- Research article
- Open Access
RNA-seq reveals differentially expressed genes of rice (Oryza sativa) spikelet in response to temperature interacting with nitrogen at meiosis stage
© Yang et al. 2015
- Received: 21 March 2015
- Accepted: 23 October 2015
- Published: 17 November 2015
Rice (Oryza sativa) is one of the most important cereal crops, providing food for more than half of the world’s population. However, grain yields are challenged by various abiotic stresses such as drought, fertilizer, heat, and their interaction. Rice at reproductive stage is much more sensitive to environmental temperatures, and little is known about molecular mechanisms of rice spikelet in response to high temperature interacting with nitrogen (N).
Here we reported the transcriptional profiling analysis of rice spikelet at meiosis stage using RNA sequencing (RNA-seq) as an attempt to gain insights into molecular events associated with temperature and nitrogen. This study received four treatments: 1) NN: normal nitrogen level (165 kg ha−1) with natural temperature (30 °C); 2) HH: high nitrogen level (330 kg ha−1) with high temperature (37 °C); 3) NH: normal nitrogen level and high temperature; and 4) HN: high nitrogen level and natural temperature, respectively. The de novo assembly generated 52,553,536 clean reads aligned with 72,667 unigenes. About 10 M reads were identified from each treatment. In these differentially expressed genes (DEGs), we found 151 and 323 temperature-responsive DEGs in NN-vs-NH and HN-vs-HH, and 114 DEGs were co-expressed. Meanwhile, 203 and 144 nitrogen-responsive DEGs were focused in NN-vs-HN and NH-vs-HH, and 111 DEGs were co-expressed. The temperature-responsive genes were principally associated with calcium-dependent protein, cytochrome, flavonoid, heat shock protein, peroxidase, ubiquitin, and transcription factor while the nitrogen-responsive genes were mainly involved in glutamine synthetase, transcription factor, anthocyanin, amino acid transporter, leucine zipper protein, and hormone. It is noted that, rice spikelet fertility was significantly decreased under high temperature, but it was more reduced under higher nitrogen. Accordingly, numerous spikelet genes involved in pollen development, pollen tube growth, pollen germination, especially sporopollenin biosynthetic process, and pollen exine formation were mainly down-regulated under high temperature. Moreover, the expression levels of co-expressed DEGs including 5 sporopollenin biosynthetic process and 7 pollen exine formation genes of NN-vs-NH were lower than that of HN-vs-HH. Therefore, these spikelet genes may play important roles in response to high temperature with high nitrogen and may be good candidates for crop improvement.
This RNA-seq study will help elucidate the molecular mechanisms of rice spikelet defense response to high temperature interacting with high nitrogen level.
- De novo assembly
- Differentially expressed genes
- High temperature
- Spikelet fertility
Rice (Oryza sativa L.) is the staple food for more than half of the world’s population, and a continuous increase in rice production is needed to meet the growing food demand resulted from population growth in the future . In the recent hundred of years, rice is increasingly cultivated in more marginal environmental stresses because of global warming, and heat stress is a major abiotic limitation to plant growth and development . The Intergovernmental Panel on Climate Change (IPCC) predicted that the vulnerability of the crop will be increased with a projected global average surface temperature increase by 0.74 ± 0.18 °C . In these environments, the uncontrollable temperature frequently exceed the critical temperature of seed set, resulting in spikelet sterility and reduced yield [4, 5]. It was estimated that rice grain yields decline by 10 % for each 1 °C increase in minimum temperature . In addition to the temperature, the controllable nitrogen (N) is another factor affecting rice yield. In the past, increased rice production was largely attributed to the elevated application of nitrogen . Moreover, grain yields were challenged by high temperature interacting with other abiotic stresses [8, 9]. But few reports are demonstrated the effects of high temperature interacting with nitrogen on rice production at meiosis stage.
High temperature beyond the critical threshold at reproductive stage leads to spikelet sterility. Morphological observations revealed that reduced spikelet fertility was mainly due to poor anther dehiscence, low pollen production and pollen viability, and reduced germination rate on the rice stigma under high temperature [4, 5, 10, 11]. The recent studies about the molecular biology of spikelet fertility had shown that many differentially expressed genes (DEGs) played an essential role in heat response. It was reported that high temperature treatment (39 °C) at the microspore stage reduced spikelet fertility, DNA microarray analysis revealed that at least 13 genes were designated as high temperature-repressed genes in the anther, and these genes expressed specifically in the immature anther were mainly in the tapetum at the microspore stage and down-regulated after 1 d of high temperature . Zhang et al.  provided a gene expression profile of rice panicle grown under 40 °C for different durations (0 min, 20 min, 60 min, 2 h, 4 h, and 8 h) during anther development by using a rice microarray, and found that the identified differentially expressed genes were mainly involved in transcriptional regulation, transport, cellular homeostasis, and stress response. Furthermore, the time-dependent gene expression pattern was discovered under heat stress, and the regulation model central to reactive oxygen species (ROS) combined with transcriptome and ROS quantification data in rice panicle indicated the great importance to maintain ROS balance and the existence of wide cross-talk in heat response in their study. It has been reported that 50.4 % of all genes in the rice genome (28,296/56,143) were expressed at 25 °C, and a similar number of genes (50.2 %; 28,189/56,143) were expressed in rice grown under 30 °C. Furthermore, the temperature markedly stimulated several super-families of transcription factors, including bZIP, MYB, and WRKY . Thus far, little is known about the transcriptional mechanism of rice spikelet in response to heat at meiosis stage.
Nitrogen is an essential components of various macromolecules, such as proteins, nucleic acids, many cofactors, and some plant hormones . Nitrogen affects many aspects of plant function, from metabolism to resource allocation, growth, and development [15, 16]. In order to meet the high nitrogen requirement of plant growth and crop production, a large quantity of nitrogen fertilizers are applied [14, 17]. However, crop plants only use less than half of the applied nitrogen fertilizers , and the unused nitrogen (nitrogen pollution) is inevitably becoming a threat to global environment [19, 20]. Furthermore, the more application of nitrogen fertilizers has markedly increased the cost in crop production, which greatly affects the income of the farmers. Thus, it is extremely valuable to develop strategies that crops are less dependent on the heavy application of nitrogen fertilizers to create a sustainable and efficient agricultural system while maintaining high yield. Efforts have been directed to understanding the molecular basis of plant responses to nitrogen, and many nitrogen-responsive genes were identified . Ammonium (NH4 +) and nitrate (NO3 −) are two primary inorganic nitrogen sources available for plants. Nitrate and ammonium are absorbed by the plant via a variety of transporters that are divided into high-affinity transport systems (HATS) and low-affinity transport systems (LATS) [15, 16]. Nitrate is taken up by the low and high affinity nitrate transporter gene family members (NRT1 and NRT2), reduced to nitrite by nitrate reductase (NR), and to ammonium by nitrite reductase (NiR) . It is well known that the members of AMT1 gene family encoded the HATS for ammonium . Ammonium is incorporated into organic molecules, catalyzed primarily by glutamine synthetase (GS) and glutamate synthase (GOGAT) pathway . At the molecular level, nitrogen limitation altered the expression levels of 629 genes with 340 of them up-regulated and 289 of them down-regulated in Arabidopsis, and numerous nitrogen-responsive genes encoded transcription factors, signal transduction components, and proteins required for hormone synthesis and response . It was reported that 10,422 genes at an early stage of low nitrogen stress in rice seedling were identified . Wang et al.  studied the response of seedlings grown on ammonium to the addition of low or high levels of nitrate, and identified 25 and 49 nitrogen-responsive genes to low or high nitrate induction, respectively. These studies have provided valuable insights into nitrogen response and its linkage to other biological pathways. However, knowledge about plant genes and pathways in response to nitrogen is still lacking, while such knowledge is essential for formulating strategies for manipulating the genetic architecture of the plants to improve the nitrogen use efficiency.
The combination of nitrogen and temperature affects plant growth and development. Weng at al  examined the differences between photosynthetic activity and dark respiratory rate as influenced by leaf nitrogen levels and temperatures in rice, and found the photosynthetic rates and respiratory rate were correlated with the leaf nitrogen content. Liu et al.  evaluated mycorrhizal rice growth based on treatments at two temperatures (15 °C and 25 °C) and two nitrogen levels (20 mg pot−1 and 50 mg pot−1). The arbuscular mycorrhizal fungi (AMF) colonization of rice resulted in different responses of the plants to low and high nitrogen levels. Satake et al.  showed that high nitrogen level from the spikelet differentiation stage to the young microspore stage greatly increased the sensitivity to coolness at the critical stage in rice plants. Hayashi  showed that cool temperature (12 °C) for 3 days decreased rice spikelet fertility by 36 % under standard-nitrogen and 42 % under high-nitrogen conditions. But there are no reports about the effects of the nitrogen interacting with high temperature on spikelet fertility in rice production.
Recently, RNA sequencing technology, based on next-generation sequencing technology, provides a platform for measuring differences in gene expression . Unlike microarrays, RNA-seq does not require prior knowledge of gene sequences. Furthermore, RNA-seq provides a far more precise measurement of transcripts and has been successfully used for transcript profiling in many plant species [33, 34]. Seed setting rate, one of the most important components of rice grain yields, is significantly affected by high temperature during pollen mother cell meiosis. However, limited information about differentially expressed genes of rice spikelet in response to high temperature is available at meiosis stage. Moreover, no reports demonstrate that nitrogen level can regulate the effects of high temperature at meiosis stage on rice production. In this study, we used Illumina RNA-seq technology to profile different gene expression of rice spikelet from a conventional strain Zhong531 treated by high temperature and high nitrogen level at meiosis stage. The overall objective of this study was to increase our understanding of the heat response in rice spikelet and provided good candidate genes for crop improvement.
One rice (Oryza sativa L. ssp. indica) strain, Zhong531, was chosen for this study. The trials were conducted between March and August in 2014, in net house at High-Tech Agricultural Science and Technology Park of Jiangxi Agricultural University (latitude: 28° 46′ N, longitude: 115° 50′ E, altitude: 48.80 m), Nanchang, Jiangxi Province, China. Seed dormancy was broken by exposure to the sun for 3 d, followed by pre-germination and sown in a rice field. Thirty-day-old seedlings were then transplanted on 25 April at a spacing of 22.5 cm × 24.5 cm with only one seedling per hill. The physical and chemical properties of experimental soil were as follows: soil pH, organic matter, total nitrogen, available nitrogen, available phosphorous (P), and available potassium (K) were 5.94, 28.72 g kg−1, 1.45 g kg−1, 92.01 mg kg−1, 28.31 mg kg−1, and 221.67 mg kg−1, respectively. The experimental received four treatments: 1) NN: normal nitrogen level (165 kg N ha−1, as the control) with natural temperature (30 °C, as the control); 2) HH: high nitrogen level (330 kg N ha−1) with high temperature (37 °C); 3) NH: normal nitrogen level and high temperature; and 4) HN: high nitrogen level and natural temperature, respectively. The amount of nitrogen fertilizer in the form of urea for the normal nitrogen level was recommended by local agricultural extension employees based on experience and target yield. For normal nitrogen level, 66, 33, and 66 kg N ha−1 (as pure N) were applied at basal (2 d before transplanting), tillering (10 d after transplanting), and panicle initiation (30 d after transplanting), respectively. For high nitrogen level, 66, 66, and 198 kg N ha−1 were applied at basal, tillering, and panicle initiation, respectively . Phosphorus (90 kg P ha−1 as P2O5) was applied 2 d before transplanting. Potassium (180 kg K ha−1 as K2O) was applied in two equal splits at basal and panicle initiation. Weeds in the field were manually removed at early growth stages. Insecticides were used to prevent insect damage. All other agronomic practices referred to the local recommendations to avoid yield loss.
High temperature treatment
Four temperature-controlled growth cabinets were specifically designed to study the impacts of the high temperature (37 °C) and natural temperature (30 °C), respectively. Each cabinet (1.91 m × 0.76 m × 1.83 m in length, width and height, respectively) was fixed at a 5 m interval to ensure adequate ventilation. For high temperature analysis, rice plants along with soil at the female stamen primordium differentiation stage were randomly transplanted into plastic pots to adapt for 3 d and then transferred to growth cabinets. The pot with a hole at the bottom has internal diameter of 17 cm and height of 16 cm, and the rice plants transferred into pots grew normally and stably without withered and yellow leaves. Twelve rice plants with each in a pot for each treatment combination at the formation stage of pollen mother cell  were randomly exposed to high temperature (from 8:00 o'clock to 18:00 o'clock, Beijing time, 37 °C; from 18:00 o'clock to the next morning 8:00 o'clock, 30 °C), and natural temperature (from 8:00 o'clock to 18:00 o'clock, 30 °C; from 18:00 o'clock to the next morning 8:00 o'clock, 25 °C) for consecutive 4 d, respectively . Two stand-alone sensors were placed in the rice canopy near the panicle base (few centimeters into the canopy) in each cabinet to measure temperature once 15-min interval, with all the sensors connected to data loggers (HOBO, U22–001, USA) [36, 37]. The other controlled environmental conditions (white fluorescent illumination of 540 μmol m−2 s−1 day/ 0 μmol m−2 s−1 night, and relative humidity of 75 % day/ 80 % night) in each growth cabinet were consistent. The four treatments of combining temperature and nitrogen were in two repeats, each repeat with six plants (pots) per growth cabinet. No cabinet or replicate effects were observed, therefore, data collected from different replicates and cabinets for each treatment were pooled. According to the descriptions by Endo et al. , Tang et al. , and Chen et al. , we collected young florets in the same phases of the meiosis stage as samples for further analysis. Features used to distinguish this stage are as follows: a) rice was grown for about 2 months, 13 to19 days before flowering; b) the distance between the auricle of the flag leaf and that of the penultimate leaf was within 1 cm; c) the young panicle length was about 2 cm; d) the floret length was 2 to 3 mm. Young florets during the meiosis of pollen mother cell at the middle of main panicles after 4 d in the high temperature environments were collected, frozen in liquid nitrogen immediately, and stored at −80 °C for further use. Each sample represented two replicates (each replicate had 3 plants).
After high temperature, the pots were removed and the remaining 6 undamaged rice plants were returned to natural field conditions until seed maturity. The average temperatures in the cabinets were close to the required targets: high temperature (from 8:00 o'clock to 18:00 o'clock, actual, 37.08 °C, SE = ±0.13 °C; from 18:00 o'clock to the next morning 8:00 o'clock, 29.71 ± 0.05 °C), and natural temperature (from 8:00 o'clock to 18:00 o'clock, 29.46 ± 0.11 °C; from 18:00 o'clock to the next morning 8:00 o'clock, 26.07 ± 0.13 °C), respectively.
Spikelet fertility (seed-set)
Spikelet fertility (seed-set) was estimated according to the procedures of Prasad et al. . At physiological maturity stage, 16 randomly selected main tiller panicles (four each from separate plant) were tagged and harvested in each treatment. Spikelet fertility was estimated as the ratio of the number of filled grains to the total number of reproductive sites (florets) and expressed as percentage. Each floret was pressed between the forefinger and thumb to determine if the grain was filled or not. Number of filled grains included both completely and partially filled grains.
Differences among the means of spikelet fertility in the four treatments were analyzed using Student’s t-test (SPSS statistical software ver. 17.0). The standard errors of the mean were also calculated and presented in the graphs as error bars.
cDNA preparation for Illumina sequencing
Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions. The total RNA samples from the four treatments were mixed and pooled (with equal amount of RNA from each treatment) as one sample for transcriptome sequencing (paired ends sequencing, 90 bp) to obtain as much gene expression information as possible, but they were subjected individually to conduct digital gene expression (DGE) sequencing. The total RNA samples were first treated with deoxyribonuclease I (DNase I) to degrade any possible DNA contamination. Then the mRNA was enriched by using the Oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA was fragmented into short fragments (about 200 bp). Then the first strand of cDNA was synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second strand. The double strand cDNA was purified with magnetic beads. End reparation and 3-end single nucleotide A (adenine) addition was then performed. Finally, sequencing adaptors were ligated to the fragments. The fragments were enriched by PCR amplification. During the quality control (QC) step, Agilent 2100 Bioanaylzer, and ABI StepOnePlus Real-Time PCR System were used to qualify and quantify of the sample library. The library products were ready for sequencing via Illumina HiSeq™ 2000 at the Beijing Genomics Institute (BGI, http://www.genomics.cn/index; Shenzhen, China) (Additional file 1: Figure S1). Image data output from sequencing machine were transformed by base calling into sequence data, called raw data or raw reads, and was stored in fastq format.
Data filtering and de novo assembly
Raw reads produced from sequencing machines contained dirty reads which contain adapters, unknown or low quality bases. These data would negatively affect following bioinformatics analysis. After removal of adaptor sequences along with low quality reads (the rate of reads which quality value < = 10 is more than 20 %), and reads of larger than 5 % unknown sequences (reads with unknown sequences ‘N’), the resting clean reads were assembled into unigenes by Trinity , a short-read assembly program.
Transcriptome de novo assembly was carried out with a short reads assembling program–Trinity. Trinity combined three independent software modules: Inchworm, Chrysalis, and Butterfly, was applied sequentially to process large volumes of RNA-seq reads. The Trinity software first combined reads with a certain length of overlap to form longer fragments without N, forming contigs. Then the reads were mapped back to contigs, with paired-end reads it was able to detect contigs from the same transcript as well as the distances between these contigs. Then, Trinity connected these contigs to get consensus sequences that contained the least N and could not be extended on either end. Such sequences were defined as unigenes. When multiple samples from the same species were sequenced, unigenes from each sample’s assembly can be taken into further process of sequence splicing and redundancy removing with sequence clustering software to acquire non-redundant unigenes as long as possible. Then gene family clustering was done. The unigenes would be divided to two classes. One was clusters, which the prefix was CL and the cluster ID was behind. In the same cluster, there were several unigenes with the similarity between them was more than 70 %. And the other was singletons, which the prefix was unigene.
In the final step, blastx alignment (evalue < 0.00001) between unigenes and protein databases like NR (NCBI non-redundant protein sequences), Swiss-Prot (A manually annotated and reviewed protein sequence database), KEGG (Kyoto Encyclopedia of Genes and Genomes) and COG (Cluster of Orthologous Groups of proteins) was performed, and the best aligning results were used to decide sequence direction of unigenes. If results of different databases conflicted with each other, a priority order of NR, Swiss-Prot, KEGG and COG should be followed when deciding sequence direction of unigenes. When a unigene happened to be unaligned to any of the above four databases, the software named ESTScan  would be introduced to decide its sequence direction. For unigenes with sequence directions, we provided their sequences from 5' end to 3' end; for those without any direction we provided their sequences from the assembly software (Additional file 2: Figure S2).
Statistics of digital gene expression (DGE) sequencing
After DGE sequencing, clean reads were mapped to reference genes (de novo assembly) and/or reference genome (Indica rice database) using SOAPaligner/ SOAP2 . No more than 2 mismatches were allowed in the alignment. The distribution of reads on the reference genes was used to evaluate the randomness . Genes with similar expression patterns usually indicated functional correlation. We performed a cluster analysis of gene expression patterns with cluster software  and Java Treeview software . Further evaluation included quality assessment of reads, and sequencing saturation analysis, gene sequencing coverage, correlation analysis of all genes between every two samples replicates.
Functional unigene annotation and COG classification
Unigene annotation provides information of expression and functional annotation of a unigene. Information of functional annotation gives protein functional annotation, COG functional annotation and Gene Ontology (GO) functional annotation of unigenes. Unigene sequences were firstly aligned to protein databases like NR, Swiss-Prot, KEGG and COG (e-value < 0.00001) by BLASTX, and NCBI nucleotide sequences database (NT) (e-value < 0.00001) by BLASTN, retrieving proteins with the highest sequence similarity with the given unigenes along with their protein functional annotations (Additional file 3: Figure S3).
Where N is the number of all genes with GO annotation; n is the number of DEGs in N; M is the number of all genes that are annotated to the certain GO terms; m is the number of DEGs in M. The calculated P-value goes through Bonferroni Correction, taking corrected P-value < = 0.05 as a threshold. GO terms fulfilling this condition are defined as significantly enriched GO terms in DEGs.
KEGG (Kyoto Encyclopedia of Genes and Genomes) is the major public pathway-related database [47, 48]. Pathway enrichment analysis identifies significantly enriched metabolic pathways or signal transduction pathways in DEGs comparing with the whole genome background. The calculating formula was the same as that in GO analysis.
COG (Cluster of Orthologous Groups of proteins) is a database which classifies orthologous gene product, each COG protein is presumed coming from ancestral protein, the construction of COG database is based on the complete genome encoding protein and phylogenetic relationships of bacteria, algae, eucaryon. We mapped the unigene to the COG database, predicted the possible functions and statistics, understand gene function distribution characteristics of the species from the macro .
Screening of differentially expressed genes (DEGs)
Here RPKM (A) is the expression level of gene A, C is number of reads that uniquely aligned to gene A, N is total number of reads that uniquely aligned to all genes, and L is number of bases of gene A. The RPKM method is able to eliminate the influence of different gene length and sequencing discrepancy on the calculation of gene expression level. Therefore, the RPKM values can be directly used for comparing the difference of gene expression among samples.
DEGs analysis included the screening of genes that were differentially expressed among samples, and GO functional enrichment analysis and KEGG pathway enrichment analysis for these DEGs. Referring to the method of Audic and Claverie , we had developed a strict algorithm to identify DEGs between two samples. P-value corresponded to differential gene expression test. FDR (False Discovery Rate) is a method to determine the threshold of P-value in multiple tests. Assume that we had picked out R differentially expressed genes in which S genes really showed differential expression and the other V genes were false positive. If we decided that the error ratio Q = V / R must stay below a cutoff (<1 %), we should preset the FDR to a number no larger than 0.01 . We used FDR ≤0.001 and the absolute value of log2 (Ratio) ≥1 as the threshold to judge the significance of gene expression difference. More stringent criteria with smaller FDR and bigger fold-change value can be used to identify DEGs (Additional file 4: Figure S4).
Illumina paired-end sequencing and de novo assembly of transcriptome
Output statistics of transcriptome sequencing
Total raw reads
Total clean reads
Total clean nucleotides (bp)
Total number of contigs
Total length of contigs (bp)
Mean length of contigs (bp)
N50 of contigs (bp)
Total number of unigenes (bp)
Total length of unigenes (bp)
Mean length of unigenes (bp)
N50 of unigenes (bp)
Functional annotation and classification of unigenes
Statistics of annotation results
Total number of annotated unigenes
Percentage of annotated unigenes (%)
In order to annotate the transcriptome, a total of 72,667 unigenes were first examined against the NR database in NCBI using BLASTX with an E-value cut-off of 1e−5, which showed 58,879 (81.03 %) having significant BLAST hits (Table 2). The E-value distribution of significant hits revealed that 25,886 (43.97 %) of matched unigenes had strong homology (smaller than 1.0e-60), while the other homologous 32,993 (56.03 %) unigenes had E-values in the range of 1.0e-60 to 1.0e-5 (Additional file 5: Figure S5 (A)). The distribution of unigenes similarities presented that most of the BLASTX hits (48,515; 82.40 %) were higher than 95 %. Only 10,364 (17.60 %) of hits had sequence similarity values less than 95 % (Additional file 5: Figure S5 (B)). The species distribution revealed that 35,404 (60.13 %) unigenes had homology to rice (Oryza sativa L. ssp. japonica) sequences. Only 20,544 (34.89 %) unigenes had strong homology to rice (Oryza sativa L. ssp. indica) sequences (Additional file 5: Figure S5 (C)).
KEGG pathway classification of transcriptome (the top 10)
All genes with pathway annotation (31,242)
8,736 (27.96 %)
4,026 (12.89 %)
mRNA surveillance pathway
3,569 (11.42 %)
3,145 (10.07 %)
3,115 (9.97 %)
Biosynthesis of secondary metabolites
3,074 (9.84 %)
Ether lipid metabolism
2,924 (9.36 %)
1,760 (5.63 %)
1,405 (4.5 %)
Plant hormone signal transduction
1,365 (4.37 %)
Digital gene expression library sequencing (DGE sequencing)
Summary of reads mapped to reference genes (de novo assembly of transcriptome)
Total base pairs
<=2 bp Mismatch
Total unmapped reads
12,532,019 (100.00 %)
614,068,931 (100.00 %)
10,688,571 (85.29 %)
9,100,428 (72.62 %)
1,588,143 (12.67 %)
9,065,210 (72.34 %)
1,623,361 (12.95 %)
1,843,448 (14.71 %)
11,933,716 (100.00 %)
584,752,084 (100.00 %)
10,130,167 (84.89 %)
8,669,541 (72.65 %)
1,460,626 (12.24 %)
8,601,124 (72.07 %)
1,529,043 (12.81 %)
1,803,549 (15.11 %)
12,029,191 (100.00 %)
589,430,359 (100.00 %)
10,194,826 (84.75 %)
8,731,597 (72.59 %)
1,463,229 (12.16 %)
8,703,059 (72.35 %)
1,491,767 (12.40 %)
1,834,365 (15.25 %)
12,381,854 (100.00 %)
606,710,846 (100.00 %)
10,448,097 (84.38 %)
8,879,274 (71.71 %)
1,568,823 (12.67 %)
8,891,358 (71.81 %)
1,556,739 (12.57 %)
1,933,757 (15.62 %)
12,404,809 (100.00 %)
607,835,641 (100.00 %)
10,518,064 (84.79 %)
9,018,788 (72.70 %)
1,499,276 (12.09 %)
8,850,183 (71.34 %)
1,667,881 (13.45 %)
1,886,745 (15.21 %)
12,057,086 (100.00 %)
590,797,214 (100.00 %)
10,235,897 (84.90 %)
8,758,708 (72.64 %)
1,477,189 (12.25 %)
8,619,541 (71.49 %)
1,616,356 (13.41 %)
1,821,189 (15.10 %)
12,245,417 (100.00 %)
600,025,433 (100.00 %)
10,454,895 (85.38 %)
8,914,987 (72.80 %)
1,539,908 (12.58 %)
8,797,125 (71.84 %)
1,657,770 (13.54 %)
1,790,522 (14.62 %)
11,936,315 (100.00 %)
584,879,435 (100.00 %)
10,150,654 (85.04 %)
8,632,637 (72.32 %)
1,518,017 (12.72 %)
8,601,628 (72.06 %)
1,549,026 (12.98 %)
1,785,661 (14.96 %)
Assessment of DGE sequencing
Sequence saturation analysis is used to measure the sequencing data of a sample. With the number of reads increasing, the number of detected genes is increasing. However, when the number of reads reaches a certain amount, the growth curve of detected genes flattens, which indicates that the number of detected genes has a tendency to saturation. As the Additional file 9: Figure S7 shows, when the sequencing amount of the four DGE libraries reached nearly 10 M, the number of detected genes almost ceased to increase.
During the RNA-Seq experiment, mRNA were first broken into short segments by chemical method and then sequenced. If the randomness is poor, reads preference to specific gene region will directly affect subsequent bioinformatics analysis. We used the distribution of reads on the reference genes to evaluate the randomness. Since reference genes have different lengths, the reads position on gene is standardized to a relative position (which is calculated as the ratio between reads location position on the gene and gene length), and then the number of reads in each position is counted. Additional file 10: Figure S8 was the result showing the distribution of reads on the reference genes of all four samples, which indicated that the randomness was good, and the reads in every position were evenly distributed.
Gene coverage is the percentage of a gene covered by reads. This value is determined as the ratio of the base number in a gene covered by unique mapping reads to the total bases number of that gene. The percentage of gene coverage above 90 % for all samples was about 40 % (Additional file 11: Figure S9). The expression level of each gene is determined by the numbers of reads uniquely mapped to the specific gene and the total number of uniquely mapped reads in the sample (RPKM). The summary results of gene expression and related information for all examples were given in Additional file 12: Table S3.
Good performance of screening group differentially expression genes needs a high correlation among the same replicates. So we used Pearson method to get coefficient of all genes between every two samples just for reference. The coefficient of all genes between every two samples was about 90 %, which indicated the performance of two replicates was excellent (Additional file 13: Figure S10).
Differentially expressed genes (DEGs) among all samples
To understand the functions of these differentially expressed genes, all the DEGs were mapped to terms in the GO database and compared to the whole transcriptome background. The DEGs had a GO ID and can be categorized into small functional groups in three main categories (biological process, cellular component, and molecular function) of the GO classification (Additional file 18: Table S8, Additional file 19: Figure S11). Based on sequence homology, the following significant DEGs annotated by the GO database were identified: (a) in ZNN-vs-ZNH and ZHN-vs-ZHH, 50 and 51 functional groups were categorized, respectively; (b) in ZNN-vs-ZHN and ZNH-vs-ZHH, 55 and 44 functional groups were categorized, respectively. Among these groups, “metabolic process” and “cellular process” were dominant within the “biological process” category, the “cell” and “cell part” categories were dominant in the “cellular component” category, and “catalytic activity” and “binding” were dominant in the “molecular function” category (Additional file 19: Figure S11).
Effects of nitrogen level and high temperature at late spikelet differentiation stage (meiosis) on spikelets fertility of rice
In recent years, RNA-seq has been used as a powerful and cost-efficient tool for mining gene resources and functions. In this study, we performed de novo assembly of transcriptome in rice spikelet using the platform of Illumina Hiseq 2000, and obtained a total 4.73 Gb of transcriptome data. In the results of assembly, 72,667 unigenes were detected, with the total length 39,029,083 nt, average length 537 nt, and N50 747 nt. For functional annotation analysis, unigenes were annotated with the databases of NR (58,879), NT (72,255), Swiss-Prot (34,661), KEGG (31,242), COG (18,255), and GO (43,180) by using BLASTX with a cutoff e-value of 10−5, respectively, and the total annotated unigenes were 72,436. We obtained over 10 million clean reads from the samples of four digital gene expression profiling libraries (NH, NN, HH, HN), respectively. A lot of up-regulated and down-regulated genes were differentially expressed among the four libraries. The DEGs data provided comprehensive information of the gene expression in rice spikelet, facilitating our understanding of the molecular mechanisms of the different physiological aspects of rice spikelet in response to temperature interacting with nitrogen at meiosis stage.
Rice growth is challenged by fluctuations in environmental factors specially temperature on almost daily basis. To minimize high temperature damage, rice has developed adaptations and heat tolerance during evolution through the regulation of gene expression and changes in cellular processes. It’s well known that heat shock proteins (HSPs) are responsive to heat stress in plants [53–55]. In plants, HSPs are encoded by nuclear multigene families and localized in different cellular compartments. HSPs generally function as molecular chaperones, and are divided into HSP100, HSP90, HSP70, HSP60, HSP40 and HSP20 or small heat shock proteins (sHSPs) [56, 57]. When plants are exposed to elevated temperature, the protective HSPs have increased levels of protein and gene expression [58–61]. AtHSP70-15-deficient plants under heat stress resulted in drastically increase in mortality, indicating that AtHSP70-15 played an essential role during normal growth and in the heat response of Arabidopsis . A heat-tolerant rice N22 with 71 % spikelet fertility had a cold (putative) and a heat (unknown) shock protein significantly up-regulated under 38 °C during anthesis, indicating that the shock proteins may have a greater contribution to the heat tolerance for N22 . Our results revealed that HSP90, HSP70 and HSP20 in these differentially expressed genes (DEGs) were positively responsive to high temperature (Additional file 15: Table S5). Thus, the Hsps genes play an important part in response to heat stress in rice spikelet. Moreover, there were 19 and 26 DEGs annotated as HSPs in between ZNN-vs-ZNH and ZHN-vs-ZHH (Additional file 15: Table S5), respectively, and 18 HSPs occurred simultaneously in the two comparisons (Additional file 17: Table S7). In these DEGs, the gene expression of CL3468.Contig1, Unigene9201, Unigene5315, Unigene5314, and Unigene25510 in ZNN-vs-ZNH was extremely significant with log2Ratio ≥ 4.0, and CL3468.Contig1, Unigene5315, Unigene5314 in ZHN-vs-ZHH was extremely significant with log2Ratio ≥ 5.0. Manwhile, the log2Ratio value of Unigene9201, Unigene47969, Unigene24821, Unigene24300, Unigene41142, Unigene41141, Unigene41143, and Unigene25395 of ZHN-vs-ZHH was greater compared to ZNN-vs-ZNH, and the gene expression of other 7 HSP genes was on the contrary. These results may explain the gene expression difference of rice spikelet in response to high temperature under two different nitrogen levels.
The cytochrome P450, ubiquitin protein, flavonoid, and transcription factor genes have been extensively studied in plants in response to heat stresses [12, 63, 64]. Most of the heat-responsive (P450 family) genes were repressed to some extent, and only 10 P450 family genes were up-regulated under 40 °C at the stage 8 of anther development . High temperature reduced spikelet fertility of rice, DNA microarray analysis revealed that cytochrome P450 family genes were designated as high temperature-repressed genes in the anther at the microspore stage . In this study, we found that 17 cytochrome P450 genes were all down-regulated in ZNN-vs-ZNH, but 14 and 8 cytochrome P450 genes were up-regulated and down-regulated in ZHN-vs-ZHH, respectively (Additional file 15: Table S5). Furthermore, 6 co-expressed cytochrome P450 genes (Unigene7325, Unigene42477, Unigene42476, Unigene45111, CL2270.Contig1, and Unigene42480) showed different expression in two comparisons with all genes down-regulated in ZNN-vs-ZNH and 3 genes up-regulated in ZHN-vs-ZHH (Additional file 17: Table S7). Besides, the genes belonging to ubiquitin protein, flavonoid, peroxidase, and transcription factor also showed expression differences between ZNN-vs-ZNH and ZHN-vs-ZHH.
Nitrogen is one of the major macronutrients for higher plants and can be served as a signal molecule to regulate plant development, physiology, and metabolism [14, 65]. Peng et al.  found that nitrogen limitation altered the expression levels of 629 genes with 340 of them up-regulated and 289 of them down-regulated in Arabidopsis. The up-regulated group included the genes involved in protein degradation and the biosynthesis of anthocyanin, while the down-regulated group contained the genes functioning in photosynthesis and the synthesis of nitrogenous macromolecules such as chlorophyll, proteins, amino acids and nucleotides. Plant hormone play many biological roles in plants. Hirano et al.  analyzed the global expression profiles of genes related to phytohormones in microspore/pollen (MS/POL) development. The genes required for IAA and gibberellin (GA) synthesis were coordinately expressed during the later MS/POL developmental stage. In contrast, genes for GA signaling were preferentially expressed during the early stage. Phenotypic analysis revealed that the GA-deficient mutant reduced pollen elongation was defective in pollen tube elongation, resulting in a low spikelet fertilization frequency, whereas the GA-insensitive semidominant mutant was mainly defective in viable pollen production. Furthermore, the GA biosynthesis genes were preferentially expressed after meiosis during pollen development . It was reported that reduction of gibberellin by low temperature (approximately 19 °C) disrupted pollen development and caused severe reduction of seed setting in rice . It was reported that nitrogen responsive genes encoded transcription factors, signal transduction process, and proteins required for plant hormone synthesis and response [69–71]. In this study, we found 203 and 144 nitrogen-responsive DEGs in ZNN-vs-ZHN and ZNH-vs-ZHH, respectively (Additional file 15: Table S5). Numerous nitrogen responsive genes encoded plant hormone synthesis and response such as abscisic acid, auxin, gibberellin, cytokinin, and ethylene. Furthermore, a total of 111 DEGs associated with nitrogen co-expressed (Additional file 17: Table S7). WRKY genes encode transcription factors with a WRKY domain that belongs to zinc-finger proteins, which play important roles in responses to abiotic stress in rice [72, 73]. In these co-expressed DEGs, 8 WRKY transcription factor genes were all down-regulated, and the log2Ratio value of Unigene20481, CL4288.Contig1, CL554.Contig1, CL4639.Contig1, CL1348.Contig1, and Unigene16792 of ZNN-vs-ZHN was greater than that of ZNH-vs-ZHH. High temperature cause comprehensive alterations in transcription, but application of auxin can block the transcriptional alterations, leading to the production of normal pollen grains, and the normal seed setting rate under increasing temperatures . We found that 9 auxin-regulated genes with 4 down-regulated and 5 up-regulated DEGs were co-expressed. Our results revealed the gene expression difference of rice spikelet in response to nitrogen.
High temperature during flowering (anthesis and fertilization) greatly reduces spikelet fertility in rice . Pollen viability in rice plants exposed to (39 °C) was lower than that in control plants, and the pollen grains were very poorly attached and displayed limited germination on the stigma under high temperature, leading to reduced spikelet fertility. Spikelet fertility is closely related to pollen exine formation, pollen tube growth, pollen germination, and pollen development in plants. An ABC transporter gene (OsABCG15) was identified to be involved in pollen development in rice. Wu et al.  showed that OsABCG15 played an essential role in the formation of the rice anther cuticle and pollen exine. Ling et al.  identified 291 mature anther-preferentially expressed genes (OsSTA) in rice, and OsSTA genes were associated with pollen fertility, pollen germination and anther dehiscence in rice. The pollen semi-sterility1 (PSS1) encoded a kinesin-1-like protein to regulate anther dehiscence in rice . It was pointed out that a RING-type E3 ubiquitin ligase, POLLEN TUBE BLOCKED 1 (PTB1), positively regulated the rice panicle seed setting rate by promoting pollen tube growth . OsAP65, a rice aspartic protease, was essential for male fertility and played a important role in pollen germination and pollen tube growth . Chueasiri et al.  quantified gene expression in anthers of temperature-sensitive rice plants grown in controlled growth rooms (26 °C and 32 °C) for fertile and sterile conditions, the results indicated that plant orosomucoids-like proteins influenced sphingolipid homeostasis, and deletion of this gene affected spikelet fertility resulting from abnormal pollen development. The transcription factor bHLH142 was identified as a pivotal role in tapetal programmed cell death and pollen development during early meiosis in rice . In our study, the expression profile revealed that 15 spikelet genes annotated to GO database including 3 pollen development, 9 pollen exine formation, 1 pollen germination, 1 pollen tube development, and 1 sporopollenin biosynthetic process (pollen exine formation) were all down-regulated in ZNN-vs-ZNH (Additional file 15: Table S5). Many genes including 4 microsporogenesis, 11 flower development, 13 pollen development, 3 pollen tube growth, 12 pollen germination, 12 pollen exine formation, 9 pollen wall assembly, and 14 sporopollenin biosynthetic process from GO annotation were identified as spikelet genes in response to high temperature interacting with high nitrogen level in ZHN-vs-ZHH (Additional file 15: Table S5). Moreover, we found that 8 pollen exine formation, and 8 sporopollenin biosynthetic process DEGs with all genes down-regulated were co-expressed. Interestingly, in these co-expressed DEGs, the log2Ratio absolute value of spikelet genes including 1 pollen development gene (CL3271.Contig1), 7 pollen exine formation genes (Unigene45103, Unigene32014, Unigene32018, Unigene32017, Unigene42236, Unigene42231, Unigene42234), and 5 sporopollenin biosynthetic process genes (Unigene43852, Unigene32623, Unigene23940, Unigene23939, Unigene23941) of ZNN-vs-ZNH was lower than that of ZHN-vs-ZHH (Additional file 17: Table S7). The co-expressed DEGs especially pollen exine formation and sporopollenin biosynthetic process genes may suggest they play important roles in rice spikelet in response to high temperature at high nitrogen level. Accordingly, high temperature at meiosis stage significantly decreased spikelet fertility, and spikelet fertility decreased more significantly under high temperature interacting with high nitrogen level (Fig. 7). Therefore, these genes may be good candidates for crop improvement. In addition, we found 151 DEGs with 40 up-regulated and 111 down-regulated genes, and 323 DEGs with 150 up-regulated and 173 down-regulated genes in response to high temperature were identified in ZNN-vs-ZNH and ZHN-vs-ZHH, respectively (Additional file 15: Table S5), which indicating more DEGs were related to high temperature at high nitrogen level.
In summary, we performed a combination of RNA-seq and digital gene expression sequencing to identify the genes involved in rice spikelet treated by different temperatures and nitrogen levels. This study produced abundant data for research on the molecular mechanisms of the rice spikelet. We highlight the link between the pollen exine formation, sporopollenin biosynthetic process genes and the decreased spikelet fertility. To the best of our knowledge, the present study is the first to attempt to perform a de novo assembly of transcriptome sequencing of the temperature and nitrogen’s interactive effects in rice spikelet, which may extend our understanding of the complex molecular and cellular events in spikelet and facilitate identification of temperature and nitrogen’s interactive effects on plants.
Availability of supporting data
RNA-seq data used in the present study have been deposited into the NCBI Sequence Read Archive (SRA, http://www.ncbi.nlm.nih.gov/sra/) under the accession number of SRP058792 (SRR2043067, SRR2043068, SRR2043070, SRR2043072). All the supporting data are included as additional files.
We acknowledge the Beijing Genomics Institute (BGI) for its assistance in original data processing and related bioinformatics analysis. This work was supported by the “Special Scientific Research Foundation of Meteorological Public Welfare Industry of China (Grant No: GYHY201406025 and GYHY201006025)” and by the “Special Scientific Research Foundation of Agricultural Public Welfare Industry of China (Grant No: 201403002)” in Jiangxi Agricultural University.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- FAO. World Agriculture: Towards 2015/2030 Summary Report. Rome, Italy: FAO; 2002.Google Scholar
- Kim HY, Ko J, Kang S, Tenhunen J. Impacts of climate change on paddy rice yield in a temperate climate. Global Change Biol. 2013;19(2):548–62.View ArticleGoogle Scholar
- IPCC. Summary for policymakers. In: Metz B, Davidson OR, Bosch PR, Dave R, Meyer LA, editors. Climate Change 2007: Mitigation. Contribution of Working Group III to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge. UK and New York: Cambridge University Press; 2007. p. 1–24.Google Scholar
- Prasad PVV, Boote KJ, Allen Jr LH, Sheehy JE, Thomas JMG. Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress. Field Crop Res. 2006;95(2):398–411.View ArticleGoogle Scholar
- Jagadish SV, Muthurajan R, Oane R, Wheeler TR, Heuer S, Bennett J, et al. Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.). J Exp Bot. 2010;61(1):143–56.View ArticlePubMed CentralPubMedGoogle Scholar
- Peng S, Huang J, Sheehy JE, Laza RC, Visperas RM, Zhong X, et al. Rice yields decline with higher night temperature from global warming. Proc Natl Acad Sci U S A. 2004;101(27):9971–5.View ArticlePubMed CentralPubMedGoogle Scholar
- Wu L, Chen X, Cui Z, Zhang W, Zhang F. Establishing a regional nitrogen management approach to mitigate greenhouse gas emission intensity from intensive smallholder maize production. PLoS One. 2014;9(5):e98481.View ArticlePubMed CentralPubMedGoogle Scholar
- Weerakoon WMW, Maruyama A, Ohba K. Impact of humidity on temperature-induced grain sterility in rice (Oryza sativa L). J Agron Crop Sci. 2008;194:135–40.View ArticleGoogle Scholar
- Madan P, Jagadish SV, Craufurd PQ, Fitzgerald M, Lafarge T, Wheeler TR. Effect of elevated CO2 and high temperature on seed-set and grain quality of rice. J Exp Bot. 2012;63(10):3843–52.View ArticlePubMed CentralPubMedGoogle Scholar
- Matsui T, Omasa K. Rice (Oryza sativa L.) cultivars tolerant to high temperature at flowering: anther characteristics. Ann Bot-London. 2002;89(6):683–7.View ArticleGoogle Scholar
- Endo M, Tsuchiya T, Hamada K, Kawamura S, Yano K, Ohshima M, et al. High temperatures cause male sterility in rice plants with transcriptional alterations during pollen development. Plant Cell Physiol. 2009;50(11):1911–22.View ArticlePubMedGoogle Scholar
- Zhang X, Li J, Liu A, Zou J, Zhou X, Xiang J, et al. Expression profile in rice panicle: insights into heat response mechanism at reproductive stage. PLoS One. 2012;7(11):e49652.View ArticlePubMed CentralPubMedGoogle Scholar
- Kelliher T, Egger RL, Zhang H, Walbot V. Unresolved issues in pre-meiotic anther development. Front Plant Sci. 2014;5:347.View ArticlePubMed CentralPubMedGoogle Scholar
- Frink CR, Waggoner PE, Ausubel JH. Nitrogen fertilizer: retrospect and prospect. Proc Natl Acad Sci U S A. 1999;96(4):1175–80.View ArticlePubMed CentralPubMedGoogle Scholar
- Crawford NM. Nitrate: nutrient and signal for plant growth. Plant Cell. 1995;7(7):859–68.View ArticlePubMed CentralPubMedGoogle Scholar
- Stitt M. Nitrate regulation of metabolism and growth. Curr Opin Plant Biol. 1999;2(3):178–86.View ArticlePubMedGoogle Scholar
- Good AG, Shrawat AK, Muench DG. Can less yield more? Is reducing nutrient input into the environment compatible with maintaining crop production? Trends Plant Sci. 2004;9(12):597–605.View ArticlePubMedGoogle Scholar
- Socolow RH. Nitrogen management and the future of food: lessons from the management of energy and carbon. Proc Natl Acad Sci U S A. 1999;96(11):6001–8.View ArticlePubMed CentralPubMedGoogle Scholar
- Nosengo N. Fertilized to death. Nature. 2003;425(6961):894–5.View ArticlePubMedGoogle Scholar
- Giles J. Nitrogen study fertilizes fears of pollution. Nature. 2005;433(7028):791.View ArticlePubMedGoogle Scholar
- Bi YM, Wang RL, Zhu T, Rothstein SJ. Global transcription profiling reveals differential responses to chronic nitrogen stress and putative nitrogen regulatory components in Arabidopsis. BMC Genomics. 2007;8:281.View ArticlePubMed CentralPubMedGoogle Scholar
- Galvan A, Fernandez E. Eukaryotic nitrate and nitrite transporters. Cell Mol Life Sci. 2001;58(2):225–33.View ArticlePubMedGoogle Scholar
- Howitt SM, Udvardi MK. Structure, function and regulation of ammonium transporters in plants. Biochim Biophys Acta. 2000;1465(1–2):152–70.View ArticlePubMedGoogle Scholar
- Campbell WH. Nitrate reductase structure, function and regulation: bridging the gap between biochemistry and physiology. Annu Rev Plant Physiol Plant Mol Biol. 1999;50:277–303.View ArticlePubMedGoogle Scholar
- Peng M, Bi YM, Zhu T, Rothstein SJ. Genome-wide analysis of Arabidopsis responsive transcriptome to nitrogen limitation and its regulation by the ubiquitin ligase gene NLA. Plant Mol Biol. 2007;65(6):775–97.View ArticlePubMedGoogle Scholar
- Lian X, Wang S, Zhang J, Feng Q, Zhang L, Fan D, et al. Expression profiles of 10,422 genes at early stage of low nitrogen stress in rice assayed using a cDNA microarray. Plant Mol Biol. 2006;60(5):617–31.View ArticlePubMedGoogle Scholar
- Wang R, Guegler K, LaBrie ST, Crawford NM. Genomic analysis of a nutrient response in Arabidopsis reveals diverse expression patterns and novel metabolic and potential regulatory genes induced by nitrate. Plant Cell. 2000;12(8):1491–509.View ArticlePubMed CentralPubMedGoogle Scholar
- Weng JH, Chen CY. Differences between Indica and Japonica rice varieties in CO2 exchange rates in response to leaf nitrogen and temperature. Photosynth Res. 1987;14(2):171–8.View ArticlePubMedGoogle Scholar
- Liu ZL, Li YJ, Hou HY, Zhu XC, Rai V, He XY, et al. Differences in the arbuscular mycorrhizal fungi-improved rice resistance to low temperature at two N levels: aspects of N and C metabolism on the plant side. Plant Physiol Bioch. 2013;71:87–95.View ArticleGoogle Scholar
- Satake T, Lee SY, Koike S, Kariya K. Male sterility caused by cooling temperature at the young microspore stage in rice plants. XXVII. Effects of water temperature and nitrogen application before the critical stage on the sterility by cooling at the critical stage. Jpn J Crop Sci. 1987;56:404–10.View ArticleGoogle Scholar
- Hayashi T. Analysis of factors related to enhanced sterility due to coolness at the young microspore stage under high nitrogen supply in rice (Oryza sativa L.). Bull Natl Agric Res Cent Tohoku Reg. 2011;113:67–96.Google Scholar
- Haas BJ, Zody MC. Advancing RNA-Seq analysis. Nat Biotechnol. 2010;28(5):421–3.View ArticlePubMedGoogle Scholar
- Cloonan N, Forrest AR, Kolle G, Gardiner BB, Faulkner GJ, Brown MK, et al. Stem cell transcriptome profiling via massive-scale mRNA sequencing. Nat Methods. 2008;5(7):613–9.View ArticlePubMedGoogle Scholar
- Wang Z, Gerstein M, Snyder M. RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet. 2009;10(1):57–63.View ArticlePubMed CentralPubMedGoogle Scholar
- Chen S, Wang D, Xu C, Ji C, Zhang X, Zhao X, et al. Responses of super rice (Oryza sativa L.) to different planting methods for grain yield and nitrogen-use efficiency in the single cropping season. PLoS One. 2014;9(8):e104950.View ArticlePubMed CentralPubMedGoogle Scholar
- Shi W, Muthurajan R, Rahman H, Selvam J, Peng S, Zou Y, et al. Source-ink dynamics and proteomic reprogramming under elevated night temperature and their impact on rice yield and grain quality. New Phytol. 2013;197(3):825–37.View ArticlePubMedGoogle Scholar
- Mohammed AR, Tarpley L. High nighttime temperatures affect rice productivity through altered pollen germination and spikelet fertility. Agr Forest Meteorol. 2009;149:999–1008.View ArticleGoogle Scholar
- Tang X, Zhang ZY, Zhang WJ, Zhao XM, Li X, Zhang D, et al. Global gene profiling of laser-captured pollen mother cells indicates molecular pathways and gene subfamilies involved in rice meiosis. Plant Physiol. 2010;154(4):1855–70.View ArticlePubMed CentralPubMedGoogle Scholar
- Chen R, Zhao X, Shao Z, Wei Z, Wang Y, Zhu L, et al. Rice UDP-glucose pyrophosphorylase1 is essential for pollen callose deposition and its cosuppression results in a new type of thermosensitive genic male sterility. Plant Cell. 2007;19(3):847–61.View ArticlePubMed CentralPubMedGoogle Scholar
- Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011;29(7):644–52.View ArticlePubMed CentralPubMedGoogle Scholar
- Iseli C, Jongeneel CV, Bucher P. ESTScan: a program for detecting, evaluating, and reconstructing potential coding regions in EST sequences. Proc Int Conf Intell Syst Mol Biol. 1999;138–48.Google Scholar
- Li R, Yu C, Li Y, Lam TW, Yiu SM, Kristiansen K, et al. SOAP2: an improved ultrafast tool for short read alignment. Bioinformatics. 2009;25(15):1966–7.View ArticlePubMedGoogle Scholar
- de Hoon MJ, Imoto S, Nolan J, Miyano S. Open source clustering software. Bioinformatics. 2004;20(9):1453–4.View ArticlePubMedGoogle Scholar
- Saldanha AJ. Java Treeview--extensible visualization of microarray data. Bioinformatics. 2004;20(17):3246–8.View ArticlePubMedGoogle Scholar
- Conesa A, Gotz S, Garcia-Gomez JM, Terol J, Talon M, Robles M. Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics. 2005;21(18):3674–6.View ArticlePubMedGoogle Scholar
- Ye J, Fang L, Zheng H, Zhang Y, Chen J, Zhang Z et al. WEGO: a web tool for plotting GO annotations. Nucleic Acids Res. 2006;34(Web Server issue):W293-7.Google Scholar
- Kanehisa M, Goto S. KEGG: kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 2000;28(1):27–30.View ArticlePubMed CentralPubMedGoogle Scholar
- Kanehisa M, Araki M, Goto S, Hattori M, Hirakawa M, Itoh M, et al. KEGG for linking genomes to life and the environment. Nucleic Acids Res. 2008;36(Database issue):D480–4.PubMed CentralPubMedGoogle Scholar
- Tatusov RL, Galperin MY, Natale DA, Koonin EV. The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res. 2000;28(1):33–6.View ArticlePubMed CentralPubMedGoogle Scholar
- Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008;5(7):621–8.View ArticlePubMedGoogle Scholar
- Audic S, Claverie JM. The significance of digital gene expression profiles. Genome Res. 1997;7(10):986–95.PubMedGoogle Scholar
- Benjamini Y, Drai D, Elmer G, Kafkafi N, Golani I. Controlling the false discovery rate in behavior genetics research. Behav Brain Res. 2001;125(1–2):279–84.View ArticlePubMedGoogle Scholar
- Lin MY, Chai KH, Ko SS, Kuang LY, Lur HS, Charng YY. A positive feedback loop between heat shock PROTEIN101 and heat stress-associated 32-KD protein modulates long-term acquired thermotolerance illustrating diverse heat stress responses in rice varieties. Plant Physiol. 2014;164(4):2045–53.View ArticlePubMed CentralPubMedGoogle Scholar
- Jung KH, Gho HJ, Nguyen MX, Kim SR, An G. Genome-wide expression analysis of HSP70 family genes in rice and identification of a cytosolic HSP70 gene highly induced under heat stress. Funct Integr Genomics. 2013;13(3):391–402.View ArticlePubMedGoogle Scholar
- Guan JC, Yeh CH, Lin YP, Ke YT, Chen MT, You JW, et al. A 9 bp cis-element in the promoters of class I small heat shock protein genes on chromosome 3 in rice mediates L-azetidine-2-carboxylic acid and heat shock responses. J Exp Bot. 2010;61(15):4249–61.View ArticlePubMed CentralPubMedGoogle Scholar
- Sarkar NK, Kim YK, Grover A. Rice sHsp genes: genomic organization and expression profiling under stress and development. BMC Genomics. 2009;10:393.View ArticlePubMed CentralPubMedGoogle Scholar
- Waters ER. The evolution, function, structure, and expression of the plant sHSPs. J Exp Bot. 2013;64(2):391–403.View ArticlePubMedGoogle Scholar
- Scafaro AP, Haynes PA, Atwell BJ. Physiological and molecular changes in Oryza meridionalis Ng., a heat-tolerant species of wild rice. J Exp Bot. 2010;61(1):191–202.View ArticlePubMed CentralPubMedGoogle Scholar
- Sung DY, Vierling E, Guy CL. Comprehensive expression profile analysis of the Arabidopsis Hsp70 gene family. Plant Physiol. 2001;126(2):789–800.View ArticlePubMed CentralPubMedGoogle Scholar
- Agarwal M, Sahi C, Katiyar-Agarwal S, Agarwal S, Young T, Gallie DR, et al. Molecular characterization of rice hsp101: complementation of yeast hsp104 mutation by disaggregation of protein granules and differential expression in indica and japonica rice types. Plant Mol Biol. 2003;51(4):543–53.View ArticlePubMedGoogle Scholar
- Qi Y, Wang H, Zou Y, Liu C, Liu Y, Wang Y, et al. Over-expression of mitochondrial heat shock protein 70 suppresses programmed cell death in rice. FEBS Lett. 2011;585(1):231–9.View ArticlePubMedGoogle Scholar
- Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U. AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV. Plant J. 2011;66(6):983–95.View ArticlePubMedGoogle Scholar
- Yamanouchi U, Yano M, Lin H, Ashikari M, Yamada K. A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein. Proc Natl Acad Sci U S A. 2002;99(11):7530–5.View ArticlePubMed CentralPubMedGoogle Scholar
- El-Kereamy A, Bi YM, Ranathunge K, Beatty PH, Good AG, Rothstein SJ. The rice R2R3-MYB transcription factor OsMYB55 is involved in the tolerance to high temperature and modulates amino acid metabolism. PLoS One. 2012;7(12), e52030.View ArticlePubMed CentralPubMedGoogle Scholar
- Castaings L, Marchive C, Meyer C, Krapp A. Nitrogen signalling in Arabidopsis: how to obtain insights into a complex signalling network. J Exp Bot. 2011;62(4):1391–7.View ArticlePubMedGoogle Scholar
- Hirano K, Aya K, Hobo T, Sakakibara H, Kojima M, Shim RA, et al. Comprehensive transcriptome analysis of phytohormone biosynthesis and signaling genes in microspore/pollen and tapetum of rice. Plant Cell Physiol. 2008;49(10):1429–50.View ArticlePubMed CentralPubMedGoogle Scholar
- Chhun T, Aya K, Asano K, Yamamoto E, Morinaka Y, Watanabe M, et al. Gibberellin regulates pollen viability and pollen tube growth in rice. Plant Cell. 2007;19(12):3876–88.View ArticlePubMed CentralPubMedGoogle Scholar
- Sakata T, Oda S, Tsunaga Y, Shomura H, Kawagishi-Kobayashi M, Aya K, et al. Reduction of gibberellin by low temperature disrupts pollen development in rice. Plant Physiol. 2014;164(4):2011–9.View ArticlePubMed CentralPubMedGoogle Scholar
- Liao C, Peng Y, Ma W, Liu R, Li C, Li X. Proteomic analysis revealed nitrogen-mediated metabolic, developmental, and hormonal regulation of maize (Zea mays L.) ear growth. J Exp Bot. 2012;63(14):5275–88.View ArticlePubMed CentralPubMedGoogle Scholar
- Harke MJ, Gobler CJ. Global transcriptional responses of the toxic cyanobacterium, Microcystis aeruginosa, to nitrogen stress, phosphorus stress, and growth on organic matter. PLoS One. 2013;8(7):e69834.View ArticlePubMed CentralPubMedGoogle Scholar
- Wang R, Okamoto M, Xing X, Crawford NM. Microarray analysis of the nitrate response in Arabidopsis roots and shoots reveals over 1,000 rapidly responding genes and new linkages to glucose, trehalose-6-phosphate, iron, and sulfate metabolism. Plant Physiol. 2003;132(2):556–67.View ArticlePubMed CentralPubMedGoogle Scholar
- Agarwal P, Reddy MP, Chikara J. WRKY: its structure, evolutionary relationship, DNA-binding selectivity, role in stress tolerance and development of plants. Mol Biol Rep. 2011;38(6):3883–96.View ArticlePubMedGoogle Scholar
- Chen H, Lai Z, Shi J, Xiao Y, Chen Z, Xu X. Roles of arabidopsis WRKY18, WRKY40 and WRKY60 transcription factors in plant responses to abscisic acid and abiotic stress. BMC Plant Biol. 2010;10:281.View ArticlePubMed CentralPubMedGoogle Scholar
- Higashitani A. High temperature injury and auxin biosynthesis in microsporogenesis. Front Plant Sci. 2013;4:47.View ArticlePubMed CentralPubMedGoogle Scholar
- Wu L, Guan Y, Wu Z, Yang K, Lv J, Converse R, et al. OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice. Plant Cell Rep. 2014;33(11):1881–99.View ArticlePubMed CentralPubMedGoogle Scholar
- Ling S, Chen C, Wang Y, Sun X, Lu Z, Ouyang Y, et al. The mature anther-preferentially expressed genes are associated with pollen fertility, pollen germination and anther dehiscence in rice. BMC Genomics. 2015;16:101.View ArticlePubMed CentralPubMedGoogle Scholar
- Zhou S, Wang Y, Li W, Zhao Z, Ren Y, Wang Y, et al. Pollen semi-sterility1 encodes a kinesin-1-like protein important for male meiosis, anther dehiscence, and fertility in rice. Plant Cell. 2011;23(1):111–29.View ArticlePubMed CentralPubMedGoogle Scholar
- Li S, Li W, Huang B, Cao X, Zhou X, Ye S, et al. Natural variation in PTB1 regulates rice seed setting rate by controlling pollen tube growth. Nat Commun. 2013;4:2793.PubMedGoogle Scholar
- Huang J, Zhao X, Cheng K, Jiang Y, Ouyang Y, Xu C, et al. OsAP65, a rice aspartic protease, is essential for male fertility and plays a role in pollen germination and pollen tube growth. J Exp Bot. 2013;64(11):3351–60.View ArticlePubMed CentralPubMedGoogle Scholar
- Chueasiri C, Chunthong K, Pitnjam K, Chakhonkaen S, Sangarwut N, Sangsawang K, et al. Rice ORMDL controls sphingolipid homeostasis affecting fertility resulting from abnormal pollen development. PLoS One. 2014;9(9):e106386.View ArticlePubMed CentralPubMedGoogle Scholar
- Ko SS, Li MJ, Sun-Ben Ku M, Ho YC, Lin YJ, Chuang MH, et al. The bHLH142 transcription factor coordinates with TDR1 to modulate the expression of EAT1 and regulate pollen development in rice. Plant Cell. 2014;26(6):2486–504.View ArticlePubMed CentralPubMedGoogle Scholar