- Research article
- Open Access
Genomic tools development for Aquilegia: construction of a BAC-based physical map
- Guang-Chen Fang†1,
- Barbara P Blackmon†2,
- David C Henry1,
- Margaret E Staton2,
- Christopher A Saski2,
- Scott A Hodges3,
- Jeff P Tomkins2 and
- Hong Luo1Email author
© Fang et al; licensee BioMed Central Ltd. 2010
- Received: 26 May 2010
- Accepted: 8 November 2010
- Published: 8 November 2010
The genus Aquilegia, consisting of approximately 70 taxa, is a member of the basal eudicot lineage, Ranuculales, which is evolutionarily intermediate between monocots and core eudicots, and represents a relatively unstudied clade in the angiosperm phylogenetic tree that bridges the gap between these two major plant groups. Aquilegia species are closely related and their distribution covers highly diverse habitats. These provide rich resources to better understand the genetic basis of adaptation to different pollinators and habitats that in turn leads to rapid speciation. To gain insights into the genome structure and facilitate gene identification, comparative genomics and whole-genome shotgun sequencing assembly, BAC-based genomics resources are of crucial importance.
BAC-based genomic resources, including two BAC libraries, a physical map with anchored markers and BAC end sequences, were established from A. formosa. The physical map was composed of a total of 50,155 BAC clones in 832 contigs and 3939 singletons, covering 21X genome equivalents. These contigs spanned a physical length of 689.8 Mb (~2.3X of the genome) suggesting the complex heterozygosity of the genome. A set of 197 markers was developed from ESTs induced by drought-stress, or involved in anthocyanin biosynthesis or floral development, and was integrated into the physical map. Among these were 87 genetically mapped markers that anchored 54 contigs, spanning 76.4 Mb (25.5%) across the genome. Analysis of a selection of 12,086 BAC end sequences (BESs) from the minimal tiling path (MTP) allowed a preview of the Aquilegia genome organization, including identification of transposable elements, simple sequence repeats and gene content. Common repetitive elements previously reported in both monocots and core eudicots were identified in Aquilegia suggesting the value of this genome in connecting the two major plant clades. Comparison with sequenced plant genomes indicated a higher similarity to grapevine (Vitis vinifera) than to rice and Arabidopsis in the transcriptomes.
The A. formosa BAC-based genomic resources provide valuable tools to study Aquilegia genome. Further integration of other existing genomics resources, such as ESTs, into the physical map should enable better understanding of the molecular mechanisms underlying adaptive radiation and elaboration of floral morphology.
- Anthocyanin Biosynthesis
- Average Insert Size
- Contig Assembly
- Comparative Genomic Study
- Minimal Tiling Path
Recent progress in genomic research using the model species A. thaliana and crop species, such as rice, maize, sorghum and tomato, has dramatically enhanced our capacity to unravel the genetic basis of biological diversity and the evolution of complex traits and genetic pathways in plants. These include genes and pathways determining plant architecture and fruit size [1, 2], flowering time [3–6], light response [7, 8] and plant defence . However, when studying fundamentals about how organisms have adapted to their natural environments, the information derived especially from crops is of limited application. In these species, many of the traits have undergone intensive artificial selection over the course of directed genetic improvement. In addition, these plant species are often highly inbred due to either artificial selection or self-pollination, which may lead to an increased likelihood of accumulating deleterious alleles, and consequently the loss of atypical patterns of much of the genetic variation displayed in natural systems [10, 11]. It is, therefore, critical to identify and develop new model species from natural settings, with well-defined ecologies and abundant examples of adaptation to various environments to advance our understanding of plant evolution, development and ecology.
Several important factors need to be carefully weighed when choosing new model species to develop tools for genomic studies [12, 13]. First, it is desirable that the new systems encompass a wide range of morphological and ecological diversity occurring in the flowering plants. This will not only allow understanding of the morphological variation in response to critical phenomena, but also facilitate investigation of the physiological adaptation to new environments. Second, with the information accumulated from the increasing number of model systems within grasses (e.g., rice, maize, sorghum, and Brachypodium distachyon) and core eudicots (e.g., Arabidopsis, Medicago, tomato, and Mimulus), the development of phylogenetically intermediate model systems would greatly facilitate genome comparisons among these taxa and bridge the deep evolutionary distance between the well-developed Arabidopsis and rice model systems [13, 14]. Third, it is ideal that genetic resources developed for new model systems could be transferable to a wide-range of related species, serving to address many questions for the community at large .
Aquilegia, the columbine genus , is emerging as a new evolutionary genomic model in a relatively unstudied area of the plant phylogenetic tree, the basal eudicots. Aquilegia species are so closely related that they have been considered a species flock or syngameon [15, 16]. This group was extensively studied by Verne Grant as an interesting example of recent adaptive radiation leading to rapid speciation . Variation in pollinators has apparently driven the evolution of a wide variety of floral morphologies and, along with adaptation to different habitats, likely induced rapid reproductive isolation [16, 17]. Approximately 23 different species in North America emerged in as little as 1-3 million years  resulting in little sequence variation in DNA regions such as chloroplast and rDNA . Remarkably, ecologically and morphologically distinct species share the majority of sequence polymorphisms making them difficult to distinguish at the molecular level [17, 19–21], which again suggests that these taxa are of very recent origin. As such, it is not surprising that it has long been known that species of Aquilegia are highly cross-compatible  which provides an opportunity for genetic studies across multiple fields such as ecology, physiology and morphology [11, 16, 22, 23]. The feature of cross compatibility not only facilitates the genetic dissection of traits, but also suggests that genomic tools developed from a single species will be readily transferred to a wide range of additional species .
Phylogenetically, Aquilegia belongs to the plant family Ranunculaceae, which is a basal-eudicot lineage [23, 25]. This position of being approximately equidistant, evolutionarily, from the current monocot (rice) and core-eudicot (Arabidopsis) model plant systems provides a unique opportunity for comparative studies among angiosperms of sequence information, genome structure, and the conservation/diversification of developmental pathways. For instance, it has been hypothesized that a whole-genome duplication occurred near the base of the eudicot lineage yet Aquilegia, and thus the Ranunculales, appears to have predated this event [26–28]. Moreover, Aquilegia possesses unusual floral morphology such as petaloid sepals, nectar spurs and a recently evolved novel floral organ, the staminodium, not available for study in current model systems . These traits, along with its small genome size (~300 Mb), all support Aquilegia as an important new model for plant development, ecology and evolution.
Here, we report the construction of a physical map of the A. formosa genome using the High Information Content Fingerprinting (HICF) method . A single individual from the wild was used for library construction because inbreeding depression has been found to be exceptionally strong in all species of Aquilegia studied to date . This plant has also been used as a grandparent in a large F2 cross between A. formosa and A. pubescens, which was utilized as the tissue source for a large EST database http://compbio.dfci.harvard.edu/cgi-bin/tgi/gimain.pl?gudb=aquilegia and is being used for QTL mapping. In addition, we integrated a total of 197 markers (many derived from the EST database) by multi-dimensional pool hybridization, and produced BAC end sequences of a minimum tiling path. Thus, the physical map described here integrates across a number of studies and genomic resources for Aquilegia. The physical map and other information presented here will help facilitate the long-range assembly of Aquilegia genome sequence and fine-scale mapping of QTLs as well as comparative genomic studies. Because all species of Aquilegia are highly similar at the genetic level (see above), this resource should be important for genomic studies spanning the entire genus.
BAC library characterization
Summary of BAC libraries and HICF fingerprinting of BAC clones
Average insert size (kb)
Valid bands per clone (average)
# of clones fingerprinted
Summary of the Aquilegia physical map constructed by HICF
Number of clones successfully fingerprinted
Number of clones in Physical Map
Number of clones in contigs
Size of contigs
Number of contigs
Physical length of the contigs (Mb)
Physical Map Assembly
Fingerprints from both BAC libraries were combined for contig assembly using a tolerance of 3 and a cutoff of 1e-50. The initial assembly resulted in 3,444 contigs containing 39,489 (78.7%) clones and 10,674 singletons. Eight clones were manually removed due to poor fingerprinting. The average Sulston score was 0.879. After using the DQer to break up contigs consisting of more than 10% questionable clones, a consecutive reduction in stringency at 1e-5 for each End-End and Single-End merge using the "clone plus markers (CPM)" function at a tolerance of 3 was performed to reassemble contigs. The final systematic contig assembly was calculated at a cutoff value of 1e-35. Further manual contig merges, based on the results from marker hybridization (as described in "Methods"), were conducted at a cutoff value of 1e-20 and tolerance of 3, and resulted in a total of 832 contigs consisting of 46,216 (92.1%) clones and 3,939 singletons (Table 2). A minimal tiling path (MTP) was selected using default parameters of FPC and 6,505 clones were selected for end sequencing.
Overgo probe hybridization and marker integration
Statistics for hybridization with drought-stress induced markers
Total number of markers used
Number of markers failed
Number of markers hybridized to single contig
Number of markers hybridized to two contigs
Number of markers hybridized to more than 2 contigs
Summary of the contigs anchored to different linkage groups by mapped genetic markers
Aquilegia physical contigs mapped by two or more genetic markers from the second pool hybridization
Map position (Linkage group/cM)
TC1022, TC9132, TC15023
3/75.7 - 80.3
4/50.3 - 51.3
2/33.5 - 40.3
4/50.3 - 51.3
Aquilegia physical contigs mapped by 16 markers potentially involved in anthocyanin biosynthesis
Contig (length in kb)
Map position (Linkage group/cM)
Validation of the contig assembly
Verification of FPC contig assembly by PCR amplicons designed hybridization markers.
# positive hits (# contigsa)
# positive hits with expected amplicons
mlp-like protein 28
Fwd AGGTGATGGAACCTGTGAGG Rev CACAATCCATGTCACCAAGC
14 (3 +3sb)
Fwd GGCTATATCCACCAGGCTGA Rev AAGGGCCAGCACTTTATCCT
Late embryogenesis-abundant protein
Fwd ATCATCCAACCTTGCGTTGT Rev GGGACCGGAACTATCCAAAT
Ubiquitin-conjugating enzyme E2
Fwd GCCCAAATCAAGAAACCAGA Rev CCTTTATGGACCCTGGATCA
putative staygreen protein
Fwd TGGGGTCCACTTAAAGATGC Rev GAGTTGGTTGGTTTGGTTCC
universal stress protein 1
Fwd AGTAACTGGGCAAGCAGCAT Rev ATGGTGATGCAAGGGAAAAA
ethylene-responsive transcriptional co-activator)
Fwd ATCGCATCGTCATCAAACAA Rev TTCAGCAGGCGTACGACGAG
Fwd ACACTACGACATGCCAACCA Rev TAGCCCAGCCCAACAAATAG
BAC end sequencing
BAC end sequencing from both forward and reverse directions of 6,505 BACs covering a minimal tiling path of the physical framework generated a total of 12,086 (93% success) high quality sequences (at least 100 contiguous bases ≥ phred20) with an average length of 567 bases. This was equivalent to one sequence tag per 24.8 kb (considering the genome size of 300 Mb). After filtering for vector contamination and trimming for quality, the BESs were deposited in GenBank's GSS sequence repository: library AF_Bb has accessions ER936645-ER942217 and library AF_Bc has accessions ER967023-ER973759. Comparison of the BESs to multiple plant chloroplast and mitochondrial genomes indicated a low level of plastid-origin BACs, 0.2% for each organelle. The BAC sequences, excluding those of putative plastid origins, encompass 6,834,517 base pairs, which corresponds to approximately 2.3% of the Aquilegia genome .
The BAC end sequences have an average GC content of 37.6%. Microsatellites were identified from 2,091 BESs, and primers could be designed to flank 1,630 of the SSRs. These putatively mappable markers include 570 dinucleotide repeats, 550 trinucleotide repeats, 525 tetranucleotide repeats, and 177 pentanucleotide repeats.
A set of 1,729 sequences matched known transposable elements. Considering only the best matching element for each BAC end sequence, the most commonly matched species from the database was grapevine (V. vinifera) with 414 BAC ends having matches to grapevine elements. The matches encompassed 66 different repetitive elements including multiple members of the gypsy, copia, MuDR and En/Spm classes. These matches had an average Smith-Waterman score of 682. The next most common organisms for matches consisted of A. thaliana (370 BAC ends to 76 elements, score of 662), Populus trichocarpa (296 BAC ends to 43 elements, score of 878), followed by Medicago truncatula (166 BAC ends to 59 elements, score of 577), and Oryza sativa (143 BAC ends to 57 elements, score of 430).
The most commonly identified individual element was Atlantys1_1 that matched 143 A. formosa BAC ends. Atlantys1_1 represents an internal coding segment of the larger Atlantys endogenous retrovirus in the Ty3-gypsy family. This family is widespread across plants; Atlantys accounts for much of the genome size variation in rice  and also has RepBase records originating from A. thaliana, Lotus japonicus, and Sorghum bicolor. Other commonly identified elements include copia42-PTR_I, an LTR retrotransposon from Populus matching 83 BAC ends; POPGY1_I, the internal portion of a Gypsy-type retroelement matching 64 BAC ends; and Copia-31-lTR_VV, a LTR retrotransposon from V. vinifera matching 60 BAC ends (Additional file 1)
After filtration of organelle, transposable elements and repetitive sequences, the remaining BESs were assembled with CAP3  followed by mining for potential gene coding regions. The assembly resulted in 8,140 singlets and 458 contigs. Two different strategies were used to identify potential coding regions in the unique sequences. In the first approach, the non-redundant dataset was compared to the tentative Aquilegia consensus EST sequences from the Gene Index Project  with tblastx  at a stringency of 1e-25. The results indicated 2,488 of the genomic sequences have at least one EST match. As the EST resource represented only an imperfect representation of the transcriptome, a BLASTX of Arabidopsis, Oryza and Vitis gene models was further performed with a cut-off E value of 1e-25 in the second approach, resulting in an additional 750, 337 and 921 potential coding non-redundant BAC ends, respectively. Of the 8598 non-redundant sequences, 2,782 (23% of the total 12,086 BESs) were flagged as potential coding regions.
Comparative mapping of Aquilegia and V. vinife r aa
Number of Aquilegia BESs
Aquilegia represents a unique clade of basal eudicots possessing a number of important unique features, including its phylogenetic position in the lower eudicots, unusual floral morphology (e.g., petaloid sepals, nectar spurs and staminodia), and its distribution in diverse ecological habitats. Collectively, all these traits contributed to Aquilegia being developed as a new model system for studying floral variation, adaptive radiations and evolution [23, 32, 36]. To further understand the genome structure and provide molecular insights bridging monocots and eudicots and facilitate molecular dissection of the traits associated with inflorescence development and environmental adaptations, a BAC-based genomic resource, including three BAC libraries and a physical map, was developed in this study. Among the three libraries were two libraries derived from A. formosa, representing 15.2X and 13.3X genome equivalents, respectively, for physical map construction. A third library was constructed from A. coerulea Goldsmith to have 20.7X genome coverage for further comparative genomics studies to address the molecular basis for floral variation and adaptive radiation within the genus. The Aquilegia physical map was composed of 50,155 clones and had a deep 21X genome coverage. Furthermore, a collection of BACs orchestrating a minimal tiling path from the contig assembly were isolated for BAC end sequencing to provide a glimpse of the genome organization of this model plant. Both the physical map and the BESs could also serve as landmarks for genome sequence assembly and anchoring ESTs to the genome. Marker hybridizations using a total of 197 markers associated with drought-stress, anthocyanin biosynthesis and floral development not only allowed integration of genetic map into the contig framework, but also identified candidate genomic regions for further gene isolation and characterization. The genome resource is expected to serve as a pivotal platform for comparative genomics study to elucidate genome variations between monocots and basal eudicot and to provide insights into the molecular mechanisms underlying environment adaptation and floral variations.
In recent years, HICF fingerprinting has been commonly applied to replace traditional agarose  and polyacrylamide gel methods  in various genome fingerprinting projects due to its high-throughput procedure, increased number of fragments generated from each clone and more improved contig assembly than other approaches . In this study, an average of 81 restriction fragments was generated from the clones in the FPC project. The high-informative fingerprints provided high resolution identity from each clone for accurate contig assembly that can be further verified by marker hybridization in which 189 (96%) of the total 197 genetic markers hybridized to only 1 or 2 contigs instead of scattering around the entire genome. Furthermore, the positively hybridized clones were overlapped in clusters in most contigs, indicating that the contig assembly, which is based on fingerprinting similarity, is consistent with the sequence-based results. The accuracy of contig assembly could also be verified by PCR amplicon analysis as shown in Table 7. Thus, we are confident with the strategy for building a physical map that begins with contig assembly at high stringency at cutoff 1e-50 and tolerance 3, which gave a high average Sulston score of 0.879, followed by a series of End-End and Single-End merges of the small BAC scaffolds under gradually decreased stringency till 1e-35, followed by further manual editing at 1e-20 based on marker hybridization data. Among the successful 197 markers used for the hybridization were 87 markers that have been genetically mapped; these markers anchored a total of 54 contigs that cover 76.4 Mb (25.5% of the genome) on all 7 linkage groups (Table 4). These mapped contigs not only organize a framework to study the Aquilegia genome, but also pave the way for gene isolation and characterization by map-based cloning approach to further understand the genes of interest.
The genes involved in anthocyanin pigmentation biosynthesis in wheat are arranged in a gene cluster in the short arm of chromosome 7 [44–46]. Similar clustering of the genes involved in the biosynthesis of secondary metabolites was also reported from grapevine . Unlike these species, the 16 anthocyanin biosynthesis related genes in Aquilegia appear to be dispersed in the genome (Table 6), suggesting the unique deployment of the genes in this lower eudicot genus. However, a number of additional genes belonging to the anthocyanin and broader flavonoid pathway have been identified  but not assayed here, and therefore the possibility cannot be ruled out that some gene clustering might be identified in the future. The contigs anchored from this study could serve as resource for unravelling the molecular basis underlying floral color variation and evolution.
An expansion in the physical span of the contigs was observed in this study. The collective physical span of all contigs as calculated by the CB map function of FPC software  was estimated to be 689.8 Mb (~ 2.3X genome size, 1N = 300 Mb). As only 197 marker hybridization results were analyzed and these markers were biased toward specific biological functions, it cannot be ruled out, although unlikely, that the contig assembly is not best optimized and some contigs remain to be further merged together. As the single A. formosa individual used for BAC library construction has been shown to be highly heterozygous at more than 30 SSR and SNP loci (Hodges, unpublished data), the excessive physical length might be due to the heterozygous genome collected from the field that was composed of highly diverse haplotype DNAs as a result of the outcrossing nature of the species. Similar inflated length from physical map has been reported from other outcrossing species, including poplar  and grapevine . As the genome sequencing project is near finishing, further assembly and analysis of genome sequence will uncover more details about the genome components and suggest events that took place affecting genome structure of this basal eudicot taxa. To maintain the accuracy in contig assembly, further reduction in stringency to merge more contigs was not pursued in this study. In the future, fingerprint contig assembly can be refined through more hybridizations using additional mapped markers and probes designed from the end clones of contigs.
The BESs from the minimal tiling path clones also provided insights into the genome composition of this novel model plant, including low GC content, transposable elements and gene content. Interestingly, higher homology in putative coding regions shared between Aquilegia and the grapevine, V. vinifera, in comparison to two other model plants, including rice and Arabidopsis was also observed (Figure 5). As Vitis is affiliated with the earliest diverging lineage of rosids in the core eudicots of the angiosperms , and Aquilegia is in basal eudicots in the phylogenetic tree [23, 32], the close conservation between these two species not only provides a global molecular evidence to support the phylogenetic lineage that connects basal eudicots to core eudicots but also provides a rich resource for investigating the genome evolution, such as the events of genome duplication and subsequence variation [51–53], in the course from monocots to eudicots in angiosperms. In this report, preliminary comparative genomics studies using SyMAP uncovered 54 syntenic blocks between Aquilegia and Vitis (Figure 6). These syntenies provide a first glimpse of the Aquilegia structural organization and a rich resource to trace the events of DNA translocation during the evolution of these two lineages. Further characterization of the shared transposable elements from the Aquilegia genome will also provide insights into the evolution of plants. More extensive survey using the whole-genome sequence information in the near future is expected to aid in-depth studies into the evolution genomics of the basal eudicot taxa. On the other hand, the discovery that alignment of the BESs from the physical framework contigs failed to identify significant synteny with other reported genomes also reiterates the significance of the unique genome structure of Aquilegia in understanding the evolution of the plant genomes.
The BAC-based genome resource established from this study, including deep genome coverage libraries from A. formosa and A. coerulea, a partially integrated physical map is expected to promote better understanding of the genome structure of the unique intermediate between rice and Arabidopsis. It will also provide tremendous insights into the molecular clues and genetic networks underlying ecological adaption and morphological diversity. Results from the analysis of the BESs derived from the minimal tiling path (MTP) indicated a close similarity in both transposable elements and annotated gene models with the grapevine genome further suggesting the significance of the genome resource in studying the molecular elements involved in the lineage of evolution progression. This genomic resource is expected to facilitate comparative genomics research, gene isolation and characterization to address the unique biological feature of this novel model plant.
BAC DNA fingerprinting and contig assembly
DNA was isolated from a total of 58,368 clones from both AF_Bb and AF_Bc BAC libraries by following standard alkaline lysis miniprep methods , and used for fingerprinting using the HICF method of Luo et al.. The fingerprinting profiles were further processed by GeneMapper 3.7 (Applied Biosystems), GenoProfiler 2.0 , and uploaded to FPC v8.5.3 software  for contig assembly. To maintain the quality of contig assembly the initial build was processed at high stringency using the cutoff of 1e-50 and a tolerance of 3. The DQer function of the FPC package was performed to break down all contigs with more than 10% of Q clones to reduce false assembly. Further reassembly was conducted by consecutive reductions of the stringency at 1e-5 for the Ends-Ends analysis followed by Single-End analysis until the final cutoff of 1e-35 with tolerance of 3 was reached. The accuracy of the contig assembly was examined by marker hybridization and PCR analysis. Further manual editing of the assembly was conducted based on the following principles: (a) cutoff at 1e-20 and tolerance at 3, (b) for 2 contigs to be merged, the first contig needs to have at least 3 matched clones (matched clones are clones shared at least 41 common bands under the designated stringency) and the second contig needs to have at least 2 matched clones, (c) only 2 matched clones are required for contig merge if these 2 contigs also share the same genetic marker(s),
Overgo design and hybridization
To establish a genome resource from an environmental and ecological model plant to better support gene identification and characterization, a collection of stress-induced genes were first chosen for hybridization to anchor the potential stress-related markers in the physical map. Furthermore, a BAC library from A. coerulea was also included in the hybridization for comparative genomics studies. Briefly, ESTs preferentially up-regulated by drought-stress were generated from subtractive hybridization analysis (Henry, unpublished data). The low complex sequences were further removed by a pipeline composed of Repeat Masker  with the RepBase database , Cross_Match  and Tandem Repeat Finder . The remaining sequences were screened for overgo oligomers by OligoSpawn . A total of 125 pairs of oligomers were synthesized by IDT (Integrated DNA Technologies). Overgo probes were individually labelled by following the procedure of the Clemson University Genomics Institute (CUGI) hybridization protocol http://www.genome.clemson.edu/resources/protocols. An in-house experimental design script http://www.genome.clemson.edu/software/hybdecon/exp_setup was used to assign probes into 15 pools in a 3-dimensional pooling design, with each pool containing 25 probes (Figure 1). All 32-P labelled probes were mixed in their corresponding pools, denatured and added to hybridization against 2 BAC libraries, including the AF_Bb library of A. formosa and a Hind III library of A. coerulea. Hybridization was performed at 60°C for 2 nights. Filters were washed with 1× SSC, 0.1% SDS at 60°C for 30 minutes for 5 times and exposed to phosphor screens, and the images were recorded by a Typhoon 9400 Imager (GE Healthcare, Bio-Sciences). The addresses of the positively hybridized BAC clones were manually scored using the software HybSweeper , and subsequently deconvoluted for positive BACs corresponding to each probe with an in-house PERL script Hybdecon http://www.genome.clemson.edu/software/hybdecon. Hybridization results were then incorporated into FPC project to anchor markers into the contig framework.
By following the same procedure, another set of 125 overgo probes was designed from various resources, including 87 mapped markers, 16 genes potentially involved in anthocyanin biosynthesis , 12 genes involved in floral development (Kramer, unpublished data) and 10 other SNP markers for additional pool hybridization. Successful markers were integrated into the map. Sequence information of all overgo probes were listed in Additional file 3.
Contig validation by marker hybridization and PCR analysis
For PCR validation, primers were designed from a total of 8 markers randomly chosen from the drought-stress induced ESTs (Table 5). All positively hybridized BACs corresponding to every individual marker were analyzed by PCR amplification. The condition for the PCR reaction was 94°C for 1 min for initial denaturation, followed by 25 cycles of denaturing at 94°C for 15 sec, annealing at 55°C for 30 sec, and extension at 60°C for 60 sec, followed by a final cycle of extension for 10 min. The reagents were PCR kit from Clonetech (Palo Alto, CA). The amplicons were resolved in 1.0% agarose gel and ethidium bromide stained, and the presence/absence of the amplicons of expected sizes were examined.
BAC end sequencing
A total of 6,505 overlapping BAC clones that constituted the minimal tiling path were rearrayed and cultured in 96-well deep plates for DNA isolation, and approximately 300 ng of each individual DNA was used for BAC end sequencing by universal T7 and Sp6 primers for both ends using the "Dye Terminator" chemistry from ABI kit version v3.1 and resolved on ABI3730XL sequencer. In-house quality control software was used to filter and trim raw sequences. The pipeline includes publicly available tools such as Phred , Cross_Match  and Lucy  for base calling and vector masking. Trimmed sequences of less than 100 bp or with greater than 5% N bases were removed. The high quality, trimmed sequences were searched for organelle origin by BLAST  against multiple genomes from GenBank: A. thaliana, Nicotiana sylvestris, O. sativa, and Ranunculus macranthus chloroplast genomes and the A. thaliana, N. tobacum, O. sativa and V. vinifera mitochondrial genomes. The software RepeatMasker version 3.2.7  coupling with a RepBase library  of all known Viridiplantae repetitive elements was used to identify repeats from the Aquilegia BESs. Classification of the repeat families was based on the annotation in the database. A CUGI PERL script was used to identify microsatellites with at least five dinucleotide, four trinucleotide, three tetranucleotide or three pentanucleotide motifs in a row. Primer3  was used to identify primers surrounding each predicted SSR element.
BAC-end sequences anchored to fingerprint contigs were assessed for synteny with the V. vinifera draft genome assembly http://www.plantgdb.org/VvGDB using the SyMAP  software. First, repetitive/low-complex motifs were screened and masked with Repeatmasker . Next, BLAT  was used to align the FPC sequences (BES and markers) using the nucleotide/nucleotide search mode with a minScore of 30 and a minIdentity of 70.
This project was based upon work supported by the National Science Foundation grant EF-0412727 to SAH, JPT and HL and a UCSB faculty research grant to SAH, and in part by a grant from NIFA/USDA, under project number SC-1700315 to HL. The authors also thank Dr. Elena Kramer who provided the floral development markers. This is technical contribution no. 5811 of the Clemson Experiment Station.
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