Time-dependent changes in gene expression induced by secreted amyloid precursor protein-alpha in the rat hippocampus
© Ryan et al.; licensee BioMed Central Ltd. 2013
Received: 14 November 2012
Accepted: 24 May 2013
Published: 6 June 2013
Differential processing of the amyloid precursor protein liberates either amyloid-ß, a causative agent of Alzheimer’s disease, or secreted amyloid precursor protein-alpha (sAPPα), which promotes neuroprotection, neurotrophism, neurogenesis and synaptic plasticity. The underlying molecular mechanisms recruited by sAPPα that underpin these considerable cellular effects are not well elucidated. As these effects are enduring, we hypothesised that regulation of gene expression may be of importance and examined temporally specific gene networks and pathways induced by sAPPα in rat hippocampal organotypic slice cultures. Slices were exposed to 1 nM sAPPα or phosphate buffered saline for 15 min, 2 h or 24 h and sAPPα-associated gene expression profiles were produced for each time-point using Affymetrix Rat Gene 1.0 ST arrays (moderated t-test using Limma: p < 0.05, and fold change ± 1.15).
Treatment of organotypic hippocampal slice cultures with 1 nM sAPPα induced temporally distinct gene expression profiles, including mRNA and microRNA associated with Alzheimer’s disease. Having demonstrated that treatment with human recombinant sAPPα was protective against N-methyl d-aspartate-induced toxicity, we next explored the sAPPα-induced gene expression profiles. Ingenuity Pathway Analysis predicted that short-term exposure to sAPPα elicited a multi-level transcriptional response, including upregulation of immediate early gene transcription factors (AP-1, Egr1), modulation of the chromatin environment, and apparent activation of the constitutive transcription factors CREB and NF-κB. Importantly, dynamic regulation of NF-κB appears to be integral to the transcriptional response across all time-points. In contrast, medium and long exposure to sAPPα resulted in an overall downregulation of gene expression. While these results suggest commonality between sAPPα and our previously reported analysis of plasticity-related gene expression, we found little crossover between these datasets. The gene networks formed following medium and long exposure to sAPPα were associated with inflammatory response, apoptosis, neurogenesis and cell survival; functions likely to be the basis of the neuroprotective effects of sAPPα.
Our results demonstrate that sAPPα rapidly and persistently regulates gene expression in rat hippocampus. This regulation is multi-level, temporally specific and is likely to underpin the neuroprotective effects of sAPPα.
Amyloid precursor protein (APP) is the parent molecule of the neurotoxic amyloid-β, implicated in the aetiology of Alzheimer’s disease. Amyloid-β is generated by sequential cleavage of APP by β and γ-secretases. However, the likely predominant route of APP processing is via α-secretase [1, 2], which not only precludes amyloid-β production but also generates secreted amyloid precursor protein alpha (sAPPα) [3, 4]. This molecule interacts with the β-secretase, BACE1, and directly inhibits amyloid-β production . Furthermore, mounting evidence implicates sAPPα in a wide variety of neuronal processes. It has been shown to both protect against glutamate toxicity in rat hippocampal neurons in vitro and in rat models of traumatic brain injury in vivo[6, 7] and promote neurite outgrowth in vitro[8, 9]. Furthermore, sAPPα has recently been shown to not only direct human embryonic stem cells into neuronal precursor cells [10, 11], but to increase proliferation of neural precursor cells from the rat hippocampus in vitro, and promote their proliferation in the mouse hippocampus in vivo. Additionally, sAPPα has been shown to enhance synaptic plasticity and restore memory deficits in rats and mice in vivo[13–16]. While a definitive cognate receptor for sAPPα has not yet been identified, sAPPα is known to activate intracellular signalling cascades in both neurons and glia and enhance synaptic protein synthesis in vitro[17–21]. As the reported physiological effects of sAPPα, are dependent on rapid and persistent alterations in gene expression, it is also likely that regulation of transcription is integral to the function of sAPPα.
Recently, expression of the transcription factor Egr1 was shown to mediate sAPPα stimulated axonal outgrowth of primary neurons from mice in vitro, and sAPPα has previously been shown to induce the expression of several neuroprotective genes in mouse organotypic hippocampal slices . This observation is particularly important as the hippocampus is a region vulnerable to the early neurodegenerative changes observed in Alzheimer’s disease. Interestingly, however, sAPPα knock-in mice show significantly lower expression of the plasticity related genes Arc, Egr2 and Fos. Furthermore, in general, the gene expression profiles of APP knockout and sAPPα knock-in mice do not differ substantially , questioning the role of the N-terminal region of APP in regulation of gene expression. In light of these somewhat conflicting data we sought to determine whether the application of sAPPα to organotypic hippocampal slice cultures for up to 24 h alters gene expression, and if so, whether the gene networks or biological pathways identified reflected the known physiological roles of sAPPα. We found that sAPPα rapidly enhances the transcription environment and alters subsequent gene expression in a manner that is likely to underpin the reported neuroprotective effects of sAPPα.
sAPPα protects against N-methyl d-aspartate toxicity
We have previously shown that purified human recombinant sAPPα produced in our laboratory  regulates protein synthesis , synaptic plasticity and memory , as well as neurogenesis . However, prior to determining the effects of sAPPα on gene expression, we sought to extend our assessment of the functionality of recombinant sAPPα. Accordingly, we tested whether sAPPα could protect against N-methyl-d-aspartate (NMDA)-induced excitotoxicity in hippocampal organotypic slice cultures.
Regulation of gene expression by sAPPα
To explore further how the effects of sAPPα on gene expression changed with time, the expression profiles were examined using a Venn diagram. This analysis showed that the three datasets were largely distinct (Figure 2B; See Additional file 3: Table S5). Only two genes were in common across all three datasets and only one was annotated. Interestingly, this gene, acyl-protein thioesterase 2, (Apt2) showed a distinct temporal response to sAPPα treatment, decreasing in expression at 15 min (−1.25 fold), increasing at 2 h (+1.4 fold) and decreased by 24 h (−1.8 fold). Activation of APT2 results in depalmitoylation of substrate proteins, a process important in regulating subcellular localisation of plasticity related ion channels and GAP43 [31, 32]. Thus, these data predict that regulation of Apt2 contributes to the plasticity-related effects of sAPPα.
Intersection of sAPPα and plasticity-regulated datasets
15 min sAPPα
20 min LTP
Dual specificity phosphatase 1
Early growth response 1
Jun B proto-oncogene
retinol binding protein 3, interstitial
FBJ osteosarcoma oncogene
2 h sAPPα
5 h LTP
GRP1 (general receptor for phosphoinositides 1)-associated scaffold protein
24 h sAPPα
24 h LTP
Aldehyde oxidase 1
Betaine-homocysteine methyltransferase 2
ELOVL family member 6, elongation of long chain fatty acids
Temporally specific regulation of microRNA by sAPPα
Predicted microRNA-regulated genes following miRvestigator Framework Analysis
Predicted miR-154 regulated genes
Transcript cluster ID
CDC28 protein kinase regulatory subunit 2
Crystallin, gamma D
Dual adaptor of phosphotyrosine and 3-phosphoinositides
Heat shock 70kDa protein 8
Leucine zipper protein 1
Olfactory receptor 1014
Olfactory receptor 1106
Olfactory receptor 1560
Olfactory receptor 1679
Olfactory receptor 75
Olfactory receptor 862
Polymerase (RNA) III (DNA directed) polypeptide G (32kD)
Similar to CG5435-PA
Similar to glyceraldehyde-3-phosphate dehydrogenase
Solute carrier family 5 (choline transporter), member 7
Suppression of tumorigenicity 18
Predicted miR-201* regulated genes
Transcript cluster ID
P . value
Olfactory receptor 139
Olfactory receptor 1532
Ribosomal protein L36a-like
Ribosomal protein, large, P2
Similar to RIKEN cDNA C430008C19
WW and C2 domain containing 2
sAPPα regulates genes linked to amyloid-ß toxicity and Alzheimer’s disease
Adapter protein TKS5
Mediates the neurotoxic effect of amyloid-β
Formyl peptide receptor 2
Activated by amyloid-β, may mediate inflammation seen in Alzheimer's disease
May interact with neurodegenerative disease-associated proteins
Peptidylprolyl isomerase F
An absence of this gene is neuroprotective against amyloid-β induced cell death
Tumour necrosis factor
Protects neurons against amyloid-β induced toxicity
Binds amyloid-β and prevents fibril formation in vitro
Chemokine (C-C motif) ligand 3
Protein levels are increased in Alzheimer's disease
FBJ murine osteosarcoma viral oncogene homolog
Gene expression is increased in Alzheimer's disease
Protein levels are increased in Alzheimer's disease
Expression is reduced in Alzheimer's disease
Regulator of BACE1 and decreased in Alzheimer's disease
Gene expression is increased in Alzheimer's disease
Regulator of G-protein signaling like 2
Gene associated with late onset Alzheimer's disease
sAPPα rapidly regulates transcriptional processes
This network also predicts that sAPPα mediates transcription via activation of the plasticity-related transcription factors, cAMP response element binding protein (CREB) and nuclear factor kappa B (NF-κB), that form central hubs within the network. While their expression levels remain unchanged, this is highly consistent with their roles as constitutive transcription factors regulated by post-transcriptional modification. Indeed, this network includes Dusp1 (+1.2 fold), a plasticity-related phosphatase and Map3k14 (+1.2 fold), a kinase known to phosphorylate and activate NF-κB, as well as the NF-κB regulatory molecule, Nf-κbid (+1.2 fold) [51, 52]. Together these data suggest that sAPPα treatment not only enhances the levels of transcription factors but also contributes to a dynamic interplay between a subset of protein kinases and phosphatases likely to maintain transcriptional activity.
Regulation of the chromatin environment is a third predicted mechanism by which sAPPα regulates expression of genes in this network. The network contains mediators of chromatin condensation (Kat2b: K(lysine) acetyltransferase; +1.2 fold) and histone biotinylation (Hlcs: holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase (ATP-hydrolysing)) ligase; +1.2 fold) [53, 54]. Upregulation of KAT2B is likely to result in reduced condensation of chromatin, occurring via acetylation of TNP2 (nuclear transition protein 2) a molecule also regulated within this network.
To further explore the 15 min dataset we used the functional annotation tool, DAVID. This highlighted Cognition and Sensory perception (Enrichment Score 2.2) as well as Response to corticosterone (Enrichment Scores 1.77, 1.34) as significantly enriched functions (Additional file 4: Table S6). Interestingly, there is an increase in basal glucocorticoid levels in Alzheimer’s disease, [55, 56]. Thus sAPPα may regulate the expression of genes associated with this pathway.
sAPPα-induced regulation of apoptosis and the inflammatory response
We next explored the gene networks affected by exposure to sAPPα for extended times. Following 2 h exposure to sAPPα, Inflammatory Response was the most significant biological function of the highest scoring network formed used IPA (Figure 4B; score 26). This network contains the proinflammatory cytokine Il1a, previously associated with Alzheimer’s disease, development and plasticity [57–61], immune associated genes (Cd80, Cr2, Cd27, Dapp1) and chemokines (Ccl3, Ccl4, Ccl21). These chemokines are involved in chemoattraction of immune cells to sites of tissue damage but have widespread non-immunological effects in the central nervous system, including regulation of neural cell proliferation, migration, survival and synaptic transmission [62, 63]. That sAPPα treatment promotes regulation of chemokine responses is also supported from analysis of the 2 h data set with the functional annotation tool, DAVID (Additional file 4: Table S6).
Like the 15 min network, NF-κB forms a central hub in this network, however, many of the genes contributing to the hub, including Ehf (ets homologous factor), which promotes apoptosis , are downregulated. Interestingly, using the upstream regulatory element analysis tool to analyse the genes upregulated within the 2 h dataset, it is predicted that sAPPα induces inhibition of the transcription factor, tumour protein 53 (p53), which is also an important regulator of apoptosis, and is increased in Alzheimer’s disease [65, 66] (Figure 5B). In addition, upstream regulatory element analysis using the entire 2 h dataset, predicts activation of the transcription factors AP-1, HMGB1 (high mobility group box protein) and STAT3 (signal transducer and activator of transcription 3) (Figure 5C) suggesting other mechanisms by which sAPPα coordinates this gene response.
sAPPα-induced long-term regulation of neurogenic, inflammatory response and cell survival pathways
As the reported biological functions of sAPPα, such as neuroprotection and regulation of memory, are enduring physiological changes, we explored the gene expression response induced by a 24 h exposure to sAPPα. To identify the functional relationships within the 24 h differentially expressed gene list, we used the DAVID functional annotation tool. This analysis identified Neurogenesis, Morphogenesis and Development as important biological functions affected 24 h post-sAPPα treatment (Enrichment Scores 2.26-1.48) (Additional file 4: Table S6, See Additional file 5: Figure S2).
We extended analysis of this dataset by interpretation of the highest scoring network derived using IPA, (Figure 4C). This network was composed of genes with overlapping biological functions, including upregulated genes related to cell proliferation (Cd80, Ubn1, Ptprr; validated by qPCR; Figure 3; Additional file 2: Table S4), regulation of genes likely to promote cell survival (Inkb1, Cd80, Sgk1, Fpr2, Cxcr4) and inhibit apoptosis (Gzmb; validated by qPCR; Figure 3; Cxcr4, Ifnb1, Sgk1). As sAPPα has previously been shown to promote growth, survival and proliferation in neurons and stem cells [10, 67, 68] these findings supports a role for sAPPα-induced gene expression in mediating these events.
In order to better understand the role that regulation of gene expression may play in mediating the wide-ranging cellular effects of sAPPα, we performed a global transcriptome analysis in organotypic hippocampal slice cultures treated with 1 nM sAPPα for 15 min, 2 h or 24 h. We focused on the hippocampus as this region is essential to learning and memory and is especially vulnerable to degeneration in Alzheimer’s disease . Our analysis demonstrated that sAPPα rapidly regulates gene expression by engaging multiple transcriptional regulatory mechanisms. This is evidenced by rapid and transient upregulation of inducible transcription factors (AP-1 complex, EGR1), temporally specific regulation of constitutively expressed transcription factors (NF-κB, CREB) and microRNA, as well as, modulation of the chromatin environment.
Network analysis showed that regulation of NF-κB is a consistent theme across all three time-points investigated. This supports previous work proposing that the neuroprotective effects of sAPPα are mediated through activation of NF-κB and subsequent enhanced activation of gene expression [70, 71]. sAPPα-induced NF-κB activation is implicated in protecting neural cells from apoptosis, therefore our results suggest a role for specifically regulated genes in this process . Interestingly, NF-κB has recently been intimately linked with amyloid-ß production [73, 74] and sAPPα modulates APP processing . Therefore, the control of amyloid-β production may be crucially dependent on the activation status of NF-κB, over which sAPPα has influence [70–72].
Increasing evidence points to the involvement of dysregulated microRNA in Alzheimer’s disease  and indeed an NF-κB-sensitive microRNA has been implicated in modulating the inflammatory circuit in Alzheimer’s disease . Our results highlight a role for microRNA in sAPPα-induced regulation of gene expression. We found that a short-term exposure to sAPPα was associated with upregulation of a subset of microRNA. As medium to long-term sAPPα exposure resulted in a general decrease in gene expression, we propose that this may be mediated by microRNA. Interestingly, we reported a similar general effect on gene expression following the induction of LTP, which we have shown to be associated with altered levels of mature microRNA . Our results also demonstrate that sAPPα induces a subset of plasticity-associated immediate early genes, however, we found little evidence that the sAPPα-induced gene response paralleled that of the LTP gene response. Therefore, post-translational modifications, and altered synaptic protein synthesis, are likely to mediate many of the effects of sAPPα on LTP [17, 77, 78].
It is of particular note that prolonged exposure to sAPPα resulted in persistent effects on gene expression that correlate closely with the documented neuroprotective and neurogenic roles of sAPPα. This interpretation is consistent with the only other microarray analysis of sAPPα-regulated gene expression in the hippocampus . This study reported that treatment of mouse organotypic slices with 1 nM sAPPα for 24 h also resulted in regulation of several neuroprotective genes. Interestingly, there was little coherence between the genes identified in this study and our own. This may result from differences in the array type used (Affymetrix MG-U74Av2 vs. Rat Gene 1.0 ST), the number of annotated genes investigated (~9, 000 vs ~28, 000), the statistical analyses performed (Wilcoxon signed rank test vs. moderate paired t-test) or species (mouse vs. rat).
While there is a paucity of data estimating the physiological levels of sAPPα, it has been estimated to fall within the pM range within plasma and brain homogenates [79–81], however, it is difficult to extrapolate these concentrations to the ex vivo model used in this study. Our studies highlight that 1 nM sAPPα is sufficient to modulate gene expression in ex vivo models and emphasizes that both medium and longer-term exposure to sAPPα elicits an inflammatory and immune gene response, which likely provides a neuroprotective environment. This neuroprotective setting appears to be strengthened by a parallel downregulation of apoptotic pathways and increases in cell proliferation and survival. Furthermore, we found evidence for the regulation of genes associated with neurogenesis. Recent evidence has also linked sAPPα with the induction of neurogenesis in the mammalian brain [10–12, 82]. As new brain cells have the capacity to integrate into previously established neural networks and contribute to hippocampal functioning , neurogenesis may also contribute to the sAPPα-induced neuroprotective and memory enhancing effects over the long-term.
In summary, our analyses consolidate the concept that sAPPα regulates gene expression. We provide evidence that this occurs in a temporally specific manner, and occurs through providing an environment conducive for transcription that results in activation of immediate early gene transcription factors, known to mediate neuroprotection and proliferation [84, 85], and regulation of microRNA. Furthermore, we demonstrate that gene networks constructed following medium and prolonged exposure to sAPPα reveal novel mechanisms likely to underpin and consolidate the neuroprotective stimulus induced by sAPPα in the hippocampus.
Organotypic hippocampal slice cultures
Preparation and maintenance
Organotypic hippocampal slice cultures were prepared from 7–10 day old Sprague Dawley rat pups of either sex according to the method of Stoppini et al., . The animals were deeply anaesthetized with ketamine (100 mg/kg, i.p.), using a protocol approved by the University of Otago Animal Ethics Committee to ensure minimal animal suffering. Brains were removed and placed in ice-cold filter-sterilized dissection media consisting of Minimum Essential Medium (MEM) containing Hank’s salts (95.5%, Gibco), penicillin-streptomycin solution (1%, Gibco), HEPES buffer solution (2.5%, Gibco) and 1M Tris–HCl (1%, Invitrogen, CA, USA). Hippocampi were then dissected free on an ice-cold glass plate and transverse slices cut at 400 μm on a Mcllwain tissue chopper (Mickle Laboratory Engineering, Surry, England). Separated slices were individually placed onto cell culture inserts (Millicell®, Millipore, MA, USA) held in 35 mm disposable petri dishes (Nunc, Denmark). To control for any gender differences, individual slices were randomised across all inserts. Slices were subsequently incubated in media containing MEM with Hank’s salts (50%), Hank’s balanced salt solution (25%, Gibco) and heat-inactivated horse serum (25%, Gibco) with 100 units of penicillin-streptomycin/ml and buffered to pH 7.2 with 1 M HEPES solution for three days at 37°C in a humidified incubator containing 5% CO2, and subsequently at 34°C. Culture medium was changed after the initial 24 h, and then every 3 days.
Slices were allocated to experimental groups after 10–11 days in vitro (DIV). Neuroprotection assays and gene expression experiments contained slices from 4 animals, with 4–5 slices on each insert. All treatments were conducted in culture medium in which the horse serum was replaced with the same volume of MEM. Recombinant human sAPPα was produced using human embryonic kidney-293 cells in which the sAPPα gene fragment was stably integrated .
Organotypic hippocampal slices were pre-treated with sAPPα (0.03-10 nM) or PBS for 24 h before treatment with NMDA (30 μM, 30 min) to induce partial, hippocampal-wide neuronal cell death. sAPPα was removed during the NMDA challenge, but subsequently reintroduced at the same concentration for a further 48 h.
Levels of cell death were determined 48 h after NMDA treatment using fluorescence imaging. Dead cells were labelled using the fluorescent dye propidium iodide (PI, ex. 536 nm, em. 617 nm). Emitted fluorescence was imaged with a Zeiss Axio Scope A1 microscope fitted with an LED illumination system and fluorescence filter cube (LED module ex. 540–580 nm; Zeiss filter set 43, ex. 545 nm- excitation, 605 nm-emission). Images were acquired with a Scion Corporation (Frederick, MD, USA) 12-bit colour camera (model CFW-1612C) and Scion Corporation VisiCapture image acquisition software. This software enabled us to reduce electronic and background image noise by collecting images that were the average of the previous five frames. For the purposes of analysis, each cultured slice was divided up into four principal areas CA1, CA3, inner blade of the dentate gurus (DG/ib), and outer blade of the dentate gyrus (DG/ob).
Cell death was quantified by measuring the average pixel intensity within a manually-selected region-of-interest (ROI) using ImageJ software (http://rsbweb.nih.gov/ij/download.html). For each image, the cell body layer was identified and manually selected as a ROI from which the average pixel intensity was calculated. The positioning of each ROI was dependent on the area to be analysed; CA1: identified as the cell body layer immediately above the DG; CA3: identified as the layer continuous with, and extending beyond, CA1 and ending before it entered the hilus of the DG. DG: divided into inner and outer blades with an approximate mid-point selected to demarcate the segments (Figure 1). The ROI size for each of the four areas was kept consistent between images.
For assessment of regional sAPPα effects, raw PI intensity values in the sAPPα groups were compared across the control and NMDA group values using a 1-way ANOVA with a post-hoc Bonferroni correction. Significance was set at p < 0.05.
Organotypic hippocampal slices were incubated with media containing 1 nM sAPPα or PBS for 15 min, 2 h or 24 h (n = 5 samples per treatment group, with each sample consisting of 5 randomly selected slices from 4 animals), rinsed with PBS, fixed in 70%(v/v) ETOH and snap frozen. Total RNA was prepared from organotypic hippocampal slices following tissue grinding on dry ice and shearing through a 22G × 1" Ultra Thin Wall Needle (Terumo®, Japan), using the NORGEN Total RNA Purification Kit (Norgen Biotek Corporation, Canada), plus DNAse I treatment step (Qiagen, Germany). RNA sample concentrations and integrity were determined using spectrophotometry (Nanodrop 1000; Thermo Scientific, USA) and a Bioanalyzer, using an RNA 6000 Nano Labchip (Bioanalyzer 2100; Agilent Techologies, USA). Only samples with an average RNA integrity number >8 were used [87, 88]. Array hybridizations were carried out at the Otago Genomics Facility (University of Otago), where RNA samples were biotin-labeled and hybridized to Affymetrix Rat Gene 1.0 ST arrays. These arrays cover 27342 annotated genes represented by ~26 probes spread across the full-length of each gene. A subset of microRNA are represented on the Rat GeneChip. The microRNA specific probes align to the stem loop sequences with the potential to identify both the primary and precursor forms of the transcripts.
Microarray data analysis
The Robust Multichip Average (RMA) package was used to normalize the data derived from treated and control samples (Sketch expression consul, Affymetrix, USA). In order to produce an inclusive list of sAPPα related genes and microRNA suitable for network analysis, differentially expressed genes were identified using two selection criteria: a threshold fold change cutoff (± 1.15) and a moderated t-test with a significance criterion of p < 0.05 . The t-statistic was generated using the Limma package, which utilizes a standard error moderated across all genes using a simple Bayesian model and produces p-values with greater degrees of freedom and hence greater reliability . More stringent selection criteria were not used in this study to avoid the risk of Type 2 error and unnecessarily limit the datasets for network analysis [90, 91].
To investigate potential microRNA likely to regulate the observed datasets, we used the web application miRvestigator Framework (http://mirvestigator.systemsbiology.net/) that identifies microRNA responsible for co-regulated gene expression patterns [33, 92].
Identification of biologically relevant networks and biological functions
Ingenuity pathway analysis, version 9 (IPA) (Ingenuity® Systems, http://www.ingenuity.com) was used to investigate interaction-based relationships between the genes and proteins encoded by the sAPPα-regulated gene expression sets. The gene sets, containing Affymetrix identifiers and corresponding expression values (p < 0.05), were submitted for analysis and gene networks were produced. Each network contains up to 35 genes and has an associated score derived from a p-value, indicating the expected likelihood of the genes being present in a network compared to that expected by chance. Scores of two or above have at least a 99% likelihood of not being generated by chance.
As the use of multiple tools is highly recommended for functional analysis of microarray data , we also applied an alternative analysis approach, Functional Annotation Clustering (DAVID ) to analyse the differentially expressed genes. In contrast to IPA, which is an interaction-based analysis, DAVID categorises the genes into groups based on Gene Ontology terms, and then displays similar annotations together. The total set of genes on the appropriate microarray was used as the background. The annotation terms are clustered based on the share of common genes and an EASE score, a modified Fisher exact p-value, is produced for each annotation term. Usually a p-value equal or smaller than 0.05 is considered strongly enriched in the annotation categories . Next, the annotation terms were clustered based on similar annotation terms and genes. The overall enrichment score of each cluster was based on the EASE scores of each annotation term. Clusters with a minimum enrichment score of 1.3 (equivalent to a p-value of 0.05) were deemed significant.
Real-time quantitative PCR
Selected biologically relevant differentially expressed genes were analysed by quantitative PCR (qPCR). Extracted RNA was reverse-transcribed to first strand cDNA using Superscript III (Invitrogen, CA, USA). Sixteen primer pairs were designed using Primer 3 (http://primer3.wi.mit.edu/) and obtained from Integrated DNA Technologies, (USA). Primer sequences are described in Additional file 6: Table S7. qPCR was performed using SYBR green mastermix (Roche, Switzerland) on a Roche Lightcycler 480. The qPCR validation included animals from the same RNA used in the microarray analysis. Results were normalised to the housekeeping genes Hypoxanthine phosphoribosyltransferase 1 (Hprt) and Peptidylprolyl isomerase A (Ppia) using the 2-∆∆CT method . These genes have consistently remained stable across numerous studies in the hippocampus eg [95, 96]. Significance was assessed using Student’s t-tests with the criterion set at p < 0.05 and 1.2 fold change.
Availability of supporting data
The data sets supporting the results of this article will be available in the ArrayExpress repository, accession number pending.
This study was supported by the New Zealand Health Research Council, the Royal Society of New Zealand Marsden Fund and the Neurological Foundation of New Zealand. We thank Dr. Shane Ohline and Ryan Abraham for technical assistance in producing the hippocampal organotypic slices and the neuroprotection assay, respectively. We also thank Les McNoe from the Otago Genomics Facility for assisting with the microarrays and Diane Guévremont for proofreading of the manuscript.
- Villemagne VL, Perez KA, Pike KE, Kok WM, Rowe CC, White AR, Bourgeat P, Salvado O, Bedo J, Hutton CA, et al: Blood-borne amyloid-beta dimer correlates with clinical markers of Alzheimer's disease. J Neurosci. 2010, 30 (18): 6315-6322. 10.1523/JNEUROSCI.5180-09.2010.View ArticlePubMedGoogle Scholar
- Moghekar A, Rao S, Li M, Ruben D, Mammen A, Tang X, O'Brien RJ: Large quantities of Abeta peptide are constitutively released during amyloid precursor protein metabolism in vivo and in vitro. J Biol Chem. 2011, 286 (18): 15989-15997. 10.1074/jbc.M110.191262.PubMed CentralView ArticlePubMedGoogle Scholar
- Turner PR, O'Connor K, Tate WP, Abraham WC: Roles of amyloid precursor protein and its fragments in regulating neural activity, plasticity and memory. Prog Neurobiol. 2003, 70 (1): 1-32. 10.1016/S0301-0082(03)00089-3.View ArticlePubMedGoogle Scholar
- Masters CL, Simms G, Weinman NA, Multhaup G, McDonald BL, Beyreuther K: Amyloid plaque core protein in Alzheimer disease and Down syndrome. Proc Natl Acad Sci U S A. 1985, 82 (12): 4245-4249. 10.1073/pnas.82.12.4245.PubMed CentralView ArticlePubMedGoogle Scholar
- Obregon D, Hou H, Deng J, Giunta B, Tian J, Darlington D, Shahaduzzaman M, Zhu Y, Mori T, Mattson MP, et al: Soluble amyloid precursor protein-alpha modulates beta-secretase activity and amyloid-beta generation. Nat Commun. 2012, 3: 777-PubMed CentralView ArticlePubMedGoogle Scholar
- Mattson MP, Cheng B, Culwell AR, Esch FS, Lieberburg I, Rydel RE: Evidence for excitoprotective and intraneuronal calcium-regulating roles for secreted forms of the beta-amyloid precursor protein. Neuron. 1993, 10 (2): 243-254. 10.1016/0896-6273(93)90315-I.View ArticlePubMedGoogle Scholar
- Thornton E, Vink R, Blumbergs PC, Van Den Heuvel C: Soluble amyloid precursor protein alpha reduces neuronal injury and improves functional outcome following diffuse traumatic brain injury in rats. Brain Res. 2006, 1094 (1): 38-46. 10.1016/j.brainres.2006.03.107.View ArticlePubMedGoogle Scholar
- Milward EA, Papadopoulos R, Fuller SJ, Moir RD, Small D, Beyreuther K, Masters CL: The amyloid protein precursor of Alzheimer's disease is a mediator of the effects of nerve growth factor on neurite outgrowth. Neuron. 1992, 9 (1): 129-137. 10.1016/0896-6273(92)90228-6.View ArticlePubMedGoogle Scholar
- Ninomiya H, Roch JM, Jin LW, Saitoh T: Secreted form of amyloid beta/A4 protein precursor (APP) binds to two distinct APP binding sites on rat B103 neuron-like cells through two different domains, but only one site is involved in neuritotropic activity. J Neurochem. 1994, 63 (2): 495-500.View ArticlePubMedGoogle Scholar
- Baratchi S, Evans J, Tate WP, Abraham WC, Connor B: Secreted amyloid precursor proteins promote proliferation and glial differentiation of adult hippocampal neural progenitor cells. Hippocampus. 2012, 22 (7): 1517-1527. 10.1002/hipo.20988.View ArticlePubMedGoogle Scholar
- Porayette P, Gallego MJ, Kaltcheva MM, Bowen RL, Vadakkadath Meethal S, Atwood CS: Differential processing of amyloid-beta precursor protein directs human embryonic stem cell proliferation and differentiation into neuronal precursor cells. J Biol Chem. 2009, 284 (35): 23806-23817. 10.1074/jbc.M109.026328.PubMed CentralView ArticlePubMedGoogle Scholar
- Caille I, Allinquant B, Dupont E, Bouillot C, Langer A, Muller U, Prochiantz A: Soluble form of amyloid precursor protein regulates proliferation of progenitors in the adult subventricular zone. Development. 2004, 131 (9): 2173-2181. 10.1242/dev.01103.View ArticlePubMedGoogle Scholar
- Bour A, Little S, Dodart JC, Kelche C, Mathis C: A secreted form of the beta-amyloid precursor protein (sAPP695) improves spatial recognition memory in OF1 mice. Neurobiol Learn Mem. 2004, 81 (1): 27-38. 10.1016/S1074-7427(03)00071-6.View ArticlePubMedGoogle Scholar
- Ishida A, Furukawa K, Keller JN, Mattson MP: Secreted form of beta-amyloid precursor protein shifts the frequency dependency for induction of LTD, and enhances LTP in hippocampal slices. NeuroReport. 1997, 8 (9–10): 2133-2137.View ArticlePubMedGoogle Scholar
- Meziane H, Dodart JC, Mathis C, Little S, Clemens J, Paul SM, Ungerer A: Memory-enhancing effects of secreted forms of the beta-amyloid precursor protein in normal and amnestic mice. Proc Natl Acad Sci USA. 1998, 95 (21): 12683-12688. 10.1073/pnas.95.21.12683.PubMed CentralView ArticlePubMedGoogle Scholar
- Taylor CJ, Ireland DR, Ballagh I, Bourne K, Marechal NM, Turner PR, Bilkey DK, Tate WP, Abraham WC: Endogenous secreted amyloid precursor protein-alpha regulates hippocampal NMDA receptor function, long-term potentiation and spatial memory. Neurobiol Dis. 2008, 31 (2): 250-260. 10.1016/j.nbd.2008.04.011.View ArticlePubMedGoogle Scholar
- Claasen AM, Guevremont D, Mason-Parker SE, Bourne K, Tate WP, Abraham WC, Williams JM: Secreted amyloid precursor protein-alpha upregulates synaptic protein synthesis by a protein kinase G-dependent mechanism. Neurosci Lett. 2009, 460 (1): 92-96. 10.1016/j.neulet.2009.05.040.View ArticlePubMedGoogle Scholar
- Barger SW, Mattson MP: The secreted form of the Alzheimer's β-amyloid precursor protein stimulates a membrane-associated guanylate cyclase. Biochem J. 1995, 311 (1): 45-47.PubMed CentralView ArticlePubMedGoogle Scholar
- Barger SW, Fiscus RR, Ruth P, Hofmann F, Mattson MP: Role of cyclic GMP in the regulation of neuronal calcium and survival by secreted forms of beta-amyloid precursor. J Neurochem. 1995, 64 (5): 2087-2096.View ArticlePubMedGoogle Scholar
- Cheng G, Yu Z, Zhou D, Mattson MP: Phosphatidylinositol-3-kinase-Akt kinase and p42/p44 mitogen-activated protein kinases mediate neurotrophic and excitoprotective actions of a secreted form of amyloid precursor protein. Exp Neurol. 2002, 175 (2): 407-414. 10.1006/exnr.2002.7920.View ArticlePubMedGoogle Scholar
- Gakhar-Koppole N, Hundeshagen P, Mandl C, Weyer SW, Allinquant B, Muller U, Ciccolini F: Activity requires soluble amyloid precursor protein alpha to promote neurite outgrowth in neural stem cell-derived neurons via activation of the MAPK pathway. Eur J Neurosci. 2008, 28 (5): 871-882. 10.1111/j.1460-9568.2008.06398.x.View ArticlePubMedGoogle Scholar
- Chasseigneaux S, Dinc L, Rose C, Chabret C, Coulpier F, Topilko P, Mauger G, Allinquant B: Secreted amyloid precursor protein beta and secreted amyloid precursor protein alpha induce axon outgrowth in vitro through Egr1 signaling pathway. PLoS One. 2011, 6 (1): e16301-10.1371/journal.pone.0016301.PubMed CentralView ArticlePubMedGoogle Scholar
- Stein TD, Anders NJ, DeCarli C, Chan SL, Mattson MP, Johnson JA: Neutralization of transthyretin reverses the neuroprotective effects of secreted amyloid precursor protein (APP) in APPSW mice resulting in tau phosphorylation and loss of hippocampal neurons: support for the amyloid hypothesis. J Neurosci. 2004, 24 (35): 7707-7717. 10.1523/JNEUROSCI.2211-04.2004.View ArticlePubMedGoogle Scholar
- Aydin D, Filippov MA, Tschape JA, Gretz N, Prinz M, Eils R, Brors B, Muller UC: Comparative transcriptome profiling of amyloid precursor protein family members in the adult cortex. BMC Genomics. 2011, 12: 160-10.1186/1471-2164-12-160.PubMed CentralView ArticlePubMedGoogle Scholar
- Turner PR, Bourne K, Garama D, Carne A, Abraham WC, Tate WP: Production, purification and functional validation of human secreted amyloid precursor proteins for use as neuropharmacological reagents. J Neurosci Methods. 2007, 164 (1): 68-74. 10.1016/j.jneumeth.2007.04.001.View ArticlePubMedGoogle Scholar
- Sinor JD, Du S, Venneti S, Blitzblau RC, Leszkiewicz DN, Rosenberg PA, Aizenman E: NMDA and glutamate evoke excitotoxicity at distinct cellular locations in rat cortical neurons in vitro. J Neurosci. 2000, 20 (23): 8831-8837.PubMedGoogle Scholar
- Soverchia L, Ubaldi M, Leonardi-Essmann F, Ciccocioppo R, Hardiman G: Microarrays–the challenge of preparing brain tissue samples. Addict Biol. 2005, 10 (1): 5-13. 10.1080/13556210412331327803.View ArticlePubMedGoogle Scholar
- Prinzen C, Trumbach D, Wurst W, Endres K, Postina R, Fahrenholz F: Differential gene expression in ADAM10 and mutant ADAM10 transgenic mice. BMC Genomics. 2009, 10: 66-10.1186/1471-2164-10-66.PubMed CentralView ArticlePubMedGoogle Scholar
- Ryan MM, Mason-Parker SE, Tate WP, Abraham WC, Williams JM: Rapidly induced gene networks following induction of long-term potentiation at perforant path synapses in vivo. Hippocampus. 2011, 21 (5): 541-553. 10.1002/hipo.20770.View ArticlePubMedGoogle Scholar
- Ryan MM, Ryan B, Kyrke-Smith M, Logan B, Tate WP, Abraham WC, Williams JM: Temporal profiling of gene networks associated with the late phase of long-term potentiation in vivo. PLoS One. 2012, 7 (7): e40538-10.1371/journal.pone.0040538.PubMed CentralView ArticlePubMedGoogle Scholar
- Tian L, McClafferty H, Knaus HG, Ruth P, Shipston MJ: Distinct acyl protein transferases and thioesterases control surface expression of calcium-activated potassium channels. J Biol Chem. 2012, 287 (18): 14718-14725. 10.1074/jbc.M111.335547.PubMed CentralView ArticlePubMedGoogle Scholar
- Fukata Y, Fukata M: Protein palmitoylation in neuronal development and synaptic plasticity. Nat Rev Neurosci. 2010, 11 (3): 161-175.View ArticlePubMedGoogle Scholar
- Bartel DP: MicroRNAs: target recognition and regulatory functions. Cell. 2009, 136 (2): 215-233. 10.1016/j.cell.2009.01.002.PubMed CentralView ArticlePubMedGoogle Scholar
- Di Bona D, Candore G, Franceschi C, Licastro F, Colonna-Romano G, Camma C, Lio D, Caruso C: Systematic review by meta-analyses on the possible role of TNF-alpha polymorphisms in association with Alzheimer's disease. Brain Res Rev. 2009, 61 (2): 60-68. 10.1016/j.brainresrev.2009.05.001.View ArticlePubMedGoogle Scholar
- Malinin NL, Wright S, Seubert P, Schenk D, Griswold-Prenner I: Amyloid-beta neurotoxicity is mediated by FISH adapter protein and ADAM12 metalloprotease activity. Proc Natl Acad Sci U S A. 2005, 102 (8): 3058-3063. 10.1073/pnas.0408237102.PubMed CentralView ArticlePubMedGoogle Scholar
- Le Y, Gong W, Tiffany HL, Tumanov A, Nedospasov S, Shen W, Dunlop NM, Gao JL, Murphy PM, Oppenheim JJ, et al: Amyloid (beta)42 activates a G-protein-coupled chemoattractant receptor, FPR-like-1. J Neurosci. 2001, 21 (2): RC123-PubMedGoogle Scholar
- Butterfield DA, Hardas SS, Lange ML: Oxidatively modified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Alzheimer's disease: many pathways to neurodegeneration. J Alzheimers Dis. 2010, 20 (2): 369-393.PubMed CentralPubMedGoogle Scholar
- Du H, Guo L, Fang F, Chen D, Sosunov AA, McKhann GM, Yan Y, Wang C, Zhang H, Molkentin JD, et al: Cyclophilin D deficiency attenuates mitochondrial and neuronal perturbation and ameliorates learning and memory in Alzheimer's disease. Nat Med. 2008, 14 (10): 1097-1105. 10.1038/nm.1868.PubMed CentralView ArticlePubMedGoogle Scholar
- Barger SW, Horster D, Furukawa K, Goodman Y, Krieglstein J, Mattson MP: Tumor necrosis factors alpha and beta protect neurons against amyloid beta-peptide toxicity: evidence for involvement of a kappa B-binding factor and attenuation of peroxide and Ca2+ accumulation. Proc Natl Acad Sci U S A. 1995, 92 (20): 9328-9332. 10.1073/pnas.92.20.9328.PubMed CentralView ArticlePubMedGoogle Scholar
- Schwarzman AL, Gregori L, Vitek MP, Lyubski S, Strittmatter WJ, Enghilde JJ, Bhasin R, Silverman J, Weisgraber KH, Coyle PK, et al: Transthyretin sequesters amyloid beta protein and prevents amyloid formation. Proc Natl Acad Sci U S A. 1994, 91 (18): 8368-8372. 10.1073/pnas.91.18.8368.PubMed CentralView ArticlePubMedGoogle Scholar
- Liao Y, Guan ZZ, Ravid R: [Changes of nuclear factor and inflammatory chemotactic factors in brain of patients with Alzheimer's disease]. Zhonghua Bing Li Xue Za Zhi. 2011, 40 (9): 585-589.PubMedGoogle Scholar
- Lu W, Mi R, Tang H, Liu S, Fan M, Wang L: Over-expression of c-fos mRNA in the hippocampal neurons in Alzheimer's disease. Chin Med J (Engl). 1998, 111 (1): 35-37.Google Scholar
- Blum-Degen D, Muller T, Kuhn W, Gerlach M, Przuntek H, Riederer P: Interleukin-1 beta and interleukin-6 are elevated in the cerebrospinal fluid of Alzheimer's and de novo Parkinson's disease patients. Neurosci Lett. 1995, 202 (1–2): 17-20.View ArticlePubMedGoogle Scholar
- Lukiw WJ: Micro-RNA speciation in fetal, adult and Alzheimer's disease hippocampus. NeuroReport. 2007, 18 (3): 297-300. 10.1097/WNR.0b013e3280148e8b.View ArticlePubMedGoogle Scholar
- Hebert SS, Horre K, Nicolai L, Papadopoulou AS, Mandemakers W, Silahtaroglu AN, Kauppinen S, Delacourte A, De Strooper B: Loss of microRNA cluster miR-29a/b-1 in sporadic Alzheimer's disease correlates with increased BACE1/beta-secretase expression. Proc Natl Acad Sci USA. 2008, 105 (17): 6415-6420. 10.1073/pnas.0710263105.PubMed CentralView ArticlePubMedGoogle Scholar
- Bensemain F, Hot D, Ferreira S, Dumont J, Bombois S, Maurage CA, Huot L, Hermant X, Levillain E, Hubans C, et al: Evidence for induction of the ornithine transcarbamylase expression in Alzheimer's disease. Mol Psychiatry. 2009, 14 (1): 106-116. 10.1038/sj.mp.4002089.View ArticlePubMedGoogle Scholar
- Liu F, Arias-Vasquez A, Sleegers K, Aulchenko YS, Kayser M, Sanchez-Juan P, Feng BJ, Bertoli-Avella AM, van Swieten J, Axenovich TI, et al: A genomewide screen for late-onset Alzheimer disease in a genetically isolated Dutch population. Am J Hum Genet. 2007, 81 (1): 17-31. 10.1086/518720.PubMed CentralView ArticlePubMedGoogle Scholar
- Pérez-Cadahía B, Drobic B, Davie JR: Activation and function of immediate-early genes in the nervous system. Biochem Cell Biol. 2011, 89 (1): 61-73. 10.1139/O10-138.View ArticlePubMedGoogle Scholar
- Chinenov Y, Kerppola TK: Close encounters of many kinds: Fos-Jun interactions that mediate transcription regulatory specificity. Oncogene. 2001, 20 (19): 2438-2452. 10.1038/sj.onc.1204385.View ArticlePubMedGoogle Scholar
- Tulchinsky E: Fos family members: regulation, structure and role in oncogenic transformation. Histol Histopathol. 2000, 15 (3): 921-928.PubMedGoogle Scholar
- Song HY, Regnier CH, Kirschning CJ, Goeddel DV, Rothe M: Tumor necrosis factor (TNF)-mediated kinase cascades: bifurcation of nuclear factor-kappaB and c-jun N-terminal kinase (JNK/SAPK) pathways at TNF receptor-associated factor 2. Proc Natl Acad Sci U S A. 1997, 94 (18): 9792-9796. 10.1073/pnas.94.18.9792.PubMed CentralView ArticlePubMedGoogle Scholar
- Huang EJ, Reichardt LF: Neurotrophins: roles in neuronal development and function. Annu Rev Neurosci. 2001, 24: 677-736. 10.1146/annurev.neuro.24.1.677.PubMed CentralView ArticlePubMedGoogle Scholar
- Camporeale G, Oommen AM, Griffin JB, Sarath G, Zempleni J: K12-biotinylated histone H4 marks heterochromatin in human lymphoblastoma cells. J Nutr Biochem. 2007, 18 (11): 760-768. 10.1016/j.jnutbio.2006.12.014.PubMed CentralView ArticlePubMedGoogle Scholar
- Bao B, Pestinger V, Hassan YI, Borgstahl GE, Kolar C, Zempleni J: Holocarboxylase synthetase is a chromatin protein and interacts directly with histone H3 to mediate biotinylation of K9 and K18. J Nutr Biochem. 2011, 22 (5): 470-475. 10.1016/j.jnutbio.2010.04.001.PubMed CentralView ArticlePubMedGoogle Scholar
- Roozendaal B, Phillips RG, Power AE, Brooke SM, Sapolsky RM, McGaugh JL: Memory retrieval impairment induced by hippocampal CA3 lesions is blocked by adrenocortical suppression. Nat Neurosci. 2001, 4 (12): 1169-1171. 10.1038/nn766.View ArticlePubMedGoogle Scholar
- Kulstad JJ, McMillan PJ, Leverenz JB, Cook DG, Green PS, Peskind ER, Wilkinson CW, Farris W, Mehta PD, Craft S: Effects of chronic glucocorticoid administration on insulin-degrading enzyme and amyloid-beta peptide in the aged macaque. J Neuropathol Exp Neurol. 2005, 64 (2): 139-146.View ArticlePubMedGoogle Scholar
- Griffin W, Stanley L, Ling C, White L, MacLeod V, Perrot L, White C, Araoz C: Brain interleukin 1 and S-100 immunoreactivity are elevated in Down syndrome and Alzheimer disease. Proc Natl Acad Sci U S A. 1989, 86 (19): 7611-7615. 10.1073/pnas.86.19.7611.PubMed CentralView ArticlePubMedGoogle Scholar
- Carter C: Convergence of genes implicated in Alzheimer's disease on the cerebral cholesterol shuttle: APP, cholesterol, lipoproteins, and atherosclerosis. Neurochem Int. 2007, 50 (1): 12-38. 10.1016/j.neuint.2006.07.007.View ArticlePubMedGoogle Scholar
- Dinarello C: Biologic basis for interleukin-1 in disease. Blood. 1996, 87 (6): 2095-2147.PubMedGoogle Scholar
- Politis A, Olgiati P, Malitas P, Albani D, Signorini A, Polito L, De Mauro S, Zisaki A, Piperi C, Stamouli E, et al: Vitamin B12 levels in Alzheimer's disease: association with clinical features and cytokine production. J Alzheimers Dis. 2010, 19 (2): 481-488.PubMedGoogle Scholar
- Spulber S, Bartfai T, Schultzberg M: IL-1/IL-1ra balance in the brain revisited - evidence from transgenic mouse models. Brain Behav Immun. 2009, 23 (5): 573-579. 10.1016/j.bbi.2009.02.015.View ArticlePubMedGoogle Scholar
- Bajetto A, Bonavia R, Barbero S, Schettini G: Characterization of chemokines and their receptors in the central nervous system: physiopathological implications. J Neurochem. 2002, 82 (6): 1311-1329. 10.1046/j.1471-4159.2002.01091.x.View ArticlePubMedGoogle Scholar
- Cartier L, Hartley O, Dubois-Dauphin M, Krause KH: Chemokine receptors in the central nervous system: role in brain inflammation and neurodegenerative diseases. Brain Res Brain Res Rev. 2005, 48 (1): 16-42. 10.1016/j.brainresrev.2004.07.021.View ArticlePubMedGoogle Scholar
- Fujikawa M, Katagiri T, Tugores A, Nakamura Y, Ishikawa F: ESE-3, an Ets family transcription factor, is up-regulated in cellular senescence. Cancer Sci. 2007, 98 (9): 1468-1475. 10.1111/j.1349-7006.2007.00543.x.View ArticlePubMedGoogle Scholar
- Dezor M, Dorszewska J, Florczak J, Kempisty B, Jaroszewska-Kolecka J, Rozycka A, Polrolniczak A, Bugaj R, Jagodzinski PP, Kozubski W: Expression of 8-oxoguanine DNA glycosylase 1 (OGG1) and the level of p53 and TNF-alphalpha proteins in peripheral lymphocytes of patients with Alzheimer's disease. Folia Neuropathol. 2011, 49 (2): 123-131.PubMedGoogle Scholar
- de la Monte SM, Wands JR: Molecular indices of oxidative stress and mitochondrial dysfunction occur early and often progress with severity of Alzheimer's disease. J Alzheimers Dis. 2006, 9 (2): 167-181.PubMedGoogle Scholar
- Smith-Swintosky VL, Pettigrew LC, Craddock SD, Culwell AR, Rydel RE, Mattson MP: Secreted forms of beta-amyloid precursor protein protect against ischemic brain injury. J Neurochem. 1994, 63 (2): 781-784.View ArticlePubMedGoogle Scholar
- Araki W, Kitaguchi N, Tokushima Y, Ishii K, Aratake H, Shimohama S, Nakamura S, Kimura J: Trophic effect of beta-amyloid precursor protein on cerebral cortical neurons in culture. Biochem Biophys Res Commun. 1991, 181 (1): 265-271. 10.1016/S0006-291X(05)81412-3.View ArticlePubMedGoogle Scholar
- Walsh DM, Selkoe DJ: Deciphering the Molecular Basis of Memory Failure in Alzheimer's Disease. Neuron. 2004, 44 (1): 181-193. 10.1016/j.neuron.2004.09.010.View ArticlePubMedGoogle Scholar
- Barger SW, Mattson MP: Participation of gene expression in the protection against amyloid beta-peptide toxicity by the beta-amyloid precursor protein. Ann N Y Acad Sci. 1996, 777: 303-309. 10.1111/j.1749-6632.1996.tb34437.x.View ArticlePubMedGoogle Scholar
- Barger SW, Mattson MP: Induction of neuroprotective kappa B-dependent transcription by secreted forms of the Alzheimer's beta-amyloid precursor. Brain Res Mol Brain Res. 1996, 40 (1): 116-126. 10.1016/0169-328X(96)00036-8.View ArticlePubMedGoogle Scholar
- Guo Q, Robinson N, Mattson MP: Secreted beta-amyloid precursor protein counteracts the proapoptotic action of mutant presenilin-1 by activation of NF-kappaB and stabilization of calcium homeostasis. J Biol Chem. 1998, 273 (20): 12341-12351. 10.1074/jbc.273.20.12341.View ArticlePubMedGoogle Scholar
- Bonizzi G, Karin M: The two NF-kappaB activation pathways and their role in innate and adaptive immunity. Trends Immunol. 2004, 25 (6): 280-288. 10.1016/j.it.2004.03.008.View ArticlePubMedGoogle Scholar
- Chami L, Buggia-Prevot V, Duplan E, Delprete D, Chami M, Peyron JF, Checler F: Nuclear Factor-kappaB Regulates betaAPP and beta- and gamma-Secretases Differently at Physiological and Supraphysiological Abeta Concentrations. J Biol Chem. 2012, 287 (29): 24573-24584. 10.1074/jbc.M111.333054.PubMed CentralView ArticlePubMedGoogle Scholar
- Cogswell JP, Ward J, Taylor IA, Waters M, Shi Y, Cannon B, Kelnar K, Kemppainen J, Brown D, Chen C, et al: Identification of miRNA changes in Alzheimer's disease brain and CSF yields putative biomarkers and insights into disease pathways. J Alzheimers Dis. 2008, 14 (1): 27-41.PubMedGoogle Scholar
- Lukiw WJ, Zhao Y, Cui JG: An NF-kappaB-sensitive micro RNA-146a-mediated inflammatory circuit in Alzheimer disease and in stressed human brain cells. J Biol Chem. 2008, 283 (46): 31315-31322. 10.1074/jbc.M805371200.PubMed CentralView ArticlePubMedGoogle Scholar
- Routtenberg A, Rekart JL: Post-translational protein modification as the substrate for long-lasting memory. Trends Neurosci. 2005, 28 (1): 12-19. 10.1016/j.tins.2004.11.006.View ArticlePubMedGoogle Scholar
- Sacktor TC: How does PKMzeta maintain long-term memory?. Nat Rev Neurosci. 2011, 12 (1): 9-15.View ArticlePubMedGoogle Scholar
- Maccecchini ML, Chang MY, Pan C, John V, Zetterberg H, Greig NH: Posiphen as a candidate drug to lower CSF amyloid precursor protein, amyloid-beta peptide and tau levels: target engagement, tolerability and pharmacokinetics in humans. J Neurol Neurosurg Psychiatry. 2012, 83 (9): 894-902. 10.1136/jnnp-2012-302589.PubMed CentralView ArticlePubMedGoogle Scholar
- Olsson A, Hoglund K, Sjogren M, Andreasen N, Minthon L, Lannfelt L, Buerger K, Moller HJ, Hampel H, Davidsson P, et al: Measurement of alpha- and beta-secretase cleaved amyloid precursor protein in cerebrospinal fluid from Alzheimer patients. Exp Neurol. 2003, 183 (1): 74-80. 10.1016/S0014-4886(03)00027-X.View ArticlePubMedGoogle Scholar
- Wu G, Sankaranarayanan S, Hsieh SH, Simon AJ, Savage MJ: Decrease in brain soluble amyloid precursor protein beta (sAPPbeta) in Alzheimer's disease cortex. J Neurosci Res. 2011, 89 (6): 822-832. 10.1002/jnr.22618.View ArticlePubMedGoogle Scholar
- Freude KK, Penjwini M, Davis JL, Laferla FM, Blurton-Jones M: Soluble amyloid precursor protein induces rapid neural differentiation of human embryonic stem cells. J Biol Chem. 2011, 286 (27): 24264-24274. 10.1074/jbc.M111.227421.PubMed CentralView ArticlePubMedGoogle Scholar
- Laplagne DA, Esposito MS, Piatti VC, Morgenstern NA, Zhao C, van Praag H, Gage FH, Schinder AF: Functional convergence of neurons generated in the developing and adult hippocampus. PLoS Biol. 2006, 4 (12): e409-10.1371/journal.pbio.0040409.PubMed CentralView ArticlePubMedGoogle Scholar
- Dhandapani KM, Hadman M, De Sevilla L, Wade MF, Mahesh VB, Brann DW: Astrocyte protection of neurons: role of transforming growth factor-beta signaling via a c-Jun-AP-1 protective pathway. J Biol Chem. 2003, 278 (44): 43329-43339. 10.1074/jbc.M305835200.View ArticlePubMedGoogle Scholar
- Kamphuis W, Dijk F, van Soest S, Bergen AA: Global gene expression profiling of ischemic preconditioning in the rat retina. Mol Vis. 2007, 13: 1020-1030.PubMed CentralPubMedGoogle Scholar
- Stoppini L, Buchs PA, Muller D: A simple method for organotypic cultures of nervous tissue. J Neurosci Methods. 1991, 37 (2): 173-182. 10.1016/0165-0270(91)90128-M.View ArticlePubMedGoogle Scholar
- Schroeder A, Mueller O, Stocker S, Salowsky R, Leiber M, Gassmann M, Lightfoot S, Menzel W, Granzow M, Ragg T: The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol. 2006, 7: 3-10.1186/1471-2199-7-3.PubMed CentralView ArticlePubMedGoogle Scholar
- Copois V, Bibeau F, Bascoul-Mollevi C, Salvetat N, Chalbos P, Bareil C, Candeil L, Fraslon C, Conseiller E, Granci V, et al: Impact of RNA degradation on gene expression profiles: assessment of different methods to reliably determine RNA quality. J Biotechnol. 2007, 127 (4): 549-559. 10.1016/j.jbiotec.2006.07.032.View ArticlePubMedGoogle Scholar
- Smyth GK: Linear models and empirical bayes methods for assessing differential expression in microarray experiments. Stat Appl Genet Mol Biol. 2004, 3: Article3-PubMedGoogle Scholar
- Dalman MR, Deeter A, Nimishakavi G, Duan ZH: Fold change and p-value cutoffs significantly alter microarray interpretations. BMC Bioinforma. 2012, 13 (Suppl 2): S11-10.1186/1471-2105-13-S2-S11.View ArticleGoogle Scholar
- van Iterson M, Boer JM, Menezes RX: Filtering. FDR and power. BMC Bioinforma. 2010, 11: 450-10.1186/1471-2105-11-450.View ArticleGoogle Scholar
- Plaisier CL, Bare JC, Baliga NS: miRvestigator: web application to identify miRNAs responsible for co-regulated gene expression patterns discovered through transcriptome profiling. Nucleic Acids Res. 2011, 39 (Web Server issue): W125-W131.PubMed CentralView ArticlePubMedGoogle Scholar
- da Huang W, Sherman BT, Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009, 4 (1): 44-57.View ArticlePubMedGoogle Scholar
- Winer J, Jung CK, Shackel I, Williams PM: Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal Biochem. 1999, 270 (1): 41-49. 10.1006/abio.1999.4085.View ArticlePubMedGoogle Scholar
- Pernot F, Dorandeu F, Beaup C, Peinnequin A: Selection of reference genes for real-time quantitative reverse transcription-polymerase chain reaction in hippocampal structure in a murine model of temporal lobe epilepsy with focal seizures. J Neurosci Res. 2010, 88 (5): 1000-1008.PubMedGoogle Scholar
- Santos AR, Duarte CB: Validation of internal control genes for expression studies: effects of the neurotrophin BDNF on hippocampal neurons. J Neurosci Res. 2008, 86 (16): 3684-3692. 10.1002/jnr.21796.View ArticlePubMedGoogle Scholar
- Hoffmann J, Pietrzik CU, Kummer MP, Twiesselmann C, Bauer C, Herzog V: Binding and selective detection of the secretory N-terminal domain of the alzheimer amyloid precursor protein on cell surfaces. J Histochem Cytochem. 1999, 47 (3): 373-382. 10.1177/002215549904700311.View ArticlePubMedGoogle Scholar
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