- Research article
- Open Access
The genome-wide binding profile of the Sulfolobus solfataricustranscription factor Ss-LrpB shows binding events beyond direct transcription regulation
© Nguyen-Duc et al.; licensee BioMed Central Ltd. 2013
- Received: 13 June 2013
- Accepted: 15 November 2013
- Published: 25 November 2013
Gene regulatory processes are largely resulting from binding of transcription factors to specific genomic targets. Leucine-responsive Regulatory Protein (Lrp) is a prevalent transcription factor family in prokaryotes, however, little information is available on biological functions of these proteins in archaea. Here, we study genome-wide binding of the Lrp-like transcription factor Ss-LrpB from Sulfolobus solfataricus.
Chromatin immunoprecipitation in combination with DNA microarray analysis (ChIP-chip) has revealed that Ss-LrpB interacts with 36 additional loci besides the four previously identified local targets. Only a subset of the newly identified binding targets, concentrated in a highly variable IS-dense genomic region, is also bound in vitro by pure Ss-LrpB. There is no clear relationship between the in vitro measured DNA-binding specificity of Ss-LrpB and the in vivo association suggesting a limited permissivity of the crenarchaeal chromatin for transcription factor binding. Of 37 identified binding regions, 29 are co-bound by LysM, another Lrp-like transcription factor in S. solfataricus. Comparative gene expression analysis in an Ss-lrpB mutant strain shows no significant Ss-LrpB-mediated regulation for most targeted genes, with exception of the CRISPR B cluster, which is activated by Ss-LrpB through binding to a specific motif in the leader region.
The genome-wide binding profile presented here implies that Ss-LrpB is associated at additional genomic binding sites besides the local gene targets, but acts as a specific transcription regulator in the tested growth conditions. Moreover, we have provided evidence that two Lrp-like transcription factors in S. solfataricus, Ss-LrpB and LysM, interact in vivo.
- Leucine-responsive regulatory protein
Transcription factors (TFs) belonging to the Leucine-responsive Regulatory Protein (Lrp) family (also known as AsnC or FFRP) are abundant in both bacteria and archaea [1–4]. A sequence analysis of 52 archaeal genomes indicated that they are all predicted to contain at least one lrp-like gene, lrp-like genes constituting in total about 8% of all non-general TF genes in archaea .
Whereas bacterial Lrp-like TFs regulate amino acid biosynthesis in response to nutritional availability , archaeal Lrp members also regulate genes belonging to energy, central metabolism and transport pathways [6–9]. Furthermore, it has been observed and/or predicted by sequence analyses that a subset of archaeal Lrp-like TFs do not interact with amino acids, in contrast to most other archaeal Lrp-like TFs [10–16] and to bacterial Lrp-like regulators that invariably bind amino acids. Known archaeal Lrp-like TFs have regulon sizes ranging from one or a few targets to a large number of genes and operons. Examples of the former are LrpA from Pyrococcus, LrpA1 from Halobacterium salinarum R1 , Ptr2 from Methanocaldococcus jannaschii[6, 7] and LysM from Sulfolobus solfataricus that has an intermediate number of target genes . Examples of the latter are FL11 from Pyrococcus OT3 , Lrp from H. salinarum R1  and Sa-Lrp from Sulfolobus acidocaldarius.
Lrp-like proteins generally have low sequence identities, but are structurally highly conserved . Typically, an Lrp monomer has a molecular mass of about 15 kDa and consists of two domains: an amino-terminal DNA-binding domain with a helix-turn-helix (HTH) motif and a carboxy-terminal domain, named Regulation of Amino acid Metabolism (RAM) , which is responsible for protein multimerization and cofactor binding . This RAM domain forms an αβ sandwich fold having an antiparallel β sheet composed of four strands “sandwiched” between two α helices. It has been observed that, in vitro, archaeal Lrp-like proteins associate into several multimeric forms via β strand exchange in the RAM domain [10, 17, 21–28]. Oligomerization is a prerequisite for formation of the cofactor binding pocket. Furthermore, cofactor binding induces conformational changes that in turn could affect oligomerization [11, 13].
In S. solfataricus, a crenarchaeal model organism, three Lrp-like TFs have been studied experimentally: LysM [10, 16], Ss-Lrp  and Ss-LrpB [9, 27, 29], the latter being one of the best characterized Lrp-like regulators in archaea. Ss-LrpB performs both positive and negative autoregulation in a concentration-dependent manner . Moreover, gene expression analysis in an Ss-lrpB deletion strain demonstrates that Ss-LrpB acts as an activator on its neighbouring target operon/genes encoding a pyruvate ferredoxin oxidoreductase (porDAB) and two putative permeases (Sso2126, Sso2127) .
At its target promoter regions, Ss-LrpB binds either a single or multiple, regularly spaced, binding sites harbouring a conserved motif [9, 29]. Each binding site is contacted by an Ss-LrpB dimer . In the control region of its own gene (Sso2131), three Ss-LrpB dimers bind cooperatively to juxtaposed sites . Occupation of all three sites results in strong DNA deformations and even DNA wrapping . Based on the 15-bp palindromic consensus sequence 5'-TTGCAAAATTTGCAA-3', the sequence specificity of Ss-LrpB binding was analyzed by saturation mutagenesis .
Despite extensive knowledge of the in vitro DNA-binding properties of Ss-LrpB, nothing is known yet about its in vivo binding behaviour and furthermore, it is unclear whether Ss-LrpB is a local or global acting TF. In this work, we investigate Ss-LrpB binding in an in vivo context by performing chromatin immunoprecipitation combined with DNA microarray analysis (ChIP-chip). Besides merely identifying in vivo binding sites, we perform an extensive comparative analysis of in vitro, in vivo and in silico binding, exploiting the knowledge of the DNA-binding specificity model. By combining in vivo binding data with gene expression analysis, we provide new insights into the biological functions of Ss-LrpB, which go beyond direct transcription regulation.
Strains and culture conditions
S. solfataricus P2 (DSM1617), PBL2025  and Ss-lrpB::lacS strains were grown aerobically at 80°C in Brock basic medium supplemented with 0.1% tryptone as a carbon and energy source . Escherichia coli strain DH5α was used for all cloning and plasmid propagation purposes. E. coli strain BL21(DE3) was used as a host for protein overexpression.
Each chromatin immunoprecipitation (ChIP) sample was prepared from a 200 ml culture of S. solfataricus P2 at mid-exponential growth phase. The entire ChIP procedure, from collecting cells to obtaining amplified enriched and input DNA ready to use for microarray hybridization was performed as described . In contrast to our previous work, in which a single ChIP sample was analyzed , we prepared and analyzed three biological replicate Ss-LrpB-specific ChIP samples. Prior to microarray hybridization analysis, samples were analyzed for enrichment relative to input DNA, which is total DNA extracted before immunoprecipitation, by quantitative PCR (qPCR) with primers specific for the Ss-lrpB control region (Additional file 1: Dataset S1). Furthermore, after ChIP-chip, enrichment of newly discovered binding regions was quantified similarly by qPCR. All primers are listed in Additional file 2: Table S1. qPCR was performed with a My-iQ Single Colour Real-Time PCR System (Bio-Rad) as described before , in triplicate and normalized to reference DNA, a non-related sequence fragment amplified from E. coli gDNA and spiked at 30 ng/sample before sonication.
Microarray hybridization and data analysis
Microarray hybridizations were performed with customized 385 K high-density tiling arrays by NimbleGen (Roche) as described previously . ChIP input and output samples were labelled with Cy3 and Cy5, respectively. Each probe occured twice on each array, yielding technical duplicate measurements for all samples. Microarray data analysis was performed using an extended version of the program described by Toedling and Huber , which uses the Ringo package of R-Bioconductor. The source code of the extended program is available via http://micr.vub.ac.be. It includes importing the data, data quality assessment, preprocessing of the data and identifying ChIP-enriched regions in a similar way as described in , with a threshold of 1 on the normalized log2 ratios. ChIP-enriched regions were selected as being co-associated when a LysM binding region overlapped at least partially with an extended Ss-LrpB ChIP-enriched region.
Binding motif predictions
Using a binding energy based position weight matrix of Ss-LrpB , binding motifs were predicted over (i) the entire S. solfataricus P2 genome sequence, (ii) the genomic regions comprising 200 bp upstream of the ORF start, and (iii) the ChIP-enriched regions. The latter was also performed using the MEME suite . Corresponding theoretical binding dissociation equilibrium constants (KDs) were calculated as well.
Genomic DNA (gDNA) was prepared from a 10 ml S. solfataricus P2 culture grown until late exponential growth phase as described before . Plasmid DNA was extracted from E. coli DH5α strains using a miniprep kit (Qiagen). For cloning of promoter regions, PCRs were performed with the FastStart High Fidelity PCR System (Roche Applied Sciences), S. solfataricus gDNA as a template and oligonucleotides (Sigma-Aldrich) as listed in Additional file 2: Table S1. In case of the gpT-1/mtaP promoter region, the oligonucleotides contained BamHI and PstI restriction sites, allowing subsequent cloning into the ampicillin resistant vector pUC18 . In case of the Sso0049 promoter region, the fragment was cloned into the pCR2.1-TOPO vector by using a TOPO TA cloning kit (Invitrogen). Individual binding sites were cloned in pBend2 by annealing two complementary oligonucleotides and ligating them into XbaI-restricted vector.
Electrophoretic mobility shift and footprinting assays
Recombinant non-tagged Ss-LrpB and LysM were overexpressed in E. coli BL21(DE3) and purified as described previously [16, 27]. Electrophoretic Mobility Shift Assays (EMSAs) were performed with gel-purified 5’-end 32P-labelled PCR fragments generated by using ReadyMix Taq PCR Mix (Sigma-Aldrich). For validation of in vitro binding to in vivo identified binding regions, S. solfataricus P2 gDNA was used as a template, whereas for study of in vitro binding to the promoter regions and individual sites of mtaP and Sso0049, plasmid DNA was used as a template. The sequences of all oligonucleotides (Sigma-Aldrich) are provided in Table S1 (Additional file 2). The experiments were performed as described previously  using LrpB binding buffer . The KD value for binding to the CRISPR4 target was obtained using the Densitometric Image Analysis Software, available at http://micr.vub.ac.be. DNase I and ‘in gel’ copper-phenantroline (Cu-OP) footprinting assays were executed as described [22, 29]. Reference ladders were generated by chemical sequencing .
Quantitative reverse transcription PCR (qRT-PCR)
For qRT-PCR analysis, 2 ml of an exponentially grown S. solfataricus PBL2025 or Ss-lrpB::lacS culture was mixed with 4 ml RNAprotect Bacteria Reagent (Qiagen) and centrifuged. Pelleted cells were subsequently lysed and RNA was extracted with the SV Total RNA Isolation System (Promega). To prevent gDNA contamination, RNA samples were treated with DNase I using the TURBO DNA-free kit (Invitrogen) according to manufacturer’s instructions. First-strand cDNA was synthesized from 1 μg RNA with SuperScript III First-Strand Synthesis SuperMix kit (Invitrogen). Primers (Additional file 2: Table S1) were designed with Primer3 software  and purchased at Sigma-Aldrich. qPCR was performed in a Bio-Rad iCycler with each reaction mixture containing 12.5 μl iQ SYBR Green Supermix (Bio-Rad), forward and reverse primers and 1 μl 100-fold diluted cDNA. The amplification protocol was as follows: initial denaturation at 95°C during 3 minutes, 40 cycles of 95°C during 10 seconds and 55°C during 30 seconds and one cycle of 95°C during 1 minute and 55°C during 1 minute. The melt curve analysis demonstrated absence of primer-dimer formation. All qRT-PCR assays were carried out in technical duplicate for at least four biological replicates and with a no-template and a no-RT control. Quantification cycles (Cqs) were determined with Bio-Rad iQ5 software and mean relative gene expression ratios including standard deviations were calculated with the 2(−ΔΔCt) method . Normalization was with respect to the expression of tbp, which was comparable in both strains. Data were subjected to t-test analysis using the statistical package Prism 6.0 (GraphPad).
Genome-wide binding profile of Ss-LrpB
The genes that are overlapping or closest to the 37 ChIP peaks belong to various functional classes such as amino acid metabolism, energy metabolism, central metabolism and transport (Additional file 4: Figure S2). We also classified peaks according to their location with respect to open reading frames (ORFs) (Figure 1B and C; Additional file 1: Dataset S1). Although the majority of identified ChIP peaks are overlapping or located in an intergenic region (81%), many of these locations are unusual for a typical TF and are distant from promoters, as also demonstrated by the large distances between most peak centers and the closest experimentally determined transcription start sites (TSSs)  (Additional file 1: Dataset S1). Several peaks, classified as “overlap” , (partially) cover two or more ORFs (Additional file 5: Figure S3).
In vitro analysis of Ss-LrpB binding to in vivoidentified binding regions
It is notable that most of the newly identified Ss-LrpB binding sites that are directly bound, both in vitro and in vivo, are located in a highly variable region of the genome comprising multiple insertion sequence (IS) elements (Figure 1A). Furthermore, most of these Ss-LrpB binding sites are in the direct neighbourhood of an IS element. For all 12 in vitro bound Ss-LrpB targets, binding occurs with a lower affinity as compared to the control region of the Ss-lrpB gene itself  (Figure 2A). For example, the CRISPR4 target, one of the higher-affinity targets, is bound with an equilibrium dissociation constant (KD) of 63 ± 5 nM. The predictive power of the binding energy weight model appears to be limited as several predicted binding motifs have low theoretical KDs but nevertheless did not exhibit a specific interaction in vitro and vice versa, as several specifically bound motifs have high theoretical KDs (Additional file 7: Table S2).
In vivo binding to Ss-lrpB, porDAB, Sso2126 and Sso2127operators
An active copy of ISC1078 (containing a transposase encoded by Sso2132) is located downstream of Ss-lrpB with respect to genome sequence orientation [9, 43]. However, the steep decline in ChIP enrichment for the probes representing the concerned IS sequence (Figure 3) and further PCR analysis (Additional file 8: Figure S5) demonstrated the absence of this element in a large subpopulation of the cells that were subjected to ChIP.
Interestingly, the EMSA screen also resulted in a further unraveling of the Ss-lrpB operator for autoregulation: a fragment comprising the sequence between the insertion site of ISC1027 and the Sso2133 (glpK-2) promoter results in the formation of a single specific complex (Figure 2A, Sso2133 target). This observation implies sequence-specific recognition of another Ss-LrpB binding motif, located downstream (with respect to genome sequence orientation) of Box3 with a center-to-center distance of 174 bp (Additional file 9: Figure S6). Possibly, this site, referred to as Box6, is an auxiliary operator site that supports binding to the main high-affinity operator sites, besides the intragenic Box4 and Box5, which were identified previously as secondary operator sites (Additional file 9: Figure S6) .
Ss-LrpB binds in CRISPR A and B leader regions
Given the high sequence identity between the CRISPR A and B leader regions, it is assumed that binding occurs at the corresponding site with the same sequence in the CRISPR A leader (Figure 4D). The center of the identified Ss-LrpB binding site is located 116/117 bp upstream of the main pre-crRNA TSS  in the first CRISPR repeat sequence, which is preceded by a strong promoter. This leads us to hypothesize that Ss-LrpB affects transcription of pre-crRNA through interaction with the basal transcription machinery.
Permissivity of the genome for Ss-LrpB binding
Using the binding energy position weight matrix, we searched the entire S. solfataricus P2 genome for additional potential Ss-LrpB binding motifs. Setting the threshold for the theoretical KD at 14 μM, a value still significantly lower than the average theoretical KD calculated for the novel identified Ss-LrpB sites that are bound both in vivo and in vitro (290 μM), we detected 519 motifs of which 100 are located in regions 200 bp upstream of translational starts (Additional file 10: Table S3). Some of these motifs are canonical Ss-LrpB binding motifs located at appropriate distances from promoters to have the ability to exert regulation. Nevertheless, binding is absent at these locations in vivo.
A similar discrepancy between in vitro and in vivo binding was shown to exist for the intergenic promoter region shared between the divergently transcribed Sso2342, encoding a purine phosphoribosyltransferase (gpT-1), and Sso2343, encoding a 5’-methylthioadenosine phosphorylase (mtaP) (Additional file 11: Figure S7; Additional file 12: Figure S8; Additional file 13: Figure S9). Altogether, these observations demonstrate that the target DNA sites within the S. solfataricus genome are not entirely permissive for Ss-LrpB binding.
Overlap between Ss-LrpB and LysM binding profiles
Overview of the Ss-LrpB-specific ChIP-enriched regions that are also enriched in LysM-specific ChIP-chip analysis
Genomic coordinate peak start
Genomic coordinate peak stop
Relative position to LysM-specific peak
Presence of Ss-LrpB binding motif
Presence of LysM binding motif
overlap (93% )
The known regulatory targets of Ss-LrpB, Sso2126, Sso2127 and porDAB, are not co-bound by LysM, in contrast to most of the newly discovered low-affinity Ss-LrpB binding targets. Conversely, the main local regulatory target of LysM, the lysWXJK operon for lysine biosynthesis, is also bound by Ss-LrpB. To distinguish between (i) a mutually exclusive binding of either Ss-LrpB or LysM at a particular target in a subpopulation of cells, or (ii) a situation where the proteins bind as a co-complex to this target, we compared in vitro and in silico binding characteristics for these targets (Table 1). Shared binding regions could be categorized in three classes: (i) binding regions that contain an Ss-LrpB binding motif and exhibit binding to Ss-LrpB but not LysM in vitro (class I); (ii) binding regions that contain a LysM binding motif and exhibit (predicted) binding to LysM but not Ss-LrpB in vitro (class II) and (iii) binding regions that do not contain an Ss-LrpB or LysM binding motif and do not interact with any of the two proteins in vitro (class III). There is a perfect inverse correlation pattern between the presence of a bona fide Ss-LrpB or LysM binding motif suggesting that the TFs are co-localized through protein-protein interaction and that only one of the protein partners directly interacts with the DNA.
We further investigated the simultaneous interaction of LysM and Ss-LrpB to one of the co-bound targets, Ssot28, in an in vitro assay (Figure 6C). Whereas Ss-LrpB does not form specific complexes with this target (Additional file 6: Figure S4), LysM forms a single complex by binding to a site located close to the promoter of the glutamate synthase (gltB) gene . EMSA analysis demonstrated that the addition of Ss-LrpB to reaction mixtures containing LysM and the DNA stimulated slightly the complex formation (Figure 6C). Furthermore, the addition of Ss-LrpB-specific antibodies resulted in a clear supershift of the complex. These observations suggest that Ss-LrpB is present in the nucleoprotein complex, despite that it is unable to interact with the DNA fragment by itself.
Gene expression analysis of transcripts associated with Ss-LrpB binding regions
The expression of CRISPR B pre-crRNA is moderately downregulated (2-fold) in the Ss-lrpB::lacS strain in comparison to the isogenic WT, indicating an Ss-LrpB-mediated activation. It is assumed that a similar regulation will be exerted at the CRISPR A locus. In conclusion, Ss-LrpB is involved in CRISPR regulation whereas the other identified binding events appear to occur without apparent regulation under our conditions of growth.
Our genome-wide localization study has revealed the association of Ss-LrpB with at least 37 loci in the S. solfataricus genome. A subset of these in vivo Ss-LrpB-targeted sites is clearly validated to be bound with this pure TF in vitro and to contain a bona fide sequence motif. Obviously, the well-established, high sequence specificity of Ss-LrpB  is responsible for the complex formation at these sites. However, computational analysis with a binding energy based position weight matrix of the S. solfataricus genome demonstrated a vast overrepresentation of appropriate sequence motifs of which only a very small subset seems to be actually bound in vivo. A high number of false negative signals in the ChIP-chip analysis, e.g. due to a too stringent threshold, is a possible explanation for the observed lack of in vivo binding at predicted motifs. However, a closer investigation of the binding profiles at loci containing multiple high-affinity binding motifs (promoter regions of Sso0049 and mtaP) confirmed complete absence of Ss-LrpB-specific enrichment in these regions. Therefore, we conclude that the intrinsic DNA-binding sequence specificity is a poor predictor of binding in vivo under our culture conditions of S. solfataricus and that additional factors are involved in determining site selectivity and occupancy in vivo.
In vivo binding site selectivity could be influenced by the structural landscape of the chromatin that imposes differential genome accessibility on a higher organizational level. Similarly to bacteria, Crenarchaeota have their nucleoid structurally organized by small chromatin proteins , however it is unknown how this genome packaging affects TF binding. Possibly, Ss-LrpB binding is restricted by the action of nucleoid associated proteins resulting in differential accessible genomic regions. Apparently, accessibility is facilitated in a highly variable region of the S. solfataricus genome with multiple IS elements and low abundance of essential genes. However, there are alternative explanations available for the lack of association at high-affinity binding motifs. For instance, it might be caused by the presence of a co-repressor, ligand or post-translational modification that inhibits Ss-LrpB binding under the used culture conditions or, on the contrary, by the absence of a critical ligand in vivo that activates binding and was present in the in vitro binding reaction mixtures, possibly through co-purification after heterologous expression of Ss-LrpB in E. coli. Technical details, such as unstable behavior of the TF-DNA complexes during formaldehyde fixation or sonication, could also lead to certain binding events not being detected.
There is a very low correlation between Ss-LrpB binding and transcription regulation, which appears to be limited to the Sso2126, Sso2127 and porDAB gene targets that were identified previously and to the CRISPR A and B clusters. Furthermore, a significant fraction of binding regions is located at a significant distance from the nearest TSS and associated promoter and/or is even intragenic, an observation made for bacterial [46–49] and for archaeal TFs as well [16, 50]. The newly discovered genomic targets that contain a binding motif are generally contacted by Ss-LrpB through non-cooperative low-affinity binding, in contrast to the local gene targets. Low-affinity binding without apparent regulation appears to be universal as it has been observed repeatedly for TFs of M. tuberculosis, yeast  and Drosophila. However, the biological function of these weak binding sites is still unclear. It has been hypothesized that they could display a subtle regulatory activity, which goes undetected and causes a fine-tuning of gene regulation. In this way, these binding sites alleviate selective pressure on specific loci, namely classical regulatory binding sites, and increase the ability to evolve . An alternative explanation for the biological role of these low-affinity sites is that they might create a biological buffering system that serves as a reservoir to sequester TF molecules, thereby thermodynamically regulating the concentration of freely available protein [53, 54]. This could be a critical factor for correct functioning of Ss-LrpB, given the limited number of target genes and the cooperative nature of the interaction at these targets.
The observed occupancy levels and regulatory effects of the major regulatory targets (Sso2126, Sso2127, porDAB and CRISPR B) are weak and were most probably recorded in a growth condition yielding a non-regulated “ground state” . Possibly, a different, as yet unknown, culturing condition leads to higher occupation and corresponding activation levels of the regulatory target genes. Similarly, some of the newly identified binding sites might mediate regulation of nearby genes under different growth conditions than those in which the binding profile was monitored. The exact functions and substrate specificities of the pyruvate ferredoxine oxidoreductase and the two permeases are unclear although it is tentative to speculate that these proteins function during lactate oxidation or a related metabolic pathway . Indeed, Sso2126 encodes a permease that exhibits homology with bacterial L-lactate permeases and Sso2127 codes for a homolog of halophilic oxalate/formate antiporters. Protein sequence analysis suggests that if Ss-LrpB is bound by an effector molecule, it is a small molecule other than an amino acid .
The growth condition that is more relevant for Ss-LrpB regulation might be a more stressful condition for the cells, given the Ss-LrpB-mediated CRISPR activation. It has been demonstrated that expression of CRISPR and CRISPR-associated (CAS) genes is inducible by abiotic and/or biotic stress . The high energetic cost of expressing the long pre-crRNA leads to hypothesize that it is under a complex transcriptional regulation involving multiple regulators, of which we demonstrate that Ss-LrpB is the first promoter-interacting TF to be characterized in S. solfataricus. Co-regulation of porDAB, Sso2126 and Sso2127 on one hand and the CRISPR clusters on the other hand indicates that there is a possible link between metabolic regulation and immunity defense in S. solfataricus.
Remarkably, the Ss-LrpB and LysM binding profiles display a significant overlap. The detection of the two proteins at the same genomic location in different ChIP-chip profiles could be explained by (i) binding of a hetero-protein complex of LysM and Ss-LrpB to the DNA target or (ii) a heterogeneous occupancy where Ss-LrpB is present on the DNA site in some cells, and LysM on the corresponding DNA site in other cells or (iii) a combination of both possibilities. In the case of these two Lrp-like TFs, the inverse correlation pattern between the presence of a LysM or Ss-LrpB binding motif suggests that they are simultaneously associated as a complex with the target DNA sites in the S. solfataricus genome. Furthermore, this co-association occurs most likely via protein-protein interactions in which only one of the protein partners interacts with DNA, rather than by cooperative interactions between distinct DNA-bound TF molecules. This proposal is supported by our in vitro experiments where Ss-LrpB was shown to be present in the protein-DNA complex although it does not interact with the DNA by itself. For a distinct class (“class III”) of binding targets, direct DNA recognition by Ss-LrpB or LysM was clearly absent, suggesting the involvement of additional TFs. Remarkably, we did not observe significant Ss-LrpB-mediated regulation of the major LysM targets to which co-association was observed. Possibly, the presence of Ss-LrpB results only in subtle regulatory effects, fine-tuning the regulatory action of LysM, and the involvement of different partners is partially interchangeable. Our data demonstrate that archaeal Lrp-like TFs interact in vivo, supporting previous data that Pyrococcus Lrp-like TFs tend to form hetero-oligomeric structures in vitro[11, 17].
In conclusion, our genome-wide association study of Ss-LrpB yields novel insights into its in vivo interactions despite providing only limited additional information on its physiological role. Ss-LrpB interacts with multiple low-affinity sites throughout the genome without an apparent regulatory purpose and these sites are often associated with IS elements. Furthermore, the TF binds in the CRISPR A and B leader regions and activates expression of pre-crRNA. Ss-LrpB also co-associates with another Lrp-like TF, LysM. Hetero-oligomerization of archaeal Lrp proteins was previously observed in vitro and thus, we provide the first indications of an interplay of two of these factors in vivo. Finally, the absence of Ss-LrpB in vivo on sites carrying a well-predicted binding motif suggests a limited permissivity of the S. solfataricus genome for association with its cognate TF.
All the supporting data are included as additional files.
The authors are grateful to Phu Nguyen Le Minh for the gift of purified PepA protein. This work was supported by the Research Foundation Flanders (FWO-Vlaanderen) (a postdoctoral fellowship to EP and a pre-doctoral fellowship to LvO), the Flemish Institute of Biotechnology (Vlaams Instituut voor Biotechnologie (VIB)), the Research Council of the Vrije Universiteit Brussel, the Vlaamse Gemeenschapscommissie, the China Scolarship Council (CSC) and the European Union (EU) (project 241481 (AFFINOMICS) to SM).
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