Articles

DNA methylation analysis of porcine mammary epithelial cells reveals differentially methylated loci associated with immune response against Escherichia coli challenge

Epigenetic changes such as cytosine (CpG) DNA methylations regulate gene expression patterns in response to environmental cues including infections. Microbial infections induce DNA methylations that play a pot...

Authors: Basavaraj Sajjanar, Nares Trakooljul, Klaus Wimmers and Siriluck Ponsuksili

Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows

Genetic polymorphisms are known to influence milk production and composition. However, the genomic mechanisms involved in the genetic regulation of milk component synthesis are not completely understood. Micro...

Authors: P. A. Billa, Y. Faulconnier, T. Ye, M. Chervet, F. Le Provost, J. A. A. Pires and C. Leroux

Comparison of gene expression profile of the spinal cord of sprouting-capable neonatal and sprouting-incapable adult mice

The regenerative ability of severed axons in the central nervous system is limited in mammals. However, after central nervous system injury, neural function is partially recovered by the formation of a compens...

Authors: Hiroshi Tsujioka and Toshihide Yamashita

Estimation of a significance threshold for genome-wide association studies

Selection of an appropriate statistical significance threshold in genome-wide association studies is critical to differentiate true positives from false positives and false negatives. Different multiple testin...

Authors: Avjinder S. Kaler and Larry C. Purcell

Whole genome sequence and de novo assembly revealed genomic architecture of Indian Mithun (Bos frontalis)

Mithun (Bos frontalis), also called gayal, is an endangered bovine species, under the tribe bovini with 2n = 58 XX chromosome complements and reared under the tropical rain forests region of India, China, Myanmar...

Authors: Sabyasachi Mukherjee, Zexi Cai, Anupama Mukherjee, Imsusosang Longkumer, Moonmoon Mech, Kezhavituo Vupru, Kobu Khate, Chandan Rajkhowa, Abhijit Mitra, Bernt Guldbrandtsen, Mogens Sandø Lund and Goutam Sahana

An insight into the sialome, mialome and virome of the horn fly, Haematobia irritans

The horn fly (Haematobia irritans) is an obligate blood feeder that causes considerable economic losses in livestock industries worldwide. The control of this cattle pest is mainly based on insecticides; unfortun...

Authors: J. M. Ribeiro, Humberto Julio Debat, M. Boiani, X. Ures, S. Rocha and M. Breijo

The genome-wide impact of cadmium on microRNA and mRNA expression in contrasting Cd responsive wheat genotypes

Heavy metal ATPases (HMAs) are responsible for Cd translocation and play a primary role in Cd detoxification in various plant species. However, the characteristics of HMAs and the regulatory mechanisms between...

Authors: Min Zhou, Shigang Zheng, Rong Liu, Lu Lu, Chihong Zhang, Lei Zhang, Levi Yant and Yu Wu

Cut-C: cleavage under tethered nuclease for conformational capture

Deciphering the 3D structure of the genome is essential for elucidating the regulatory mechanisms of gene expression in detail. Existing methods, such as chromosome conformation capture (3C) and Hi-C have enab...

Authors: Takashi Shimbo, Machika Kawamura, Edward Wijaya, Eiichi Takaki, Yasufumi Kaneda and Katsuto Tamai

Promoter conservation in HDACs points to functional implications

Histone deacetylases (HDACs) are the proteins responsible for removing the acetyl group from lysine residues of core histones in chromosomes, a crucial component of gene regulation. Eleven known HDACs exist in...

Authors: Toni A. Boltz, Sawsan Khuri and Stefan Wuchty

Genome-wide analysis of the NF-Y gene family in peach (Prunus persica L.)

Nuclear Factor Y (NF-Y) is a heterotrimeric complex composed of three unique subunits: NF-YA, NF-YB, and NF-YC. The NF-Y transcription factor complex binds to the CCAAT box of eukaryotic promoters, playing a vita...

Authors: Miao Li, Guixiang Li, Wei Liu, Xiaomin Dong and Anning Zhang

Single-cell RNA-seq of esophageal squamous cell carcinoma cell line with fractionated irradiation reveals radioresistant gene expression patterns

Esophageal squamous cell carcinoma (ESCC) cells are heterogeneous, easily develop radioresistance, and recur. Single-cell RNA-seq (scRNA-seq) is a next-generation sequencing method that can delineate diverse g...

Authors: Ling Yang, Xiaoyan Zhang, Qiang Hou, Ming Huang, Hongfang Zhang, Zhenzhen Jiang, Jing Yue and Shixiu Wu

Small RNA discovery in the interaction between barley and the powdery mildew pathogen

Plants encounter pathogenic and non-pathogenic microorganisms on a nearly constant basis. Small RNAs such as siRNAs and miRNAs/milRNAs influence pathogen virulence and host defense responses. We exploited the ...

Authors: Matt Hunt, Sagnik Banerjee, Priyanka Surana, Meiling Liu, Greg Fuerst, Sandra Mathioni, Blake C. Meyers, Dan Nettleton and Roger P. Wise

Insertion/deletion-activated frame-shift fluorescence protein is a sensitive reporter for genomic DNA editing

Reporter methods to quantitatively measure the efficiency and specificity of genome editing tools are important for the development of novel editing techniques and successful applications of available ones. Ho...

Authors: Akhilesh Kumar, Michael D. Birnbaum, Balaji T. Moorthy, Jayanti Singh, Anna Palovcak, Devang M. Patel and Fangliang Zhang

How do Wolbachia modify the Drosophila ovary? New evidences support the “titration-restitution” model for the mechanisms of Wolbachia-induced CI

Cytoplasmic incompatibility (CI) is the most common phenotype induced by endosymbiont Wolbachia and results in embryonic lethality when Wolbachia-modified sperm fertilize eggs without Wolbachia. However, eggs car...

Authors: Zhen He, Ya Zheng, Wen-Juan Yu, Yang Fang, Bin Mao and Yu-Feng Wang

Comparative transcriptomics suggest unique molecular adaptations within tardigrade lineages

Tardigrades are renowned for their ability to enter cryptobiosis (latent life) and endure extreme stress, including desiccation and freezing. Increased focus is on revealing molecular mechanisms underlying thi...

Authors: Maria Kamilari, Aslak Jørgensen, Morten Schiøtt and Nadja Møbjerg

Genome-wide localization of the polyphenol quercetin in human monocytes

Quercetin is a polyphenol of great interest given its antioxidant activity and involvement in the immune response. Although quercetin has been well studied at the molecular level as a gene regulator and an act...

Authors: Dana Atrahimovich, Avraham O. Samson, Yifthah Barsheshet, Jacob Vaya, Soliman Khatib and Eli Reuveni

The lichen symbiosis re-viewed through the genomes of Cladonia grayi and its algal partner Asterochloris glomerata

Lichens, encompassing 20,000 known species, are symbioses between specialized fungi (mycobionts), mostly ascomycetes, and unicellular green algae or cyanobacteria (photobionts). Here we describe the first para...

Authors: Daniele Armaleo, Olaf Müller, François Lutzoni, Ólafur S. Andrésson, Guillaume Blanc, Helge B. Bode, Frank R. Collart, Francesco Dal Grande, Fred Dietrich, Igor V. Grigoriev, Suzanne Joneson, Alan Kuo, Peter E. Larsen, John M. Logsdon Jr, David Lopez, Francis Martin…

Impact of sequencing depth and technology on de novo RNA-Seq assembly

RNA-Seq data is inherently nonuniform for different transcripts because of differences in gene expression. This makes it challenging to decide how much data should be generated from each sample. How much shoul...

Authors: Jordan Patterson, Eric J. Carpenter, Zhenzhen Zhu, Dan An, Xinming Liang, Chunyu Geng, Radoje Drmanac and Gane Ka-Shu Wong

Pea genomic selection for Italian environments

A thorough verification of the ability of genomic selection (GS) to predict estimated breeding values for pea (Pisum sativum L.) grain yield is pending. Prediction for different environments (inter-environment pr...

Authors: Paolo Annicchiarico, Nelson Nazzicari, Luciano Pecetti, Massimo Romani and Luigi Russi

Comparative genomic analysis revealed great plasticity and environmental adaptation of the genomes of Enterococcus faecium

As an important nosocomial pathogen, Enterococcus faecium has received increasing attention in recent years. However, a large number of studies have focused on the hospital-associated isolates and ignored isolate...

Authors: Zhi Zhong, Lai-Yu Kwok, Qiangchuan Hou, Yaru Sun, Weicheng Li, Heping Zhang and Zhihong Sun

Prediction of regulatory long intergenic non-coding RNAs acting in trans through base-pairing interactions

Long intergenic non-coding RNAs (lincRNAs) can act as regulators of expression of protein-coding genes. Trans-natural antisense transcripts (trans-NATs) are a type of lincRNAs that contain sequence complementary ...

Authors: Jules Deforges, Rodrigo S. Reis, Philippe Jacquet, Dominique Jacques Vuarambon and Yves Poirier

Transcriptome profiling of Gerbera hybrida reveals that stem bending is caused by water stress and regulation of abscisic acid

Gerbera hybrida is one of the most popular cut flowers in the world; however, stem bending, which always happens when gerbera flower harvested from the field, greatly limits its vase life. To date the molecular m...

Authors: Yafei Ge, Qixian Lai, Ping Luo, Xiaojing Liu and Wen Chen

A high density SLAF-seq SNP genetic map and QTL for seed size, oil and protein content in upland cotton

Cotton is a leading natural fiber crop. Beyond its fiber, cottonseed is a valuable source of plant protein and oil. Due to the much higher value of cotton fiber, there is less consideration of cottonseed quali...

Authors: Wenwen Wang, Ying Sun, Peng Yang, Xiaoyan Cai, Le Yang, Junrui Ma, Yuncan Ou, Tianpeng Liu, Iftikhar Ali, Dajun Liu, Jian Zhang, Zhonghua Teng, Kai Guo, Dexin Liu, Fang Liu and Zhengsheng Zhang

Genome survey and high-resolution backcross genetic linkage map construction of the ridgetail white prawn Exopalaemon carinicauda applications to QTL mapping of growth traits

High-resolution genetic linkage map is critical for QTL mapping, genome sequence assembly and marker-assisted selection in aquaculture species. The ridgetail white prawn Exopalaemon carinicauda is one of the most...

Authors: Jitao Li, Jianjian Lv, Ping Liu, Ping Chen, Jiajia Wang and Jian Li

Cold seawater induces early sexual developmental stages in the BPG axis of European eel males

The impossibility of closing the life cycle of the European eel (Anguilla anguilla) in captivity troubles the future of this critically endangered species. In addition, the European eel is a highly valued and dem...

Authors: Christoffer Rozenfeld, Víctor García-Carpintero, Luz Pérez, Victor Gallego, Juan Germán Herranz-Jusdado, Helge Tveiten, Helge K. Johnsen, Romain Fontaine, Finn-Arne Weltzien, Joaquín Cañizares, Juan F. Asturiano and David S. Peñaranda

Uncovering the molecular signature underlying the light intensity-dependent root development in Arabidopsis thaliana

Root morphology is known to be affected by light quality, quantity and direction. Light signal is perceived at the shoot, translocated to roots through vasculature and further modulates the root development. P...

Authors: Sony Kumari, Sandeep Yadav, Debadutta Patra, Sharmila Singh, Ananda K. Sarkar and Kishore C. S. Panigrahi

Discovery and characterization of the evolution, variation and functions of diversity-generating retroelements using thousands of genomes and metagenomes

Diversity-generating retroelements (DGRs) are a unique family of retroelements that generate sequence diversity of DNA to benefit their hosts by introducing variations and accelerating the evolution of target ...

Authors: Fazhe Yan, Xuelin Yu, Zhongqu Duan, Jinyuan Lu, Ben Jia, Yuyang Qiao, Chen Sun and Chaochun Wei

Genome and transcriptome characterization of the glycoengineered Nicotiana benthamiana line ΔXT/FT

The allotetraploid tobacco species Nicotiana benthamiana native to Australia has become a popular host for recombinant protein production. Although its usage grows every year, little is known on this plant’s geno...

Authors: Matteo Schiavinato, Richard Strasser, Lukas Mach, Juliane C. Dohm and Heinz Himmelbauer

Gene expression profile of human T cells following a single stimulation of peripheral blood mononuclear cells with anti-CD3 antibodies

Anti-CD3 immunotherapy was initially approved for clinical use for renal transplantation rejection prevention. Subsequently, new generations of anti-CD3 antibodies have entered clinical trials for a broader sp...

Authors: Isabel Garcia Sousa, Kelly Cristina Rodrigues Simi, Manuela Maragno do Almo, Maryani Andressa Gomes Bezerra, Gero Doose, Tainá Raiol, Peter F. Stadler, Steve Hoffmann, Andréa Queiroz Maranhão and Marcelo Macedo Brigido

Honey bee (Apis mellifera) larval pheromones may regulate gene expression related to foraging task specialization

Foraging behavior in honey bees (Apis mellifera) is a complex phenotype that is regulated by physiological state and social signals. How these factors are integrated at the molecular level to modulate foraging be...

Authors: Rong Ma, Juliana Rangel and Christina M. Grozinger

geneHummus: an R package to define gene families and their expression in legumes and beyond

During the last decade, plant biotechnological laboratories have sparked a monumental revolution with the rapid development of next sequencing technologies at affordable prices. Soon, these sequencing technolo...

Authors: Jose V. Die, Moamen M. Elmassry, Kimberly H. LeBlanc, Olaitan I. Awe, Allissa Dillman and Ben Busby

The first evidence of global meat phosphoproteome changes in response to pre-slaughter stress

Pre-slaughter stress (PSS) impairs animal welfare and meat quality. Dark, firm and dry (DFD) are terms used to designate poor quality meats induced by PSS. Protein phosphorylation can be a potentially signific...

Authors: Ariadna Mato, Raquel Rodríguez-Vázquez, María López-Pedrouso, Susana Bravo, Daniel Franco and Carlos Zapata

Transcriptome profiling of Puccinellia tenuiflora during seed germination under a long-term saline-alkali stress

Puccinellia tenuiflora is the most saline-alkali tolerant plant in the Songnen Plain, one of the three largest soda saline-alkali lands worldwide. Here, we investigated the physicochemical properties of saline-al...

Authors: Xiaoxue Ye, Hao Wang, Xiuling Cao, Xuejiao Jin, Fuqiang Cui, Yuanyuan Bu, Hua Liu, Wenwu Wu, Tetsuo Takano and Shenkui Liu

Analysis of the equine “cumulome” reveals major metabolic aberrations after maturation in vitro

Maturation of oocytes under in vitro conditions (IVM) results in impaired developmental competence compared to oocytes matured in vivo. As oocytes are closely coupled to their cumulus complex, elucidating aber...

Authors: Jasmin Walter, Fabian Huwiler, Claudia Fortes, Jonas Grossmann, Bernd Roschitzki, Junmin Hu, Hanspeter Naegeli, Endre Laczko and Ulrich Bleul

Effects of short-time exposure to atrazine on miRNA expression profiles in the gonad of common carp (Cyprinus carpio)

Atrazine is widely used in agriculture and is a known endocrine disrupting chemical. Atrazine can seep into the water body through surface, posing a potential threat to the aquatic ecological environment and h...

Authors: Fang Wang, Qian-wen Yang, Wen-Jie Zhao, Qi-Yan Du and Zhong-Jie Chang

Regulation of terpenoid biosynthesis by miRNA in Persicaria minor induced by Fusarium oxysporum

Persicaria minor (kesum) is an herbaceous plant with a high level of secondary metabolite compounds, particularly terpenoids. These terpenoid compounds have well-established roles in the pharmaceutical and food i...

Authors: Abdul Fatah A. Samad, Reyhaneh Rahnamaie-Tajadod, Muhammad Sajad, Jaeyres Jani, Abdul Munir Abdul Murad, Normah Mohd Noor and Ismanizan Ismail

Systematic analysis of the lysine succinylome in the model medicinal mushroom Ganoderma lucidum

Ganoderma lucidum, one of the best-known medicinal mushrooms in the world, produces more than 400 different bioactive compounds. However, the regulation of these bioactive compounds biosynthesis is still unclear....

Authors: Guangyuan Wang, Lili Xu, Hao Yu, Jie Gao and Lizhong Guo

Relation between DNA ionization potentials, single base substitutions and pathogenic variants

It is nowadays clear that single base substitutions that occur in the human genome, of which some lead to pathogenic conditions, are non-random and influenced by their flanking nucleobase sequences. However, d...

Authors: Fabrizio Pucci and Marianne Rooman

VarI-COSI 2018: a forum for research advances in variant interpretation and diagnostics

Authors: Yana Bromberg, Emidio Capriotti and Hannah Carter

Assessing cell-specific effects of genetic variations using tRNA microarrays

By definition, effect of synonymous single-nucleotide variants (SNVs) on protein folding and function are neutral, as they alter the codon and not the encoded amino acid. Recent examples indicate tissue-specif...

Authors: Christine Polte, Daniel Wedemeyer, Kathryn E. Oliver, Johannes Wagner, Marcel J. C. Bijvelds, John Mahoney, Hugo R. de Jonge, Eric J. Sorscher and Zoya Ignatova

PhenPath: a tool for characterizing biological functions underlying different phenotypes

Many diseases are associated with complex patterns of symptoms and phenotypic manifestations. Parsimonious explanations aim at reconciling the multiplicity of phenotypic traits with the perturbation of one or ...

Authors: Giulia Babbi, Pier Luigi Martelli and Rita Casadio

Benchmarking subcellular localization and variant tolerance predictors on membrane proteins

Membrane proteins constitute up to 30% of the human proteome. These proteins have special properties because the transmembrane segments are embedded into lipid bilayer while extramembranous parts are in differ...

Authors: Tommaso Orioli and Mauno Vihinen

Recurrent miscalling of missense variation from short-read genome sequence data

Short-read resequencing of genomes produces abundant information of the genetic variation of individuals. Due to their numerous nature, these variants are rarely exhaustively validated. Furthermore, low levels...

Authors: Matthew A. Field, Gaetan Burgio, Aaron Chuah, Jalila Al Shekaili, Batool Hassan, Nashat Al Sukaiti, Simon J. Foote, Matthew C. Cook and T. Daniel Andrews

A clinically validated whole genome pipeline for structural variant detection and analysis

With the continuing decrease in cost of whole genome sequencing (WGS), we have already approached the point of inflection where WGS testing has become economically feasible, facilitating broader access to the ...

Authors: Nir Neerman, Gregory Faust, Naomi Meeks, Shira Modai, Limor Kalfon, Tzipora Falik-Zaccai and Alexander Kaplun

What makes a bad egg? Egg transcriptome reveals dysregulation of translational machinery and novel fertility genes important for fertilization

Egg quality can be defined as the egg ability to be fertilized and subsequently develop into a normal embryo. Previous research has shed light on factors that can influence egg quality. Large gaps however rema...

Authors: Caroline T. Cheung, Thao-vi Nguyen, Aurélie Le Cam, Amélie Patinote, Laurent Journot, Christelle Reynes and Julien Bobe

Sex-specific changes in gene expression in response to estrogen pollution around the onset of sex differentiation in grayling (Salmonidae)

The synthetic 17α-ethinylestradiol (EE2) is a common estrogenic pollutant that has been suspected to affect the demography of river-dwelling salmonids. One possibility is that exposure to EE2 tips the balance ...

Authors: Oliver M. Selmoni, Diane Maitre, Julien Roux, Laetitia G. E. Wilkins, Lucas Marques da Cunha, Etienne L. M. Vermeirssen, Susanne Knörr, Marc Robinson-Rechavi and Claus Wedekind

Development of a large SNPs resource and a low-density SNP array for brown trout (Salmo trutta) population genetics

The brown trout (Salmo trutta) is an economically and ecologically important species for which population genetic monitoring is frequently performed. The most commonly used genetic markers for this species are mi...

Authors: Keoni Saint-Pé, Maeva Leitwein, Laurence Tissot, Nicolas Poulet, Bruno Guinand, Patrick Berrebi, Geoffrey Marselli, Jean-Marc Lascaux, Pierre-Alexandre Gagnaire and Simon Blanchet

Transcriptional characterisation of the Exaiptasia pallida pedal disc

Biological adhesion (bioadhesion), enables organisms to attach to surfaces as well as to a range of other targets. Bioadhesion evolved numerous times independently and is ubiquitous throughout the kingdoms of ...

Authors: Peter A. Davey, Marcelo Rodrigues, Jessica L. Clarke and Nick Aldred

Screening of genes encoding adhesion factors and biofilm production in methicillin resistant strains of Staphylococcus aureus isolated from Palestinian patients

Intercellular adhesion and biofilm production by Staphylococcus aureus makes these bacteria resistant to antimicrobial therapy. Here, Methicillin-resistant Staphylococcus aureus (MRSA) strains were characterized ...

Authors: Kifaya Azmi, Walaa Qrei and Ziad Abdeen

Alternative polyadenylation produces multiple 3’ untranslated regions of odorant receptor mRNAs in mouse olfactory sensory neurons

Odorant receptor genes constitute the largest gene family in mammalian genomes and this family has been extensively studied in several species, but to date far less attention has been paid to the characterizat...

Authors: Mohamed Doulazmi, Cyril Cros, Isabelle Dusart, Alain Trembleau and Caroline Dubacq