Analysis of Gene expression in soybean (Glycine max) roots in response to the root knot nematode Meloidogyne incognita using microarrays and KEGG pathways
BMC Genomics volume 12, Article number: 220 (2011)
Root-knot nematodes are sedentary endoparasites that can infect more than 3000 plant species. Root-knot nematodes cause an estimated $100 billion annual loss worldwide. For successful establishment of the root-knot nematode in its host plant, it causes dramatic morphological and physiological changes in plant cells. The expression of some plant genes is altered by the nematode as it establishes its feeding site.
We examined the expression of soybean (Glycine max) genes in galls formed in roots by the root-knot nematode, Meloidogyne incognita, 12 days and 10 weeks after infection to understand the effects of infection of roots by M. incognita. Gene expression was monitored using the Affymetrix Soybean GeneChip containing 37,500 G. max probe sets. Gene expression patterns were integrated with biochemical pathways from the Kyoto Encyclopedia of Genes and Genomes using PAICE software. Genes encoding enzymes involved in carbohydrate and cell wall metabolism, cell cycle control and plant defense were altered.
A number of different soybean genes were identified that were differentially expressed which provided insights into the interaction between M. incognita and soybean and into the formation and maintenance of giant cells. Some of these genes may be candidates for broadening plants resistance to root-knot nematode through over-expression or silencing and require further examination.
Plant parasitic nematodes cause about US $100 billion in crop losses annually [1, 2]. Root-knot nematodes (RKN; Meloidogyne spp.) are sedentary endoparasites. The most economically important species are Meloidogyne incognita and M. arenaria. Both are widespread and are considered as major crop pathogens worldwide. The RKN can be easily recognized by the "knots" or "galls" that form where they feed on roots [3, 4]. These nematodes cause dramatic morphological and physiological changes in plant cells. Some plant genes are subverted by nematodes to establish feeding cells, and transcripts of several nematode genes were identified during infection . Root-knot nematode damage to soybean (Glycine max) can be severe, especially when fields previously planted in cotton are rotated into soybean .
The RKN life cycle is complex [for review see: [3–5, 7]]. The egg is laid in the soil or in plant tissues. The first stage juvenile develops inside the egg and molts one time to the second-stage juvenile (J2). When the J2 hatches from the egg, it infects the root close to the root tip in the elongation zone and migrates to the vascular tissue, where it establishes a feeding site by injecting esophageal proteins into several plant cells and it recruits host genes to alter the morphology of the host cells. Host cells become binucleate and then undergo multiple rounds of synchronous mitosis without cell division to form a giant cell [3–5, 7]. These multinucleate cells can contain more than 100 polyploid nuclei. The cells surrounding the giant cell undergo hypertrophy and hyperplasia to form a root gall [3–5, 7, 8]. Thus, expression of numerous host genes is modified to produce these extensive changes in the root. The J2 males and females molt three more times to reach maturity. The mature female produces an egg mass in a gelatinous protective sac that is extruded from the female nematode onto the root surface.
Within the nematode's esophageal gland cells, different proteins are produced to help the nematode establish a feeding site. Some of the proteins secreted by the nematode are injected into host cells and cause modification of the plant cells to form giant cells. Other proteins secreted by the nematode may interact with the host's extracellular receptors to influence signal transduction .
Similarly, gene expression is altered in the cells that are selected to be the feeding sites of the soybean cyst nematode. Klink et al. [9–13] demonstrated that numerous changes in gene expression occur in roots and in syncytial cells in soybean roots infected by either compatible or incompatible populations of soybean cyst nematodes. They used microarrays to study gene expression in laser capture microdissected (LCM) syncytium cells for susceptible and resistant reactions of soybean during infection with soybean cyst nematode (Heterodera glycines; SCN) [9, 10, 12]. Many genes were shown to be up- and down-regulated in both susceptible and resistant responses. Also, they identified many genes that are involved in plant-pathogen interactions, which provided new insights into the interaction between the cyst nematode and its host plant. In another microarray study by Klink et al., distinct expression patterns at different developmental stages of the SCN feeding site were detected in gene expression studies of syncytial cells collected by LCM from SCN-susceptible and resistant soybean cultivars. Gene expression patterns at the first stage were found to be similar in both the susceptible and resistant reactions, when the nematode first attempts to establish itself in the host. This stage is called the parasitism phase. The second stage depends on the defense response of the host plant. If the soybean plant exhibits resistance to the parasite, the nematode will fail to establish and will develop very slowly or die. If the plant is not resistant to the nematode, the soybean host and SCN are compatible and the nematode will establish its permanent feeding site. Using microarray analysis Ithal et al.[14, 15] studied transcript expression in syncytium cells induced by SCN in soybean roots after infection. They reported that several pathways are involved in the induction of syncytia. For example, genes involved in solidifying and lignifying the cell wall of the syncytium were shown to be up-regulated. Interestingly, they also reported down-regulation of the plant defense system, specifically the pathway leading to jasmonic acid. On the other hand, Klink et al. examined the response of a resistant cultivar of soybean against SCN by studying gene expression using microarrays. The levels of transcripts of genes encoding enzymes involved in jasmonic acid biosynthesis, phenylpropanoid biosynthesis, suberin biosynthesis, adenosyl-methionine biosynthesis, ethylene biosynthesis from methionine, flavonoid biosynthesis and the methionine salvage pathway were greatly altered during the defense response of soybean to H. glycines. Also, changes in gene expression have been monitored in Heterodera glycines- susceptible soybean cultivar (Kent) using microarray at 6, 12, and 24 hours after infection as well as 2, 4, 6, and 8 days after infection . In that study, the level of genes encoding WRKY6 transcription factor and lipoxygenase were shown to be up-regulated at most time points tested (12 hours after infection (hai) - 8 days after infection (dai)) after infection with Heterodera glycines.
Analysis of microarray data can be complex, as datasets are very large and it is difficult to analyze and integrate changes in metabolic pathways. Tremblay et al. used the PAICE program to analyze microarray data of soybean leaves infected with soybean rust (Phakopsora pachyrhizi). The PAICE program overlays gene expression results from microarrays onto biochemical pathways found in the Kyoto Encyclopedia of Genes and Genomes (KEGG). PAICE makes key changes in gene expression in biochemical pathways stand out and makes comparison of pathway changes among treatments and across time points easier.
New targets for nematode control could be developed through the identification of genes that are involved in the establishment of the nematode in the host plant and which participate in the formation of the permanent feeding site for the nematode. Ibrahim et al. were able to control M. incognita development in soybean plants after silencing four M. incognita genes using the RNA interference mechanism. In this study, portions of the genes encoding mitochondrial stress protein and tyrosine phosphatase were shown to have the greatest effect among four tested genes on nematode development and on the number of galls formed on the RNAi-expressing roots. Also, Dalzell et al. were able to silence the gene encoding FMRF amide-like peptide (flp) with 21 bp siRNAs, specific to that gene in infective (J2) stage juveniles of potato cyst nematode, Globodera pallida, and Meloidogyne incognita. Charlton e t al.  reduced the number of Meloidogyne incognita by 50% after simultaneous suppression of two genes, dual oxidase and a subunit of a signal peptidase required for the processing of nematode secreted proteins, respectively.
In this paper we used the 37,500 probe set Affymetrix Soybean GeneChip to assay gene transcript abundance in galls formed in soybean by M. incognita at two stages, small galls at 12 dai and large galls at 10 wai. These time points were chosen to contrast active nematode feeding at 12 dai with plant gene expression in a mature infection at 10 wai. The latter time point is particularly interesting as gene expression in plant roots after prolonged infection has not been reported previously. We used PAICE  software to visualize the expression of genes related to major biochemical pathways and we identified a number of different pathway genes that were affected by nematode infection. Although we are using biochemical pathways as a format to visualize gene expression results, the changes in gene expression overlaid on these pathways do not imply similar changes in the levels of the encoded protein. This study provides insights into the interaction between M. incognita and soybean and into the formation and maintenance of giant cells. Our long-term objective is to identify possible gene targets for manipulation to develop broad resistance of plants to RKN by using gene silencing technology or to over express certain soybean genes.
Plant and nematode procurement
Glycine max cv Williams 82 and M. incognita population LESREC (Lower Eastern Shore Research and Education Center) were grown in a greenhouse at the United State Department of Agriculture Soybean Genomics and Improvement Laboratory, Beltsville, MD, USA. M. incognita eggs were harvested from roots of G. max cv Williams 82 2-4 months after inoculation using a method modified from those previously described in Meyer et al. and Nitao . Soybean seedlings were grown in Promix (Premier Horticulture INC., Quakertown, PA, USA) for one week in 20 × 20 × 10 cm flats, then moved to sand (The Stone Store, Hanover, MD). Three thousands eggs were used to inoculate roots of 7 day old soybean seedlings (cv. Williams 82). Soybean roots at 12 dai, 10 wai, and control uninfected plants were washed with sterile water, flash frozen in liquid nitrogen, ground to a fine powder and frozen at -80°C until use. The infected roots were collected at 12 days after infection.
Nematodes were stained in infected roots using a modified protocol of Byrd et al. and Mahalingam et al.. Briefly, roots were washed in gently flowing tap water to remove soil and debris, cut to 2 cm segments, and placed in a small beaker, then soaked in 20-30 ml of 10% commercial Clorox (chlorine bleach, 5.25% NaOC1) for 3 min. The roots were rinsed in tap water and then transferred into a 50 ml glass bottle containing 20 ml of distilled water and left to boil in a microwave with loosened caps. A 500 μl of acid fuschin (0.15 g/10 ml H2O) and 500 μl of glacial acetic acid were added to the root samples and heated to boiling in a microwave twice. The roots were left to cool to room temperature before removing the excess stain with running tap water using Miracloth on the top of the bottle. A 20 ml of clearing reagent (1/3 lactic acid+ 1/3 glycerol + 1/3 distilled water) were added to roots and roots were left to destain for two hours to overnight. The nematodes were stained red as observed in the roots under a dissecting microscope. General chemical reagents were obtained from Sigma Chemical Co (St. Louis, MO).
RNA extraction and microarray analyses
RNA was extracted from 100 mg each of the three different root samples using the Ultra Clean Plant RNA Isolation Kit (MOBIO, Carlsbad, CA). Gene expression analysis was performed using the GeneChip® Soybean Genome Array (Affymetrix®, Santa Clara, CA, USA) containing more than 37,500 probe sets as described in Klink et a l. [9, 10]. In this GeneChip technology, each high density spot (gene) is represented by 11-probe pairs (11 μm feature size), which allows multiple independent measurement for each transcript. GeneChip® Soybean Genome Array details are available at the Affymetrix® website . The microarrays were hybridized and scanned at the Laboratory of Molecular Technology, SAIC-Frederick, National Cancer Institute at Frederick, Fredrick, MD, USA.
Affymetrix© soybean Genechip data was analyzed as described in Klink et al.[9, 10, 15] with additional analysis using PAICE . Briefly, microarray gene expression data was imported into MATLAB (Natick, MA) Bioinformatics Toolbox. Normalization of the probe sets was performed using RMA (Robust Multiarray Analysis). The resultant calculated output was the log base 2 of the expression values, enabling scaling of the dataset. Volcano plots were produced, which graphically illustrate gene expression fold-change with respect to statistical significance. The plots were produced using fold-changes >= |2.0| and p-values < = 0.05 with respect to the control (untreated roots of cv. Williams 82). The t-test was used in calculating p-values. False Discovery Rate analysis was further utilized against significantly expressed genes . The False Discovery Rate tool 'Significance Analysis of Microarrays' (SAM v3.0;  was used with a false discovery rate of 10%.
RT-PCR for confirmation of differential gene expression in infected soybean roots at 12-dai and 10-wai
To confirm the microarray results conducted obtained for M. incognita infected soybean roots at 12-dai and 10-wai, quantitative real time PCR (qRT-PCR) was performed on specific genes that were shown to be differentially expressed during the infection. Fourteen genes were chosen according to the changes in their expression at 12-dai and 10-wai (Table 1). The genes were classified and placed in three different groups according to their function [8, 9]; Table 1). Soybean ubiquitin-3 (Accession No. D28123) was used to normalize the results. RNA samples also used for microarray analysis were used in qRT-PCR analysis. RNA from three different biological replicates of each time point (12 dai and 10 wai), and the control (non-infected roots) were used to synthesize first-strand cDNA using the SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen, Carlesbad, CA) following the manufacturer's instructions. Quantitative real time PCR was performed using the Stratagene Mx3000P RT-PCR system as described by the manufacturer (Stratagene, La Jolla, CA) with 10 ng/reaction of cDNA for all genes. Primer sequences specific to each gene are presented in Table 2. Other controls for qRT-PCR included reactions containing no template or no reverse transcriptase. These controls resulted in no amplification. qRT-PCR was performed in two biological replicates and each reaction was replicated three times. DNA accumulation was detected by SYBR Green and the Ct value (cycle at which there is the first clearly detectable increase in the fluorescence) was calculated using the software provided with the Stratagene Mx3000P RT-PCR system. Dissociation curves showed amplification for only one product for each primer set. Data analysis was performed according to the sigmoidal method described by Rutledge and Stewart  for absolute quantification of transcripts. Absolute quantification of fluorescence intensity per ng dsDNA was obtained using 100 fg lambda gDNA in quadruplicate to calculate the optical calibration factor. Absolute quantification of the transcript level of the RNAi targeted genes was calculated using specific equations according to Ibrahim et al. and Tremblay et al. .
Biochemical pathway analysis was conducted using PAICE (P athway A nalysis and I ntegrated C oloring of E xperiments) . This software program maps expression levels of genes encoding enzymes found in the KEGG biochemical pathways database. Gene expression levels are denoted using color codes displayed at the pathway nodes depicted by enzyme EC numbers. Besides the pathway mapping feature, PAICE colors EC accessions using gradients of green and red to represent induced and suppressed gene expression respectively. The colors can be tailored to the analysis through the software package.
PAICE has the unique ability to color in yellow the expression of genes having multiple family members that lack a consensus in gene expression, i.e. some members are over-expressed and others are under-expressed.
Histological examination of RNK infection
At 12 dai, galls can be identified as small swellings along the soybean root (Figure 1a). Within the gall the nematode has started feeding and can be visualized by staining with acid fuchsin [24, 25] to monitor nematode invasion and development inside the roots (Figure 1b). Mature galls are present on soybean roots at 10 wai (Figure 1c). Within the gall, mature female M. incognita can be identified easily by staining (Figure 1d).
Transcript profiling of galls formed by M. incognita infection
A comparison of gene expression at12 dai compared to control led to the identification of 1867 genes with greater than 1.5-fold change in expression (Figure 2). Of these, 1278 genes increased and 589 genes decreased in expression. Transcripts encoding leghemaglobin C1 (AI973819) increased the most at 386-fold. The most down-regulated gene was BF070134 with homology to a putative senescence protein 12 and to ERD7; its transcripts were 77-fold lower than in the control. There were 2108 genes with altered expression in galls at 10 wai. Of these, 1460 genes increased in expression and 648 genes decreased in expression. The transcript of the gene encoding pathogenesis related protein PR1a (CK605838) increased the most at 258-fold. As in the 12 dai experiment, the most down-regulated gene was BF070134 with transcripts 172-fold lower than the control. When gene expression at 10 wai was compared directly to 12 dai, 827 genes were up-regulated, while 535 genes were down-regulated. In this case, transcripts of the gene encoding the cysteine-rich plant defense protein, defensin (PDF2.3; BI321308), increased the most at 63-fold, while the transcripts of the gene encoding xylene serine peptidase 1, subtilase (AW309540) decreased the most at 126-fold.
Mitosis and cell division
Our data reflect changes in expression of numerous genes involved in nuclear regulation and cell division in the gall at 12 dai and 10 wai (Table 3). For example two genes were increased in transcript abundance that are regulators of the cell cycle. These genes encode two NDR (n uclear D bf2-r elated) family members of AGC kinase, and they are increased in expression 24.5-fold (BI968028) and 5-fold (CA802074)) at 12 dai. By 10 wai genes of several NDR family members (NHL1 and NHL3) are expressed less than at 12 dai, i.e. BI968028 at 5.5 fold, AW156706 at -2.6 fold, and CF806406 at -9.6 fold. Transcripts of numerous cyclin-dependent protein kinases are in greater abundance at 12 dai than in control tissues (Table 3). This correlates well with the increase in nuclear division that occurs in giant cell. In addition, the gene encoding RBR1 retinoblastoma-related protein, which modulates E2F-transcripton factors that inhibit cell proliferation, is also increased at 12 dai. Expression of a gene encoding the plant hormone, phytosulfokine 3 growth factor (BK000117) involved in positive regulation of plant cell proliferation, is increased by 44-fold at 10 wai.
Cell wall modifications
Numerous genes involved in cell wall remodeling were shown to be differentially expressed after infection with M. incognita (Table 4). Genes encoding four members of the expansin enzyme family (expansins A1, A5, A16, and 45) were up-regulated at both time points (Table 4). Also, genes encoding endo-1,3-beta-glucanase [EC 22.214.171.124] family members were differentially expressed (Table 4). Most of them were up-regulated, while one member (AW831345) was down-regulated 27-fold in the 12 dai and 44-fold in the 10 wai. A gene encoding the cell wall-modifying xyloglucan endotransglycosylase/hydrolase [EC 126.96.36.199] was down-regulated at both time points, while the gene encoding endoxyloglucan transferase A2 was up-regulated at both time points (Table 4). Expression of a gene encoding pectin esterase [EC 188.8.131.52] increased 24-fold and 47.5-fold at 12 dai and 12 wai, respectively. In addition, a gene encoding cellulose synthase [EC 2.41.12] was down-regulated by 9.3-fold and 4.5-fold at 12 dai and 10 wai, respectively (Figure 3).
Also, in phenylpropanoid biosynthesis, genes encoding a family of 21 extensin peroxidases [EC 184.108.40.206] that participate in lignin biosynthesis were differentially regulated (Figure 4). The extensin gene with the highest increase in expression (AW309606) increased by 95-fold while the extension gene with the largest decrease in expression (BI970840) decreased by -16-fold.
Carbon and energy metabolism
The expression of numerous genes encoding enzymes in glycolysis, the tricarboxylic acid cycle and in amino sugar synthesis was altered. For example, the gene encoding UDP-glucuronate 4-epimerase [EC 220.127.116.11] was greatly down-regulated with a 21-fold decrease in transcript abundance. Also, the gene encoding GDP mannose 4,6-dehydratase [EC 18.104.22.168] was down-regulated 20.5- and 5.3-fold at 12 dai and 10 wai (data not shown). In the glycolysis pathway, many genes were up-regulated during infection, including genes encoding glucose-6-phosphate isomerase [EC 22.214.171.124], transcripts of which were increased by 14-fold at 12 dai (Figure 5)
There are multiple changes in expression of genes encoding enzymes of the alpha-linolenic acid pathway leading to several important defense-related compounds, including jasmonic acid (Figure 6). At 12 dai, the change in expression of genes encoding enzymes leading to jasmonic acid seems conflicting. For example, the gene encoding palmitoyl-CoA hydrolase [EC 126.96.36.199], an enzyme that is needed for the biosynthesis of the alpha-linolenic acid, is suppressed. Linolenic acid can be a substrate for lipoxygenase [EC 188.8.131.52]. Genes encoding six lipoxygenase family members were differentially expressed. Mostly of them were up-regulated with highest induction of 22-fold for one member (AW705829). One gene family member (BI426746) was down-regulated by 3.8-fold. In addition, some gene family members encoding allene oxide synthase (hydroperoxide dehydratase [EC 184.108.40.206]), leading to jasmonic acid synthesis, were up-regulated, while others were down-regulated. The abundance of transcripts of the gene encoding the next enzyme in the pathway, allene oxide cyclase [EC 220.127.116.11] was also decreased. Yet, a gene encoding OPDA reductase [EC 18.104.22.168] was up-regulated. In contrast, most of the genes encoding enzymes involve in jasmonic acid biosynthesis were down-regulated at 10 wai.
Genes encoding transcription factors and other proteins
Changes in gene transcripts were accompanied by changes in expression of transcription factors, especially those in the WRKY family of transcription factors. Our microarray results indicated that genes encoding several family members of WRKY genes were down-regulated at 12 dai, including genes encoding WRKY6, 15, and 22 (-10.5, -14.3, and -8.5 FC, respectively). In contrast, at 10 wai, genes encoding WRKY 21 and 70 were up-regulated at 117 and 42 FC, respectively.
Several pathogenesis-related proteins are induced in plants during infection with any pathogen or by wounding, including nematode infection, and induction of many of these is affected by salicylic acid, jasmonic acid or ethylene . In our microarray data, genes encoding pathogen related proteins (PR) such as PR3 were down-regulated at 12 dai and genes encoding PR3 at 10 wai showed a mixed response; some were up-regulated while others were down-regulated. The three copies of the pathogen related protein PR1 gene were over-expressed by 78.23, 97.56, and 138.50 fold, respectively (Data not shown).
Confirmation of differential gene expression by quantitative PCR
Quantitative PCR was conducted to confirm gene expression patterns revealed by microarray analysis. We measured transcript abundance of 14 genes that showed increased or decreased transcript abundance by microarray analysis (Table 5; Figure 7). The trends in up- or down-regulation of gene transcripts were consistent between microarrays and quantitative PCR results except for expression of the gene encoding lipoxygenase family member LOX1 at 10 wai. However, we did observe differences in levels of expression between methods. Differences in fold-change in gene expression as measured by microarray and qRT-PCR have been reported in previous studies [15, 18, 28].
When M. incognita infects and feeds in a soybean root, numerous genes are altered in expression in the root. M. incognita not only triggers the defense response of the root, but also redesigns the morphology of the root to form a gall and converts a soybean cell into a giant cell for feeding. The timing of these changes coincides with changes in gene expression as seen in our microarray experiments.
Regulators of the cell cycle and cell division
The cell cycle is regulated by two types of cyclin-dependent kinases. CDKA is required for cells to enter the S and M phases. CDKB1 and CDKB2 are expressed during the G2 and M phases and are responsible for the G2-M transition . Our microarray results indicate that genes encoding some members of the cyclin-dependent kinases family were differentially expressed at 12 dai and 10 wai. Over-expression of the gene encoding CKB2 at 12 dai (5.2 and 4.6 FC) correlates with the increase in plant nuclear division that occurs at the infection site due to M. incognita infection and feeding. Cells selected by M. incognita for feeding become multinucleate giant cells. This increase in nuclear division continues until 14 dai [3–5].
Cell cycle activation in giant cells has also been observed by Engler et al.. In that study, the transition from S to G2 and G2 to M phase was reported after the over-expression of a GUS gene driven by the cycB2 or cycA2 promoters at one to nine days after infection with M. incognita. Expression of the CDKB2 gene at 12 dai was higher than at 10 wai, i.e., 5.2- versus 3.1-fold, respectively (Table 1). Ramsay et al.  found that cyc D3 is essential to stimulate the G1 phase of the cell cycle in root knot nematode infected giant cells. In this investigation, the two types of CycD3 (CycD3.2 and CycD3.3) were shown to be relatively more strongly expressed as compared to that of LeCycA1.1, LeCycB1.1 and LeCycD3.1 in giant cells induced by Meloidogyne spp. compared with other cyclin-dependent kinases. They observed PCR amplification of CyD3.2 and CycD3.3, while no amplification of cycA.1, CycB1.1 and CycD3.1 was observed. Our data showed a suppression of gene expression of the gene encoding cycD3 which is important for the regulation of the G1-S transition. In addition, at 10 wai we found an increase in gene expression of CKS1 (CDK-Subunit 1), a protein that prevents CDK from driving the cell cycle into S phase. This result suggests that at the earlier time point, the giant cells reach maturity and then the genes required for nuclear division are turned off.
Cell wall modification and remodeling
Due to multiple nuclear divisions of selected cells with no coincident cell division, the giant cells sometimes reach more than 400-times the size of a normal cell and may contain more than one hundred nuclei . This expansion of the giant cell is accompanied by extensive cell wall modification. Our microarray data indicate altered expression of many genes involved in cell wall extension and remodeling (Table 4). We found that the genes encoding a cell wall-modifying xyloglucan endotransglycosylase/hydrolase [EC 22.214.171.124] and endoxyloglucan transferase A2 are differentially expressed in soybean roots after infection with M. incognita (12 dai and 10 wai). These enzymes play a role in softening and breaking down the cell wall . Genes encoding many endo-1,4-glucanase family members were up-regulated at both time points (12 dai and 10 wai). Endo-1,4-glucanase is involved in cell wall remodeling and expansion. LCM was used to isolate giant cells formed in tomato by M. javanica to examine gene expression . Numerous transcripts of genes involved in cell wall remodeling were also identified in the cDNA library of giant cells 4 dai, including transcripts of genes encoding pectin methylesterase and pectinesterase. Goellner et al.  identified genes encoding endo-1,4-glucanases that were up-regulated in feeding cells formed by M. incognita and cyst nematode in tobacco plants. Also, Mitchum et al. found that the promoter of an endo-1,4-β-glucanase gene was strongly activated in feeding cells formed by Meloidogyne incognita as indicated by strong promoter-driven GUS expression.
The increase in expression of the gene encoding expansin A in our results is consistent with other investigations, wherein the expansin (LeEXPA5) genes in A. thaliana and tomato were shown to be up-regulated in developing giant cells after infection with Meloidogyne[37, 38]. Moreover, down-regulation of genes encoding cellulose synthase and over-expression of genes encoding pectin esterase that degrades pectin to pectate coincide with a breakdown of the cell wall during the early time points of infection with Meloidogyne. Our results are consistent with those of Jammes et al., who found genes encoding pectin esterases and pectate lyases were activated in Arabidopsis thaliana roots after infection with Meloidogyne incognita and the cell wall loosening process was activated during the development of the giant cell as well. In giant cells formed in tomato by M. javanica, there is an 8-fold and 7.3-fold increase in expression of the gene encoding pectinesterase U1 precursor . Giant cells formed by M. javanica in roots of Arabidopsis were collected by LCM and analyzed by Barcala et al. . Genes encoding cellulose synthase, expansin, pectate lyase, endoxyloglucan transferase also were all up-regulated in these cells coinciding with cytoskeleton rearrangements that occur during giant cell development.
Nutrients supply for M. incognita
The nematode uses a large amount of plant resources to develop and reproduce. This demand for energy and carbon is reflected in the numerous genes involved in glycolysis and gluconeogenesis that are up-regulated in the soybean root (Figure 5). For example, we found many genes encoding enzymes in the glycolysis pathway and amino sugar synthesis to be up-regulated. Mostly, the changes in gene expression occurred early in infection (12 dai). In addition to their roles in pathways that provide energy and carbon for the nematode, some of these genes have an essential role in the soybean-M. incognita interaction. For example, at 12 dai, the gene encoding UDP-glucuronate 4-epimerase (EC 126.96.36.199) is highly down-regulated (-21 fold). In Arabidopsis, a mutation in this gene resulted in hyper-sensitivity to the cyst nematode, Heterodera schachtii. In Azospirillum brasilense, this enzyme is important for lipopolysaccharide synthesis which is important in the bacterium-plant root interaction. A mutation in this gene resulted in the failure of the bacteria to respond to several stresses and antimicrobial compounds. It also affected the ability of the bacteria to form biofilms . This enzyme may be important in allowing the host to respond to M. incognita invasion [42, 43].
In the glycolysis and gluconeogenesis pathways, many genes were shown to be up-regulated, including the gene encoding glucose-6-phosphate isomerase [EC: 188.8.131.52]. The gene encoding this enzyme was also shown to be up-regulated in cucumber plants after treatment with Trichoderma asperrellum T34 . The enzyme is essential in salinity tolerance in the alga Dunaliella salina.
Not only do nematodes require large quantities of carbon and energy from its host, they also use starch during juvenile development. Starch is stored in syncytia formed by Heterodera schachtii in roots of Arabidopsis. The authors postulate that the starch is also used to compensate for fluctuating levels of sugar during the course of nematode development and feeding. The high metabolic rate of cells was suggested by the increased expression of ribosomal genes in giant cells induced by M. javanica in tomato roots .
Degradation of the cell walls could result in release of sugar which in turn will be channeled to glycolysis as reflected in the activation of the genes encoding enzymes in the glycolytic pathway. Also, since some enzymes in glycolysis participate in the biosynthesis of pentose, purines and pyrimidines, there could be an increase in production of nucleotides required for DNA replication.
Plant defense system
When a nematode invades a plant root, it must repress or control the plant defense response so it can successfully establish its permanent feeding site [4–6]. Our microarray data showed significant changes in expression of genes related to the defense response against pathogens. The pathway leading to jasmonic acid biosynthesis is one of the pathways associated with pathogen resistance and genes in this pathway were significantly affected by Meloidogyne incognita infestation at both time points (12 dai and 10 wai; Figure 6). At 12 dai, six of seven members of the lipoxygenase gene family were up-regulated. Lipoxygenase (LOX) is essential to oxylipin biosynthesis and has an important function in the plant defense response against wounding and pathogen attack . Reduction of LOX activity resulted in an increase in susceptibility of transgenic potato plants to insect attack . Over-expression of the gene encoding lipoxygenase could mean that more 9-HPOTrE would be produced. This is one of the major products of lipoxygenase (Figure 6). Interestingly, 9-HPOTrE is involved in the activation of the plant defense response directly or through its metabolites. In potato plants, 9-HPOTrE is produced in response to injury or infection. The role of 9-HPOTrE in the plant defense response suggests that there may be another pathway of LOX-mediated defense response [-50]. 9-HPOTrE could also be a substrate for allene oxide synthase (Figure 6) to produce OPDA, the precursor for jasmonic acid. At 10 wai, the abundance of the lipoxygenase transcript was much lower than the 12 dai time point. Three of seven gene family members encoding lipoxygenase were down-regulated. Also, all of the allene oxide synthase family members were greatly down-regulated in addition to some other genes encoding enzymes in the jasmonic acid biosynthetic pathway (Figure 6). This indicates that at 12 dai the plant defense system is still struggling to fight the infestation, but after prolonged infection (10 wai) most of the genes that are responsible for the production of one major defense hormone, jasmonic acid, were turned off. Genes in this pathway could be targets for testing whether resistance to nematode infestation can be increased in transformed plants by over-expression of these genes.
We found a number of genes encoding PR proteins that were differentially expressed in soybean roots 12 dai with M. incognita. The genes encoding PR-1, PR-2 and PR-5 protein families increased in expression. Genes encoding PR-2 and PR-5 are expressed in sid mutants of Arabidopsis that do not accumulate SA. However, genes encoding PR1 are known to be induced by salicylic acid (SA) [50, 51]. This suggests that salicylic acid or its derivatives may be synthesized at 12 dai and 10 wai by M. incognita infection. Interestingly, there are two different possible routes to salicylic acid production . The pathway that has the most scientific support involves isochorismate synthase  and its genes are not represented on the microarray. The other pathway leading to SA production involves phenylalanine. In this pathway, we found a large increase in the expression of the gene encoding tyrosine aminotransferase [TAT; EC:184.108.40.206]. If the abundance of this enzyme is increased, then this could lead to increased phenylalanine production. Genes encoding phenylalanine ammonia-lyase [EC:220.127.116.11]; and salicylate 1-monooxygenase [1.14.13.-] were over-expressed 6.9 and 2.9 FC, respectively. SA induces the expression of PR-1 . In giant cells of tomato formed by M. incognita 4dai, several genes involved in the phenylpropanoid pathway were detected, notably phenylalanine ammonia-lyase and cinnamyl alcohol dehydrogenase (35). Transgenic tobacco over-expressing PR-1 was more resistant to blue mold and black shank caused by Peronospora tabacina and Phytophthora parasitica f. sp. nicotianae, respectively . Although SA treatment of tomato plants that were inoculated with Meloidogyne incognita did not completely eliminate nematode infection, it enhanced the synthesis of PR-1, which resulted in a significant increase in resistance to the nematode . In contrast, Portillo et al.  reported a decrease in transcripts of the PR-1 precursor in giant cells collected from tomato infected with M. javanica by LCM as measured using qRT-PCR. Genes encoding PR-3 and PR-4 family proteins are reported to be up-regulated by jasmonic acid and ethylene . Also, PR-4 showed ribonuclease activity against fungal protein in wheat . Furthermore, a gene encoding a pathogenesis-related protein was reported as up-regulated in the interaction of M. incognita with Arabidopsis at 21 dai . They also reported the increased expression of genes encoding several proteinase inhibitor proteins and leucine-rich repeat family proteins.
There are major changes in host gene expression between 12 dai and 10 wai by M. incognita. In the pathway leading to jasmonic acid synthesis, several genes are down-regulated at 10 wai. We identified changes in important genes and pathways during parasitism. Some host genes encode proteins that participate in the establishment of the feeding site, i.e., giant cells, required by M. incognita and for gall formation. These results provide new insights into host-parasite interactions. In the future, some of these genes may be used to control the plant parasitic nematode infestation through plant genetic engineering to over-express defense genes or silence genes that promote giant cell and gall formation.
soybean cyst nematode
polymerase chain reaction
day after infection
laser capture microdissection
week after infection
Kyoto encyclopedia of genes and genomes
Koenning SR, Overstreet C, Noling JW, Donald PA, Becker JO, Fortnum BA: Survey of Crop Losses in Response to Phytoparasitic Nematodes in the United States for 1994. Journal of Nematology. 1999, 31 (4S): 587-618.
Sasser JN, Freckman DW: A world perspective on nematology: the role of the society. Vistas on Nematology. Edited by: Veek JS, Dickson DW. 1987, Hyattsville, MD: Society of Nematologists, 7-14.
Caillaud MC, Dubreuil G, Quentin M, Barbeoch LP, Lecomte P, Engler J, Abad P, Rosso MN, Favery B: Root-knot nematodes manipulate plant cell functions during a compatible interaction. Journal of Plant Physiology. 2008, 165: 104-113. 10.1016/j.jplph.2007.05.007.
Bird DMcK: Manipulation of host gene expression by root-knot nematodes. J Parasitol. 1996, 82 (6): 881-888. 10.2307/3284193.
Williamson VM, Gleason CA: Plant-nematode interactions. Current Opinion in Plant Biology. 2003, 6: 327-333. 10.1016/S1369-5266(03)00059-1.
Bennett D: Root-knot nematodes in soybeans. 2010, Delta Farm Press, [http://deltafarmpress.com/soybeans/root-knot-nematodes-soybeans]
Gheysen G, Fenoll C: Gene expression in nematode feeding sites. Ann Rev Phytopathol. 2002, 40: 191-210. 10.1146/annurev.phyto.40.121201.093719.
Davis EL, Hussey RS, Baum TJ: Getting to the roots of parasitism by nematodes. Trends in Parasitol. 2004, 20 (3): 134-141. 10.1016/j.pt.2004.01.005.
Klink VP, Overall CC, Alkharouf NW, MacDonald MH, Matthews BF: A time-course comparative microarray analysis of an incompatible and compatible response by Glycine max (soybean) to Heterodera glycines (soybean cyst nematode) infection. Planta. 2007, 226: 1423-1447. 10.1007/s00425-007-0581-4.
Klink VP, Overall CC, Alkharouf NW, MacDonald MH, Matthews BF: Laser capture microdissection (LCM) and comparative microarray expression analysis of syncytial cells isolated from incompatible and compatible soybean (Glycine max) roots infected by the soybean cyst nematode (Heterodera glycines). Planta. 2007, 226: 1389-1409. 10.1007/s00425-007-0578-z.
Klink VP, Matthews BF: The use of laser capture microdissection to study the infection of Glycine max (soybean) by Heterodera glycines (soybean cyst nematode). Plant Signaling &Behavior. 2008, 3 (2): 1-3.
Klink VP, Hosseini P, Matsye PD, Alkharouf NW, Matthews BF: Syncytium gene expression in Glycine max [PI 88788] roots undergoing a resistant reaction to the parasitic nematode Heterodera glycines. Plant Physiology and Biochemistry. 2010, 48: 176-193. 10.1016/j.plaphy.2009.12.003.
Klink VP, Hosseini P, MacDonald MH, Alkharouf NW, Matthews BF: Population-specific gene expression in the plant pathogenic nematode Heterodera glycines exists prior to infection and during the onset of a resistant or susceptible reaction in the roots of the Glycine max genotype Peking. BMC Genomics. 2009, 10: [http://www.biomedcentral.com/1471-2164/10/111]
Ithal N, Recknor J, Nettleton D, Hearne L, Maier T, Baum TJ, Mitchum MG: Parallel Genome-Wide Expression Profiling of Host and Pathogen During Soybean Cyst Nematode Infection of Soybean. Molec Plant Microbe Interact. 2007, 20 (3): 293-305. 10.1094/MPMI-20-3-0293.
Ithal N, Recknor J, Nettleton D, Hearne L, Maier T, Baum TJ, Mitchum MG: Developmental Transcript Profiling of Cyst Nematode Feeding Cells in Soybean Roots. Molec Plant Microbe Interact. 2007, 20 (5): 510-525. 10.1094/MPMI-20-5-0510.
Klink VP, Hosseini P, Matsye PD, Alkharouf NW, Matthews BF: Syncytium gene expression in Glycine max [PI 88788] roots undergoing a resistant reaction to the parasitic nematode Heterodera glycines. Plant Physiology and Biochemistry. 2010, 48: 176-193. 10.1016/j.plaphy.2009.12.003.
Alkharouf NW, Klink VP, Chouikha IB, Beard HS, MacDonald MH, Meyer S, Knap HT, Khan R, Matthews BF: Time course microarray analyses reveal global changes in gene expression of susceptible Glycine max (soybean) roots during infection by Heterodera glycines (soybean cyst nematode). Planta. 2006, 224: 838-852. 10.1007/s00425-006-0270-8.
Tremblay A, Hosseini P, Alkharouf NW, Li S, Scheffler BE, Matthews BF: Transcriptome analysis of a compatible response by Glycine max to Phakopsora pachyrhizi infection. Plant Science. 2010, 179 (3): 183-193. 10.1016/j.plantsci.2010.04.011.
Ibrahim HMM, Alkharouf NW, Meyer SLF, Aly MAM, Gamal El-Din AY, Hussein EHA, Matthews BF: Post-transcriptional gene silencing of root-knot nematode in transformed soybean roots. Experimental Parasitology. 2010, 127 (1): 90-99.
Dalzell JJ, McMaster S, Fleming CC, Maule AG: Short interfering RNA-mediated gene silencing in Globodera pallida and Meloidogyne incognita infective stage juveniles. Internat Journal of Parasitology. 2010, 40: 91-100. 10.1016/j.ijpara.2009.07.003.
Charlton WL, Hare HYM, Bakhetia M, Hibbard JK, Atkinson HJ, McPherson MJ: Additive effects of plant expressed double-stranded RNAs on root-knot nematode development. Internat J Parasitol. 2010, 40 (7): 855-864. 10.1016/j.ijpara.2010.01.003.
Meyer SLF, Massoud SI, Chitwood DJ, Roberts DP: Evaluation of Trichoderma virens and Burkholderia cepacia for antagonistic activity against root-knot nematode, Meloidogyne incognita. Nematology. 2000, 2: 871-879. 10.1163/156854100750112815.
Nitao JK, Meyer SLF, Oliver JE, Schmidt WF, Chitwood DJ: Isolation of flavipin, a fungus compound antagonistic to plant-parasitic nematodes. Nematology. 2002, 4: 55-63. 10.1163/156854102760082203.
Byrd DW, Kirkpatrick T, Barker KR: An improved technique for clearing and staining plant tissue for detection of nematodes. Journal of Nematology. 1983, 15: 142-143.
Mahalingan R, Skorupska HT: Cytological expression of early response to infection by Heterodera glycines, Ichinohe in resistant PI 437654 soybean. Genome. 1996, 39: 986-998. 10.1139/g96-123.
The Affymetrix® website. [http://www.affymetrix.com/support/technical/datasheets/soybean_datasheet.pdf]
Tremblay A, Li S, Scheffler BE, Matthews BF: Laser capture microdissection and expressed sequence tag analysis of uredinia formed by Phakopsora pachyrhizi, the causal agent of Asian soybean rust. Physiological and Molecular Plant Pathology. 2009, 73: 163-174.
Rutledge RG, Stewart D: A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR. BMC Biotechnol. 2008, 8 (47): [http://www.biomedcentral.com/content/pdf/1472-6750-8-47.pdf]
Van Loon LC, Rep M, Pieterse CMJ: Significance of Inducible Defense-related Proteins in Infected Plants. Ann Rev Phytopathol. 2006, 44: 135-162. 10.1146/annurev.phyto.44.070505.143425.
Joube's J, Chamley ML, Delmas F, Walter J, Hernould M, Mouras A, Raymond P, Chevalier C: A New C-Type Cyclin-Dependent Kinase from Tomato Expressed in Dividing Tissues Does Not Interact with Mitotic and G1 Cyclins1. Plant Physiology. 2001, 126: 1403-1415. 10.1104/pp.126.4.1403.
Engler JA, Vleesschauwer VD, Burssens S, Celenza JLJ, Inzé D, Montagu MV, Engler G, Gheysen G: Molecular Markers and Cell Cycle Inhibitors Show the Importance of Cell Cycle Progression in Nematode-Induced Galls and Syncytia. The Plant Cell. 1999, 11: 793-807.
Ramsay K, Wang Z, Jones MGK: Using laser capture microdissection to study gene expression in early stages of giant cells induced by root-knot nematodes. Molecular Plant Pathology. 2004, 5 (6): 587-592. 10.1111/j.1364-3703.2004.00255.x.
Nishitani K: A genome-based approach to study the mechanisms by which cell-wall type is defined and constructed by the collaborative actions of cell-wall-related enzymes. J Plant Res. 2002, 115: 303-307. 10.1007/s10265-002-0032-z.
Fosu-Nyarko J, Jones MGK, Wang Z: Functional characterization of transcripts expressed in early-stage Meloidogyne javanica-induced giant cells isolated by laser microdissection. Molecular Plant Pathology. 2009, 10: 237-248. 10.1111/j.1364-3703.2008.00526.x.
Goellner M, Wang X, Davis EL: Endo-1, 4-Glucanase Expression in Compatible Plant-Nematode Interactions. The Plant Cell. 2001, 13: 2241-2255.
Mitchum MG, Sukno S, Wang X, Shani Z, Tsabary G, Shoseyov O, Davis EL: The promoter of the Arabidopsis thaliana Cel1 endo-1,4-Glucanase gene is differentially expressed in plant feeding cells induced by root-knot and cyst nematodes. Molecular Plant Pathology. 2004, 5 (3): 175-181. 10.1111/j.1364-3703.2004.00216.x.
Jammes F, Lecomte P, Engler JA, Bitton F, Magniette MLM, Renou JP, Abad P, Favery B: Genome-wide expression profiling of the host response to root-knot nematode infection in Arabidopsis. Plant J. 2005, 44: 447-458. 10.1111/j.1365-313X.2005.02532.x.
Gal TZ, Aussenberg ER, Burdman S, Kapulnik Y, Koltai H: Expression of a plant expansin is involved in the establishment of root knot nematode parasitism in tomato. Planta. 2006, 224: 155-162. 10.1007/s00425-005-0204-x.
Barcala M, Garcoa A, Cabrer J, Casson S, Lindsey K, Favery B, Garcia-Casado G, Solano R, Fenoll C, Escobar C: Early transcriptomic events in microdissected Arabidopsis nematode-induced giant cells. Plant J. 2010, 61: 698-712. 10.1111/j.1365-313X.2009.04098.x.
Wubben MJE, Rodermel SR, Baum TJ: Mutation of a UDP-glucose-4-epimerase alters nematode susceptibility and ethylene responses in Arabidopsis roots. The Plant Journal. 2004, 40: 712-724. 10.1111/j.1365-313X.2004.02257.x.
Lerner A, Sowinski SC, Valverde A, Lerner H, Dror R, Okon Y, Burdman S: The Azospirillum brasilense Sp7 noeJ and noel genes are involved in extracellular polysaccharide biosynthesis. Microbiology. 2009, 155: 4058-4068. 10.1099/mic.0.031807-0.
Tabata K, Takaoka T, Esaka M: Gene expression of ascorbic acid-related enzymes in tobacco. Photochemistry. 2002, 61: 631-635. 10.1016/S0031-9422(02)00367-9.
Conklin PL, Norris SR, Wheeler GL, Williams EH, Smirnoff N, Last RL: Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis. Proc Natl Acad Sci. 1999, 96: 4198-4203. 10.1073/pnas.96.7.4198.
Segarra G, Casanova1 E, Bellido D, Odena MA, Oliveira E, Trillas I: Proteome, salicylic acid, and jasmonic acid changes in cucumber plants inoculated with Trichoderma asperellum strain T34. Proteomics. 2007, 7: 3943-3952. 10.1002/pmic.200700173.
Cui L, Chai Y, Li J, Liu H, Zhang L, Xue L: Identification of a glucose-6-phosphate isomerase involved in adaptation to salt stress of Dunaliella salina. Journal of Applied Phycology. 2009, 22 (5): 563-568.
Niki T, Mitsuhara I, Seo S, Ohtsubo N, Ohashi Y: Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) protein genes in wounded mature tobacco leaves. Plant Cell Physiol. 1998, 39: 500-507.
Nishitani K: A genome-based approach to study the mechanisms by which cell-wall type is defined and constructed by the collaborative actions of cell-wall-related enzymes. J Plant Res. 2002, 115: 303-307. 10.1007/s10265-002-0032-z.
Gobel C, Feussner I, Schmidt A, Scheel D, Serrano JS, Hamberg M, Rosah S: Oxylipin Profiling Reveals the Preferential Stimulation of the 9-Lipoxygenase Pathway in Elicitor-treated Potato Cells. J Biol Chem. 2001, 276 (9): 6267-6273. 10.1074/jbc.M008606200.
Reddy PS, Kumar TC, Reddy MN, Reddanna SP: Differential formation of octadecadienoic acid and octadecatrienoic acid products in control and injured/infected potato tubers. Biochimica et Biophysica Acta. 2000, 1483: 294-300.
Oshima M, Matsuoka M, Yamamoto N, Tanaka Y, Kano-Murakami Y, Ozeki Y, Kato A, Harada N, Ohashi Y: Nucleotide sequence of the PR-1 gene of Nicotiana tabacum. FEBS Letters. 1987, 225: 243-246. 10.1016/0014-5793(87)81166-3.
White RF: Acetylslaicylic acid (aspirin) induces resitance to tobacco mosaic virus in tobacco. Virology. 1979, 99: 410-412. 10.1016/0042-6822(79)90019-9. 1979
Chen Z, Zhen Z, Huang J, Lai Z, Fan B: Biosynthesis of salicylic acid in plants. Plant Signaling & Behavior. 2009, 4 (6): 493-496. 10.4161/psb.4.6.8392. 2009
Wildermuth MC, Dewdney J, Wu G, Ausubel FM: Isochorismate synthase is required to synthesize salicylic acid for plant defence. Nature. 2001, 414: 562-565. 10.1038/35107108. 2001
Nawrath C, Métraux JP: Salicylic acid induction-deficient mutants of Arabidopsis express PR-2 and PR-5 and accumulate high levels of camalexin after pathogen inoculation. Plant Cell. 1999, 11: 1393-1404.
Nandi B, Sukul NC, Banewee N, Sengupta S, Das P, Sinha BSP: Induction of pathogenesis-related protein by salicylic acid and resistance to root-knot nematode in tomato. Indian J Nematology. 2003, 33 (2): 111-116.
Portillo M, Lindsey K, Casson S, Garcia-Casado G, Solano R, Fenol C, Escoba C: Isolation of RNA from laser-capture-microdissected giant cells at early differentiation stages suitable for differential transcriptome analysis. Molecular Plant Pathology. 2009, 10: 523-535. 10.1111/j.1364-3703.2009.00552.x.
Fuller VC, Lilley CJ, Atkinson HJ, Urwin PE: Differential gene expression in Arabidopsis following infection by plant-parasitic nematodes Meloidogyne incognita and Heterodera schachtii. Molec Plant Pathol. 2007, 8: 595-609. 10.1111/j.1364-3703.2007.00416.x.
The authors thank Peggy MacDonald for her excellent technical assistance, Leslie Wanner for her careful critical review of the manuscript, and Elizabeth Matthews for revision of figures. The authors gratefully acknowledge support from grants from United Soybean Board project number Y9254, the US-Egypt Science and Technology project number BIO8-001-002 and from the BioGreen 21 Program (no. PJ007031), Rural Development Administration, Republic of Korea.
HMMI, designed the study, managed plants and nematodes, isolated samples, analyzed data, performed qRT-PCR, wrote manuscript, revised manuscript; PH, performed microarray analyses, revised manuscript; NWA, performed statistical analyses of microarray analyses, revised manuscript; EHAH, coordinated and helped to draft the manuscript, critical reviewed manuscript; AEYGED, coordinated and helped to draft the manuscript, critical reviewed manuscript; MAMA, designed the study, critical reviewed manuscript; BFM, designed the study, analyzed data, contributed to writing and editing of the manuscript.
Authors’ original submitted files for images
Below are the links to the authors’ original submitted files for images.
About this article
Cite this article
Ibrahim, H.M., Hosseini, P., Alkharouf, N.W. et al. Analysis of Gene expression in soybean (Glycine max) roots in response to the root knot nematode Meloidogyne incognita using microarrays and KEGG pathways. BMC Genomics 12, 220 (2011). https://doi.org/10.1186/1471-2164-12-220
- Giant Cell
- Jasmonic Acid
- Laser Capture Microdissected
- Soybean Root