- Open Access
Genomics research at Bioinformatics of Genome Regulation and Structure\ Systems Biology (BGRS\SB) conferences in Novosibirsk
BMC Genomics volume 20, Article number: 322 (2019)
In this Special Issue of BMC Genomics we present the studies on computational genomics discussed at BGRS\SB-2018 (Bioinformatics of Genome Regulation and Structure\Systems Biology) multi-conference (http://conf.bionet.nsc.ru/sbb2018/en/). This traditional biannual meeting on bioinformatics and systems biology in Novosibirsk Academgorodok has longest history since 1998 and wide range of special post-conference journal issues covering modern computational genomics and systems biology applications. Parallel special issues of BioMed Central journals after the BGRS conference series include manuscripts in the fields of plant biology, genomics, bioinformatics and genetics, and collated as BMC Systems Biology, BMC Evolutionary Biology, BMC Bioinformatics, BMC Genetics, BMC Medical Genomics and BMC Plant Biology Supplements this year [1,2,3], as well as in the past [4,5,6,7]. First special issue after BGRS\SB-2014 conference at BMC Genomics was published in 2014 (https://bmcgenomics.biomedcentral.com/articles/supplements/volume-15-supplement-12), and continued by Supplement issue in 2016 .
Note the original research paper on oscillatory dynamics in the synthesis of viral components by V.A.Likhoshvai  that served as excellent example of science background for next publications in the gene networks theory by the authors group. We regret to inform that outstanding scientist Dr. Vitaly A. Likhoshvai passed away February this year. His ideas on mathematical models were presented later at BMC Microbiology special issue associated with bioinformatics event - Young Scientists School “Systems Biology and Bioinformatics” (SBB’2015) , and evolutionary model published recently at Sci Rep journal , continued by Dr. Tamara M. Khlebodarova and colleagues.
Do discuss genomics researches we would also like to bring attention of the reader to the highlights of genomics and bioinformatics research at “Belyaev Readings-2017” - academician D.K. Belyaev memorial conference on genetics (http://conf.bionet.nsc.ru/belyaev100/en), which was held in Novosibirsk in 2017, in BioMed Central journal series [11,12,13,14,15,16] and Vavilov journal of genetics and breeding [17, 18].
The papers here were presented in the frames of BGRS\SB–affiliated symposium “Systems Computational Biology” (http://conf.bionet.nsc.ru/bgrssb2018/en/) and at Tenth International Young Scientists School SBB-2018 (http://conf.bionet.nsc.ru/sbb2018/en/), educational event on systems biology with own history and publication record [9, 19, 20], see also BMC Genomics Supplement (https://bmcgenomics.biomedcentral.com/articles/supplements/volume-16-supplement-13).
Though genomics field is quite large, here we collected works from general computational modeling, computational approaches for human genomics and databases to applications on animal models and plant genomics. Below is a summary of the papers with some references to background publications.
Wang and coauthors  present method for chromosome structure analysis on cooperation of transcription factors (TF) based on integration of DNA sequences, ChIP-Seq and ChIA-PET data. ChIA-PET technology was initially designed for transcription factor binding studies ; it continues extends application fields including theoretical bioinformatics models [23, 24]. Chromosomal architecture has hierarchical structure from large chromosome territories to A/B compartments, topologically associated domains to chromatin loops. Chromatin loops, plays an important role in cellular functions, can bring distal regulatory elements, such as enhancers in linear DNA, to the promoters of target genes in 3D space. The authors proposed the HidPET (Hierarchical and Dynamics Analysis of TF Cooperation with ChIA-PET and ChIP-Seq Data) method for investigating the cooperation of transcription factors by the integrative analysis of available sequencing data. Based on four cancer cell datasets, the authors show association of 3D chromatin organization and gene with the hierarchical and dynamic prosperities of TFs binding.
Viktor Shamanskiy and co-authors  present software for mitochondrial imperfect interspersed repeats annotation. The algorithm developed is adapted to specific characteristics of mtDNA such as circularity and an excess of short repeats. The authors constructed interactive web available database ImtRDB depositing perfect and imperfect repeats positions in mtDNAs of more than 3500 Vertebrate species. The analyses of the collected data demonstrated that mtDNA imperfect repeats are usually short, associated with unfolded DNA structures and abundance of repeats is negatively associated with GC content. Somatic perturbations of mtDNA are accumulating with age, thus it is of great importance to uncover the main sources of mtDNA instability. Such instability (somatic mtDNA deletions) depends on imperfect repeats between distant mtDNA segments. The ImtRDB database (http://bioinfodbs.kantiana.ru/ImtRDB/) presents novel type of data keeping all the types of imperfect repeats in circular mtDNA. The work continues studies on tandem repeats and sequencing quality control published at BMC Genomics [26, 27].
Andrey Yurchenko et al.  present application in animal genomics - high-density genotyping on sheep breeds. The genomes of local sheep breeds are valuable sources of genomic variants for understanding mechanisms of response to adaptation and artificial selection. The authors performed a high-density genotyping and comprehensive scans for signatures of selection in the genomes from 15 local sheep breeds reared across Russia. The results demonstrated that the genomes of Russian sheep breeds contain multiple regions under putative selection containing known candidate genes related to morphology, adaptation, and domestication (e.g., KITLG, KIT, MITF, MC1R), wool quality and, growth and feed intake, reproduction and milk-related traits. This work represents the first comprehensive scan for signatures of selection in genomes of local sheep breeds from the Russia of both European and Asian origins. These gene data could be the focus of future efforts on the improvement of local and multinational breeds using the rapidly-developing plethora of contemporary genomics tools.
Daria Konina et al.  analyzed long noncoding RNA - LINC01420. The major part of human genome is transcribed and produces a large number of long noncoding RNAs (lncRNAs). LncRNAs are transcripts with the length more than 200 nucleotides that do not possess long open reading frames. Nowadays there are many evidences that lncRNAs play important role in the regulation of gene expression, are involved in the development of various human diseases. The function of the major part of annotated transcripts is currently unknown, whereas different lncRNAs annotations tend to have low overlap. Recent studies revealed that some lncRNAs have small open reading frames (smORFs) that produce the functional microproteins. However, the question whether the function of such genes is determined by microprotein or RNA itself or both remains open. Konina and colleagues investigated widely expressed lncRNA LINC01420, the function of which was not described at the time of the beginning of the study. The authors used reverse transcription PCR and rapid amplification of cDNA ends (RACE) analysis to determine the structure of the LINC01420 transcript. LINC01420 has two isoforms that differ in length of the last exon and are localized predominantly in the cytoplasm. D’Lima et al.  found smORF in the first exon of the LINC01420 gene. This smORF produces functional microprotein named non-annotated P-body dissociating polypeptide (NoBody). However, Konina and colleagues  provide here new facts about LINC01420 transcript and its function.
Larisa Fedoseeva et al.  discuss the differences in brain stem transcriptional profiling in rat models. Essential (primary) hypertension is a widely spread disease with underlying genetic predispositions, many of which still remain unknown . The development of essential hypertension is associated with a wide range of mechanisms. The brain stem neurons are essential for the homeostatic regulation of arterial pressure as they control baroreflex and sympathetic nerve activity. The ISIAH (Inherited Stress Induced Arterial Hypertension) rats reproduce the human stress-sensitive hypertensive disease with predominant activation of the neuroendocrine hypothalamic-pituitary-adrenal and sympathetic adrenal axes. RNA-Seq analysis of the brain stems from the hypertensive ISIAH and normotensive control WAG (Wistar Albino Glaxo) rats was performed to identify the differentially expressed genes and the main central mechanisms (biological processes and metabolic pathways) contributing to the hypertensive state in the ISIAH rats. The authors revealed set of differentially expressed genes and discussed their association with hypertension and blood pressure regulation, as well as association with central nervous system diseases. This study also continues research on transcriptome profiling on ISIAH rats published at BGRS special issues in 2016 [33, 34].
Nikita Ivanisenko et al.  discuss the role of c-FLIP/NEMO interaction in the CD95 network via rational design of molecular probes. The authors analyze the molecular interactions orchestrating death receptor signaling networks. CD95/Fas is a member of the DR family, which is a subfamily of the tumor necrosis factor receptor superfamily. Activation of CD95 initiates the extrinsic apoptosis pathway. The CD95-induced apoptotic signal is mediated via the formation of a death-inducing signaling complex (DISC), which comprises CD95, FADD, procaspases-8, − 10 and c-FLIPs (cellular FLICE-like inhibitory proteins). The DISC serves as a central platform for procaspase-8 activation, which subsequently initiates an apoptotic cascade. Recently, it has been demonstrated that, at the DISC, procaspase-8 proteins form so-called DED (Death Effector Domain) chains or filaments via interactions of their DED motifs. NF-κB activation is mediated via activation of the IKK complex containing the regulatory subunit NEMO (nuclear factor (NF)-κB essential modulator). c-FLIP proteins have been reported to play an important role in NF-κB induction and, in particular, in CD95-mediated NF-κB induction. The authors created the homology model of the c-FLIP/NEMO complex using the reported structure of the v-FLIP/NEMO complex, and rationally designed peptides targeting this complex. Further insight into the function of c-FLIP/NEMO complex was provided by the analysis of evolutionary conservation of interacting regions which demonstrated that this interaction is common in distinct mammalian species. These findings provide new opportunities for the design of new specific anti-cancer therapies based on the inhibition of death receptor-mediated anti-apoptosis pathways. Protein modeling research of the same group on SOD1 was published last year at BMC Structural Biology as selected article from Belyaev Conference 2017 .
The next group of papers in dedicated to plant genetics. Rozanova et al.  focused on identification of SNPs associated with barley resistance to novel isolates of Pyrenophora teres F. teres. Net blotch caused by Pyrenophora/Drechslera teres is a major foliar disease of barley, resulting in 40% yield losses of susceptible cultivars. Rozanova et al. detected seven genomic regions in barley associated with the resistance to four novel Pyrenophora teres F. teres isolates, including one novel isolate-specific locus. The significant SNPs detected in this study can be converted to convenient PCR-markers for accelerated breeding of resistant barley cultivars. Note the research of the authors group on SNP analysis in barley on microchips .
Konstantin Kozlov et al.  present regression models for plant genomics - flowering time estimation in wild chickpea. This work is published as BGRS\SB-2018 Supplement to BMC Plant Biology issue. Accurate prediction of crop flowering time is required for reaching maximal farm efficiency. Several models developed to accomplish this goal are based on deep knowledge of plant phenology, requiring large investment for every individual crop or new variety. Cultivars of chickpea, Cicer arietanum, are currently being improved by introgressing wild C. reticulatum biodiversity with very different flowering time requirements. The authors built a novel model for flowering time of wild chickpeas collected at 21 different sites in Turkey and grown in 4 distinct environmental conditions over several different years and seasons. Kozlov and co-authors propose a general approach, in which the analytic forms of dependence of flowering time on climatic parameters, their regression coefficients, and a set of predictors are inferred automatically by stochastic minimization of the deviation of the model output from data. They also identified several genomic markers in chickpea associated with different reactions to climatic factor changes.
We invite our readers worldwide to attend our next events - Systems Biology and Bioinformatics Young Scientists School SBB-2019 which will be held in Novosibirsk, and Vavilov Society Congress - 2019 in St. Petersburg, Russia, in summer 2019. Genomics field will be highlighted at VII Congress of Vavilov Society of Geneticists and Breeders organized by of Saint Petersburg State University 18–22 June 2019 (https://events.spbu.ru/events/genetic-selection-2019). Soon after, 24–29 June 2019, 5th International scientific conference “Plant genetics, genomics, bioinformatics and biotechnology” (PlantGen2019) will be organized in Novosibirsk, Russia (http://conf.bionet.nsc.ru/plantgen2019/en/) focusing on plant genomics field. Next BGRS\SB-2020 event is scheduled to June 2020 in Novosibirsk, Russia.
Orlov YL, Salina EA, Eslami G, Kochetov AV. Plant biology research at BGRS-2018. BMC Plant Biol. 2019;19(Suppl 1):56.
Tatarinova TV, Chen M, Orlov YL. Bioinformatics research at BGRS-2018. BMC Bioinformatics. 2019;20(Suppl 1):33.
Orlov YL, Baranova AV, Kolchanov NA, Moroz LL. Evolutionary biology and biodiversity research at BGRS-2018. BMC Evolutionary Biology. 2019;19(Suppl 1):43.
Orlov YL, Baranova AV, Hofestadt R, Kolchanov NA. Computational genomics at BGRS\SB-2016: introductory note. BMC Genomics. 2016;17(Suppl 14):996.
Orlov YL, Baranova AV, Salina EA. Computational plant bioscience at BGRS\SB-2016. introductory note. BMC Plant Biology 2016;16(Suppl 3):243.
Orlov YL, Baranova AV, Markel AL. Computational models in genetics at BGRS\SB-2016: introductory note. BMC Genet. 2016;17(Suppl 3):155.
Baranova AV, Orlov YL. Evolutionary biology at BGRS\SB-2016. BMC Evol Biol. 2017;17(Suppl 1):21.
Likhoshvai VA, Khlebodarova TM, Bazhan SI, Gainova IA, Chereshnev VA, Bocharov GA. Mathematical model of the tat-rev regulation of HIV-1 replication in an activated cell predicts the existence of oscillatory dynamics in the synthesis of viral components. BMC Genomics. 2014;15(Suppl 12):S1.
Khlebodarova TM, Ree NA, Likhoshvai VA. On the control mechanisms of the nitrite level in Escherichia coli cells: the mathematical model. BMC Microbiol. 2016;16(Suppl 1):7.
Khlebodarova TM, Likhoshvai VA. Persister cells - a plausible outcome of neutral Coevolutionary drift. Sci Rep. 2018;8(1):14309.
Orlov YL, Baranova AV, Tatarinova TV, Kolchanov NA. Genetics at Belyaev conference - 2017: introductory note. BMC Genet. 2017;18(Suppl 1):116.
Orlov YL, Baranova AV, Chen M, Salina EA. Plant biology at Belyaev conference - 2017. BMC Plant Biol. 2017;17(Suppl 2):257.
Orlov YL, Baranova AV, Hofestädt R, Kolchanov NA. Genomics at Belyaev conference - 2017. BMC Genomics. 2018;19(Suppl 3):79.
Orlov YL, Baranova AV, Herbeck YE. Evolutionary biology at Belyaev conference - 2017. BMC Evol Biol. 2017;17(Suppl 2):260.
Orlov YL, Moroz LL, Baranova AV. Neuroscience researches at Belyaev conference-2017. BMC Neurosci. 2018;19(Suppl 1):14.
Orlov YL, Fernandez-Masso JR, Chen M, Baranova AV. Medical genomics at Belyaev conference - 2017. BMC Med Genet. 2018;11(Suppl 1):11.
Shumny VK. To the centenary of the birth of outstanding evolutionist Dmitri Konstantinovich Belyaev. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov. J Genet Breed. 2017;21(4):387–91. doi:10.18699/VJ17.256 (In Russian).
Trut LN, Kharlamova AV, Vladimirova AV, Herbeck YE. On selection of foxes for enhanced aggressiveness and its correlated implications. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov. J Genet Breed. 2017;21(4):392–401. doi:10.18699/VJ17.257 (In Russian).
Baranova AV, Orlov YL. The papers presented at 7th young scientists school "systems biology and bioinformatics" (SBB'15): introductory note. BMC Genet. 2016;17(Suppl 1):20.
Orlov YL, Tatarinova TV, Zakhartsev MV, Kolchanov NA. Introduction to the 9th young scientists school on systems biology and bioinformatics (SBB'2017). J Bioinform Comput Biol. 2018;16(1):1802001.
Wang R, Wang Y, Zhang X, Zhang Y, Du X, Fang Y, Li G. Hierarchical cooperation of transcription factors from integration analysis of DNA sequences and ChIP-Seq and ChIA-PET data. BMC Genomics 2019;20(Suppl 3). https://doi.org/10.1186/s12864-019-5535-2.
Fullwood MJ, Liu MH, Pan YF, Liu J, Xu H, Mohamed YB, et al. An oestrogen-receptor-alpha-bound human chromatin interactome. Nature. 2009;462(7269):58–64.
Li G, Cai L, Chang H, Hong P, Zhou Q, Kulakova EV, et al. Chromatin interaction analysis with paired-end tag (ChIA-PET) sequencing technology and application. BMC Genomics. 2014;15(Suppl 12):S11.
Orlov YL, Thierry O, Bogomolov AG, Tsukanov AV, Kulakova EV, Galieva ER, et al. [Computer methods of analysis of chromosome contacts in the cell nucleus based on sequencing technology data]. Biomed Khim. 2017;63(5):418–422. doi: https://doi.org/10.18097/PBMC20176305418. Russian.
Shamanskiy VN, Timonina VN, Popadin KY, Gunbin KV. ImtRDB: a database and software for mitochondrial imperfect interspersed repeats annotation. BMC Genomics. 2019;20(Suppl 3). https://doi.org/10.1186/s12864-019-5536-1.
Evtushenko EV, Levitsky VG, Elisafenko EA, Gunbin KV, Belousov AI, Šafář J, et al. The expansion of heterochromatin blocks in rye reflects the co-amplification of tandem repeats and adjacent transposable elements. BMC Genomics. 2016;17:337.
Naumenko FM, Abnizova II, Beka N, Genaev MA, Orlov YL. Novel read density distribution score shows possible aligner artefacts, when mapping a single chromosome. BMC Genomics. 2018;19(Suppl 3):92.
Yurchenko AA, Deniskova TE, Yudin NS, Dotsev AV, Khamiruev TN, Selionova MI, Egorov SV, Reyer H, Wimmers K, Brem G, Zinovieva NA, Larkin DM. High-density genotyping reveals signatures of selection related to acclimation and economically important traits in 15 local sheep breeds from Russia. BMC Genomics. 2019;20(Suppl 3). https://doi.org/10.1186/s12864-019-5537-0.
Konina DO, Filatova AY, Skoblov MY. LINC01420 RNA structure and influence on cell physiology. BMC Genomics. 2019;20(Suppl 3). https://doi.org/10.1186/s12864-019-5538-z.
D'Lima NG, Ma J, Winkler L, Chu Q, Loh KH, Corpuz EO, et al. A human microprotein that interacts with the mRNA decapping complex. Nat Chem Biol. 2016;13(2):174–80.
Fedoseeva LA, Klimov LO, Ershov NI, Efimov VM. Markel AL. Orlov YL: Redina OE. The differences in brain stem transcriptional profiling in hypertensive ISIAH and normotensive WAG rats. BMC Genomics. 2019;20(Suppl 3). https://doi.org/10.1186/s12864-019-5540-5.
Markel AL, Redina OE. [Epigenetic Mechanisms of Blood-Pressure Regulation].Mol Biol (Mosk). 2018;52(2):172–189. doi: 10.7868/S0026898418020027. (In Russian).
Fedoseeva LA, Klimov LO, Ershov NI, Alexandrovich YV, Efimov VM, Markel AL, Redina OE. Molecular determinants of the adrenal gland functioning related to stress-sensitive hypertension in ISIAH rats. BMC Genomics. 2016;17(Suppl 14):989.
Fedoseeva LA, Ryazanova MA, Ershov NI, Markel AL, Redina OE. Comparative transcriptional profiling of renal cortex in rats with inherited stress-induced arterial hypertension and normotensive Wistar albino Glaxo rats. BMC Genet. 2016;17(Suppl 1):12.
Ivanisenko N, Buchbinder JH, Espe J, Richter M, Bollmann M, Hillert L, Ivanisenko V, Lavrik IN. Delineating the role of c-FLIP/NEMO interaction in the CD95 network via rational design of molecular probes. BMC Genomics. 2019;20(Suppl 3). https://doi.org/10.1186/s12864-019-5539-y.
Alemasov NA, Ivanisenko NV, Ramachandran S, Ivanisenko VA. Molecular mechanisms underlying the impact of mutations in SOD1 on its conformational properties associated with amyotrophic lateral sclerosis as revealed with molecular modelling. BMC Struct Biol. 2018;18(Suppl 1):1.
Rozanova IV, Lashina NM, Mustafin ZS, Gorobets SA. Efimov VM. Afanasenko OS: Khlestkina EK. SNPs associated with barley resistance to novel isolates of Pyrenophora teres F. teres. BMC Genomics. 2019;20(Suppl 3). https://doi.org/10.1186/s12864-019-5623-3.
Bykova IV, Lashina NM, Efimov VM, Afanasenko OS, Khlestkina EK. Identification of 50 K Illumina-chip SNPs associated with resistance to spot blotch in barley. BMC Plant Biol. 2017;17(Suppl 2):250.
Kozlov K, Singh A, Berger J, Bishop-von Wettberg E, Kahraman A, Aydogan A, Cook D, Nuzhdin S, Samsonova M. Non-linear regression models for time to flowering in wild chickpea combine genetic and climatic factors. BMC Plant Biol. 2019;19(Suppl 2).
We are grateful to Professors A.V. Baranova, M. Chen, P. Shanmughavel, R. Hofestädt, H.V. Westerhoff and M. Barberis, for organization of the multi-conference and providing platform for international bioinformatics research, and to Institute of Cytology and Genetics SB RAS (budget project 0324-2019-0040) and Novosibirsk State University for hosting the conference and logistics support. The BGRS\SB-2018 conference organization and computer genomics session were supported by the Russian Foundation of Basic Research, Russian-German and Sino-Russian Virtual Bioinformatics Network. Organization of the symposium “Biodiversity: genomics and evolution” presenting computational genomics problems was supported by the Ministry of Education and Science of the Russian Federation grant #14.W03.31.0015.
The guest editors of the special issue are grateful to the conference committee members and reviewers who helped in the articles editing and issue preparation: Xiaoyong Zou (School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, China), Vladimir Babenko, Ulyana Zubairova, Nariman Battulin and Veniamin Fishman (Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia), Lyudmila F. Gulyaeva (The Institute of Molecular Biology and Biophysics, Novosibirsk, Russia), Alexander M. Ishov (University of Florida College of Medicine, Gainesville, FL, USA), Oleg Shuvalov (Institute of Cytology of the RAS, Saint-Petersburg, Russia), Dmitry S. Karpov (Institute of Biomedical Chemistry, Moscow, Russia), Filipe Pereira (Interdisciplinary Centre of Marine and Environmental Research CIIMAR, Portugal), Sergey V. Petoukhov (Mechanical Engineering Research Institute RAS, Moscow, Russia), Ajey Kumar Pathak (Indian Council of Agricultural Research, Lucknow, Uttar Pradesh, India), Zhao (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China), Svetlana Limborskaia (Institute of Molecular Genetics RAS, Moscow), Huiguang Wu (National University of Inner Mongolia, China), Andre Marakhonov (Research Centre for Medical Genetics, Moscow, Russia), Alena Zolotarenko (Vavilov Institute of General Genetics RAS, Moscow, Russia), Daniel Ruzek (Institute of Parasitology, Biology Centre of the Czech Academy), Dmitri Karpov (IMB RAS, Moscow, Russia), Luwanika Mlera (National Institutes of Health, USA), Anastasia Bakulina (Novosibirsk State University, Novosibirsk, Russia), Srinivasan Ramachandran (Institute Integrative Biology, New Delphi, India), Melanie Correll (University of Florida, Gainesville, FL, USA), Tamara Amstislavskaya (NII Fundamental Physiology and Medicine RAS, Russia), Igor Ponomarev (University of Texas at Austin, USA), Morgane Boone (University of California, San Francisco, USA), Tatiana Tatarinova (University of La Verne, CA, USA), Mirjana Stajić (Belgrade University, Serbia), Robert Brueggeman (North Dakota State University, USA) and Yerlan Turuspekov (Institute of Plant Biology and Biotechnology, Almaty, Kazakhstan).
This article did not receive sponsorship for publication.
About this supplement
This article has been published as part of BMC Genomics Volume 20 Supplement 3, 2019: Selected articles from BGRS\SB-2018: genomics. The full contents of the supplement are available online at https://bmcgenomics.biomedcentral.com/articles/supplements/volume-20-supplement-3.
Ethics approval and consent to participate
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Orlov, Y.L., Kochetov, A.V., Li, G. et al. Genomics research at Bioinformatics of Genome Regulation and Structure\ Systems Biology (BGRS\SB) conferences in Novosibirsk. BMC Genomics 20, 322 (2019). https://doi.org/10.1186/s12864-019-5707-0