- Open Access
Homopharma: A new concept for exploring the molecular binding mechanisms and drug repurposing
© Chiu et al.; licensee BioMed Central Ltd. 2014
- Published: 8 December 2014
Drugs that simultaneously target multiple proteins often improve efficacy, particularly in the treatment of complex diseases such as cancers and central nervous system disorders. Many approaches have been proposed to identify the potential targets of a drug. Recently, we have introduced Space-Related Pharmamotif (SRPmotif) method to recognize the proteins that share similar binding environments. In addition, compounds with similar topology may bind to similar proteins and have similar protein-compound interactions. However, few studies have focused on exploring the relationships between binding environments and protein-compound interactions, which is important for understanding molecular binding mechanisms and helpful to be used in discovering drug repurposing.
In this study, we propose a new concept of "Homopharma", combining similar binding environments and protein-compound interaction profiles, to explore the molecular binding mechanisms and drug repurposing. A Homopharma consists of a set of proteins which have the conserved binding environment and a set of compounds that share similar structures and functional groups. These proteins and compounds present conserved interactions and similar physicochemical properties. Therefore, these compounds are often able to inhibit the proteins in a Homopharma. Our experimental results show that the proteins and compounds in a Homopharma often have similar protein-compound interactions, comprising conserved specific residues and functional sites. Based on the Homopharma concept, we selected four flavonoid derivatives and 32 human protein kinases for enzymatic profiling. Among these 128 bioassays, the IC50 of 56 and 25 flavonoid-kinase inhibitions are less than 10 μM and 1 μM, respectively. Furthermore, these experimental results suggest that these flavonoids can be used as anticancer compounds, such as oral and colorectal cancer drugs.
The experimental results show that the Homopharma is useful for identifying key binding environments of proteins and compounds and discovering new inhibitory effects. We believe that the Homopharma concept can have the potential for understanding molecular binding mechanisms and providing new clues for drug development.
- Protein Data Bank
- Thymidine Kinase
- Interact Residue
Developing a new drug is difficult and takes on average of 13 years as well as US$1.8 billion . Traditional approaches for rational drug design used the "one gene, one drug, one disease" paradigm to design exquisitely selective ligands of a single disease target. For example, gefitinib (trade name Iressa) and imatinib (trade name Gleevec) have been developed by this strategy and used for lung cancer and chronic myeloid leukemia, respectively. However, many drugs have been indicated that they can interact with more than one target protein [2–4]. Some unexpected efficacy can be linked to activity against additional targets, such as imatinib and PDGF receptor . Previous work has identified that a drug interacts with 6 targets on average by analyzing a drug-target network consisting of 802 drugs and 4,746 interactions . Many studies also suggest that drug development on targeting multiple proteins simultaneously can improve efficacy, particularly in the treatment of complex diseases (e.g. cancer and central nervous system disorders) [7, 8]. The strategy of pharmaceutical research, defined as the specific binding of a compound to two or more molecular targets, has variously been termed "network-based" discovery, "multi-targeted" drug design, "targeted polypharmacology", or "polypharmacology" [9–17].
One challenge of targeted polypharmacology is to identify the molecular targets that bind the compound. To recognize the proteins with similar binding sites of a given protein sequence or structure, the computational approaches of searching the protein sequence or structure databases are usually utilized [18–21]. As the number of protein structures is increasing, protein structures have been proposed to analyze the structural motifs and to describe the binding environments [22, 23]. However, most of these studies [24–28] search for similar local structures or binding sites (active sites) based on only one structural motif. Recently, we have introduced Space-Related Pharmamotif (SRPmotif) method  to identify pharma-interfaces (≥ 2 structural motifs) from a set of proteins which share similar binding environments. A pharma-interface is consists of a set of spatially discontinuous pharma-motifs which surround the ligand-binding site. In addition, compounds with similar topology would bind to the same or similar proteins and have similar protein-compound interactions [3, 4]. However, few studies focused on exploring the relationships between binding environments and protein-compound interactions. The atomic interactions between a compound and a protein are important when a compound target a protein. The combination of similar binding environments and protein-compound interactions provides the opportunities to explore the molecular binding mechanisms and is helpful for drug repurposing.
To address this issue, we proposed a new concept of "Homopharma" to describe similar binding environments and the relationships of interactions between proteins and compounds. A Homopharma is a set of proteins which have the conserved sub-binding environment at the protein-compound interfaces and a set of compounds with similar topology. Our results demonstrated that protein-compound complexes of a Homopharma perform similar protein-compound interactions and comprise conserved specific residues and important functional sites. According to the Homopharma concept, four similar flavonoid derivatives were tested against 32 human protein kinases using in vitro enzymatic profiling. The experimental results identified that the IC50 values of 56 and 25 flavonoid-kinase inhibitions are less than 10 μM and 1 μM, respectively. Some novel protein-compound interactions also suggest that these flavonoids could be used as anticancer compounds such as oral and colorectal cancer drugs. These results show that the concept Homopharma is not only useful for identifying potential targets of compounds, but can also reveal the key binding environment. Furthermore, it would be helpful for discovering the new usages for existing drugs. We believe that this approach can be further applied to understand molecular binding mechanisms and take a new direction on drug discovery.
Homopharma and pharma-interfaces
Step 1: Search candidates of a pharma-interface
SRPmotif is utilized to rapidly search potential targets having similar pharma-interfaces (Figure 1A). A pharma-interface can be described as follows: (1) a pharma-interface is a conserved binding interface of multiple proteins which share similar interfaces and are often inhibited by similar compounds; (2) a pharma-interface consists of a set of spatially discontinuous pharma-motifs; (3) a pharma-motif is a short conserved peptide forming a specific sub-interface with interacting residues and specific physico-chemical properties. Each discontinuous pharma-motif of a query complex is translated to 1D sequence with 23 structural alphabets using κ and α angles defined in the DSSP program  and applied to search against Protein Data Bank (PDB)  by 3D-BLAST [32, 33]. The candidate structures are then superimposed with the discontinuous pharma-motifs of the query complex by DALI , which is a structural alignment tool based on contact similarity patterns. The candidate protein-compound complexes of root-mean-square deviation (RMSD) ≤ 3Å are reserved. All search parameters of SRPmotif are default. Accordingly, a pharma-interface which consists of several pharma-motifs is constructed based on the conserved binding interfaces. The complexes of the pharma-interface are then used to estimate the protein-compound interaction profile and to evaluate interaction similarities.
Step 2: Calculate protein-compound interaction profiles
A compound would bind to proteins having similar binding environments; as well as proteins often interact with compounds of similar topology. These protein-compound interactions provide the opportunities to explore the molecular binding mechanisms. After identifications of candidate complexes, similarities between all crystal ligands of complexes are calculated by atom pairs (AP) . The structures whose crystal ligands with significant topology similarity (≥ 0.6) to any compound are reserved. To measure the similarity between protein-compound interactions, a multiple protein sequence (or structure) alignment is required. In this work, a multiple protein sequence alignment is utilized and generated by the reserved structures and T-coffee . Finally, the protein-compound interaction profile of candidate complexes is calculated based on iGEMDOCK . Different interaction types (electrostatic, hydrogen bonding, and van der Waals) and interactions of main- and side-chain heavy atoms are considered separately.
Step 3: Cluster interaction profiles using interaction similarity
where A i and W i is the interaction and the conservation weight in position i of the MSA, respectively.
Consequently, the protein-compound interactions are clustered by two-way hierarchical clustering. These interactions are divided into several Homopharma groups, which consist of similar binding environment, compound topology and interactions.
Protein-ligand complex validation dataset
To verify the Homopharma concept, protein-compound complexes were obtained from PDB. The complexes are eliminated when compounds are metal ions, surface compounds, cofactors, and small compounds (heavy atoms < 6). Two protein-compound pairs are grouped according to the same proteins or compounds. The protein-compound groups are filtered by number of complexes below 50% of all possible protein-compound combinations. Two groups which overlap with more than 50% protein or compounds are merged. Finally, 176 protein-compound groups that contain 1,325 complex structures including 672 proteins and 471 compounds are used as validation dataset.
Evaluations of compound similarity
A protein-compound complex having significant compound similarity is considered as a candidate of a Homopharma. In order to decide the threshold of compound topology similarity, we collected 3,151 crystal complex structures of 957 proteins. The distribution demonstrates the relationships between percentage and compound topology similarity (Figure S1A in Additional file 1). The precision, recall, and f-measure values were evaluated and the highest F-measure is at 0.36 when the compound topology similarity is 0.6 as well as the precision value is over 0.4. For example, methotrexate (MTX) is the first generation of dihydrofolate reductase (DHFR) inhibitor; the values of compound similarity of COP and DTM to MTX are 0.7 and 0.3, respectively (Figure S1B in Additional file 1). The compound COP is also the first generation of DHFR inhibitor and has similar topology to MTX; however, DTM is the second generation inhibitor and performs a different binding mode. Another compound estrogen (EST) presents similar results. The compound DES (similarity: 0.8) is the estrogen receptor agonist that present the same function with EST, where RAL (similarity: 0.2) is just an estrogen receptor antagonist.
Evaluations of interaction similarity
Example analysis: thymidine kinase
The complexes in a Homopharma present similar binding modes and have the conserved interacting residues (Figures 3B and 3C). Moreover, the conserved interacting residues of these Homopharma groups are often related to ligand binding and catalytic residues. For example, Figure 3B shows the superimposed structures of Homopharma 1 and conserved interacting residues (residue numbering of PDB code: 1OI3). Several interacting residues (H58, E83, W88, and Y172) are highly conserved in Homopharma 1. Residue E225 is important to form hydrogen bonding that stabilizes the LID region for TK catalytic reaction and H58L/M128F/Y172F triple mutant has also been indicated that related to the resistance of current anti-TK drugs, such as acyclovir . The conserved interaction profiles are presented in Figure S2A in Additional file 1. However, Homopharma 2 has different conserved interacting residues to Homopharma 1 (Figures 3C and S2B in Additional file 1). Residues H58, E83, W88, and Y172 are also conserved in Homopharma 1 as well; however, the specific conserved residue R222 of Homopharma 2 interacts with phosphate group of TK substrates . Furthermore, Homopharma 3 and 4 also present different binding environments (Figures S2C and S2D in Additional file 1).
Example analysis: protein kinases and flavonoid derivatives
Flavonoids are a class of plant secondary metabolites and have more than 5,000 kinds of derivatives. Flavonoid derivatives have been crystallized within more than 300 protein complex structures, such as protein kinases. Protein kinases play important roles in cell growth and signal transduction [39, 40]. Protein kinases are one of the most important classes of drug targets, because the deregulation of kinase functions is often implicated in many diseases, such as cancers and neurological and metabolic diseases [40–42]. Although protein kinases share a highly conserved ATP binding environment; however, identical kinase inhibitor has varied inhibitory effects on different kinases [3, 4]. Because protein kinases are involved in many complex diseases, many flavonoid derivatives whose biological activities are known on kinases may be repositioned to potential candidates of other diseases.
Homopharma 4 also performs the conserved interactions to hinge motif (Figure 4C). However, these compounds of Homopharma 4 belong to Type I kinase inhibitors, which target kinases with active conformation and directly compete with ATP binding. Furthermore, compounds of Homopharma 2 are also Type I kinase inhibitors, but have different topology and interacting residues (Figure S3B in Additional file 1). These compounds perform more interactions with residues of C-lobe pocket. Previous study showed that the conserved aspartic acid in C-loop is important in positioning the substrate hydroxyl for in-line nucleophilic attack . Similar observations can be found in other Homopharma groups (Figure S3 in Additional file 1).
Kinase profiling experiment results
List of 32 tested protein kinases for in vitro enzymatic profiling.
Kinase SK number
v-akt murine thymoma viral oncogene homolog 2
dystrophia myotonica-protein kinase
protein kinase C, alpha
ribosomal protein S6 kinase, 90kDa, polypeptide 1
calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma
calcium/calmodulin-dependent protein kinase IV
CHK1 checkpoint homolog (S. pombe)
CHK2 checkpoint homolog (S. pombe)
casein kinase 1, gamma 1
vaccinia related kinase 1
cyclin-dependent kinase 6
CDC-like kinase 3
casein kinase 2, alpha 1 polypeptide
mitogen-activated protein kinase 10
mitogen-activated protein kinase 14
mitogen-activated protein kinase 3
mitogen-activated protein kinase kinase 1
serine/threonine kinase 10
serine/threonine kinase 24 (STE20 homolog, yeast)
epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2, Pfeiffer syndrome)
insulin-like growth factor 1 receptor
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
lymphocyte-specific protein tyrosine kinase
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
spleen tyrosine kinase
activin A receptor, type I
LIM domain kinase 1
mitogen-activated protein kinase kinase kinase 7
v-raf-1 murine leukemia viral oncogene homolog 1
In vitro enzymatic profiling of the 32 member kinase panel was performed at Reaction Biology Corporation (http://www.reactionbiology.com, Malvern, PA) using the "HotSpot" assay platform . Each kinase activity assay was performed in duplicate with inhibitor concentration of 10 μM and an ATP concentration of 10 μM. The experimental results show that three similar flavonoids (quercetin, JMY51, and JMY65) have similar inhibitory effects on the tested 32 kinases. The results indicate that kinases in the same Homopharma group are inhibited by similar compounds. Our results also discover novel interactions, for example, JMY65 has SRC, LCK, and KIT inhibitory activities (Figures 5C and 5E). Moreover, JMY65 could be used to treat oral cancer because of its inhibitory effects on AKT2, IGF1R, EGFR, and MAPK3 (Figures 5C and 5E), which are highly expressed in oral cancer cells. Another compound JMY84 could also be used as drug candidates of colorectal cancer due to its effects on CHEK2, LIMK1, and FGFR1 (Figures 5D and 5E).
In this study, we propose a new concept of "Homopharma", combining similar binding environments and protein-compound interaction profiles, to explore the molecular binding mechanisms and drug repurposing. A Homopharma consists of a set of proteins sharing conserved binding environment and a set of compounds which have similar structures and functional groups. In a Homopharma, the compounds are often able to inhibit these proteins because their interactions and physicochemical properties are often consensus. Experimental results show that protein-compound complexes of a Homopharma often perform similar interactions in which formed by conserved binding residues (protein sites) and similar important functional groups (compound sites). According to the Homopharma concept, we successfully discovered 56 flavonoid-kinase inhibitions (IC50 ≤ 10 μM) by in vitro enzymatic profiling. Among these 56 inhibitions, the IC50 values of 25 bioassays are less than 1 μM and these flavonoids can be considered as potential anticancer compounds. These results show that Homopharma is not only useful to identify potential targets of compounds, but also can reveal the key binding environments. We believe that the Homopharma concept is useful for understanding molecular binding mechanisms and providing new opportunities for drug repurposing.
This paper was supported by Ministry of Science and Technology, partial supports of Ministry of Education and National Health Research Institutes (NHRI-EX100-10009PI). This paper is also particularly supported by "Aim for the Top University Plan" of the National Chiao Tung University and Ministry of Education. JMY also thanks Core Facility for Protein Structural Analysis supported by National Core Facility Program for Biotechnology.
The publication costs for this paper were funded by the Taiwan Ministry of Science and Technology grant MOST103-2113-M-009-010 awarded to JMY.
This article has been published as part of BMC Genomics Volume 15 Supplement 9, 2014: Thirteenth International Conference on Bioinformatics (InCoB2014): Computational Biology. The full contents of the supplement are available online at http://www.biomedcentral.com/bmcgenomics/supplements/15/S9.
- Paul SM, Mytelka DS, Dunwiddie CT, Persinger CC, Munos BH, Lindborg SR, Schacht AL: How to improve R&D productivity: the pharmaceutical industry's grand challenge. Nature Reviews Drug Discovery. 2010, 9 (3): 203-214.PubMedGoogle Scholar
- Karaman MW, Herrgard S, Treiber DK, Gallant P, Atteridge CE, Campbell BT, Chan KW, Ciceri P, Davis MI, Edeen PT, et al: A quantitative analysis of kinase inhibitor selectivity. Nature Biotechnology. 2008, 26 (1): 127-132. 10.1038/nbt1358.PubMedView ArticleGoogle Scholar
- Anastassiadis T, Deacon SW, Devarajan K, Ma HC, Peterson JR: Comprehensive assay of kinase catalytic activity reveals features of kinase inhibitor selectivity. Nature Biotechnology. 2011, 29 (11): 1039-1045. 10.1038/nbt.2017.PubMedPubMed CentralView ArticleGoogle Scholar
- Davis MI, Hunt JP, Herrgard S, Ciceri P, Wodicka LM, Pallares G, Hocker M, Treiber DK, Zarrinkar PP: Comprehensive analysis of kinase inhibitor selectivity. Nature Biotechnology. 2011, 29 (11): 1046-1051. 10.1038/nbt.1990.PubMedView ArticleGoogle Scholar
- Frantz S: Drug discovery: playing dirty. Nature. 437 (7061): 942-943.Google Scholar
- Mestres J, Gregori-Puigjane E, Valverde S, Sole RV: The topology of drug-target interaction networks: implicit dependence on drug properties and target families. Molecular Biosystems. 2009, 5 (9): 1051-1057. 10.1039/b905821b.PubMedView ArticleGoogle Scholar
- Roth BL, Sheffler DJ, Kroeze WK: Magic shotguns versus magic bullets: selectively non-selective drugs for mood disorders and schizophrenia. Nature Reviews Drug Discovery. 2004, 3 (4): 353-359. 10.1038/nrd1346.PubMedView ArticleGoogle Scholar
- Keith CT, Borisy AA, Stockwell BR: Multicomponent therapeutics for networked systems. Nature Reviews Drug Discovery. 2005, 4 (1): 71-U10. 10.1038/nrd1609.PubMedView ArticleGoogle Scholar
- Hopkins AL: Network pharmacology: the next paradigm in drug discovery. Nature Chemical Biology. 2008, 4 (11): 682-690. 10.1038/nchembio.118.PubMedView ArticleGoogle Scholar
- Zimmermann GR, Lehar J, Keith CT: Multi-target therapeutics: when the whole is greater than the sum of the parts. Drug Discovery Today. 2007, 12 (1-2): 34-42. 10.1016/j.drudis.2006.11.008.PubMedView ArticleGoogle Scholar
- Csermely P, Agoston V, Pongor S: The efficiency of multi-target drugs: the network approach might help drug design. Trends in Pharmacological Sciences. 2005, 26 (4): 178-182. 10.1016/j.tips.2005.02.007.PubMedView ArticleGoogle Scholar
- Korcsmaros T, Szalay MS, Csaba B, Kovacs IA, Csermely P: How to design multi-target drugs: target search options in cellular networks. Expert Opinion on Drug Discovery. 2007, 2 (6): 799-808. 10.1517/174604188.8.131.529.PubMedView ArticleGoogle Scholar
- Hopkins AL, Mason JS, Overington JP: Can we rationally design promiscuous drugs?. Current Opinion in Structural Biology. 2006, 16 (1): 127-136. 10.1016/j.sbi.2006.01.013.PubMedView ArticleGoogle Scholar
- Flordellis CS, Manolis AS, Paris H, Karabinis A: Rethinking target discovery in polygenic diseases. Current Topics in Medicinal Chemistry. 2006, 6 (16): 1791-1798. 10.2174/156802606778194226.PubMedView ArticleGoogle Scholar
- Dessalew N, Mikre W: On the paradigm shift towards multitarget selective drug design. Current Computer-Aided Drug Design. 2008, 4 (2): 76-90. 10.2174/157340908784533229.View ArticleGoogle Scholar
- Keskin O, Gursoy A, Ma B, Nussinov R: Towards drugs targeting multiple proteins in a systems biology approach. Current Topics in Medicinal Chemistry. 2007, 7 (10): 943-951. 10.2174/156802607780906690.PubMedView ArticleGoogle Scholar
- Morphy R: Selectively Nonselective Kinase Inhibition: Striking the Right Balance. Journal of Medicinal Chemistry. 2010, 53 (4): 1413-1437. 10.1021/jm901132v.PubMedView ArticleGoogle Scholar
- Sigrist CJA, Cerutti L, de Castro E, Langendijk-Genevaux PS, Bulliard V, Bairoch A, Hulo N: PROSITE, a protein domain database for functional characterization and annotation. Nucleic Acids Res. 2010, 38: D161-D166. 10.1093/nar/gkp885.PubMedPubMed CentralView ArticleGoogle Scholar
- Attwood TK, Bradley P, Flower DR, Gaulton A, Maudling N, Mitchell AL, Moulton G, Nordle A, Paine K, Taylor P, et al: PRINTS and its automatic supplement, prePRINTS. Nucleic Acids Res. 2003, 31 (1): 400-402. 10.1093/nar/gkg030.PubMedPubMed CentralView ArticleGoogle Scholar
- Pugalenthi G, Suganthan PN, Sowdhamini R, Chakrabarti S: MegaMotifBase: a database of structural motifs in protein families and superfamilies. Nucleic Acids Res. 2008, 36: D218-D221.PubMedPubMed CentralView ArticleGoogle Scholar
- Ku SY, Hu YJ: Structural alphabet motif discovery and a structural motif database. Computers in Biology and Medicine. 2012, 42 (1): 93-105. 10.1016/j.compbiomed.2011.10.013.PubMedView ArticleGoogle Scholar
- Weber A, Casini A, Heine A, Kuhn D, Supuran CT, Scozzafava A, Klebe G: Unexpected nanomolar inhibition of carbonic anhydrase by COX-2-selective celecoxib: New pharmacological opportunities due to related binding site recognition. Journal of Medicinal Chemistry. 2004, 47 (3): 550-557. 10.1021/jm030912m.PubMedView ArticleGoogle Scholar
- Kinnings SL, Liu N, Buchmeier N, Tonge PJ, Xie L, Bourne PE: Drug Discovery Using Chemical Systems Biology: Repositioning the Safe Medicine Comtan to Treat Multi-Drug and Extensively Drug Resistant Tuberculosis. Plos Computational Biology. 2009, 5 (7):Google Scholar
- Madsen D, Kleywegt GJ: Interactive motif and fold recognition in protein structures. J Appl Crystallogr. 2002, 35: 137-139. 10.1107/S0021889802000602.View ArticleGoogle Scholar
- Bauer RA, Bourne PE, Formella A, Frommel C, Gille C, Goede A, Guerler A, Hoppe A, Knapp EW, Poschel T, et al: Superimpose: a 3D structural superposition server. Nucleic Acids Res. 2008, 36: W47-W54. 10.1093/nar/gkn285.PubMedPubMed CentralView ArticleGoogle Scholar
- Debret G, Martel A, Cuniasse P: RASMOT-3D PRO: a 3D motif search webserver. Nucleic Acids Res. 2009, 37: W459-W464. 10.1093/nar/gkp304.PubMedPubMed CentralView ArticleGoogle Scholar
- Shulman-Peleg A, Shatsky M, Nussinov R, Wolfson HJ: MultiBind and MAPPIS: webservers for multiple alignment of protein 3D-binding sites and their interactions. Nucleic Acids Res. 2008, 36: W260-W264. 10.1093/nar/gkn185.PubMedPubMed CentralView ArticleGoogle Scholar
- Wu CY, Chen YC, Lim C: A structural-alphabet-based strategy for finding structural motifs across protein families. Nucleic Acids Res. 2010, 38 (14):Google Scholar
- Chiu YY, Lin CY, Lin CT, Hsu KC, Chang LZ, Yang JM: Space-related pharma-motifs for fast search of protein binding motifs and polypharmacological targets. Bmc Genomics. 2012, 13 (Suppl 7): S21-10.1186/1471-2164-13-S7-S21.PubMedPubMed CentralView ArticleGoogle Scholar
- Kabsch W, Sander C: Dictionary of Protein Secondary Structure - Pattern-Recognition of Hydrogen-Bonded and Geometrical Features. Biopolymers. 1983, 22 (12): 2577-2637. 10.1002/bip.360221211.PubMedView ArticleGoogle Scholar
- Rose PW, Beran B, Bi CX, Bluhm WF, Dimitropoulos D, Goodsell DS, Prlic A, Quesada M, Quinn GB, Westbrook JD, et al: The RCSB Protein Data Bank: redesigned web site and web services. Nucleic Acids Res. 2011, 39: D392-D401. 10.1093/nar/gkq1021.PubMedPubMed CentralView ArticleGoogle Scholar
- Yang JM, Tung CH: Protein structure database search and evolutionary classification. Nucleic Acids Res. 2006, 34 (13): 3646-3659. 10.1093/nar/gkl395.PubMedPubMed CentralView ArticleGoogle Scholar
- Tung CH, Huang JW, Yang JM: Kappa-alpha plot derived structural alphabet and BLOSUM-like substitution matrix for rapid search of protein structure database. Genome Biology. 2007, 8 (3):Google Scholar
- Holm L, Kaariainen S, Wilton C, Plewczynski D: Using Dali for structural comparison of proteins. Current Protocols in Bioinformatic. 2006, Chapter 5 (Unit 5): 5-Google Scholar
- Carhart RE, Smith DH, Venkataraghavan R: Atom Pairs as Molecular-Features in Structure Activity Studies - Definition and Applications. Journal of Chemical Information and Computer Sciences. 1985, 25 (2): 64-73.Google Scholar
- Notredame C, Higgins DG, Heringa J: T-Coffee: A novel method for fast and accurate multiple sequence alignment. J Mol Biol. 2000, 302 (1): 205-217. 10.1006/jmbi.2000.4042.PubMedView ArticleGoogle Scholar
- Hsu KC, Chen YF, Lin SR, Yang JM: iGEMDOCK: a graphical environment of enhancing GEMDOCK using pharmacological interactions and post-screening analysis. Bmc Bioinformatics. 2011, 12:Google Scholar
- Pilger BD, Perozzo R, Alber F, Wurth C, Folkers G, Scapozza L: Substrate diversity of herpes simplex virus thymidine kinase. Impact Of the kinematics of the enzyme. The Journal of biological chemistry. 1999, 274 (45): 31967-31973. 10.1074/jbc.274.45.31967.PubMedView ArticleGoogle Scholar
- Blume-Jensen P, Hunter T: Oncogenic kinase signalling. Nature. 2001, 411 (6835): 355-365. 10.1038/35077225.PubMedView ArticleGoogle Scholar
- Cohen P: Protein kinases - the major drug targets of the twenty-first century?. Nature Reviews Drug Discovery. 2002, 1 (4): 309-315. 10.1038/nrd773.PubMedView ArticleGoogle Scholar
- Noble MEM, Endicott JA, Johnson LN: Protein kinase inhibitors: Insights into drug design from structure. Science. 2004, 303 (5665): 1800-1805. 10.1126/science.1095920.PubMedView ArticleGoogle Scholar
- Weinmann H, Metternich R: Drug discovery process for kinase inhibitors. Chembiochem. 2005, 6 (3): 455-459. 10.1002/cbic.200500034.PubMedView ArticleGoogle Scholar
- Liao JJL: Molecular recognition of protein kinase binding pockets for design of potent and selective kinase inhibitors. Journal of Medicinal Chemistry. 2007, 50 (3): 409-424. 10.1021/jm0608107.PubMedView ArticleGoogle Scholar
- Liu Y, Gray NS: Rational design of inhibitors that bind to inactive kinase conformations. Nature Chemical Biology. 2006, 2 (7): 358-364. 10.1038/nchembio799.PubMedView ArticleGoogle Scholar
- Norman RA, Toader D, Ferguson AD: Structural approaches to obtain kinase selectivity. Trends in Pharmacological Sciences. 2012, 33 (5): 273-278. 10.1016/j.tips.2012.03.005.PubMedView ArticleGoogle Scholar
- Zhou J, Adams JA: Is there a catalytic base in the active site of cAMP-dependent protein kinase?. Biochemistry. 1997, 36 (10): 2977-2984. 10.1021/bi9619132.PubMedView ArticleGoogle Scholar
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