669.1), Japanese medaka (Oryzias latipes) (GenBank XP_020564073.1), Common carp (Cyprinus carpio) (GenBank XP_018966331.1), Ballan wrasse (Labrus bergylta) (GenBank XP_020497817.1), Atlantic herring (Clupea harengus) (GenBank XP_012676518.1), Spotted gar (Lepisosteus oculatus) (GenBank XP_015214647.1), Chicken (Gallus gallus) (GenBank NP_001026428.1) and Xenopus (GenBank NP_001027522.1 (1), OCT63342.1 (2) and AAI70257.1 (3)). The sequences were imported into MEGA version X [69], and sequence alignments were performed using MUSCLE (Multiple Sequence Comparison by Log-expectation). All positions containing gaps and missing data were eliminated. The phylogenetic analysis was performed applying the Neighbor-Joining method [70]. A bootstrap test [71] was used to test the reliability of the inferred trees.

For the chromosomal synteny, BLAST searches for orthologous genes for salmon inha (GenBank 106,575,603) were performed at https://www.ncbi.nlm.nih.gov/. The following fish species were included in the analysis: rainbow trout (Genbank 110,528,842 and 100,135,804), zebrafish (GenBank 570,520), Japanese medaka (GenBank 101,157,430) and Ballan wrasse (GenBank 109,990,152). The Atlantic salmon sequences and annotation were obtained from the official genome annotation (NCBI Salmo salar Annotation Release 100).

RNA extraction, cDNA synthesis and real-time, quantitative PCR

For the group 1 samples, RNA extraction was performed using the MiRNeasy Mini kit (Qiagen), according to the manufacturer’s instructions. Up to 50 mg tissue was used for each sample, and the extracted RNA had absorbance ratios 260/280 of 1,9–2,1 (NanoDrop Spectrophotometer/ThermoFisher Scientifics), and RNA intergrity numbers 7,6–9 (Bioanalyzer/Agilent Technologies). For the group 2 and 3 samples, RNA was extracted and DNase-treated as described previously ([9, 22], respectively). cDNA was synthesized from 125 ng RNA (group 2 samples) and 500 ng RNA (group 3 samples) using the Superscript VILO cDNA synthesis kit (Invitrogen), according to the manufacturer’s instructions. Primers and probe sequences for inha and gsdf were designed online (https://www.genscript.com/ssl-bin/app/primer (Genscript®)), and can be seen in Table 3. Primers and probe sequences for the housekeeping gene elongation factor 1-alpha (ef1α) were published previously [72]. QPCR was performed in duplicates in 384-well optical plates in a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) (all group 3 samples, and group 2 samples for gsdf) or SDS 7900HT Fast Real-Time PCR system (Applied Biosystems) (group 2 samples for inha) using default settings. One μl cDNA was used in a 5 (gsdf) or 10 μl (inha) Fast Taqman qPCR reaction (ThermoFisher Scientific). No-template controls for each gene were run in all qPCR plates. The relative gene expression level was calculated using the comparative Ct (or 2−ΔΔCt) method. All values were normalized to ef1a and calibrated to the average ΔCt of the testis tissue (group 2 samples) or the immature WT gonads (group 3 samples).

Table 3 Primer and probe sequences
Full size table

In situ hybridization

A PCR product was generated using gsdf or inha gene-specific primers (Table 3) for the synthesis of cRNA-probes for ISH. The PCR products were sequenced following a PCR cleanup using illustra ExoProStar 1-Step (GE Healthcare Life Sciences), according to the manufacturer’s instructions. The returned sequences were blasted in the NCBI database (https://www.ncbi.nlm.nih.gov/) against several species, confirming that the primers had amplified the gsdf and inha genes. ISH cRNA antisense and sense probes were synthesized from 1 μg PCR product applying primers containing Sp6 or T7 sequence, respectively (Table 3), together with a digoxigenin-alkaline phosphatase (DIG-AP) RNA Labeling Kit (SP6/T7) (Roche Diagnostics). The probes were precipitated, washed, and resuspended in MilliQ water as described by Weltzien et al. [73]. Probe size and quality were checked with a Bioanalyzer (Agilent Technologies), and the DIG incorporation in the probes was inspected by performing a spot-test. ISH by DIG-AP was performed as described by Weltzien et al. [73].

Statistics

Statistical tests were performed using GraphPad Prism 7.02 (GraphPad Software Inc.). All qPCR datasets were tested for normal distribution using a D’Agostino & Pearson omnibus normality test. For datasets with no normal distribution, or too few n to test for normal distribution, non-parametric tests were performed to calculate differences between groups. A Kruskal-Wallis with Dunn’s multiple comparisons post test was applied for inha in different stages of ovary tissue (Fig. 5c), and expression of gsdf and inha in multi tissues of adult immature salmon (Fig. 4). An ordinary one-way ANOVA with Tukey’s multiple comparisons post test was applied for gsdf and inha expression in different stages of testis tissue (Fig. 5b, d), and gsdf in ovary (Fig. 5a).

Availability of data and materials

The raw RNA sequencing data are available at https://www.ncbi.nlm.nih.gov/bioproject/PRJNA550414. The gene lists resulting from filtering of RNA sequencing data are available in the published paper and its additional files.

Abbreviations

GCF:

Germ cell-free

ISH:

In situ hybridization

TGF-β:

Transforming Growth Factor

WT:

Wild type

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Acknowledgements

We would like to thank Ivar Helge Matre and Lise Dyrhovden for rearing of the fish and assistance during samplings.

Funding

This study was funded by the Norwegian Research Council (NRC) BIOTEK2021 project SALMOSTERILE (221648). NRC provided funding for author salaries, but did not have any additional role in this study.

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  1. Institute of Marine Research, P.O. Box 1870, Nordnes, NO-5817, Bergen, Norway

    Lene Kleppe, Rolf Brudvik Edvardsen, Tomasz Furmanek, Eva Andersson, Kai Ove Skaftnesmo & Anna Wargelius

  2. Cflow Fish Handling AS, Holsneset 25, N-6030, Langevåg, Norway

    Frida Thyri Segafredo

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Experimental design: AW, RBE, EA, LK. Performance of the experiments: LK, RBE, TF, EA, KOS, FTS, AW. Data analysis: LK, TF, FTS, AW. Writing of the paper: LK, AW. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Lene Kleppe.

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Ethics approval

The use of these animals was performed in strict accordance with the Norwegian Animal Welfare Act of 19th of June 2009, in force from 1st of January 2010. All year classes of fish reared were approved by the Norwegian Animal Research Authority (http://www.fdu.no/fdu/NARA, permit number 5741.

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Not applicable.

Competing interests

The authors declare that they have no competing interests.

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Supplementary information

Additional file 1.

Gonad specific genes. List of gonad specific (≤50 reads in extragonadal tissues and ≥ 100 reads in ovary and testis) genes in adult Atlantic salmon. Transcript ID, Gene ID, number of reads in multiple tissues (brain, eye, gill, gut, head kidney, heart, kidney, liver, muscle, nose, pyloric caecum, skin, spleen, ovary, testis (GenBank GBRB00000000.1 [23];) are shown

Additional file 2.

Gonad specific genes with expression preferentially within germ cells. List of gonad specific (≤50 reads in extragonadal tissues and ≥ 100 reads in ovary and testis) genes with expression preferentially within germ cells (≤50 reads in germ cell-free testis and ≥ 100 reads in WT testis) of adult Atlantic salmon males. Transcript ID, Gene ID, number of reads in multiple tissues (brain, eye, gill, gut, head kidney, heart, kidney, liver, muscle, nose, pyloric caecum, skin, spleen, ovary, testis (GenBank GBRB00000000.1 [23]; (highlighted in grey)), and number of reads in juvenile wild type (WT) and dead end knockout (dnd-KO) testis tissue (highlighted in green) are shown

Additional file 3.

KEGG pathways. Full list of KEGG pathways annotated to gonad specific genes with expression preferentially within germ cells [Additional File 2] of adult Atlantic salmon males. Pathway ID, pathway, number of genes within each pathway, gene ID and symbol are shown

Additional file 4.

Phylogenetic analysis of Inha protein sequences. Phylogenetic analysis of Inha protein sequences from Atlantic salmon (GenBank XP_014007683.1), rainbow trout (GenBank XP_021466674.1 (1) and NP_001117672.1 (2)), zebrafish (Danio rerio) (GenBank NP_001038669.1), Japanese medaka (Oryzias latipes) (GenBank XP_020564073.1), Common carp (Cyprinus carpio) (GenBank XP_018966331.1), Ballan wrasse (Labrus bergylta) (GenBank XP_020497817.1), Atlantic herring (Clupea harengus) (GenBank XP_012676518.1), Spotted gar (Lepisosteus oculatus) (GenBank XP_015214647.1), Chicken (Gallus gallus) (GenBank NP_001026428.1) and Xenopus (GenBank NP_001027522.1 (1), OCT63342.1 (2) and AAI70257.1 (3)). The evolutionary history was inferred using the Neighbor-Joining method [70]. The bootstrap consensus tree inferred from 500 replicates is taken to represent the evolutionary history of the taxa analyzed [71]. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test are shown next to the branches [71]. The evolutionary distances were computed using the Poisson correction method [74] and are in the units of the number of amino acid substitutions per site. This analysis involved 13 amino acid sequences. All ambiguous positions were removed for each sequence pair (pairwise deletion option). A total of 432 positions were in the final dataset. Evolutionary analyses were conducted in MEGA X [69]

Additional file 5.

ARRIVE Guidelines Checklist. Completed “The ARRIVE Guidelines Checklist” for reporting information on experimental animals in this study

Additional file 6.

RNA seq mapping summary. Summary of the mapping of RNA-seq paired end sequences against the gene model transcripts of the Atlantic salmon genome (ICSASG_v2). Sample name, total reads, mapped reads and % mapping is shown

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Kleppe, L., Edvardsen, R.B., Furmanek, T. et al. Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon. BMC Genomics 21, 99 (2020). https://doi.org/10.1186/s12864-020-6513-4

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Keywords

BMC Genomics

ISSN: 1471-2164

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